Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,247,134 A→C Y467D (TAC→GAC)  lysP ← lysine:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,247,1340AC90.9% 168.7 / 0.6 55Y467D (TAC→GAC) lysPlysine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base C (32/18);  total (36/19)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

AGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGGCAATCCC  >  NC_000913/2246892‑2247371
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             
aGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAAAGATAATGCAAACGATAACAATTATCAATCCAAATGTTAACTTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAAcc                                                                                                                                                                                                                                       >  2:454333/1‑251 (MQ=255)
             tctGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCAAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCCAATAATCAGGac                                                                                                                                                                                                                          >  2:289268/1‑250 (MQ=255)
                gATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATAACCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACa                                                                                                                                                                                                                        <  1:688488/250‑1 (MQ=255)
                 aTTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGc                                                                                                                                                                                                                      <  1:781398/251‑1 (MQ=255)
                          ccTTTTTTGATTGCGGCGTAACGATAATGCAAAAGATAACAATTATCAATCCAAGTGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGGGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGAGGGATAcc                                                                                                                                                                                                              >  1:440114/1‑250 (MQ=255)
                           cTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCa                                                                                                                                                                                                             >  2:743117/1‑250 (MQ=255)
                              ttttGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAatat                                                                                                                                                                                                         >  1:294029/1‑251 (MQ=255)
                                     tGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTg                                                                                                                                                                                                   <  1:634555/250‑1 (MQ=255)
                                           gTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTAc                                                                                                                                                                                            >  1:71319/1‑251 (MQ=255)
                                                   aaTGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGCACCAAATAATCAGGAACAGCGGGATACCAATATAAGTTGCCGCTACGCCGcccc                                                                                                                                                                                    >  2:580759/1‑251 (MQ=255)
                                                    aTGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAAGAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCa                                                                                                                                                                                   <  1:36270/251‑1 (MQ=255)
                                                      gCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAg                                                                                                                                                                                  <  1:413317/250‑1 (MQ=255)
                                                                  aaTTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCtt                                                                                                                                                                     >  1:169875/1‑251 (MQ=255)
                                                                    ttATCAATCCAAATGTTAAATTTTAGATGCGCATGATTTGTCAGCGAAATGGAATTGAAAAAGCCCTCGCGGTTGAGAGGGCTTCGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGCGACCTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATCGTCCGGAACCACGGGATACCAATCTACGTTGCCGCGACGCCGCCCCCGCCAATAGTATCTTTc                                                                                                                                                                   >  2:2025/1‑251 (MQ=255)
                                                                        cAATCCAAATGTTAAATTTTGTATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGACGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAgg                                                                                                                                                                >  2:483078/1‑250 (MQ=255)
                                                                            ccAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATACTCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGc                                                                                                                                                           >  2:320688/1‑251 (MQ=255)
                                                                                 tGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGt                                                                                                                                                      >  2:316576/1‑251 (MQ=255)
                                                                                   ttAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGt                                                                                                                                                      >  2:346818/1‑249 (MQ=255)
                                                                                                  gCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTg                                                                                                                                     >  2:252033/1‑251 (MQ=255)
                                                                                                      gATTTGTCAGGGGAATGGAATTGAAAAAGCACTCTCGGTTGAGAGGGCTTAGCAAGGACGGGAGGAAACTTATTTATTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCCATTAATAAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATTGTATCTTTCAGGAACGCGTGGAAGTTCTTGCCCAAAGTgcta                                                                                                                                  >  2:488720/1‑247 (MQ=255)
                                                                                                                             aaaaaGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAg                                                                                                          <  1:289268/251‑1 (MQ=255)
                                                                                                                              aaaaGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGCATGCGAAgt                                                                                                         >  2:181713/1‑250 (MQ=255)
                                                                                                                                    ccTCTCGGTTGAGAGGGCTTAGCAGGGAAGGGAGGAAACTTATTTCTTCTCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTCTCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGcc                                                                                                   >  2:724999/1‑251 (MQ=255)
                                                                                                                                               agagGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAatat                                                                                                                                                                                                         <  2:494085/138‑1 (MQ=255)
                                                                                                                                               agagGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAatat                                                                                                                                                                                                         >  1:494085/1‑138 (MQ=255)
                                                                                                                                                       tAGCAAGGACGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTg                                                                                 >  1:55827/1‑250 (MQ=255)
                                                                                                                                                              gaaggGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTAc                                                                         >  1:146869/1‑251 (MQ=255)
                                                                                                                                                                  ggaggaAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGc                                                                      >  1:200506/1‑250 (MQ=255)
                                                                                                                                                                  ggaggaAACTTATTTCTTAACGCTCTGCGGGAACTTCATTTCGCGGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATCCCAATATACGTTGCCGCTACGACGCCCCAGGCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGAGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGTTACGGc                                                                      >  2:500148/1‑250 (MQ=255)
                                                                                                                                                                  ggaggaAACTTAGTTCTTAGCGTTCTGCGGGAACTTCATTTCTCTGTAGCGTCCGAAGTGAGTTACTTTAATCAGCTTGACGCCGAACCAAATAATCAGGAACATCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCCGTGGGAAGAATCCTGAACGGTACGGCa                                                                     <  2:659878/251‑1 (MQ=255)
                                                                                                                                                                      gaAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGACTGAATGCGAAGATCGGCCCCAGTGGGAAGAACCCTGAACGGTACgga                                                                      >  2:579988/1‑245 (MQ=255)
                                                                                                                                                                      gaAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATc                                                                 <  2:394179/251‑1 (MQ=255)
                                                                                                                                                                         aattATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAAGCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGtt                                                              <  2:11873/249‑1 (MQ=255)
                                                                                                                                                                             aTTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTAAGAAGTTCGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCAGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGCACGCTTCGTAGTTCTGGCCCAAAGTGATACTCAGACACAGAATGAAAGCGACGATCGGCCCCAGTGGGAAGAAAACTGAACGGTCCGGCAGATAGTTaagg                                                          >  2:660339/1‑249 (MQ=255)
                                                                                                                                                                              tttCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGt                                                         >  2:643084/1‑251 (MQ=255)
                                                                                                                                                                               ttCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTc                                                        >  2:365206/1‑251 (MQ=255)
                                                                                                                                                                                            gCGGGAACTTCCTTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAGTCAGCTTGTCGCCGCACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGATTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCCGTGGGAAGCAACCTGACCGGTACGGCAGATAGTTACTGTCGTGTCCCTGcca                                            >  2:264499/1‑248 (MQ=255)
                                                                                                                                                                                                  aCTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAg                                                                                               >  2:642264/1‑193 (MQ=255)
                                                                                                                                                                                                  aCTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAg                                                                                               <  1:642264/193‑1 (MQ=255)
                                                                                                                                                                                                  aCTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTa                                      >  2:142739/1‑250 (MQ=255)
                                                                                                                                                                                                     tCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAAcc                                   <  1:346818/250‑1 (MQ=255)
                                                                                                                                                                                                     tCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAAc                                    <  2:200506/249‑1 (MQ=255)
                                                                                                                                                                                                      cATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCt                                                                                  >  1:7925/1‑202 (MQ=255)
                                                                                                                                                                                                      cATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACcgc                                 <  1:643084/251‑1 (MQ=255)
                                                                                                                                                                                                                    gTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAg                   <  1:645223/251‑1 (MQ=255)
                                                                                                                                                                                                                      aCGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTg                 >  2:176319/1‑251 (MQ=255)
                                                                                                                                                                                                                          aGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTa             <  1:589075/251‑1 (MQ=255)
                                                                                                                                                                                                                          aGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCt                                                >  2:53903/1‑216 (MQ=255)
                                                                                                                                                                                                                          aGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCt                                                <  1:53903/216‑1 (MQ=255)
                                                                                                                                                                                                                           gTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATCATCAGGCACAGCGGGCTACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCACAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGGAACCGCGAAGGAAGCGATAGTGGCTa             >  1:726990/1‑250 (MQ=255)
                                                                                                                                                                                                                            tGAGTTCCTTTAATCAGCTTGTCGCCGAACCACATAATCAGGAACAGCGGGATACCAATATACGATGCCCCTACGCCGCCCCAGGCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGCCACAGAATGACTGCGAAGATCGGCTCACGTGGGAAGACACCTGCCCGGTACTGAAGATCGTTCATGTGGTGTCCCTGCACTACGTAAACACCCCGGAAGCGATAGtagacaat           >  2:650874/1‑244 (MQ=255)
                                                                                                                                                                                                                              aGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGAAGGAAGCGATAGTGGCTAATgg         >  2:721042/1‑251 (MQ=255)
                                                                                                                                                                                                                               gTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGGc        >  1:727595/1‑251 (MQ=255)
                                                                                                                                                                                                                                 tCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGGCa       <  1:176319/250‑1 (MQ=255)
                                                                                                                                                                                                                                      tAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGGCAATccc  <  1:181713/250‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             
AGCCGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGGCAATCCC  >  NC_000913/2246892‑2247371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑