Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,522,369 G→A P37S (CCG→TCG)  xapR ← DNA‑binding transcriptional activator XapR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,522,3690GA93.5% 149.8 / 0.8 46P37S (CCG→TCG) xapRDNA‑binding transcriptional activator XapR
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (20/23);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

CCGCATAACCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGG  >  NC_000913/2522127‑2522607
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              
ccgcCTAACCGGGCCCATCCCTCCCCACTATGCCGTCCCCACTACGCCGGGTTCAATCCGCCCTGCTCCGCCCCGCCCTATTGGTG‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTGGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCttag                                                                                                                                                                                                                                        <  2:436083/251‑4 (MQ=255)
      atccGGGCGCACCCGGCCCCACATGGCCGTTCCCACTCCGCCGAGTTCAATCCGCCCTGCTTCGCCCCGACCTATTTGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACatat                                                                                                                                                                                                                                  <  1:550028/249‑1 (MQ=255)
                      ccccACATTGCCGTTCCCTCTACGCCGAGTTCAACCCGCCCTGCTTCTCCCCGACCTATTTGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTGGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCGCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCtgctgc                                                                                                                                                                                                                  <  1:363338/251‑1 (MQ=255)
                          ccaTTCCCGTTCCCTCTACGCCGAGTTCAATCCGCCCTGCTGCTCCCCGACCTATTTGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCgg                                                                                                                                                                                                              <  2:156871/250‑1 (MQ=255)
                                ccGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCaaa                                                                                                                                                                                                         >  1:688909/1‑250 (MQ=255)
                                    ccctacttcGCCGAGTTCAACCCCCCCTGCTTCCCCCCGTCCTATTTGTC‑AAAGCCGAGCCAATTCATTAGTAGCATGCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGGGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCa                                                                                                                                                                                                    <  2:359070/243‑1 (MQ=255)
                                         cTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAActct                                                                                                                                                                                               >  1:405359/1‑251 (MQ=255)
                                              ccGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCgct                                                                                                                                                                                                                       <  1:43923/222‑1 (MQ=255)
                                               caggtcaaacccgcctgtcTTCCCCCCGACCTATTTGTT‑CAACCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCt                                                                                                                                                                                         <  2:782840/233‑1 (MQ=255)
                                               cGAGTTCAATCCTGCCTGCTCCACCCCTACCT‑TTTGTTTCAATCCGAGCCAATTCAGTATTAGGATTCACCAGCAATCGACGCGGTTCTTCCATCAAGTTTTTGCCCGCGTGTGACAGTACGACGCGGCGCGAATGTCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCGCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACCCTTCCGCt                                                                                                                                                                                         <  2:47082/251‑1 (MQ=255)
                                                          cccccTGCTTCCCCCCGACCTATTTGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTcc                                                                                                                                                                                            <  2:634455/235‑1 (MQ=255)
                                                          cGCCCTGCTTCTCCCCGACCTATTTGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTcc                                                                                                                                                                                            >  1:634455/1‑237 (MQ=255)
                                                                 cTTCTCCCCGACCTATTTGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACgg                                                                                                                                                                       >  1:810424/1‑251 (MQ=255)
                                                                              tATTTGGT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGACCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGACTAAAAAGCTGCGTGCCGCGTTGCTTTTCCCGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAACTGCAACTCTTCCGCTACGGCAAGAAAATCACGGAGCACCTTa                                                                                                                                                              >  2:119377/1‑247 (MQ=255)
                                                                               aTTTGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGAtc                                                                                                                                                         <  1:146600/251‑1 (MQ=255)
                                                                                     t‑CAACCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTGCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGAtctgttc                                                                                                                                                    <  2:54810/250‑1 (MQ=255)
                                                                                              aGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATAcgc                                                                                                                                           <  1:547042/251‑1 (MQ=255)
                                                                                                     aCATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCAt                                                                                                                                    <  1:769204/251‑1 (MQ=255)
                                                                                                       attattATCATTCACCAGCAATCGACGCGATGCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTGTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATaa                                                                                                                                  <  2:592413/251‑1 (MQ=255)
                                                                                                         tattaGCACTCACCAGCAATCGACTCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAAt                                                                                                                                 <  1:71435/250‑1 (MQ=255)
                                                                                                          attaGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAAtt                                                                                                                                <  1:483570/250‑1 (MQ=255)
                                                                                                            taGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGGAGGCTGAGACATATTTa                                                                                                                                                                                                                               <  1:316164/153‑1 (MQ=255)
                                                                                                            taGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGGAGGCTGAGACATATTTa                                                                                                                                                                                                                               >  2:316164/1‑153 (MQ=255)
                                                                                                              gCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTg                                                                                                                           >  2:168625/1‑251 (MQ=255)
                                                                                                                aTTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATCCGCGTTCCATAATTAAGTGCt                                                                                                                         >  2:538075/1‑251 (MQ=255)
                                                                                                                   cACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTcc                                                                                                                       >  2:210012/1‑250 (MQ=255)
                                                                                                                     ccAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACACTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCat                                                                                                                     >  2:747110/1‑250 (MQ=255)
                                                                                                                          aaTCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCAATataat                                                                                                                >  1:619482/1‑250 (MQ=255)
                                                                                                                              gACGCGATTCTGCCATCAAGATTTTGCCCGCGTGTGGCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTGCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATaataaata                                                                                                           <  1:119377/251‑1 (MQ=255)
                                                                                                                                cgcgATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATAc                                                                                                          >  1:201863/1‑250 (MQ=255)
                                                                                                                                            aTCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAAtt                                                                                             >  2:190042/1‑251 (MQ=255)
                                                                                                                                                 gATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCAt                                                                                        <  1:168625/251‑1 (MQ=255)
                                                                                                                                                        cccGCGGGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAACGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCCACTTAAGCTCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATAACTCGTATTAAATTATCATGGCATa                                                                                  >  2:497228/1‑250 (MQ=255)
                                                                                                                                                         ccGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACACTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATaaa                                                                                <  1:747110/251‑1 (MQ=255)
                                                                                                                                                           gcgTGTGTCAGGACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCCTAAAGt                                                                              <  2:674367/251‑1 (MQ=255)
                                                                                                                                                                   cAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTcacc                                                                      >  2:381682/1‑251 (MQ=255)
                                                                                                                                                                      tACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTACGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCaccacc                                                                   >  2:537804/1‑251 (MQ=255)
                                                                                                                                                                       acgacgCTGCGCGAATGGCGAATAAAAAGCTGCGTGACGAGTTAATATTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGGGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCACCTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATCATAATTACATCGTATTAAATAATCATGGCAAAAAGAATTTCcccaccc                                                                  >  2:550691/1‑250 (MQ=255)
                                                                                                                                                                         gacgCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCaccacc                                                                   >  2:18352/1‑248 (MQ=255)
                                                                                                                                                                                  cgAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGac                                                        >  1:524242/1‑250 (MQ=255)
                                                                                                                                                                                                aaaGCTGCGTGCCGAGTTTATTTTCCAGCTCTTTAATATGAAAGCTGAGCGAAGGCTTAGACATAATTAAACTCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGAGCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACAGAAACACAAt                                         <  2:492304/251‑1 (MQ=255)
                                                                                                                                                                                                            cGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATAcc                             <  1:236050/251‑1 (MQ=255)
                                                                                                                                                                                                                  gATTTTCCAGCTCTTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTa                        >  1:580508/1‑250 (MQ=255)
                                                                                                                                                                                                                            ctctTTAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGt             >  1:111015/1‑251 (MQ=255)
                                                                                                                                                                                                                                 tAATATGAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAg         <  1:18352/250‑1 (MQ=255)
                                                                                                                                                                                                                                       gAATGCTGAGCGAAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAgg  >  1:197363/1‑251 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              
CCGCATAACCGGGCGCATCCGTCCCCACATTGCCGTTCCCACTACGCCGAGTTCAATCCGCCCTGCTTCTCCCCGACCTATTTGTT‑CAATCCGAGCCAATACATTATTAGCATTCACCAGCAATCGACGCGATTCTTCCATCAAGATTTTGCCCGCGTGTGTCAGTACGACGCTGCGCGAATGGCGAATAAAAAGCTGCGTGCCGAGTTGATTTTCCAGCTCTTTAATATGAATGCTGAGCGGAGGCTGAGACATATTTAAACGCGCTGCTGCGCGGCCAAAATGCAACTCTTCCGCTACGGCAAGAAAATAACGGAGCAACTTAAGATCTGTTCTGTATACGCGTTCCATAATTAAGTGCTCCATATAATAAATACATCGTATTAAATTATCATGGCATAAAGTATTTCACCACCAGATATCCGACATACATAAACACAATGGATAATATACCCGGTAATACATTTGGTACAGGAATAGG  >  NC_000913/2522127‑2522607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑