Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,433,900	C→T	L71L (CTA→TTA)	def →	peptide deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,433,900	0	C	T	98.6%	225.0 / NA	69	L71L (CTA→TTA)	def	peptide deformylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (35/33);  minor base A (1/0);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGCTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCACCGCTGAAACA  >  NC_000913/3433655‑3434144                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     atcgttCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTTCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAgtg                                                                                                                                                                                                                                                 <  2:712830/247‑1 (MQ=255)    tttCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGtt                                                                                                                                                                                                                                               >  1:467413/1‑250 (MQ=255)      tCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTaat                                                                                                                                                                                                                                            >  1:277081/1‑251 (MQ=255)         aaCTCCTGAACACATCTCTGGAGATTTATGTCAGTGTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTGTCGGAAAACCGTGACGAACGGTTAGTGTTaatc                                                                                                                                                                                                                                           <  2:503893/249‑1 (MQ=255)          acgccTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTaatcaat                                                                                                                                                                                                                                        <  2:21620/248‑1 (MQ=255)           ccccTGAACACATCTCGGGAGATTTTTGTCAGTTTTGCAAGTGTTACAGATTCCGGACGAGCGGCGTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTaatcaatc                                                                                                                                                                                                                                       <  2:535776/249‑1 (MQ=255)           cTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCAGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCCTTGTTATTGATGTTTAGGAAAACCGTGACGAACGGTTAGTGTTaatcaatc                                                                                                                                                                                                                                       >  2:425298/1‑251 (MQ=255)              cTGAACACATCTATGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGCTATCCATCAACGAATCATTGTTATTGATGTTTCGGAAAACCGTGACGCACGGTTAGGGTTAATCAATCCa                                                                                                                                                                                                                                     >  2:454964/1‑250 (MQ=255)                 aaCACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTaatcaat                                                                                                                                                                                                                                        >  1:493749/1‑244 (MQ=255)                 aaCACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGGTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTaatcaat                                                                                                                                                                                                                                        <  2:493749/244‑1 (MQ=255)                                          gTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAgg                                                                                                                                                                                                        >  1:447557/1‑251 (MQ=255)                                               gCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCAtt                                                                                                                                                                                                    <  2:687061/250‑1 (MQ=255)                                               gCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTGTAGAAAAAAGCGGCGAAACAGGCAtt                                                                                                                                                                                                    <  1:531425/250‑1 (MQ=255)                                                 aaGTGTTACATATTCCGGACGGGCGGCTTCGCACAGTTGCTAAACTGGTAGAAGAAGTGATTGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGCTGTACGCAGAAGAAGGTATTGGCCTGGAGGCAAACCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGCACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAAAAGGCATTga                                                                                                                                                                                                  >  2:400110/1‑250 (MQ=255)                                                     gtTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTgaaga                                                                                                                                                                                               >  2:104921/1‑249 (MQ=255)                                                     gtTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTgaaga                                                                                                                                                                                               >  1:691044/1‑249 (MQ=255)                                                       tACAAATTCCGGACGAGCGGCTTTGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGTGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGGATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAgg                                                                                                                                                                                            <  2:625284/250‑1 (MQ=255)                                                        aCATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGtt                                                                                                                                                                                          >  1:629764/1‑251 (MQ=255)                                                        aCATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCGTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGtt                                                                                                                                                                                          <  2:244673/251‑1 (MQ=255)                                                         cATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTg                                                                                                                                                                                         >  1:434936/1‑251 (MQ=255)                                                          atatTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGc                                                                                                                                                                                        >  1:363311/1‑251 (MQ=255)                                                               ccGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGt                                                                                                                                                                                    <  1:104921/250‑1 (MQ=255)                                                                            ttCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGACTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGATGCCTGTCGATCCCTGaaca                                                                                                                                                                       >  2:476139/1‑250 (MQ=255)                                                                                cAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGaacaacttg                                                                                                                                                                  <  2:136056/251‑4 (MQ=255)                                                                                 aaaGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGc                                                                                                                                                                 <  2:791218/251‑1 (MQ=255)                                                                                                 tAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCCACCCAGGTTGATATCCATCCACGTATCATTGTTCTTGATGTTTCGGAAAGCCGTGACGAACGGTTAGTGTGAATCAATCCAGAGCTTTTAGAACAAAGCGGCGAAACAGGCATTGCAGAAGGTTGCCTGTCGATCCCTGAGCAACGTGCTTTAGGGCCgcgcgcc                                                                                                                                                 >  2:801718/1‑250 (MQ=255)                                                                                                  agaagaAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATccc                                                                                                                                                                             >  2:168073/1‑222 (MQ=255)                                                                                                  agaagaAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATccc                                                                                                                                                                             <  1:168073/222‑1 (MQ=255)                                                                                                         gTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGt                                                                                                                                         <  1:454964/251‑1 (MQ=255)                                                                                                             aTGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGAGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTaa                                                                                                                                      >  1:528561/1‑250 (MQ=255)                                                                                                             aTGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGGAGTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTaaa                                                                                                                                     <  2:507851/251‑1 (MQ=255)                                                                                                               gCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTaaaa                                                                                                                                    <  2:226316/250‑1 (MQ=255)                                                                                                                 aGAAATTCAGCGTATCGTCGATGATATTGTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACTAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGCTAAAATTc                                                                                                                                 <  2:551887/251‑1 (MQ=255)                                                                                                                           cGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTgc                                                                                                                       >  1:703798/1‑250 (MQ=255)                                                                                                                            gTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGAc                                                                                                                      >  1:470229/1‑251 (MQ=255)                                                                                                                                        atatGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGCAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACggaca                                                                                                           >  2:27581/1‑247 (MQ=255)                                                                                                                                        atatGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTaaa                                                                                                          <  1:425298/251‑1 (MQ=255)                                                                                                                                          atGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTTATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAAc                                                                                                         <  2:703798/250‑1 (MQ=255)                                                                                                                                                 agaCGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGAGCCCTTGACCGCGACGGTAAACCATTTGa                                                                                                  >  2:203425/1‑250 (MQ=255)                                                                                                                                                              agaagaAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGGGACGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACg                                                                                    >  1:791878/1‑251 (MQ=255)                                                                                                                                                                       gaTTGGCCTGGCGGCAACCCAGGTTGATATCAATCAACGTATCATTGTTATGGATGTTTCGGAAAACCGTGACGAACGGATAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAGGACGGTTGCCTGTCGATACCTGAACAACGTGCTTTAGTGCCGCGAGCAGAGAAAGTTAAAATTCTAGACCTTGACCGCGAAGGTAAAACATTTGAACTGGACGAATACGGTCTGTTat                                                                           >  2:730270/2‑250 (MQ=255)                                                                                                                                                                            gCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATc                                                                      <  2:447557/251‑1 (MQ=255)                                                                                                                                                                            gCCTGGCGGCAACCCAGGTTGATATACATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATc                                                                      <  2:691044/251‑1 (MQ=255)                                                                                                                                                                              cTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTg                                                                    >  1:550135/1‑251 (MQ=255)                                                                                                                                                                                     cAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACTGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATGCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAg                                                             <  2:363311/251‑1 (MQ=255)                                                                                                                                                                                       aCCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGCCCGCGACGGTAACCCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGc                                                            >  2:182275/1‑250 (MQ=255)                                                                                                                                                                                             gatGATATCCATCAACGTAACATTGTTATTGATGTTTCGGAAAACAGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCGTTAGTGCCGCGCGCAGAGAAAGTTAACATTCGCGCCCTTGACAGCGAAGGTAACCCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATg                                                         >  2:210051/3‑247 (MQ=255)                                                                                                                                                                                                gATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGa                                                  <  2:470229/251‑1 (MQ=255)                                                                                                                                                                                                 atatCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAACAGCCGAGCAACAGGCATTGAAGACGGTTGCCTGTCGATCCCTGAACAAAGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAATATTCGCGCCCTTGACCGCGACGGCAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTAAACATGAGTTGGAt                                                 >  1:766123/1‑251 (MQ=255)                                                                                                                                                                                                   atCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATc                                                <  1:711926/250‑1 (MQ=255)                                                                                                                                                                                                     ccATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGACCTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGAt                                                 >  2:86737/1‑247 (MQ=255)                                                                                                                                                                                                     ccATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGAt                                                 <  1:86737/247‑1 (MQ=255)                                                                                                                                                                                                     ccATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCAcc                                             <  2:267700/251‑1 (MQ=255)                                                                                                                                                                                                     ccATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCAcc                                             <  1:347342/251‑1 (MQ=255)                                                                                                                                                                                                      cATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCt                                            <  2:550135/251‑1 (MQ=255)                                                                                                                                                                                                        tCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTATCCATCTGTATTCAGCATGAGATGGATCACCTgg                                          >  1:153451/1‑251 (MQ=255)                                                                                                                                                                                                         cAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCATAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGt                                         >  1:427388/1‑251 (MQ=255)                                                                                                                                                                                                           aCGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCg                                       <  2:277081/251‑1 (MQ=255)                                                                                                                                                                                                             gTATTATTGTTATTGATGATTCGGAAATCCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGc                                     <  2:554378/251‑1 (MQ=255)                                                                                                                                                                                                                   ttGTTATTGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTg                               >  1:176045/1‑251 (MQ=255)                                                                                                                                                                                                                      ttaatGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGGATTCAGAATGAGATGGATCACCTGGTCGGCAAACTGttt                            >  1:433169/5‑251 (MQ=255)                                                                                                                                                                                                                          tGATGTTTCGGAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATg                         <  1:203425/250‑1 (MQ=255)                                                                                                                                                                                                                                   ggAAAACCGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAATGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGt               >  1:755923/1‑251 (MQ=255)                                                                                                                                                                                                                                       aaCCGTGACGAACGGTTAGTGTTAATCAATCCAGCGCTTTTAGAAAAAAGCGGCGACACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCGGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCCTGAGATGGATCACCTGGTCGGCAACCTGTTTATGGTTTATCTGTCAcc           >  2:150577/1‑251 (MQ=255)                                                                                                                                                                                                                                         ccGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCACCGc         >  1:441930/1‑251 (MQ=255)                                                                                                                                                                                                                                          cGTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCACCGCt        >  1:238860/1‑251 (MQ=255)                                                                                                                                                                                                                                           gTGACGAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGCTCCCTGAACAACGTGATTTAGTGCCGCGAGCAGAGAAAGTTAAAATTCACGCCCTTGACCGCGACGGTAAACCATTTGCACTGGAAGCAGACGGTCTCTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGCCGGCAAACTGTTTATGCATTATCTGTCACCGCTg       >  1:362880/1‑251 (MQ=255)                                                                                                                                                                                                                                             gACGAACGGTTAGTGTTAATCAATCCAGAGCTGTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTGGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCACCGCTGa      <  2:434936/250‑1 (MQ=255)                                                                                                                                                                                                                                                gAACGGTTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCACCGCTGAaaca  <  2:441930/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     ATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGCTAGTGTTAATCAATCCAGAGCTTTTAGAAAAAAGCGGCGAAACAGGCATTGAAGAAGGTTGCCTGTCGATCCCTGAACAACGTGCTTTAGTGCCGCGCGCAGAGAAAGTTAAAATTCGCGCCCTTGACCGCGACGGTAAACCATTTGAACTGGAAGCAGACGGTCTGTTAGCCATCTGTATTCAGCATGAGATGGATCACCTGGTCGGCAAACTGTTTATGGATTATCTGTCACCGCTGAAACA  >  NC_000913/3433655‑3434144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑