Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,307,733	C→T	A295T (GCG→ACG)	yojI ←	ABC transporter family protein/microcin J25 efflux protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,307,733	0	C	T	86.5%	226.0 / 0.7	91	A295T (GCG→ACG)	yojI	ABC transporter family protein/microcin J25 efflux protein
Reads supporting (aligned to +/- strand):  ref base C (1/11);  new base T (50/27);  total (51/40)
Fisher's exact test for biased strand distribution p-value = 2.70e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGCCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTGCGCATCAGGAATGTAGAGGTTG  >  NC_000913/2307486‑2307983                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                             cTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCAc                                                                                                                                                                                                                                                           >  1:601889/1‑251 (MQ=255)   ttgtggtcgATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGGCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCAcc                                                                                                                                                                                                                                                          <  2:777807/242‑1 (MQ=255)   ttGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCt                                                                                                                                                                                                                                                         >  1:167048/1‑251 (MQ=255)            aTACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTc                                                                                                                                                                                                                                                 >  2:313517/1‑250 (MQ=255)              aCAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCCCCTGCGCCGTcagc                                                                                                                                                                                                                                              >  2:60215/1‑251 (MQ=255)                aaGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTcagc                                                                                                                                                                                                                                              >  1:205699/1‑249 (MQ=255)                  gCCCGTCAACAACAGCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCg                                                                                                                                                                                                                                          <  2:270760/251‑1 (MQ=255)                      gTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGACGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCAGCCCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAACCGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAAGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCCGCGCCGTCAGCAGCGT‑Cgg                                                                                                                                                                                                                                     >  1:601694/1‑251 (MQ=255)                         aacaacATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCa                                                                                                                                                                                                                                   >  1:138842/1‑250 (MQ=255)                             acaTCGCCAGCGTCTATTATCCGCTCCCGTGGCCGCCAACCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGTAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATgcg                                                                                                                                                                                                                              <  2:228536/251‑1 (MQ=255)                                  gCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑c                                                                                                                                                                                                                        >  1:310678/1‑251 (MQ=255)                                    gaGCGGCGAATTTCCGCTACCGTTGCCGCAACTCAGAAACAGCAGCTCGCAAAGTTTGATGGGGAGATTAATAGGACAAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCCCCAG‑CGTTT‑GCCAGTTGGGAAAAGACTGCGGGCGAGGAACCTCTG‑CTTAGAAAGGCGCGAGCGCGAATTT‑GTTCAGATTGTTAA‑ACGTCACATGCGACGGCAGCAGCTT‑CGACAATACGCCAA‑ccg                                                                                                                                                                                                                      >  2:704593/2‑251 (MQ=255)                                           gATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAACGT‑CGGCAATGCGCCAA‑CCGCCGaa                                                                                                                                                                                                                 >  2:541024/1‑249 (MQ=255)                                                cccGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAgcg                                                                                                                                                                                                          <  1:280646/250‑1 (MQ=255)                                                   gCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAgcggcg                                                                                                                                                                                                       >  1:186493/1‑251 (MQ=255)                                                      aCCGTTGCCGCCACTCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTAc                                                                                                                                                                                                    >  1:574481/1‑251 (MQ=255)                                                      aCCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCCATGCGCCAA‑CCGCCGAAAGCAGCGGCGTAc                                                                                                                                                                                                    >  1:15832/1‑251 (MQ=255)                                                           tGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCg                                                                                                                                                                                                                                                   >  1:727931/1‑201 (MQ=255)                                                           tGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTGACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCg                                                                                                                                                                                                                                                   <  2:727931/201‑1 (MQ=255)                                                              cgccAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAAt                                                                                                                                                                                            >  1:479080/1‑251 (MQ=255)                                                               gccAAACAAAAACAGCAGCCCGCCACTTTTGATGGTGAAATAAATCGGACCAACGGAAAACGCGTTACCTTGATAAGAAAACGTCCCGGTCCGCAGCTCCAG‑CGTTG‑GCCAGTTGGGAAACGCCGGCGGGCGCGGAAACTCTG‑TTTGGAAAGGCGCGAGCGCGATTTG‑GTTCAGCTTGTTAA‑ACGTCCCCTGCGCCGCCAGCAGCGT‑CGGAAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATa                                                                                                                                                                                           <  2:244254/251‑1 (MQ=255)                                                                  aaccaaaaacagaatcgcGCCACGTTTGTTGGATAGATTAACCGGCCCAACGGAAAACGCGTTATCCTGATAAGAAAACGTCACGTTCCACAGCTCAAG‑CGTTT‑GCCATTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑TTTTGAAAGGCGCGAGCGCGAATGT‑GTTCAGCTTTTTAA‑ACGTCACCGGCGCCGTCAGCACCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGTAATaaaa                                                                                                                                                                                        <  1:740614/234‑1 (MQ=255)                                                                      aGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAACCTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGt                                                                                                                                                                                    >  2:474434/1‑251 (MQ=255)                                                                               ggcTCGACACGTTTGATGGTGAGATTAATCGGACCAAAGGAAAACGCGTTATCCTGCTAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGCAAGCAGCGGCGACCGCAGGAATAAAAACGTCAACGAAt                                                                                                                                                                            >  2:511310/2‑250 (MQ=255)                                                                                gcTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAAt                                                                                                                                                                            >  1:765999/1‑249 (MQ=255)                                                                                gcTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAAt                                                                                                                                                                            >  1:387575/1‑249 (MQ=255)                                                                                gatCGCCACGTTTGAGGGGGGAATAAATCGGACCACCGAAAACCGCGTTATCCTGATAAGCAAACGTCACGTGACGCAGCTCCAG‑CGTTG‑GCAAGTTGGGAAACGCCTGCGGGCGCGAAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑TTTCAGCTTGTTAA‑ACGTCCCCGGCGCCGCCAGCAGCGG‑CGGCAATGCCCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAg                                                                                                                                                                          <  2:315920/249‑1 (MQ=255)                                                                                 cTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAgg                                                                                                                                                                         >  1:33192/1‑251 (MQ=255)                                                                                   cGCCACTTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTCCGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGAAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAgg                                                                                                                                                                         <  1:784521/249‑1 (MQ=255)                                                                                   cGCCACGTTTGATGGTGAGATTAATCGGACCAACGAAAAACGCGTTATCCTGATAAGAAAACGTCACGTGACGCAGCCCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑TTCCGGCTTGTTAA‑ACGTCACCGGCGCCGTCAGCAGCGG‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAATCGTCAACGAATAGGTc                                                                                                                                                                       <  2:171988/251‑1 (MQ=255)                                                                                     ccACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCAAGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGACTTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAAGAATAAAAGCGTGAACGCATAGGTcgc                                                                                                                                                                     >  2:541687/1‑251 (MQ=255)                                                                                     ccACGTTTGATGGAGAGATTAATCGGACCAACGGAAAACGCGTTATACTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGAGAGCGCGAATTT‑GTTCAGATTGTTAA‑ACGTCCCCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CAGCCGAAAGCAGCAGCGTACGCAGGAATAAAAGCGTCAACGAATAgatca                                                                                                                                                                      >  2:765495/1‑246 (MQ=255)                                                                                      gcccctcttccgatctataaaTCGGACAAACGAAAACCCCGTTACCCTGATAACAAAACGCCCGGTTCCGCAGCTCCAG‑CGTTT‑GCCAGTGGGGAACCGCCTGCGGGCGCGAAAACTCTG‑CTTTGAAAGGCGCGAGCGCGATTTT‑GTTCAGCTTGTTAA‑ACGCCCCCTGCGCCGTCAGCAGCGC‑CGGCAAGGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTcgcg                                                                                                                                                                    <  2:299491/232‑1 (MQ=255)                                                                                      cACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACATCACCTGCGCCGTCAGCAGCGA‑CGGCCAGGCGCCCA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAAGTcgcg                                                                                                                                                                    >  2:206660/1‑251 (MQ=255)                                                                                        gttttGATGGTGAGAATAATCGGACAAACGGAAAACCCGTTACCCTAAAAAGCAACTGTCACGTGCCGCAGCCCCAG‑CTTTT‑GCCAGTGGGGAAACCCCTGCGGGCGCGGAACCTCTG‑CTTTAAAAGGCGCGAGCGCAAATTT‑GTCCGGCTTGTAAA‑CCGTCCCCGGCGCCGTCAGCAGGGT‑CGGAAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGc                                                                                                                                                                  <  2:790980/249‑1 (MQ=255)                                                                                        ggTTTGATGGTGAAATTAATCGGACCAACGGAAACCGCGTTACCCTGTAAAACAAACGTCACGTTACGCAGCTCCAG‑CTTTT‑GCCAGTTGGGAACCGCCGGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTCCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGc                                                                                                                                                                  <  1:273834/250‑1 (MQ=255)                                                                                        cGTTTGATGGTGAGATTAATCGGACCAACGGAAACCGCGTTATCCTGATAAGCAACCGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGTTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAAGGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGc                                                                                                                                                                  <  1:244980/251‑1 (MQ=255)                                                                                        cGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGACACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGc                                                                                                                                                                  >  2:436125/1‑251 (MQ=255)                                                                                            tGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACg                                                                                                                                                              >  2:404153/1‑251 (MQ=255)                                                                                              aTGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGtt                                                                                                                                                            >  1:609110/1‑251 (MQ=255)                                                                                                 gTGAAATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGt                                                                                                                                                         <  1:72680/251‑1 (MQ=255)                                                                                                  tGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGt                                                                                                                                                         >  1:358196/1‑250 (MQ=255)                                                                                                  tGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCATCAACGAATAGGTCGCGGCAACGTTGGt                                                                                                                                                         >  2:250047/1‑250 (MQ=255)                                                                                                   gagaTTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTAt                                                                                                                                                       >  1:777536/1‑251 (MQ=255)                                                                                                      aTTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTcgcg                                                                                                                                                                    >  1:299491/1‑235 (MQ=255)                                                                                                       ttAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGAAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGc                                                                                                                                                   >  2:698026/1‑251 (MQ=255)                                                                                                        tAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAACGCAGCGGCGTACGCAGGAATAAACGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGcc                                                                                                                                                  >  2:405292/1‑251 (MQ=255)                                                                                                          aTCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGccc                                                                                                                                                 >  1:385403/1‑250 (MQ=255)                                                                                                                     ggAAACCGCGTTATCCTGAAAAGCAACCGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑CCGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGt                                                                                                                                     <  1:448417/251‑1 (MQ=255)                                                                                                                          aCGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGcc                                                                                                                                >  2:80621/1‑251 (MQ=255)                                                                                                                           cgcgTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGcc                                                                                                                                >  1:96692/1‑250 (MQ=255)                                                                                                                            gcgTTATCCTGATAAGCAAACGCCCCGTTACGCAGC‑CCAGGCGTTT‑GCCAGTGGGGAAACGCCTGCGGGCGCGGAAACCCTG‑CTTTGAAAGGCGCGAGCGGGAATTT‑GTTCAGTTTGTTAA‑ACGTCCCCTGCGCCGTCAGCAGCGG‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCAt                                                                                                                              <  1:394819/251‑1 (MQ=255)                                                                                                                                tatcctgaaacgaaaccGTCACGTTACGCAGCCCCAG‑TGTGT‑GCCAGTGGGAAAACGCTGGCGGGCGGGGAAAC‑CTGGTTTTGAAAGGCGCGAGCGCGAATTT‑GTCCAGCTGGTTAA‑CCGTCACCTGCCCCGTCACCAGGGC‑CGCAAAGGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCa                                                                                                                           <  2:169864/234‑1 (MQ=255)                                                                                                                                   cctaaTAAGCAAGCGCCCCGTTACGCAGCCCCA‑‑CGTTTTGCCATTGGGGAAACCCCGGCGGGCGCGGAACCTCTG‑CTTGGAAGGGCGCGAGCGCGATTTT‑GTCCAGCTTTTAAA‑ACGCCCCCTGCGCCTTCAGCAG‑GTGCGGCAATCCCCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAac                                                                                                                       <  2:760237/247‑1 (MQ=255)                                                                                                                                       aTAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAATGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACAcc                                                                                                                    >  2:482312/1‑250 (MQ=255)                                                                                                                                         aaGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAgg                                                                                                                 <  1:6594/251‑1 (MQ=255)                                                                                                                                         aaGCAAACGTCACGATACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCCTACAGACCACCAgg                                                                                                                 >  2:447266/1‑251 (MQ=255)                                                                                                                                            cAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGAGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGACGTCAGCAGCGC‑CGGCAATGCGCCAA‑ACGCCGAAAGCAGCGGCGTACGAAGCACTAAAAGAGACAACGAATAGGACGCGACACCGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCg                                                                                                              >  1:373229/1‑251 (MQ=255)                                                                                                                                             aactggcACGTTCCGCAGCCCCAG‑CGTTG‑GCCAGGTGGGAACCGCCCGGGGGCGGGGAAACTGTG‑TTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTAAA‑ACCACCCCGGCGCCGCCAGCGGCGG‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGa                                                                                                             <  2:23501/245‑1 (MQ=255)                                                                                                                                                  ccgggttccacggcgccgg‑CGTTT‑GCCGGTGGGGAACCGCCGGCGGGCCGGGAACCTCTG‑TTTGGAAGGGCGCGGGGGAGAATTT‑GTTCAGTGTGTAAA‑CCGTCACCTGCGCCGTCAGCGGGGC‑CGGAAAGGCGCAAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTgcg                                                                                                        <  2:171503/233‑1 (MQ=255)                                                                                                                                                     cGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGccc                                                                                                     >  2:533108/1‑251 (MQ=255)                                                                                                                                                      gTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGGATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGcccc                                                                                                    >  2:569860/1‑250 (MQ=255)                                                                                                                                                        tACGCAGCTCCAG‑CGTTT‑GCCAGTGGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGCCACCGGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAg                                                                                                   <  1:541024/250‑1 (MQ=255)                                                                                                                                                             aGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGcatcat                                                                                             >  1:189522/1‑251 (MQ=255)                                                                                                                                                                       gTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTg                                                                                     >  1:502422/1‑250 (MQ=255)                                                                                                                                                                        gttgtccatttgggacccGCCTGCGGGCCCGAAATCTCTG‑CTTTAAGAGGCGCGGGCGAGATGTT‑TTTCAG‑TTTTTAAGACGCCCCCTCCGCCGTCCGCTGCGT‑CGACAATGCCCCCT‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACTCCAGGCCGATTGCGCCCAGCATCatgacgattgc                                                                                    <  2:60149/234‑8 (MQ=255)                                                                                                                                                                                  ggggAAACGCCTGCGGGCGCGGAACCTTTG‑CTTTGAAAGGCGCGGGCGCAAATTT‑GTTCAGCTTGTTAA‑CCGCCCCCTGCGCCGTCAGCAGCGT‑TGCAAATGCGCAAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTAGACCAGTTCAc                                                                           <  2:479080/249‑1 (MQ=255)                                                                                                                                                                                   gggAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACgg                                                                         <  1:80621/251‑1 (MQ=255)                                                                                                                                                                                        aCGCCTGCGGGCGCGGAACCGCTG‑TTTTGAAAGGCGCGAGCGGGATTTT‑GTTCAGCTTGGAAA‑ACGTCCCCTGCGCCGTCAGGAGCGT‑CGGAAATGCCCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTa                                                                    <  1:206660/251‑1 (MQ=255)                                                                                                                                                                                         cgcatGCGGGCGCGGAAAATATG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAC‑ACGTCAACTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCAGAAAGCAGCGGCGTACGCAGGCATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCACAACCGACGCTGTTAGCCATCCAGAACACCAGGCCGATTGAGCCCAGAATCATGATGTTTGATCAGTTCAAGGCaata                                                                    >  2:361623/5‑247 (MQ=255)                                                                                                                                                                                           ccTGGGGGCGCGGAACCTCTG‑CTTTAAAAGGCGCGCGCGCGTATTT‑GCTCCGCTTGTTAA‑ACGCCCCCTGCGCCGCCAGCGTCGT‑CGGCAATGCGCCAC‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGa                                                                 <  2:790610/251‑1 (MQ=255)                                                                                                                                                                                              gggggCGCTGAACCTCTG‑TTTTGAAGGGCGCGA‑CCCGAATTTTGTTCAGCTTGTAAA‑CCGCCCCCTGCCCCGTCAGCAGCGT‑CGCCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATgg                                                              <  2:601889/249‑1 (MQ=255)                                                                                                                                                                                              gCGGGCGCGGAAACTCTG‑TTTTGAAAGGCGCGGGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATgg                                                              <  1:474434/251‑1 (MQ=255)                                                                                                                                                                                              gCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGGGCGCGAATTT‑GTTCAGCTTGTTAA‑CCGTCCCCTGCGCCGCCAGCAGCGT‑CGGAAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATgg                                                              <  1:81135/251‑1 (MQ=255)                                                                                                                                                                                              gCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTTTTAA‑ACGTCCCCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGAt                                                                <  1:778520/249‑1 (MQ=255)                                                                                                                                                                                              gCGGGCGCGAAAACTGTG‑CTTTGAAAGGCGCGCGCGCGAATTT‑GTTCAGCTTGTTAA‑CCGGCCCCGGCGCCGGCAGCAGCGG‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAAAAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGAt                                                                <  2:138842/249‑1 (MQ=255)                                                                                                                                                                                                      ggAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCCCTACGCTGGAAGGTGTc                                                      >  2:137038/1‑251 (MQ=255)                                                                                                                                                                                                          aCTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑CCGCCCCCGGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGAGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTgcg                                                  <  1:60215/251‑1 (MQ=255)                                                                                                                                                                                                                  tttGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGCCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCAATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGaata                                            >  2:318474/1‑250 (MQ=255)                                                                                                                                                                                                                      aaaGGCGCGAGCGCGATTTT‑GTTCAGCTTGTTAA‑ACGTCCCCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATAtg                                       <  1:482312/251‑1 (MQ=255)                                                                                                                                                                                                                        aGGCGGGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACTCCCCCGGCGCCGTCAGCAGCGG‑CGGCAAGGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATAtggt                                     <  1:135044/251‑1 (MQ=255)                                                                                                                                                                                                                               ggcgcgaTTTT‑GTTAAGCTGGTAAA‑CCGTCCCCGGCGCCGTCAGCAGGGT‑CGGCAAGGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATa                              <  2:385403/250‑1 (MQ=255)                                                                                                                                                                                                                               ggcgcgaATTT‑GTCCAGCTTGTTAA‑ACGCCCCCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCTGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATa                              <  1:419416/250‑1 (MQ=255)                                                                                                                                                                                                                               agcgcgaATTT‑GTTCAGCTTGTTAA‑ACGTCACCTTCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGCGGCTGTTCGCCCTCCAGAACCCAATGCCACTTGCGCCCAGCATCATGATGTTTGCCCAGTTCACGGCACTAAGATGGCAGGAGTCATCACGACTCATATGGTGGCgctc                              >  2:565024/1‑248 (MQ=255)                                                                                                                                                                                                                                     aaTTT‑GTTCAGCTTGTTAA‑ACGCCCCCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCAATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACACTTg                        <  1:318474/251‑1 (MQ=255)                                                                                                                                                                                                                                             tCAGCTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTGCGCATc                  >  1:376003/1‑250 (MQ=255)                                                                                                                                                                                                                                                 cTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTGCGCATCAGGa              >  1:347386/1‑250 (MQ=255)                                                                                                                                                                                                                                                 cTTGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTGCGCATCAGGa              >  1:143150/1‑250 (MQ=255)                                                                                                                                                                                                                                                  ttGTTAA‑ACGTCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTGCGCATCAGGAAt            >  2:372132/1‑251 (MQ=255)                                                                                                                                                                                                                                                     ttAA‑ACGTCACCTGCGCCGTCAGCAGCTT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTGCGCATCAGGAATGt          <  1:580636/250‑1 (MQ=255)                                                                                                                                                                                                                                                       aa‑aCGTCACCTGCGCCGTCAGCAGCGT‑CGGCCATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCAAACCGGAGCTGTTCGCCATCCAGAACACCACGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTGCGCATCAGGAATGt          >  1:310840/1‑248 (MQ=255)                                                                                                                                                                                                                                                             ccACCTGCCCCGACAGCAGCGA‑CGGCA‑TGTACCAATCCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGGTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCTCTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACGCTTGCGCATCAGGAATGTAGAGgttg  <  2:691951/251‑1 (MQ=255)                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                             CTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAGCTCCAG‑CGTTT‑GCCAGTTGGGAAACGCCTGCGGGCGCGGAAACTCTG‑CTTTGAAAGGCGCGAGCGCGAATTT‑GTTCAGCTTGTTAA‑ACGCCACCTGCGCCGTCAGCAGCGT‑CGGCAATGCGCCAA‑CCGCCGAAAGCAGCGGCGTACGCAGGAATAAAAGCGTCAACGAATAGGTCGCGGCAACGTTGGTATCAGCCCAACCGAGGCTGTTCGCCATCCAGAACACCAGGCCGATTGCGCCCAGCATCATGATGTTTGACCAGTTCACGGCACTAAGATGGAAGGTGTCTGCGCGAATAATATGGTGGCGATACTCTTGCGCATCAGGAATGTAGAGGTTG  >  NC_000913/2307486‑2307983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑