BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,730,560	C→T	W69* (TGG→TAG)	xylA ←	xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,730,560	0	C	T	99.0%	347.2 / NA	103	W69* (TGG→TAG)	xylA	xylose isomerase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (53/49); minor base G (0/1); total (53/50)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 103 total aligned reads displayed.

GCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTG  >  NC_000913/3730313‑3730798

gcCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACg                                                                                                                                                                                                                                             >  1:21070/1‑251 (MQ=255)

   gTAGCGGGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGAc                                                                                                                                                                                                                                          >  2:108721/1‑251 (MQ=255)

     aGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACAGCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACg                                                                                                                                                                                                                                         <  2:21070/250‑1 (MQ=255)

     aGCGAGGGTTTGTAAAGCAGTTGGCCGTCCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATGTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGTAAAACTCAAATGCGGCATCTGCTGTCCGCTTCGCCAACGCCAGTGCCTCGCCAGGCTGCTGCTACGGActa                                                                                                                                                                                                                                        <  2:48407/251‑3 (MQ=255)

       cgcgggTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGAtt                                                                                                                                                                                                                                      >  1:208779/4‑251 (MQ=255)

       cGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGAtt                                                                                                                                                                                                                                      <  1:604543/251‑1 (MQ=255)

           ggTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAAc                                                                                                                                                                                                                                  <  1:21425/251‑1 (MQ=255)

            gTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACg                                                                                                                                                                                                                                 <  1:680560/251‑1 (MQ=255)

              ttGTAAAGCAGTTGGCCGTTCCCCACGGCAGCTTCACGCCCCTCTCTTCTTGCTTGCCTGCCAGGACATCAAACATTTGAGCAAAATTATTGATGT‑ACTATTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGcc                                                                                                                                                                                                                               >  1:325567/1‑251 (MQ=255)

                  aaaGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAACATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGccccc                                                                                                                                                                                                                            >  2:414585/1‑250 (MQ=255)

                   aaGCAGTGGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCcac                                                                                                                                                                                                                          <  1:319608/251‑1 (MQ=255)

                       aGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACAccc                                                                                                                                                                                                                      >  2:387392/1‑250 (MQ=255)

                       aGTTGGCCGTTCACCACAGCAGCTTCACGCCGCTCTCTCCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACAcc                                                                                                                                                                                                                       >  2:382213/1‑250 (MQ=255)

                          tGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAAc                                                                                                                                                                                                                   >  1:543915/1‑251 (MQ=255)

                                ttCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACCTGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGGTTAAACGCCCCCACACCAAACATATcc                                                                                                                                                                                                             >  2:58254/1‑251 (MQ=255)

                                 tCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATcc                                                                                                                                                                                                             <  1:814068/250‑1 (MQ=255)

                                        agcagcTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGtt                                                                                                                                                                                                     <  1:817614/251‑1 (MQ=255)

                                          cagcTCCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACAGCAACCATGTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAAAGCGACATCGGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTcc                                                                                                                                                                                                   <  2:766794/251‑1 (MQ=255)

                                               tCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCcagca                                                                                                                                                                                               <  1:486527/250‑1 (MQ=255)

                                                   gccgcTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCTCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCAACACCAAACAAATCCGCCCCGTTCCAGAAGAAgg                                                                                                                                                                                          >  2:809377/1‑251 (MQ=255)

                                                         ctcttcgtgttggccggccAGGACATCAACCATTTGTGCAAAATTATTGATTT‑ATTCTTTTAACGACGCGCCCTCAGTGGAAACATCCACATCGTTGAAGCAATAAAAGTGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCGTACCAGGCTGCTGCTACGCACGATTAAACGCGCCCACACCAAACATATCCGCCCCGATCCAGCAGAAGGtgtg                                                                                                                                                                                      <  2:785235/233‑1 (MQ=255)

                                                           cttcttGCTGGCCTGACAGGACATCAACCATGTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTGAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATTCGACATCTGCCTTACGCTTCGCCAACGCCAGTGGCTCACCAGGCTGCTGCGACGAACGATTAAACGCCCCCTCACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGc                                                                                                                                                                                     <  2:464545/248‑1 (MQ=255)

                                                             tcttGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAg                                                                                                                                                                                  <  1:659310/249‑1 (MQ=255)

                                                                tgcttgcCTGCCAGGACAGCAACCATGTGCGCAAAATTATTGATGT‑ACTCGTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAg                                                                                                                                                                               <  1:711341/249‑1 (MQ=255)

                                                                         gccAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCa                                                                                                                                                                      <  1:693539/249‑1 (MQ=255)

                                                                                 aTCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGt                                                                                                                                                            >  1:338747/1‑251 (MQ=255)

                                                                                   cAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCGCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGc                                                                                                                                                          <  2:55130/251‑1 (MQ=255)

                                                                                     aCCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAACCGCCATTGctc                                                                                                                                                        >  2:260206/1‑251 (MQ=255)

                                                                                     aCCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGctc                                                                                                                                                        >  1:263368/1‑251 (MQ=255)

                                                                                        aTTTGCGTAAAATTATGGATGTTAC‑ATTTTAACGACGCGCCCTCAGGGGAAACATCCACCTCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACGCAAAGGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCGCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTc                                                                                                                                                     <  2:274663/251‑1 (MQ=255)

                                                                                                aaaaTTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCCTACGCt                                                                                                                                             >  2:625684/1‑251 (MQ=255)

                                                                                                     tattGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGACCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGAGGCAAAACGCAAGTGCTCTTCCATCCGCTTAccc                                                                                                                                        >  2:499139/1‑251 (MQ=255)

                                                                                                     gattGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGAAACTTGTGGAAAAACTCAAATGCGAAATCTGCTTTACGCTTCGCCAACGCCAGGGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGAAGCAGGCGGCAAAACGCAAGTGCACTTCCATAAGCTTAccc                                                                                                                                        >  2:643161/2‑251 (MQ=255)

                                                                                                                ctctTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGtt                                                                                                                              >  1:99436/1‑251 (MQ=255)

                                                                                                                 cctgttAACGCCCCGCCCTCAGGGGAACCATCCACAACGTTGAAGCAATTAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCCCACCAGTCTGCTGCTACGGACGATTAAACGCCCCCACCCCAAACATACCCGCCCCGTTCCAGCAGAAGGTGTTCCAGTAGCAGGCGGCAAAACTCAAGTGCTCTTCCATACGCTTACCCAACACCAGTtc                                                                                                                             <  2:381751/247‑1 (MQ=255)

                                                                                                                    tttAACGACGCGCCCTCAGGGGAAACATCCACCTCGTCGAAGCCATACACTGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGAACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATCCGCTTACCCAACACCAGTtcgtc                                                                                                                          >  1:282613/1‑251 (MQ=255)

                                                                                                                      tAACGACGCGCCATCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAATATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAATGCTCTTCCATACGCTTACCCAACACCAGTTCGTCgg                                                                                                                        >  1:584900/1‑251 (MQ=255)

                                                                                                                          gacgCGCCCTCAGGGGAAACATCCACATCGTGGACGCAATAAAATGGCACATGTAACTTGTGGAAACACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCACACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCCGGCGGCAAACCGCAAGCGCTCTTCCATACGCTTACCCAACACCAGTTCGTCgggctt                                                                                                                    >  2:548158/1‑248 (MQ=255)

                                                                                                                          gacgCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACCTGTAACATGTGGAAACACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATCTCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCCAAACGCAAGTGCTCTTCCATACACTTACCCCCCACCAGTTCGTCggggtt                                                                                                                    >  2:556541/1‑248 (MQ=255)

                                                                                                                              cgcCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGt                                                                                                                  >  2:426812/1‑249 (MQ=255)

                                                                                                                               gcCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACCTGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGt                                                                                                               >  1:209255/1‑251 (MQ=255)

                                                                                                                                 ccTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGa                                                                                                             <  1:241915/251‑1 (MQ=255)

                                                                                                                                        ggAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATg                                                                                                      <  1:143512/251‑1 (MQ=255)

                                                                                                                                            acaaccACATCGTGGAAGCAATAAAAGGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCGTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTaa                                                                                                  <  2:77361/247‑1 (MQ=255)

                                                                                                                                             cATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAAc                                                                                                 <  1:707229/251‑1 (MQ=255)

                                                                                                                                             cATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGGAGTGACGGAATGCTAAc                                                                                                 <  2:351609/251‑1 (MQ=255)

                                                                                                                                             aaacctcaTCGTGGAAGCAATAAAATGGCCCATGTTACTTGTGGAAAAACTCAAATGCGGCATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGTATTGTAGTGACGGAATGCTAAc                                                                                                 <  2:80806/245‑1 (MQ=255)

                                                                                                                                              aTCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACg                                                                                                <  1:197412/251‑1 (MQ=255)

                                                                                                                                              aTCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACg                                                                                                >  2:493627/1‑251 (MQ=255)

                                                                                                                                              aTCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACg                                                                                                <  1:710640/251‑1 (MQ=255)

                                                                                                                                                ccACATCGTTGAAGCAATAAAAGGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACggg                                                                                              <  2:700807/251‑1 (MQ=255)

                                                                                                                                                     acGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCGGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGa                                                                                         <  2:445550/250‑1 (MQ=255)

                                                                                                                                                             gCAATAAAATGCCACATGTATCTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGTGTTTGAGGATTTTg                                                                                 <  2:144670/251‑1 (MQ=255)

                                                                                                                                                               aaTAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTg                                                                                 >  1:616194/1‑249 (MQ=255)

                                                                                                                                                               aaTAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTg                                                                                 >  1:415879/1‑249 (MQ=255)

                                                                                                                                                                aTAAAATGGCCCATGTACCTTGCTTAAAAACTCAAATGCTACATCTGCTGTACTCTCCGCCTAGGCCAGGGGCTCTCCAGTCTGCTGCTGCGGACGATTAAACGCCCCCACACCAAACAGATCCGCCCCTTTCCAGCATAATGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGTGCTTCCATACGCTTACCCAAGTCCAGTTCGTCTGGATTGTAGTGACGGAATGCTGACGGGTTTGAGGATTTTGAGc                                                                              <  2:357540/251‑1 (MQ=255)

                                                                                                                                                                  aaaaTGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGcc                                                                             <  1:643161/250‑1 (MQ=255)

                                                                                                                                                                       ggCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATa                                                                       <  1:375748/251‑1 (MQ=255)

                                                                                                                                                                        gCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATa                                                                       >  1:686773/1‑250 (MQ=255)

                                                                                                                                                                         cacaTGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaac                                                                     >  1:182399/1‑251 (MQ=255)

                                                                                                                                                                             tGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCCAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCACAACGCAAGGGCTCTTCCATACGCTTACCCACCACCAGTTCGTCGGGCTTGTAGTGCCGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacgaac                                                                 >  2:350707/1‑251 (MQ=255)

                                                                                                                                                                              gTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacgaacg                                                                <  1:387392/251‑1 (MQ=255)

                                                                                                                                                                               tAACTTGTGGCACAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATaacgaactc                                                               >  2:76086/1‑249 (MQ=255)

                                                                                                                                                                                aaCTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAAcgc                                                               <  1:228728/250‑1 (MQ=255)

                                                                                                                                                                                aaCTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAAcgc                                                               <  1:458217/250‑1 (MQ=255)

                                                                                                                                                                                    tgtgGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATc                                                           <  1:815244/250‑1 (MQ=255)

                                                                                                                                                                                    tgtgGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATc                                                           <  1:260206/250‑1 (MQ=255)

                                                                                                                                                                                     gtgGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCg                                                          >  1:42791/1‑250 (MQ=255)

                                                                                                                                                                                         aaaaaCTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTg                                                     >  1:751952/1‑251 (MQ=255)

                                                                                                                                                                                         aaaaaCTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTg                                                     <  1:468874/251‑1 (MQ=255)

                                                                                                                                                                                          aaaaCTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTgg                                                    >  1:140657/1‑251 (MQ=255)

                                                                                                                                                                                              cTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCa                                                 >  1:587222/1‑250 (MQ=255)

                                                                                                                                                                                              cTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCa                                                 >  1:172572/1‑250 (MQ=255)

                                                                                                                                                                                               tCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAggcgg                                                                                                                                                                        <  1:210303/130‑1 (MQ=255)

                                                                                                                                                                                               tCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAggcgg                                                                                                                                                                        >  2:210303/1‑130 (MQ=255)

                                                                                                                                                                                                taaaTGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCaa                                                <  1:70078/248‑1 (MQ=255)

                                                                                                                                                                                                cAAATGCGTCATCGGCTTTTCGCTTCGCCAACGCCAGTGCCTCGCCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATAGCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCaaa                                               <  2:692845/250‑1 (MQ=255)

                                                                                                                                                                                                cAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCaaa                                               >  1:224451/1‑250 (MQ=255)

                                                                                                                                                                                                 aaaTGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAAt                                             >  1:797150/1‑251 (MQ=255)

                                                                                                                                                                                                   ccttccgaccTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAg                                           <  2:596234/242‑1 (MQ=255)

                                                                                                                                                                                                   aTGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAg                                           <  1:167670/251‑1 (MQ=255)

                                                                                                                                                                                                             ttcTTTACGCTTCGCCCACGCTAGTGCCTCCCCCGGCTGCTGCTCCGGACGCTTAAACGCCCCCACACCCAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGCAGATTTTGAGCCTTCATAACACACGCGATCGATCTGGTCAAAATAGGCTTGCatat                                 >  1:254462/3‑251 (MQ=255)

                                                                                                                                                                                                              gCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAg                                           >  1:596234/1‑240 (MQ=255)

                                                                                                                                                                                                               cTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTg                               >  1:713856/1‑251 (MQ=255)

                                                                                                                                                                                                                tttACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAATGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTg                               >  1:143238/1‑250 (MQ=255)

                                                                                                                                                                                                                       ttCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGCGCCAGTAGCAGGCGGCAAAACGCAAGTGATCTTCCCTACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGACTGCTAACGGGTTTGAGGATTTTGAGCCTTCTTAACGAACCCGATCGAGCTGGTCAAAATAGGCTTGCATCTTGAACTGCAt                       >  2:22151/1‑251 (MQ=255)

                                                                                                                                                                                                                         ccccAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTGCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATa                      <  2:713856/248‑1 (MQ=255)

                                                                                                                                                                                                                         cGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAAGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATa                      >  1:470071/1‑250 (MQ=255)

                                                                                                                                                                                                                                gCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGGAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGTGATGGGCACAATAGGCTTGCATTTTGAATTCCATAATCAGGt               >  2:252828/1‑250 (MQ=255)

                                                                                                                                                                                                                                 ccAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTGTCCAACACCAGTTCGTCGGGATTGTAGTTACGGAATGTTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAGCGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTaa             <  1:762882/251‑1 (MQ=255)

                                                                                                                                                                                                                                   aGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATg           >  1:685511/1‑251 (MQ=255)

                                                                                                                                                                                                                                    gTGCCTCCCCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGc          <  1:592995/251‑1 (MQ=255)

                                                                                                                                                                                                                                     tGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGACCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACg                                                                                                                                               <  1:381280/117‑1 (MQ=255)

                                                                                                                                                                                                                                     tGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGACCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACg                                                                                                                                               >  2:381280/1‑117 (MQ=255)

                                                                                                                                                                                                                                       ccTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCCCACAAAACATATCCGCCCCGGTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCcgc       <  2:152591/251‑1 (MQ=255)

                                                                                                                                                                                                                                       ccTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAACCATATCCGCCCCGTTCCAGCAGAAGGCGTGCCAGTAGCAGGCGGCACAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATTGGATTGCATATTGAACTCCATAATCCGGTAATGCcgg       >  2:511674/1‑250 (MQ=255)

                                                                                                                                                                                                                                          cACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAACCATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCCTAATCAGGTATTGCCGCggg    >  2:507173/1‑251 (MQ=255)

                                                                                                                                                                                                                                           aCCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCCGGCGGCAAAACGCAAGTGCTCTACCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCgggg   >  2:662817/1‑250 (MQ=255)

                                                                                                                                                                                                                                           aCCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCgggg   >  1:465937/1‑250 (MQ=255)

                                                                                                                                                                                                                                            ccAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCAAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTg  <  1:108721/251‑1 (MQ=255)

GCCGTAGCGAGGGTTTGTAAAGCAGTTGGCCGTTCCCCACAGCAGCTTCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGT‑ACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTG  >  NC_000913/3730313‑3730798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`