Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,555,047 G→C V655L (GTC→CTC)  malT → DNA‑binding transcriptional activator MalT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,555,0470GC96.6% 285.3 / 3.4 89V655L (GTC→CTC) malTDNA‑binding transcriptional activator MalT
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base C (37/48);  total (39/50)
Fisher's exact test for biased strand distribution p-value = 5.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.17e-01

TGCGCATTCGTGCGCAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTTGCTGCTTAATCAA  >  NC_000913/3554802‑3555292
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
tGCGCATTCGTGCGCAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCgg                                                                                                                                                                                                                                                  >  2:287657/1‑251 (MQ=255)
             gcAGCTGTTAGGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGc                                                                                                                                                                                                                                     <  2:658211/251‑1 (MQ=255)
              ccgCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTTGCGGGATTGAAGTCTTGGCGGCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCTAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCa                                                                                                                                                                                                                                    <  1:739069/249‑1 (MQ=255)
              cAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCa                                                                                                                                                                                                                                    <  1:742474/251‑1 (MQ=255)
                gctttggtGGGCCTGGGCGCGGCTGGATGAAGCCGCAGCGTCGGCGCGTCGCGGGATTGAAGTCTTGTCGCCTTACCAGACACAGCAACCGCTTCAGAGCCTGGCAATGTTGATTCACTGCTCGACAGCCCGTGTGGCTTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTAGGACATGGCAAAGATCACCGCGCCTGGATACCTAACGCCAACAAGCTCCGATTGATTTACGGGCaa                                                                                                                                                                                                                                   >  2:269619/8‑250 (MQ=255)
                  tGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATg                                                                                                                                                                                                                                >  1:71192/1‑251 (MQ=255)
                        gggCCTGGGCGGGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTGCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGTATCTCTAACGCCACCAAACTCCGGGTGATTTACTGGCACATGACCGGc                                                                                                                                                                                                                          <  2:247525/251‑1 (MQ=255)
                         ggctggggCGCGGCTGGAGGAAGCTGAAGCTTCTTCGCGTGGCTGGGTTCAAGGCTTGTCGGCTTATTGGTCACAGCAACATGTTCAGGTCCTGTCAATGTTTACTCAATGCTCGCTGGCCCTTGCTGATTTAGAAAACGCCCGGATCCATCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCTCAGTGACAGGATCTCTAACTCCAACAATCTCCGGGTGATTTACTGTCAAATGACCGGCg                                                                                                                                                                                                                         <  2:815754/246‑1 (MQ=255)
                         ggCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCg                                                                                                                                                                                                                         <  2:196273/251‑1 (MQ=255)
                         ggCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGGAGCGGGATTGAAGTCGTGGCGTCTTATCAGCCACAGCAACAGCGTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCg                                                                                                                                                                                                                         <  2:395574/251‑1 (MQ=255)
                             ggggCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATaa                                                                                                                                                                                                                     <  1:208179/250‑1 (MQ=255)
                              gggCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGGAGCGGGAATGAAGTCTTGTCGGCTTAACAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATaaa                                                                                                                                                                                                                    <  1:270904/251‑1 (MQ=255)
                                   cgGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAgccgc                                                                                                                                                                                                               <  1:268507/251‑1 (MQ=255)
                                        ggATGAAGCCGAGGCGTCGGCGCGTAGCGGGATTGAAGCCTTGTCGGCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGAGTCAATGCTCGCTGGCCCTTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTg                                                                                                                                                                                                          <  2:681950/251‑1 (MQ=255)
                                         gATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGc                                                                                                                                                                                                         >  1:744006/1‑251 (MQ=255)
                                               gCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAATCCTGCTGGGGAATGGCAAATATCACAGCTACTGGATCTCTAACGCCAACAACCGCCGGGTGATTTACGGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTg                                                                                                                                                                                                   <  1:486181/251‑1 (MQ=255)
                                                   aaGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTg                                                                                                                                                                                               <  1:315343/251‑1 (MQ=255)
                                                           gcgcGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATAc                                                                                                                                                                                       <  1:179943/251‑1 (MQ=255)
                                                                    gggATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAAcc                                                                                                                                                                              >  2:663632/1‑251 (MQ=255)
                                                                            aGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGCTTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCCGAGTTTg                                                                                                                                                                      >  2:336090/1‑251 (MQ=255)
                                                                                   ccgtcTAATCAGCCACAGCAACAGCTCCATTGCCTGGCAATGGTGATGCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGACCCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGaacaa                                                                                                                                                               <  2:146029/250‑1 (MQ=255)
                                                                                    cgtcTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGaacaac                                                                                                                                                              <  1:719350/251‑1 (MQ=255)
                                                                                     gtcTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGAGTCATACGGCTAAACCAGAGTTTGCGAACAAcc                                                                                                                                                             >  2:670258/1‑251 (MQ=255)
                                                                                      tctatccatccccggCAACAGCTTCAGTGCCTTGCAATGGTTATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGACCCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCCCTAACGCCAACAACCTCCGGGCGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAAcc                                                                                                                                                             <  2:33578/236‑1 (MQ=255)
                                                                                       cTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGATGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCAc                                                                                                                                                           >  1:335899/1‑251 (MQ=255)
                                                                                        ttATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACt                                                                                                                                                          >  1:346150/1‑251 (MQ=255)
                                                                                         tATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAACCCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCa                                                                                                                                                            <  1:734428/248‑1 (MQ=255)
                                                                                         tATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCAc                                                                                                                                                           <  2:312955/249‑1 (MQ=255)
                                                                                          aTCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACtt                                                                                                                                                         <  2:664883/250‑1 (MQ=255)
                                                                                             cgccccaGCAACAGCTTCAGTGCCTGGCAATGTGGATTCAAGGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACCTCCTg                                                                                                                                                     <  2:526638/246‑1 (MQ=255)
                                                                                                 acaGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAg                                                                                                                                                 <  1:663632/251‑1 (MQ=255)
                                                                                                 acaGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCGGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCGAAACCAGAGTTTGCGAACAACCACTTCCTGCAAg                                                                                                                                                 <  1:221441/251‑1 (MQ=255)
                                                                                                  caGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAgg                                                                                                                                                <  1:269619/251‑1 (MQ=255)
                                                                                                    gCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTc                                                                                                                                              <  1:77532/251‑1 (MQ=255)
                                                                                                      aaCAGCTTCAGTGCCTGGCAAGGTTGATTCAATGCTCGCTGGCCCGTG‑TGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGTTCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAAt                                                                                                                                           <  1:737352/251‑1 (MQ=255)
                                                                                                         aGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCGGCAAGGTCCATgg                                                                                                                                         >  2:604924/1‑251 (MQ=255)
                                                                                                            ttCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCAt                                                                                                                                                                                         <  2:242100/200‑1 (MQ=255)
                                                                                                            ttCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCAt                                                                                                                                                                                         >  1:242100/1‑200 (MQ=255)
                                                                                                              cAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGCCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCa                                                                                                                                     >  2:78104/1‑250 (MQ=255)
                                                                                                                  gCCTGGCAATGTGTATTCAAGCCTGGCTGGCCCGTGGTGAGTTAGATAACGCCCGTAGCTAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGTCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACAtt                                                                                                                                <  2:90073/251‑1 (MQ=255)
                                                                                                                       ccAATGTTGATTAAAGGCCCGCTGGCCCGGGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCg                                                                                                                           <  2:19489/250‑1 (MQ=255)
                                                                                                                        cAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCg                                                                                                                           >  1:19489/1‑250 (MQ=255)
                                                                                                                         aaTGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTg                                                                                                                         <  1:297939/251‑1 (MQ=255)
                                                                                                                            gTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACCCCCGTAGCCAGCTGTACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACGTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGca                                                                                                                       <  2:811165/250‑1 (MQ=255)
                                                                                                                              tGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGACCGTGcaca                                                                                                                     >  2:34545/1‑250 (MQ=255)
                                                                                                                                     aTGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCg                                                                                                                                                                                                                                                   <  2:219861/117‑1 (MQ=255)
                                                                                                                                     aTGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCg                                                                                                                                                                                                                                                   >  1:219861/1‑117 (MQ=255)
                                                                                                                                        cTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTgg                                                                                                          >  2:342179/1‑251 (MQ=255)
                                                                                                                                           gCTGGCCCGTGGTGATTTATATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCAAACTGGTTGCGTCATACGGATAAACCAGAGATTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCg                                                                                                       >  2:668749/1‑251 (MQ=255)
                                                                                                                                           gCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGGGCACAAATATTGCTGGGCg                                                                                                       >  2:386504/1‑251 (MQ=255)
                                                                                                                                           gCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCg                                                                                                       >  2:605337/1‑251 (MQ=255)
                                                                                                                                           gCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCg                                                                                                       >  2:441479/1‑251 (MQ=255)
                                                                                                                                            cTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCg                                                                                                       >  2:649896/1‑250 (MQ=255)
                                                                                                                                             tGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCg                                                                                                       >  1:118564/1‑249 (MQ=255)
                                                                                                                                              ggCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCGGCGGGGGAAGGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGTGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTGCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGt                                                                                                    <  2:275194/251‑1 (MQ=255)
                                                                                                                                                 ccGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGACCAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTg                                                                                                 >  1:330906/1‑251 (MQ=255)
                                                                                                                                                    tggtgATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTTCACAAATCTTGCTGGGCGGGTTTGAAc                                                                                              >  2:132668/1‑251 (MQ=255)
                                                                                                                                                     ggtgATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTAAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAAcc                                                                                             <  1:670258/251‑1 (MQ=255)
                                                                                                                                                     ggtgATTTAGATAACGCCCGTAGCAAGCTGAACCGGCTGGAAAACCGGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGGGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAAcc                                                                                             <  2:303455/251‑1 (MQ=255)
                                                                                                                                                      gtgATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCg                                                                                            >  1:374781/1‑251 (MQ=255)
                                                                                                                                                            tAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGa                                                                                       >  1:311969/1‑250 (MQ=255)
                                                                                                                                                              gATAAAGCCCGTGGCCAGCTGAACCGGCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGAGCTCTAACGCCAACAACCTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAAt                                                                                    <  2:450341/251‑1 (MQ=255)
                                                                                                                                                                        gTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGCGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCga                                                                           >  2:238349/1‑250 (MQ=255)
                                                                                                                                                                         tAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGCGTTTGAACCGGCAGAAATTGTTCTCga                                                                           >  2:226946/1‑249 (MQ=255)
                                                                                                                                                                          aGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCCAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAACTCTTGCTGGGCGAGTTTGAACCGGCAGCAATTGTTCTCgtagc                                                                        >  1:334062/1‑247 (MQ=255)
                                                                                                                                                                          aGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCgaaga                                                                        <  2:554341/251‑1 (MQ=255)
                                                                                                                                                                               gCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCACCTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTc                                                                    >  1:276532/1‑250 (MQ=255)
                                                                                                                                                                                 tGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAAt                                                                 >  1:703394/1‑251 (MQ=255)
                                                                                                                                                                                  gAACCTTCTGGAAAACCGGCTGGGGAAGGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACGGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGTCAGAAATTGTTCTCGAAGAACTCAAt                                                                 <  1:605711/250‑1 (MQ=255)
                                                                                                                                                                                      cGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGaaaa                                                            <  1:287657/251‑1 (MQ=255)
                                                                                                                                                                                             aaaaCCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCgg                                                     >  1:587621/1‑251 (MQ=255)
                                                                                                                                                                                                 ccTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTc                                                 <  1:437242/251‑1 (MQ=255)
                                                                                                                                                                                                       gtgtaaggcctataacaacaGCGACTGGAACTCTAACGCCACCAAACGCCGGGTGATTTCCGGGAAAATGACCGGCGAGAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGTGCGAGTTTGAACCTGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGTAGTCTGCGGt                                           <  2:357865/234‑1 (MQ=255)
                                                                                                                                                                                                       ggggAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCgg                                            <  1:274246/250‑1 (MQ=255)
                                                                                                                                                                                                           aaTGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTtgat                                       >  1:154334/1‑251 (MQ=255)
                                                                                                                                                                                                              ggCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAACCCGGGTGATTTACTGGCAAATGACCGGCGATAAATCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCACGTTCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGATTCTGCGGTtgatga                                     <  2:29616/250‑1 (MQ=255)
                                                                                                                                                                                                                 aaaTACCACAGGGACTGGATCTCTAACGCCAACAAACCCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTGCGCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGa                                 <  2:118564/251‑1 (MQ=255)
                                                                                                                                                                                                                  aaTATCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGAt                                <  1:240976/251‑1 (MQ=255)
                                                                                                                                                                                                                      tCACAGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGAtctc                             >  2:513826/1‑250 (MQ=255)
                                                                                                                                                                                                                      acacaggggctggatcccaagagCCAACAATCTCCGGGAGATTGACTGGCAAATGACCTGGGATAAAGCCGCCGCTGCCAACTGGGTTCGGCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCa                            <  2:652151/229‑1 (MQ=255)
                                                                                                                                                                                                                         caGCGACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAAcc                         >  1:474849/1‑251 (MQ=255)
                                                                                                                                                                                                                             gACTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAAATCAATGAAAATGCCCGGAGGCTGCGGTTGATGTGCGATCTCAACCGTa                      >  2:259927/1‑250 (MQ=255)
                                                                                                                                                                                                                              aCTGGATCTCTAACGCCAACAAACTCCGGGTGATTTGCTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGTTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGaa                                                                                               <  1:67445/176‑1 (MQ=255)
                                                                                                                                                                                                                              aCTGGATCTCTAACGCCAACAAACTCCGGGTGATTTATTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAAACAGAGTTTGCGAACAACCACATCCTGCAAGGACAATGGCGCAACATTGCCCGTGCCCAAATCTTGCTGGGCGAGTTTGaa                                                                                               >  2:67445/1‑176 (MQ=255)
                                                                                                                                                                                                                               cTGGATCTCTAACGCCAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAAcc                   >  1:68103/1‑251 (MQ=255)
                                                                                                                                                                                                                                       cgaaCGCCAACAAACTCCGGGTGATTTACTGGCAATTGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTtgctg           <  2:629302/249‑1 (MQ=255)
                                                                                                                                                                                                                                          aCGCCAAGAAACTCCGGGTGATTTTCGGGCAAATGCCCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTTGCTGCtt        <  2:634720/251‑1 (MQ=255)
                                                                                                                                                                                                                                             ccAACAAACTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTTGCTGCTTAAt     <  1:551522/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 caaACTCCTGGTGATTTACTGGCAAATGACCGGCGCTAAATCCGCCCCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGACATTTTTCTCGAAGAACTCACTGAAAATGCCCGCAGTCTGCGGTTGATGAGCGCTATCAACCTCAACCTGTTGCTGCTTAATcca  >  2:266556/1‑248 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
TGCGCATTCGTGCGCAGCTGTTATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTGATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTTGCTGCTTAATCAA  >  NC_000913/3554802‑3555292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑