| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,193,996 | T→G | D70A (GAT→GCT) | thiE ← | thiamine phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,193,996 | 0 | T | G | 99.1% | 393.4 / NA | 109 | D70A (GAT→GCT) | thiE | thiamine phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); major base G (61/46); minor base A (1/0); total (62/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
ATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCT > NC_000913/4193749‑4194240 | aTGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGCTCGCCAGCCGCCAGTAAGCGt > 1:358223/1‑251 (MQ=255) gcctgcccaggttttCCAGCCCCTGCGGGGCGGATGGCAGCTGTTGGGTGTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCGGGGAGACGTCTATTTCCATACCGTCATGTGTCGAACCGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATGGAGATCGGTGGCTTGCAAATCTCCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTGGATCGCAAGCCGCCAGTAAGCGtt < 2:464377/237‑1 (MQ=255) cGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGAGCGGATGGAATTGCGGTCGGTGGCTTGGAAATCTTCCTGCCCC‑AAAAGGGCGCCATACGCCTGCTGCTTGGTAGCCAGCCGCCCGCAAGCGTTGAt > 2:309118/1‑251 (MQ=255) cTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAAAAATc > 2:529265/1‑251 (MQ=255) tGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAgcg > 1:295616/1‑251 (MQ=255) gCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAgcg < 1:460964/250‑1 (MQ=255) aggcttttGTTTGGTTTGGCTCGGGACCACATGTCCCCGCGCGATATAAGGGGGGCGTGCTCCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAGCGCCCAGCCGCAGGCCGG‑CCGCGCGGATGGCATGGAGATCGGTGTCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATCCGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAgcg < 2:397964/244‑1 (MQ=255) cATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGCTAAACATTCGCGCGTTATAGCGG‑CGGACCCGCGCa > 2:781173/1‑250 (MQ=255) aTCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAAt > 1:173425/1‑251 (MQ=255) aTCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGAGTCAAAACGCCCAGCCGCAAGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAgcgacat > 1:452133/1‑247 (MQ=255) cTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTg > 1:623370/1‑251 (MQ=255) ggtttggtttgCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTgc < 1:767854/250‑1 (MQ=255) gtttggtttgCGTCGGGAACCCATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTgcc < 1:492769/251‑1 (MQ=255) gttgggtttgCGTCGGGAACACCTGTCCCAGATCGATAGAAGAGGGGCGTGCGGCCAGCGCGACGTCGATTTCCATAGCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGAGGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTgcc < 2:500441/247‑1 (MQ=255) gcttCGGGAACACTTGTCCCAGCGCGATATAAGAGGGGCGTGCTGCAAGCGCGACGTCAATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGAc < 1:296303/247‑1 (MQ=255) gTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATc < 1:539137/251‑1 (MQ=255) cGGGACCACATGTCCCAGCGCGATATAAGAGGGGCGTGCTCCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAGCGCCCAGCCGCGGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATCCGCCTGATGCTTGACCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGT‑CGGCCCAGCGCAATTGCCGCCACGACATCgg < 1:186608/251‑1 (MQ=255) gggAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGc > 1:529053/1‑251 (MQ=255) gggAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGc > 1:639284/1‑251 (MQ=255) tGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGGGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCCGCGCCAATGCCCCCACACCCACGGC‑TTCCAcccc > 2:493389/1‑249 (MQ=255) gccccAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACctc < 1:636724/248‑1 (MQ=255) ataAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGAGCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGACCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGAt < 1:281565/251‑1 (MQ=255) aagcggggCGTGCTGAAACCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGCTCGGTGGCTTGCAAATCTTCCTGCCCC‑ACATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGACCCATTGCCGACCCGCCCTAGCC‑TTCCAACTCTTCATCGCGCCgtcct > 2:495094/5‑247 (MQ=255) aaGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACCACATCAGC‑TTCCCCCCCTTCCTCGCCCCGGTCt > 2:543707/1‑251 (MQ=255) agGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCttt > 1:702080/1‑250 (MQ=255) gctgcCAGCGGGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTa < 1:691049/250‑1 (MQ=255) gctgcCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGt > 1:70864/1‑249 (MQ=255) gcCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGCCATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGCTGCGTAGCTg > 1:160628/1‑251 (MQ=255) cAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTTCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGAAGCTggg > 1:759267/1‑250 (MQ=255) aGCGCGACGACGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCACCAGCCGCCAGTCAGCGTTGATAAACAATCTCGCGTTATAACGGACGACCC‑GCGGACGTGCCGCCACGCCCTTGGG‑TACCACCTCCTCATCGGCCCGATCtttagtgccaatccag > 2:210908/1‑236 (MQ=255) gcgACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAgt < 1:84223/251‑1 (MQ=255) gACGTCGATTCCCATATCGTCATTTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAgtac < 1:115868/251‑1 (MQ=255) gcGTCTATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAgtacg < 1:404516/250‑1 (MQ=255) tgATTTCCATAACGTGATGTGTCGAACCGCCCAGCCGCAGGCACG‑CCGCGCGGAGGGCATTGAGACCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAgtacgtac < 1:359051/249‑1 (MQ=255) tatCGTCATGTGACGAAACGCCCCGCCGCAGGCCTG‑CCGCGCGGATGACATTGATATCGGTGGCTTGCAAATCTTCCTGCCCC‑AGATGGACACCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAACGTTGATAAACCATCGCGCGTTATCGCGG‑CGGCACAGCGCAATTGACGCCACGACCACGACGTTCCAC‑TCGTCACCGACCCGATCTTTGATGAGTCGCAGG‑GGAGTACGGAAGCCCGCATcc > 2:61141/1‑251 (MQ=255) atCGTCATGTGCCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATcc < 1:574311/250‑1 (MQ=255) gggcggaaccccccAGCCGCAG‑CCTGTCCGGGCGGAGGGCATGGAGATGGGTGGCTTGAAACTCTCCCGCCCCC‑AAATGGACGCCATACGCCTGAGGCTGGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACg < 2:554185/239‑1 (MQ=255) gtCGAACCGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGGGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTc < 1:519383/251‑1 (MQ=255) cGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCg < 1:529265/250‑1 (MQ=255) ccccAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑t < 1:297581/250‑1 (MQ=255) ccAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCCGCGCAATTGCCGCCACGACCTCGGC‑TTCCACCTCTTCATCGCTCCGATCTTTGATGCGTTGCTGG‑AAAGTCCGAACGCCTTCATCCAACAGACGATCGATCCaa > 2:671535/1‑247 (MQ=255) gcccccAGGCCTG‑CCCCCCGGATGCCATTGAGAGCGGTGGCTGGCAA‑TCCTCCCGCCCCCAAATGGACGCCTAACGCCTGATTCTGGTCCCCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTAc < 2:174132/246‑1 (MQ=255) aGCCGCAGGCATG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑AGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGAGCTCTGATGCGTAGATGG‑AGAGAACGGACCCCTGCATCCAACAGACGTTCGATCCAC‑TGTa > 1:492817/1‑250 (MQ=255) gCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTAAGCCTGCATCCAACAGACGTTCGATCCAC‑TGTCCGCTg > 1:550397/1‑251 (MQ=255) cAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGAACTTTATTTCGTAGCTTG‑AGAGTACGGCCCCCCGCATCCCACAGACGTTCGATCCAC‑Tggaccctgg > 2:68094/1‑243 (MQ=255) aGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATCCGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGGCATCGGC‑CTCCCCCTCTTCCTCCGGCCGCTCTTTGCTGCGTAGCTGG‑GGTGTACGTACGCCTGCATCCACCAGCCTGCCGATCCCC‑TGTACGCCGT‑c > 2:114797/1‑251 (MQ=255) aGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCATCAGACGTTCGATCCAC‑TGTACGCTGT‑c > 2:540295/1‑251 (MQ=255) aGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑c < 1:19213/251‑1 (MQ=255) ggCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑cc > 1:816592/1‑251 (MQ=255) tG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCCTCGCGCCGATCTTTGATGCGTAGCTGG‑GGAGGACGTACGCCTGCATCCAAAAGACGTTCGGTCCAC‑TGTAAGCTGT‑ccaccc > 2:228712/1‑250 (MQ=255) tG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGGAGCTGGCAG‑GTACGTACGCCTGCCTCCAACAGACGTTCGATCCAC‑TGGACGCtagccacc > 2:641937/1‑243 (MQ=255) cgGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGt > 1:154611/1‑250 (MQ=255) ggATGGCATTGAGATCGGTGGCTTGCAACTCTTCCTGCCCC‑AACTGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCACCGAAATAGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGGTCTGTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCAACCACCAAACGTTCGATACCC‑TGTAAGCAGA‑CCACCACCCGGTAc > 2:307078/1‑251 (MQ=255) ggATGGCATTGAGATCGGTGGCTTGCAAATCTTACTGACCA‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCCGTAAGCGTTGATAAACACTCGCGCGTTATAGCGG‑CGGCCCAGCGCCATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGCTCTTTGATGCTTAGCTGG‑AGAGTACGTACGCCCGCATCCCACAGACGTTCGATCCCC‑TGTACGCTGTACCAC‑ACCGGGTAc > 2:802250/1‑251 (MQ=255) aTGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCCTCGCGCCGGTCTTTGCTGCGTAGCTGG‑AGAGTACGTACGCCTGCCTCCAAAAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACac > 2:354426/1‑250 (MQ=255) ggCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGAAGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGAAATTGCCCCCACGACATCGGC‑TTCCACCTCTTCATAGCGCCGATCTTTGATGCGTAGCTGG‑AGGGTACGTACCACTGAATCCCCAAGAAGTTAGTTCCCA‑TGTACGCTGT‑CCACCACCGGGtcaagtc > 2:482073/1‑245 (MQ=255) cATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCt > 2:253550/1‑251 (MQ=255) tGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGtt > 1:722525/1‑100 (MQ=255) tGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGa > 1:443687/1‑250 (MQ=255) tGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTGGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGtt < 2:722525/100‑1 (MQ=255) gatatggggggCTGGCAAACCTCCCCGCCCC‑AAATGGACGCCATACGCCTGTGGCTTTACCCCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAAc < 2:39282/242‑1 (MQ=255) gagaTCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGCTCCAC‑TGTACGCTGT‑CCACACCCGGGAACCGTCCTGAAc > 2:321428/1‑251 (MQ=255) aaTCGATGGCTTGCAACTCATCCTGCCCC‑AACTGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATCACCACTCGCGCGTTATAACGG‑CGGCCCACCGCAATTGCCGCCACGCCCTCGGC‑TTCCACCAATTCATCACGCCGATCTTTTATGCGTATCTTG‑AGAGTACGTACGCCTGCATCCAACAGCCGTTCAATC‑ACATGTACGACGT‑CCACCACCGGGTACAGGCCTGAAcc > 2:788915/2‑249 (MQ=255) ccGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGaaa < 1:253550/250‑1 (MQ=255) cGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGaaa > 1:13180/1‑250 (MQ=255) cGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGACGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTTCCGGCCCGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGTTCCAA‑TGTACGCTGT‑CCACCACCGGGtccgatcctgcaagaaa > 2:495457/1‑234 (MQ=255) gTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAACCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCAAGACATCCGC‑TTCCACCTCTTCCTCGGCCCGATCTTTTATGCGTAGCTGG‑GGAGTACGTTCGCCCTCATCCAACAGACGTTCGAATCAC‑TGGACCCCTT‑CCACCACCCGGGACAGTccctaaagaaa > 2:214697/1‑239 (MQ=255) ggCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCt < 1:318585/154‑1 (MQ=255) ggCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCt > 2:318585/1‑154 (MQ=255) gcgtgaaaatttcccTGCCCA‑AATTGGCCGCCATCCGCTGGATGTTTGGCCGCCAGCCGACAGTAAGCGTGGACAAACAATCGCGCGTTATAGCGG‑CGGCCAAGTGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTGGATGCGTAGCTGG‑AGAGTACGTACGCCTGCAGCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCCCCGGGTACAGGCCTGAACGAAAAGGTAc < 2:358615/238‑1 (MQ=255) tGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCAAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGAGTACAGTCCTGAACGAAAAGGTACagg > 2:555728/1‑251 (MQ=255) gCAAATCTCCCTCCCCC‑AAAGGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACagg < 1:627957/250‑1 (MQ=255) ccTTCCTGCCCC‑AAAGGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGaaa < 1:556140/250‑1 (MQ=255) gCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGc > 1:40539/1‑251 (MQ=255) c‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGAt > 1:493952/1‑251 (MQ=255) aaTGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATAc > 1:362830/1‑251 (MQ=255) aaTGGACGCCATACGCCTGATGCTTGACCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATAc < 1:811171/251‑1 (MQ=255) gACGCCATACGCCGGATGCTTGACCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCa < 1:321428/251‑1 (MQ=255) cGCCATACGCCTGATGTTTGATCTCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACg < 1:404711/251‑1 (MQ=255) ccATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCTCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCAGCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCt < 1:257784/251‑1 (MQ=255) ccATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAACGTAAAGGAGGAAAATCAGGCTgctaactcccgct > 2:795786/1‑239 (MQ=255) cgtgcgcctaatgcctggccGCCATCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCTCCTCTTCAGCGCGCCTATCGTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCtt < 1:364387/232‑1 (MQ=255) cATACGCCTGATGCTTGACCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCtt < 1:563187/251‑1 (MQ=255) aTACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCa > 1:122552/1‑174 (MQ=255) aTACGCCGGATGCTTGACCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCa < 2:122552/174‑1 (MQ=255) ccTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCGGCCTCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCTTGAACGACAAGAGACAGGAGGAAAAGCAGGCTGAGAACTCACGcctcctcct > 2:227653/1‑251 (MQ=255) tGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGGTCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCtt > 1:604836/1‑250 (MQ=255) gATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAACCAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTGCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTAc < 1:31870/251‑1 (MQ=255) atctttgtgcgcaccccgccAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCAGGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCTTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACAGCACGCTTCCTCCTTAc < 2:816592/235‑1 (MQ=255) tGATCCCCCGCCGCCAGTAAGCGATGATAAACAATCGCGCGTTAGAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTGCATCGCGCCGATCTTGGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAg < 1:309118/249‑1 (MQ=255) gCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGAtt > 1:165086/1‑251 (MQ=255) gCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGAGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGGACAGTCATGAACGAAAAGGAAAAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGAtt > 2:670936/1‑251 (MQ=255) ccAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGAttt > 2:299515/1‑251 (MQ=255) cAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTATGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTtc > 1:390197/1‑251 (MQ=255) ggccgccAGTAAGCGTTGATAAACAACCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCTCGCCATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTtct < 2:529053/250‑1 (MQ=255) aGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCCTCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTtct > 2:379345/1‑251 (MQ=255) gccgccAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTtctc > 1:228280/1‑251 (MQ=255) cgccAGTAAGCGTGGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCg < 1:141202/251‑1 (MQ=255) ccAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACCTCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGcc > 1:331245/1‑251 (MQ=255) cAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGG‑CGTCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCct > 1:300768/1‑251 (MQ=255) | ATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTG‑CCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCC‑AAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGG‑CGGCCCAGCGCAATTGCCGCCACGACATCGGC‑TTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGG‑AGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAC‑TGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCCTTACGCAGGTAGATTTCTCCGCCT > NC_000913/4193749‑4194240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |