| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,867,311 | G→T | R81S (CGT→AGT) | rpoS ← | RNA polymerase sigma factor RpoS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,867,311 | 0 | G | T | 94.9% | 151.0 / ‑3.6 | 63 | R81S (CGT→AGT) | rpoS | RNA polymerase sigma factor RpoS |
| Reads supporting (aligned to +/- strand): ref base G (0/3); new base T (21/35); total (24/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
GGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACGCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTG > NC_000913/2867066‑2867557 | ggTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑CCC‑CAGCGCGCG‑ACtcgcc > 1:641209/1‑249 (MQ=255) ggTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATaa < 1:2549/251‑1 (MQ=255) ggTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATa < 1:226887/250‑1 (MQ=255) cATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAActt < 1:780957/251‑1 (MQ=255) aTCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAActtcttc < 1:393566/251‑1 (MQ=255) cccGTTCAATCGTCTGGCGAATCCACCAGGTTGCTTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑g < 2:605619/251‑1 (MQ=255) ttCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTAATCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGGGCGCG‑ACTCGCAAAAAAAACTTCTTCTTC‑GGCCg > 1:11731/1‑251 (MQ=255) aTCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTTACACCAGACGCAAGTTACTCTCGATCCTCCGGCGGCGGGAGGGGACATCTCC‑AAG‑CCGTTCGCG‑GCTCGCAAAAAAAACATCTTCTTC‑GGACGTTa > 1:340150/1‑250 (MQ=255) gtcGAATCCACCAGGTTGCGGATGTTGAGAAGCGGAAACCACTTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACTCCATACCACGATTTCCATAACGGCGGGCAATTTTTACCTCCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACCgtggt < 1:477941/249‑1 (MQ=255) gCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACGCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAgtggtg < 1:107141/251‑1 (MQ=255) aTCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATa < 2:430009/251‑1 (MQ=255) aacaccaAGTTGCGTATGTAGAGAAGCGGAAACCACGTTCCAGGACAAAAATCTCAACCGCGCGGAGCAGCCCCAGGTTGCCCTCTTCGAAAAGGTCCAGCAACGCCAGACCACGATAGCCATAACGGCGGGCAATTTTTACCACCAGACGAAAGTTACTCTCGATCAACCGGCGGCGAGAGGCGAAAAATCC‑ACG‑AAGTGCGCG‑AAACACAAAAAAAACTTCTTCAGC‑GGCAATTAACAGTGGTGAATaa < 2:400216/249‑1 (MQ=255) caggtgtcGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCACTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACTGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAAt < 2:198233/244‑1 (MQ=255) caGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCGCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAAt < 1:375936/251‑1 (MQ=255) aGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCCATTTTTTACACCCGACGCAAGTTAAACTCCGTCATCCCGCGGCGAGAGGCGACCTCTTC‑CCG‑CCATGCGGGCAC‑CGCAAAATAAAATTCTTTCTC‑GGCCGCTAACAGAGGAGCATAACCaaac > 1:145040/1‑249 (MQ=255) tGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGGCATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCCGCGGCGGGAGGCGACATCCCC‑CCG‑CAGGGCGCG‑ACTCGCCAAATAAAATTCTTCTTC‑TGCCGGTTACAGTTGTGAATAACCAAACTCAc > 1:9691/1‑251 (MQ=255) cGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCCAGTTACTCTTGATTATCCGCCGGCGGGAGGCGGCCTCTCC‑CCC‑CCGGGCGAG‑ACTCGCAAAATAAACATTTTCTTT‑GGCCGTTTACAGGGGTGAATAACCAATCTCACCACGGGAAAGCTGAgg > 1:334002/1‑250 (MQ=255) ggAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCCTCCGGCGGCGAGAGGCGACATCTCC‑CCC‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑TGCCGTTAACAGTGGTGAATAACCACTCTCACCAAGGTAAAACTGAGTc > 1:468990/1‑251 (MQ=255) ggAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGAACTTTTTACCACCAGACGCAAGGTACTCTCGCTCATCCGGCGGCGAGGGGCGACAACTCCCAC‑‑CAGTGAGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGAGAACAGTGGGGAATAAACAATCTCAcccaagaaaaactgaggc > 1:506131/1‑235 (MQ=255) gAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGAAGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑CGCCGTTAACAGTGGGGAATAACCAATCTCACCCAGGGAAAGCTGAGTcg > 1:721723/1‑251 (MQ=255) aaaCCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTTCCACCAACCGCCAGTTACTCTCTATCATCCCGCGGCGGGAGGCGCCCACTCC‑CCA‑CACTGCGCG‑CCACGCAAAAAAAACTTCTTCTTT‑GCGCGTTAAACGTGGGGAATAACCAAtactcccccaggaaaacggtgtgcc > 2:807675/1‑227 (MQ=255) cACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTTCCCTCTTCGATAAGGTCCAGCAACGCCAGTCCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTcc < 2:515034/251‑1 (MQ=255) cGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCGATTTTTTCCCCCCGACACCAGTTACTTTCGGTCATCCGGCGGCGGGAGGCGGCATCTCC‑CCC‑CAGTGCTCG‑ACTCCAAAAATAAACTTCTTCTTC‑TGCCGTTAACACTGGTGAGTAACCAATCTCACCaacagaaaactgagtagtgaccc > 2:402053/1‑229 (MQ=255) ccGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGGCCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacaca < 2:281113/251‑1 (MQ=255) cAAACTACTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGAGTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCCATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtgt < 2:1854/251‑1 (MQ=255) ccGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCCATTTTTATCACCCAACGCAAGTTACACTCGATCATCCGGCGGCGAGAGGCGCCATCTCC‑ACA‑CCCGGCCCG‑CCCCCCCAAATAAACATCTTTTTC‑GGCCGTTTACAGTGGGGAATGAACAATCTCACCCAGGCTAAGCTGTGTTGCGTCCacaccaccccatgtggctcccctc > 2:668101/1‑228 (MQ=255) cgGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAAATTTTAACACCCGAAACCAATTACTCTCGGACATCCGGCGGCGGGAGGGGACAGCTCC‑CCGGCCAT‑CGCG‑ACTCGCAAAAAAAACATCCTCTTC‑GGACGCTAAAAGGGGGGGAAAACAAATCTCAACAAGGGAAAGCTGAGGCGCGTCCAaaacacaccggggggccacccgcgata > 2:715506/1‑229 (MQ=255) tCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTAACCCCCGACGCAAGATACTCTCGATCATCCGGCGGCGAGAGGCGAACTCTCC‑ACC‑CCATGCGCG‑ACTCGCAAAAAAAACTTCTTCTTCTGCCCGTTA‑CAGTGGGGGATAAACAATCTCACCAAGGTAAAGCTTAGTCGCGTCCCACCCCCCCTGTGTTGCTCCCTCCGATAAccgt > 1:728041/1‑248 (MQ=255) ccTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCaaa < 1:48213/251‑1 (MQ=255) ctTCTATACGGTCCAGCCACTCCAGACCACGATTGCCACAACGGCCGTCAATTTTTACCAACAGACGCAAGTTACTCTCCATCATCCGGCGGCGAGAGGCGTCATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAATCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACTCGCTGTGTGGCACCCTGCGATAACAGTTCCTCTTCGTCCAAAt < 1:680874/249‑1 (MQ=255) ctTCGATAAGGTCCAGCAACGCCAGGCCACGATTGCCATAACGGCTGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCACAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGATTAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAAt < 1:12284/249‑1 (MQ=255) tCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGt < 1:703654/250‑1 (MQ=255) atcAACGCCAGACCACGATTGCCATAACGGCGGTCAATTTTTACCACCAGACGCAAGGTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑AAG‑CAGTGCGCG‑ACTCGCAAAATAACCTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTTTGGCGCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTc < 2:88510/249‑1 (MQ=255) cGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCCCCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGtt < 1:223841/251‑1 (MQ=255) gCCAGACCACGATGGCCACAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGAGCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTc < 2:721723/251‑1 (MQ=255) ccGATTGCCATAACGGCGGGCAATTTTTACCCCCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACT‑CAGTGCGCG‑ACGCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAgg < 2:83862/250‑1 (MQ=255) tGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACGCGCAAAATAAACTCCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCtt < 1:426667/250‑1 (MQ=255) gcaagaaCGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTAC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTGATCACTGGGTTCCTGTTCTACTAAGGCCtttt < 2:778820/246‑1 (MQ=255) gCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTc < 1:428022/251‑1 (MQ=255) ttACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCg > 1:317622/1‑251 (MQ=255) accaccAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGtt > 1:314060/1‑251 (MQ=255) accaccAGACGCAAGTTACCCGCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTTCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCCCCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGtt < 2:340150/251‑1 (MQ=255) aCGCAAGTTACGCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCaa < 1:310066/251‑1 (MQ=255) gCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAt > 1:290862/1‑251 (MQ=255) caatttACTCTCGACCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAtt < 2:668840/247‑1 (MQ=255) aGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTcc < 1:789590/251‑1 (MQ=255) gtgaCTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCg < 1:777972/248‑1 (MQ=255) aCTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTCTCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCAt > 2:715206/1‑251 (MQ=255) aCTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGGTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCAt < 1:487086/251‑1 (MQ=255) cgtccGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATc < 2:468990/247‑1 (MQ=255) tctcGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCt > 1:462619/1‑251 (MQ=255) cGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGGCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCtt < 1:402053/249‑1 (MQ=255) gATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGAt > 1:63354/1‑148 (MQ=255) gATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTCC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGAt < 2:63354/148‑1 (MQ=255) cggcggCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAACCCTCAACTCCGTTCTCATCAAATTCCGCCTCTTCCTTTAAATc > 2:312252/1‑251 (MQ=255) cggcgAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATg > 1:548595/1‑251 (MQ=255) agGCGACATCTCC‑ACG‑CCGTGCGCG‑ACTTGCAAAATAAACTTCTGCTAC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAGACCGCATCTTCATTTAAATCAGGAACTTTc < 2:145040/251‑1 (MQ=255) cGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGt > 1:638502/1‑164 (MQ=255) cGACATCTCC‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGt < 2:638502/164‑1 (MQ=255) cc‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTAt < 1:174595/247‑1 (MQ=255) cc‑ACG‑CAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCAGCTTCATTTAAATCATGAACTTTCAGCGTAt < 1:631694/247‑1 (MQ=255) cAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTg > 2:545248/1‑78 (MQ=255) cAGTGCGCG‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTg < 1:545248/78‑1 (MQ=255) cg‑ACTCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTg > 1:789454/1‑251 (MQ=255) | GGTTTGGTTCATAATCGCCCGTTCAATCGTCTGGCGAATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCC‑ACG‑CAGTGCGCG‑ACGCGCAAAATAAACTTCTTCTTC‑GGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTG > NC_000913/2867066‑2867557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |