Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	822,527	C→T	E405K (GAA→AAA)	clsB ←	cardiolipin synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	822,527	0	C	T	100.0%	209.9 / NA	62	E405K (GAA→AAA)	clsB	cardiolipin synthase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/36);  total (26/36)

CGGGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGA  >  NC_000913/822281‑822772                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      cGGGCGAGCAGGTCATAGCAGCCGTAAAGCAGATAGCTGACGACCACCAGCCCGACCGCACCAAGCAGCGCAACGCGATTGGAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTAcc                                                                                                                                                                                                                                                   <  2:723658/251‑1 (MQ=255)    gCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGa                                                                                                                                                                                                                                                <  2:224271/251‑1 (MQ=255)        gCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCt                                                                                                                                                                                                                                            <  1:17000/251‑1 (MQ=255)             tCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGGGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGGGATTTACTCATCAGGGTTTTACCCCCGGGTTTTCAGTTTTTACCCGATCCTGCGtt                                                                                                                                                                                                                                       >  2:368386/1‑251 (MQ=255)                       cGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCg                                                                                                                                                                                                                             <  1:322473/251‑1 (MQ=255)                             ccAGATAGCTGACGACCCCCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTtgtg                                                                                                                                                                                                                       <  2:281904/250‑1 (MQ=255)                                 aTAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGGTAAAAAACAGCCAGGGGAGGATCTTATTTGCTCAGCGCCAGAGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTTTTTTCAGTTTTTACCCGATCCCGCGTTTCCATGGACGGTTGTgtggg                                                                                                                                                                                                                   >  2:670611/1‑251 (MQ=255)                                    gCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTACACTTTTTTGGCGCAGACCACCAGCAACACCATCACCGCGATAAAAAACAGCCAGGTGAGGAACTTCTTTGCTTAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGGTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTgcgggg                                                                                                                                                                                                                  >  2:151174/1‑248 (MQ=255)                                                       aacgtaCTAACCAGCGCAACCCGATTGTATTCCCGGATGACCTTCCAGACCTCTCCCCAGTCCACGTTTTTGGCGTAGACCACCAGCAACACAATCACCGCTATAAAAATCAGCCAGTTGAGGATCTTCTTTGCTAAGCTCCAGCTCGGGTTTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCTGTTGTGCGGGCGGATCAACATGCGCCAGACg                                                                                                                                                                                              <  2:494357/245‑1 (MQ=255)                                                        ccGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACAACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCCGACGTg                                                                                                                                                                                            >  1:816188/1‑251 (MQ=255)                                                               aaGCAGCGCAACGCGATTGTAGTCGCGGCTGACATTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGGGGGCGGATCAACCTGCGCCAGACGTGGCgtggt                                                                                                                                                                                     >  1:267395/1‑249 (MQ=255)                                                                atcagcGCAACGTGATTGTAGTCGCGGATGACCTTCCAGTCCGCTTCCCAGTCCCCTTTTTTTGCGTAGACCACCAGCAACACAATCACCGCGATAAAAATCAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGTGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGTCGTGGCgtgtgt                                                                                                                                                                                    <  2:591926/249‑1 (MQ=255)                                                                 gcagcGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCTGGCGGATCAACCTGCGCCCGACGTGGCGtgtgtg                                                                                                                                                                                   >  1:77382/1‑251 (MQ=255)                                                                      gtgaagcgtttGTAGTCGCGGATGAACTGCCAGAGCTCTGTCCAGTCCCCGTTTGTGGCGTAGACCTCCAGCAACACAATCACCGCGAGAAAAAACAGCCAGGTGAGGATCGTCTTTGCTAAGCTCCAGCGCGGTTGTGAGTTACTCATCAGGGTTTTACCCCCGTGTTTTAAGTTTTTACCCGATCCTGCGTTTGCATTGTCGGGTGTGCGGGCGGATCAACCTGCGCCATTCGTGGCgtttgggacgga                                                                                                                                                                              <  2:220557/242‑11 (MQ=255)                                                                         aCGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAagc                                                                                                                                                                           <  1:811623/251‑1 (MQ=255)                                                                                 gTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAAc                                                                                                                                                                   <  1:466433/251‑1 (MQ=255)                                                                                      cgcgGATGACCTTCCAGACCTTTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAgc                                                                                                                                                               <  1:602388/250‑1 (MQ=255)                                                                                      cgcgGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAgcg                                                                                                                                                              <  1:47264/251‑1 (MQ=255)                                                                                        cgGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGcc                                                                                                                                                            <  1:422413/251‑1 (MQ=255)                                                                                          gATGACCTTCCAGACCTCTTCCCAGGCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCgg                                                                                                                                                          <  1:577514/251‑1 (MQ=255)                                                                                              aCCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTACCGGGAGCCAGCCAACCAGCGCCGGGacg                                                                                                                                                      >  2:792419/1‑249 (MQ=255)                                                                                               ccctacAGACCTCTTCCCAGTCCTATTTCTTGGCTTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGt                                                                                                                                                     <  2:260415/246‑1 (MQ=255)                                                                                                     aGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGAGCCGGGAAGTGGCGt                                                                                                                                                >  1:775754/1‑250 (MQ=255)                                                                                                        ccTCTTCCCAGGCCACTTTTTGGGCGTAGACCCCCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTaaaa                                                                                                                                            <  2:693549/251‑1 (MQ=255)                                                                                                                cAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTTCTCATCAGGGTTTTTCCCCCGTGTTTTCAGTTTTTACCCGAGCCTGCGTTTCCATTGTCGGTGGTGCGGGCGGATCAGCCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAAc                                                                                                                                    <  1:741007/251‑1 (MQ=255)                                                                                                                              ggCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCtttt                                                                                                                      <  1:220808/251‑1 (MQ=255)                                                                                                                              ggCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCCGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCttt                                                                                                                       <  1:670611/250‑1 (MQ=255)                                                                                                                                cGTAGACCACCAGCAACACAAGCACCGCGAGAAAAAACAGCCCGCTGAGGAGCTGCTTTGCTAAGAGCCAGCGCGGGTGTGATTTACTCATCAGGGTTGTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTTTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACTTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTgg                                                                                                                    <  2:176737/251‑1 (MQ=255)                                                                                                                                 gTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGCGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGCTTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGGAGCCAGCCAACCAGCGCCGGGAAGGGGGGTAAAAAGTGGAACGCCAGCACGCTTTTggg                                                                                                                   >  2:670181/1‑250 (MQ=255)                                                                                                                                   aGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGGGGCGTAAAAAGGGGAACGCCAGCACGCTTTTGGTc                                                                                                                  >  1:559228/1‑250 (MQ=255)                                                                                                                                     accaccAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTGAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAgg                                                                                                               <  1:151174/251‑1 (MQ=255)                                                                                                                                     accaccAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGGAGCCAGCCAACCAGCGCCGGGAAGGGGGGGAAAAAGGGGAACGCCAGCACGCTTTTGGTccgg                                                                                                               >  2:417139/1‑248 (MQ=255)                                                                                                                                       caccaGAAACACAATCACCGCGCTACAAAACAGCCAGGTGAGGATCTTCTTTCCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTCCGGGCGGATCAACATGCGCCCACCGTGGCGCGGGTGCCAGAAGCCGGCCCACCAGCGACGGGAAGTGGCGTAAAAAGTGGACCCCCAGCACGCTTTTGGTCAgggt                                                                                                             >  2:296494/1‑249 (MQ=255)                                                                                                                                           aGCAACACAATCCCCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTTATTTACTCATCCGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTccac                                                                                                         <  2:278833/251‑1 (MQ=255)                                                                                                                                            gCAACACAATCGCCGCGATAAAAACCAGCCAGGTGAGTATCTTCTTTGCTAAGCGCCAGCGCGGGGGTGATGTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGGCGGGTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGGGGAACGCCAGCTCGCTTTTGGTCAGCTGccacc                                                                                                        <  2:816188/251‑1 (MQ=255)                                                                                                                                                cacaATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTccacccggg                                                                                                    >  1:433275/1‑247 (MQ=255)                                                                                                                                                 acaATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCCACCCACGCCGGGCAGGGGCGTCACAAGGAGAACCCCAGCACGCATTTGGGCAGGtgccccccgggg                                                                                                   >  2:498885/1‑240 (MQ=255)                                                                                                                                                 acaAGCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTg                                                                                                   <  1:76028/251‑1 (MQ=255)                                                                                                                                                       aCCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTg                                                                                                                                                    <  2:419321/196‑1 (MQ=255)                                                                                                                                                       aCCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTg                                                                                                                                                    >  1:419321/1‑196 (MQ=255)                                                                                                                                                        ccGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTgg                                                                                            <  1:586512/251‑1 (MQ=255)                                                                                                                                                           ggaaaaaaaaCAGCCAGGTGAGGTTCCTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCAGTGGCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGgca                                                                                         <  2:347167/247‑1 (MQ=255)                                                                                                                                                                     aaGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGAGTGTGCCGGAAGCCCGCCAACCAGCGCCGGGGAGTGGCGGAAAAAGGGGAACGCCAGCCCGACTTTGGGCAGGTTCCACCAGGTGCGATTGGGCAGCATGGTTTc                                                                               >  2:607068/2‑251 (MQ=255)                                                                                                                                                                           ggTGAGGTTCGTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTGCGCAGCAGGGTGTTCCCCCCTTGTTTTCAGTGTTTACCCGCTCCTGCGTTTCCAGTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGGGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGCGCGTTTGGGCAGCATGGTTTCATCCAc                                                                         <  2:322808/251‑1 (MQ=255)                                                                                                                                                                           ggTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCAc                                                                         <  1:417139/251‑1 (MQ=255)                                                                                                                                                                             tGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACct                                                                       >  1:243136/1‑251 (MQ=255)                                                                                                                                                                                      tcttTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACctgctg                                                                   <  1:710621/246‑1 (MQ=255)                                                                                                                                                                                                  gcCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGGCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCaata                                                   >  1:394352/1‑250 (MQ=255)                                                                                                                                                                                                       cgcgGTTGTGATTTACTCATCAGGGTTCTCCCCCCGTGTTTTCAGTTTTTCCCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCg                                             <  2:559228/251‑1 (MQ=255)                                                                                                                                                                                                            gtgtGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAg                                        <  1:670181/251‑1 (MQ=255)                                                                                                                                                                                                              gtgATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTTTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGGGGAACGCCAGCCCGCTTTTGGTCCGGTTCCACCCGGTGCGTTTTGGCAGCCTGGTTTCATCCACCTGATGACCATCTGCGGCAATAATGCCGTTc                                          >  2:750788/1‑247 (MQ=255)                                                                                                                                                                                                                                 tttACCCCCGTGTTTTCAGTTTTTACCCGATCCTGCGGTTCCATTTTCGGTTTTGCGTGCGGATCAACCTGCTCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTTGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCGCCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGCCTGGt                    <  2:775754/250‑1 (MQ=255)                                                                                                                                                                                                                                       cccGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGTTTATCGCGCAGCGTCTGGTTAAAATg             >  2:745950/1‑251 (MQ=255)                                                                                                                                                                                                                                       cccGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAAGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATg             >  1:645948/1‑251 (MQ=255)                                                                                                                                                                                                                                         cGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGAc           >  1:611238/1‑251 (MQ=255)                                                                                                                                                                                                                                         cGTGTTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTATGGTTAAAATGAc           >  1:649455/1‑251 (MQ=255)                                                                                                                                                                                                                                           tgtTTTCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACg          >  1:574272/1‑250 (MQ=255)                                                                                                                                                                                                                                               ttCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCg                          <  1:132711/230‑1 (MQ=255)                                                                                                                                                                                                                                               ttCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCCCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCCACCCGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCCGCCCCCTTTTGGTCAGGTTCCACCCGGGGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCCATAATGCCGTTCAGGTTTTCCCGCAGCgagatctgaagagcgtagggtg     >  2:132711/1‑230 (MQ=255)                                                                                                                                                                                                                                                tCAGTTTTTCCCCGATCCGGCGTTTCCAGTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGt     <  1:351849/250‑1 (MQ=255)                                                                                                                                                                                                                                                tCAGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGGAGTGGCGTAAAAAAGGGAAAGCCAGCCAGCTTTTTGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGAAATAAAGCCGGTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATcctg    >  2:646649/1‑248 (MQ=255)                                                                                                                                                                                                                                                  aGTTTTTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGga  <  1:439208/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      CGGGCGAGCAGGTCATAGCAGCCGTAAATCAGATAGCTGACGACCACCAGCCCGACCGCACTAAGCAGCGCAACGCGATTGTAGTCGCGGATGACCTTCCAGACCTCTTCCCAGTCCACTTTTTTGGCGTAGACCACCAGCAACACAATCACCGCGATAAAAAACAGCCAGGTGAGGATCTTCTTTGCTAAGCGCCAGCGCGGGTGTGATTTACTCATCAGGGTTTTACCCCCGTGTTTTCAGTTTCTACCCGATCCTGCGTTTCCATTGTCGGTTGTGCGGGCGGATCAACCTGCGCCAGACGTGGCGTGTGTGCCGGAAGCCAGCCAACCAGCGCCGGGAAGTGGCGTAAAAAGTGGAACGCCAGCACGCTTTTGGTCAGGTTCCACCAGGTGCGTTTGGGCAGCATGGTTTCATCCACCTGCTGACAATCTGCGGCAATAATGCCGTTCAGATTATCGCGCAGCGTCTGGTTAAAATGACGATCGTGGA  >  NC_000913/822281‑822772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑