BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,936,855	T→G	A424A (GCT→GCG)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,936,855	0	T	G	99.1%	363.6 / NA	110	A424A (GCT→GCG)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (57/52); total (57/53)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 110 total aligned reads displayed.

ACGGCGTGGCAATGCTAATGGGTCACCAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCTGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCTGGTTTGC  >  NC_000913/2936608‑2937097

aCGGGGTGGCTATGCTAAGGGGTCACCAGCTCTCCGTCACCGCTAGGGGATTTGCCTATTGGCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGTCATAAACGGGATGGACTGGCAGAACACGGAATCCTCCATTGGATCACCTCCGGTTCTGCTGCGCTGGTCGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCTCACTGGGAAATC‑TCTCAGTAAGAGGCGGA‑c                                                                                                                                                                                                                                                  <  2:647163/251‑1 (MQ=255)

  ggCGTGGCAATGCTAATGGGTCACCAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGc                                                                                                                                                                                                                                                >  1:396360/1‑251 (MQ=255)

    cGTGGCAATGCTAATGGGTCACCAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCtt                                                                                                                                                                                                                                              >  2:21919/1‑251 (MQ=255)

          aaTGCTAATGGGTCACCAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTcg                                                                                                                                                                                                                                        >  1:427356/1‑251 (MQ=255)

          aaTGCTAAATGGTCACCAGCTCCCCGGAACCGCTCAGGGAGTGGCCGATTGGCGGTCCTACTGGTCACCAGAA‑GCAAGTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTcg                                                                                                                                                                                                                                        <  2:59861/251‑1 (MQ=255)

               tAATGGGTCACCAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCt                                                                                                                                                                                                                                   >  1:704577/1‑251 (MQ=255)

                     gtccccAGCTCCCCGGTACCGCGCAGGTATTTGCGGTTGTGCGTCCCAACTGGTCAC‑AGAAAGCAATTGGGCGTGTAACGGGGCATAAACTGGATGGACGGGGAGACCAGGGCATCACCCATTGGATCACCTCCGGTTCTGCTGCGCTGGCCGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGaa                                                                                                                                                                                                                             <  2:128262/247‑1 (MQ=255)

                     gTCACCAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGa                                                                                                                                                                                                                              >  1:272238/1‑250 (MQ=255)

                          cAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTg                                                                                                                                                                                                                        >  2:189043/1‑251 (MQ=255)

                            gCTCCCCGGCACCGCTCAGTTATTTGCCGTTGTGCGTCCCTCCTGGTCACCAGAA‑GCAATTGAGCGTGTACCGGGGCATAAACTTGATGGACGGGCAGAACACGGCATCATCCTTTTGATCAACCCCGGTTCTGCGGCGCTGGACGGTTCCTTTAAACAACGCGTCCGCGAAGGTA‑CCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGccc                                                                                                                                                                                                                     <  1:746084/251‑1 (MQ=255)

                             cccaccggacccGCTCAGGTAGTTGCGGATGTGCGTACCGACTGGCCACCAGAA‑GCAATTGGGGGTGTAACGGGTCATAAACGGGATGGACTGGCAGAACACGGCCTAACCCTTTTGATCAACTCCGTTTCTGCGGCGCTGGCCGGTTCCTGTAAACAACGCGCCAGCGAAGGTACCCCGACG‑ATGAAGCCACACGGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGccc                                                                                                                                                                                                                     <  2:397487/241‑1 (MQ=255)

                              ccACCTGCACCGCTCAGGTATTGGCCGATGTGCGTCCCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTGCGGCTGCGCTGGACGGTCCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCCCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCg                                                                                                                                                                                                                    <  1:194124/250‑1 (MQ=255)

                                   ggCACCGCTCAGGTATTTGCCGCTGTGCGTACCTACTGGTCACAAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGGTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAACCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGACTGGTGCCCGGCGa                                                                                                                                                                                                                >  2:305151/1‑250 (MQ=255)

                                    gccccGCCCAGGTATTGGCCGATTGGCGTCCCTACTGGTCACAGGAA‑GCAATTGGGGGGGTAACGGGGCATAAACGGGATGGACTGGAAGAACCCGGCACCATCAATTTGACCAACCCCGGTTCTGCTGCGCTGGACGGTTCCTGTAACCAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGGGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATc                                                                                                                                                                                                              <  2:212801/248‑1 (MQ=255)

                                       ccGCTCAGGTATTTGCCGCTGTGCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCAc                                                                                                                                                                                                           >  2:666365/1‑251 (MQ=255)

                                       ccGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGCA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCCCCGCTACCGACTGGTGCCCGGCGGTCCAc                                                                                                                                                                                                           >  2:781403/1‑251 (MQ=255)

                                       ccGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCgtacccc                                                                                                                                                                                                           >  2:333205/1‑245 (MQ=255)

                                            cAGGTATTTGCCGATGTGCGTCCCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATa                                                                                                                                                                                                      <  1:693477/251‑1 (MQ=255)

                                            cAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAA‑GCCATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTACTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGGAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGC‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAAt                                                                                                                                                                                                       >  1:127485/1‑250 (MQ=255)

                                              gttATTTGCCGATGTGCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACt                                                                                                                                                                                                    <  1:729037/249‑1 (MQ=255)

                                                  tttGCCGATGTGCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTcc                                                                                                                                                                                                 <  1:146478/250‑1 (MQ=255)

                                                           gtgCGTACCTCCGGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAAACCCCGGAATAACCCATTGGATCACCCCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCTTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGgcggcg                                                                                                                                                                                        <  1:791563/250‑1 (MQ=255)

                                                             gCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGtt                                                                                                                                                                                     <  1:556633/251‑1 (MQ=255)

                                                             gCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGtt                                                                                                                                                                                     >  1:485808/1‑251 (MQ=255)

                                                              cgttcctgcTGGTCACCAGAA‑GCAATTGAGCATGTAACGGGGAATAAACTGGATGGACGGGCAGAACACGGCATCATCCATTTGACCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAACCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTAGCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTa                                                                                                                                                                                    <  1:633724/243‑1 (MQ=255)

                                                              cGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGtt                                                                                                                                                                                     >  1:305468/1‑250 (MQ=255)

                                                                 aCCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTActc                                                                                                                                                                                 >  1:320720/1‑251 (MQ=255)

                                                                   cTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGCTCCACGAATCCTTCCGTGGCGGCGGTTACTCtt                                                                                                                                                                               >  2:233572/1‑251 (MQ=255)

                                                                   cTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCCCGAATACTCCCGTGGCCGCGGtttcccct                                                                                                                                                                               >  2:227800/1‑245 (MQ=255)

                                                                       tGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCg                                                                                                                                                                           >  1:298282/1‑251 (MQ=255)

                                                                       gggTCACCAGAA‑GAAAGTGAGCGTGGAACGGGGCATAAACGGGATGGACTGGCAGAACACGGCATAATCCATTTTAGCAACTCCGGTTCTGCGGCGCTGGACGTTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCg                                                                                                                                                                           <  2:706257/250‑1 (MQ=255)

                                                                            ccccagaggaaaTTGAGCGTGTAACGGGCCATAACCGGGATGGACGGCCAGATCACGGCATCATCCATTTGATCACCCCCGGTCCTGCGCCCCTGTACGGTCCCTGTAAACAGCGCGACAGCGAAGGTACCCCGACA‑ATGAAGCCACCCTGGAAAATC‑TCACAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTc                                                                                                                                                                       <  1:305151/241‑1 (MQ=255)

                                                                               aGAA‑GCAATGGAGCTGGTAACGGGGCAAAAACTGGAGGGACTGCCAAAACACGGCCTCATCCATTTGTTCAACTCGGGTTCTGCGGCGATGTACGGTTCCTGAAACCAACGCGACACCGAAGGTACCCCTACA‑ATGAAGCCACACTGGGAAATC‑CCTCAGCAAGAGGCGGA‑CGCTTCCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTa                                                                                                                                                                   <  2:718229/251‑1 (MQ=255)

                                                                                 aa‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTACCCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTAcc                                                                                                                                                                 <  1:189043/251‑1 (MQ=255)

                                                                                   aggaattgcgggggtaacgggtccaaaaCTGGAGGGACTGGGAGAACGCGGCACCATCCTGTTGATCATCGCCGGTTGTGCTGCGCTGTGCGTTCCCTGGAAACAACGCGACAGCGAAAAAAACCCGACG‑TAGACGCCACACTGGAAAACC‑TCTAGGCAAGAG‑CGGAGCTCGTGCCTCGCCGCGACCGAATGGTGCCCGGCGATGCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCg                                                                                                                                                                <  2:103973/226‑1 (MQ=255)

                                                                                     cAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGa                                                                                                                                                               >  1:524858/1‑250 (MQ=255)

                                                                                      aaTTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTc                                                                                                                                                                                                                                         >  1:208690/1‑175 (MQ=255)

                                                                                      aaTTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGGGGCGGCGGTTACTCCTCCCGCCTCCtttcccaa                                                                                                                                                              >  2:398636/1‑244 (MQ=255)

                                                                                      aaTTGAGCGTGTAACGGGGCATAAACCGGATGGACGGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTc                                                                                                                                                                                                                                         <  2:208690/175‑1 (MQ=255)

                                                                                      aaTTGAGCGGGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACCCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAg                                                                                                                                                             <  1:571860/251‑1 (MQ=255)

                                                                                         tGAGCGTGTACCGGGGCATAAACTGGCTGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑CTGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTAATTTTCCCGGTTCCtttcccgcag                                                                                                                                                            >  2:129049/1‑241 (MQ=255)

                                                                                           ggCGTGTAACGGGGCATAAACTGGATGGACTGGCAGACCTCGGCATCATCCATTGGATCACCTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAggcggc                                                                                                                                                        <  1:588829/250‑1 (MQ=255)

                                                                                           aGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGATACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACCCTTCCCGCCTCCtttccccaagcggc                                                                                                                                                        >  2:744081/1‑239 (MQ=255)

                                                                                           aGCGTGGAAAGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATGTGAGCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTATCCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGTTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAggcggc                                                                                                                                                        <  1:44611/251‑1 (MQ=255)

                                                                                             ggtgtAACGGGGCATAAACGGGATGGACTGGCAAAACACGCCATAATCCATTTGATCAACCCCGGTTCTGCGGCGCGGGAGGGTTCCTTAAAACAACGCGACAGCGAAGGTACCCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCTGA‑CGCTTGCCTCGCCGCTACCGAGTGGTGCCCGGCGATCCACGAATACTTCCGTTGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGt                                                                                                                                                      <  2:248858/250‑1 (MQ=255)

                                                                                               tgtAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCACCTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGCATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGCAGGCGGCGTcc                                                                                                                                                    >  1:544672/1‑251 (MQ=255)

                                                                                                 tAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGGTACTCTTCCCGCTTCCTTACCCAAgg                                                                                                                                                            >  2:574906/1‑241 (MQ=255)

                                                                                                 tAACGGGGCATAAACGGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACCCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAgg                                                                                                                                                            <  1:574906/241‑1 (MQ=255)

                                                                                                    cGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTc                                                                                                                                               <  1:666115/251‑1 (MQ=255)

                                                                                                              aCTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTc                                                                                                                                     <  1:631965/251‑1 (MQ=255)

                                                                                                                         tGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTTCCGAAGGCGGCGTCCCGTTCCCCATGACCCGTGTCaccctc                                                                                                                          >  2:259616/1‑246 (MQ=255)

                                                                                                                          ggCAGAACACGGCATCATCCATTTGAACAACCCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAAcagca                                                                                                                         <  1:674906/251‑4 (MQ=255)

                                                                                                                             aGAACACGGCATCCTCCATTTGATCAACCCCGGTTTGGCCGCGGTGGACTGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTTCCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACAtcatca                                                                                                                      <  1:636535/251‑1 (MQ=255)

                                                                                                                             aGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCCTCCCCGTCCCCCTGACTCGTGTCAACatcatc                                                                                                                       >  2:750717/1‑250 (MQ=255)

                                                                                                                                acacGGCATCATCCTTTTGATCACCTCCGGTTCTGCTGCGCTGGACGGTCCCTGTAAACACCGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAg                                                                                                                   <  1:333205/251‑1 (MQ=255)

                                                                                                                                  acGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGGTCACCATGGCTCGTGCCAAACTCCTCAAAGGc                                                                                                                 >  1:515897/1‑251 (MQ=255)

                                                                                                                                   cGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCCAAGGcc                                                                                                                >  1:7455/1‑251 (MQ=255)

                                                                                                                                        ccacccATGTGATAAACCCCGGTTCTGCTGCGGTGGACGGTTCCTGTAAACAACGCGACAGCAAAGGAAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCCCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGa                                                                                                           <  2:396360/247‑1 (MQ=255)

                                                                                                                                             cATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTAATCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTATCCACCACATCAAAGGACTGGGCCCggg                                                                                                      >  2:490337/1‑250 (MQ=255)

                                                                                                                                                  gATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCCTCACGGAGGCGGCGTCCCGTTCCCCATGAATCGTGTCAACATCATCCAAGGCCTGGGACCGGTACTGc                                                                                                 >  2:40970/1‑251 (MQ=255)

                                                                                                                                                     cAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTa                                                                                                                                                                   >  2:36034/1‑182 (MQ=255)

                                                                                                                                                     cAACTCCGGTTCTGCTGCGCGGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCaa                                                                                               <  1:627685/250‑1 (MQ=255)

                                                                                                                                                     cAACCCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTa                                                                                                                                                                   <  1:36034/182‑1 (MQ=255)

                                                                                                                                                           cGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCCGCGTCCCGTTCCCCATGACTCGTGTCCACATCCTCCAAGGCCTGGGCCCGGGACTGCCAATCGCgg                                                                                        >  2:167381/1‑251 (MQ=255)

                                                                                                                                                            gtttCTGCTGCGCGGGACTGTCCCTGTAAAAACCGCCACAGCGAAGGTACCCCGACG‑AGGAAGCCACACGGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGGCCCGTTCACCATGACTCGAGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGa                                                                                       <  2:524858/249‑1 (MQ=255)

                                                                                                                                                               cctgcggCGCTGGACGGTTCCTGTAAACACCGCGAAAGCGAAGGAAACCGGACG‑AGGAAGCCCCCCTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAg                                                                                     <  2:333108/249‑1 (MQ=255)

                                                                                                                                                                 tgctgcGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAAGCGGCGTCCCGTTCACCAAGAATCCTGTGCACATCCTCCAAGGCCCGGGACCAGGACTGCacagcgccaaaggtt                                                                                  >  2:607810/1‑237 (MQ=255)

                                                                                                                                                                    tgcGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTggg                                                                               >  1:37305/1‑250 (MQ=255)

                                                                                                                                                                      cgcTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGGTCACCATGACACGTGTCAACATCCTCAAAAGCCTAGGCCCGGTACCGCAAATCGCGGaaagcctgagg                                                                             >  2:323389/1‑242 (MQ=255)

                                                                                                                                                                           gACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAAGCGGCGTCCCGTTCAACATGACTCGTGTCAACATCATCCAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTgg                                                                        >  1:760086/1‑250 (MQ=255)

                                                                                                                                                                             cGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGGCTGGGACCGGTACTGCAAATCGCGGAAGGCCGGA‑GCGGGGAAt                                                                     >  2:299238/1‑251 (MQ=255)

                                                                                                                                                                             cGGTTCCTGAAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAAt                                                                     <  1:21919/251‑1 (MQ=255)

                                                                                                                                                                              ggtcccTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAAtt                                                                    <  1:107749/247‑1 (MQ=255)

                                                                                                                                                                                 tCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGc                                                                  <  1:40970/250‑1 (MQ=255)

                                                                                                                                                                                  ccTGTAACCAACGCGACAGCGAAGGTAACCCGCCG‑ATGAATCCACCCGGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTTCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCg                                                                <  1:666365/251‑1 (MQ=255)

                                                                                                                                                                                  ccTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCg                                                                >  1:499244/1‑251 (MQ=255)

                                                                                                                                                                                  ccTGTAAAAACCGCAACAGCGAAGGACACCCGAAG‑ATGACGCCACCCGGGGAAATC‑TCCCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCg                                                                <  2:211923/251‑1 (MQ=255)

                                                                                                                                                                                   cTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGttgtttacag                                                                 >  1:134913/1‑240 (MQ=255)

                                                                                                                                                                                        aaaacaccacaagcaaaGGTACCCCGACG‑ATGAAGCCACACTGGGAAATC‑TCCCAGCAAGGGGGGGC‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGAt                                                          <  2:579680/236‑1 (MQ=255)

                                                                                                                                                                                           accgcgACAGCAAAGGAACCCCGACG‑ATAAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGC‑CGCTTCCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGAtgtg                                                       <  2:789848/249‑1 (MQ=255)

                                                                                                                                                                                                 aCAGCGAAGGTAACCCGACG‑ATGACGCCACACTGGGAACTC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCCTGACTCGTGTCAACATTATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGGGGAATTGCCGAAGGATGGGCATGAc                                                 >  1:252145/1‑251 (MQ=255)

                                                                                                                                                                                                  cAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGCAGGCGGCGTCCCGTTCACCCTGCCCCGTGGCAAAATCCTCCAAGGCCTGGGCCCGGTACTGCAAAACGCGGAAGGCTGGA‑GCGTGGAATTGCCCAAGGATGTGCAttacc                                                >  2:467779/1‑247 (MQ=255)

                                                                                                                                                                                                   aGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGATGTGCATGACAt                                               >  1:685403/1‑251 (MQ=255)

                                                                                                                                                                                                   aGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGATGTGCATGACAt                                               >  1:429355/1‑251 (MQ=255)

                                                                                                                                                                                                   aGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGATGTGCATGACAt                                               >  1:413465/1‑251 (MQ=255)

                                                                                                                                                                                                   aGCGAAGG‑AACCCCACGGATGACGCCACACGGGGAAATC‑GCTCAGCAAGAGGCGGA‑CGCTGGCCGCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGATGTGCATGACAt                                               <  2:536218/251‑1 (MQ=255)

                                                                                                                                                                                                    gtggaggggaccccgacattGAACCCACA‑TGGGAAACCCTCTCAGCAAGGGGCGGA‑CGTTTGCCTCGCCGCTACCGAATGGTGCCCGGCGTTCCACGAATACTTCCGTGGCGGCGGTTACGCTTCCCGCTTCCTTACCGAAGGCGGCGGCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGATGTGCATGACAt                                               <  2:502798/232‑1 (MQ=255)

                                                                                                                                                                                                    gCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGATGTGCATGACATc                                              <  1:40502/251‑1 (MQ=255)

                                                                                                                                                                                                     cGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAAGCGGCGTCCCGTTCACCCTGACTCGTGTCACCATCATCAAAGGCCTGGGGCCGGGACTGGCACGCGCGGAAGGCTGGA‑GCGAGGAATTGCCGCAGGATAGACATGACATcc                                             >  2:619438/1‑251 (MQ=255)

                                                                                                                                                                                                     cGAAGGTAACCCGACG‑ATGAAGCCACACGGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCgtg                                                                                                                                  <  1:591355/167‑1 (MQ=255)

                                                                                                                                                                                                       aaGGTAACCCGACG‑ATGAAGCCACACTGGGCAATC‑TATCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCAACATGACTCGTGTCCACATCCTCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGGGGAATTGCCGCAGGCTGTGCATGACctcctc                                           >  1:655223/1‑251 (MQ=255)

                                                                                                                                                                                                         ggTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGATGTGCATGACATCCTcaa                                         <  1:103510/251‑1 (MQ=255)

                                                                                                                                                                                                         ggTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTGCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGATGTGCATGACATCCTcaa                                         <  1:227800/251‑1 (MQ=255)

                                                                                                                                                                                                          gTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGCAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGCTGTGCATGAAATCCTcaac                                        >  1:687110/1‑251 (MQ=255)

                                                                                                                                                                                                                  acg‑aTGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCAc                                >  1:478486/1‑251 (MQ=255)

                                                                                                                                                                                                                      aTGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCa                              >  1:447933/1‑250 (MQ=255)

                                                                                                                                                                                                                                   gggAAATC‑TCACACAAAGCGGCGGA‑CGCTTCCCTCGCCGCTACCGAATGGTGCCCGGCGCTCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTGCCGTTCACAATGACACGTGTCACCATCATCAAAGGCCTGGCACCCGTACTGCAAATCGCGGAAGGCAGGA‑GCGTGGAATTTCCGCAGGATGTGCATGGCATCTTCAAAAAACCCACCAAATCAACCTGGcc                 >  2:754813/1‑250 (MQ=255)

                                                                                                                                                                                                                                     gAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAATGCTGGA‑GCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCCACTCAACCTGGCCAac              >  1:519870/1‑251 (MQ=255)

                                                                                                                                                                                                                                     gAAATC‑TCTCAGCAAGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCAGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTACCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGCAGGATGTGCATGACCTCCTCAACAAACACACCAACTCAACCTAGCCAac              >  1:366934/1‑251 (MQ=255)

                                                                                                                                                                                                                                                   aaGAGGCGGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTCCTCTTGCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCCTGCCTCGTGCCAACATCCACAAAAGCCTGGGAACGGGACAGCCAATCGCGGAAGGCTGGAAGC‑TGGAATTCCCGACGGGTGTGACCGACACTCTCAACCAACGCACCCAATCAACCTGGCCACCCACCCGGTTTGc  >  2:700982/1‑250 (MQ=255)

ACGGCGTGGCAATGCTAATGGGTCACCAGCTCACCGGCACCGCTCAGGTATTTGCCGATGTGCGTACCTACTGGTCACCAGAA‑GCAATTGAGCGTGTAACGGGGCATAAACTGGATGGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGTTCCTGTAAACAACGCGACAGCGAAGGTAACCCGACG‑ATGAAGCCACACTGGGAAATC‑TCTCAGCAAGAGGCTGA‑CGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGA‑GCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCAACCACCTGGTTTGC  >  NC_000913/2936608‑2937097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`