breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 109,960 G→A R561H (CGT→CAT)  secA → protein translocation ATPase
RA 113,438 C→T intergenic (+150/‑6) yacM → / → guaC protein YacM/GMP reductase
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
RA 334,188 G→A I82I (ATC→ATT yahB ← putative LysR‑type DNA‑binding transcriptional regulator YahB
RA 339,935 C→T A56V (GCC→GTC)  yahH → putative uncharacterized protein YahH
RA 339,941 C→T T58I (ACT→ATT)  yahH → putative uncharacterized protein YahH
RA 339,958 G→A D64N (GAC→AAC)  yahH → putative uncharacterized protein YahH
RA 339,979 G→A V71M (GTG→ATG)  yahH → putative uncharacterized protein YahH
RA 339,986 G→A R73Q (CGA→CAA)  yahH → putative uncharacterized protein YahH
JC JC 362,107 IS5 (+) +4 bp coding (1070‑1073/1254 nt) lacY ← lactose permease
RA 466,169 T→A T382S (ACC→TCC)  ybaE ← uncharacterized protein YbaE
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
JC JC 480,735 IS5 (+) +4 bp intergenic (‑27/+516) tomB ← / ← acrB Hha toxicity modulator TomB/multidrug efflux pump RND permease AcrB
RA 631,618 Δ1 bp coding (1725/2106 nt) cstA → pyruvate transporter CstA
MC JC 678,921 Δ8,930 bp IS5‑mediated [ybeT]gltI [ybeT], ybeU, djlC, hscC, rihA, gltL, gltK, gltJ, sroC, gltI
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
JC JC 792,077 IS2 (+) +5 bp intergenic (‑22/+235) galE ← / ← modF UDP‑glucose 4‑epimerase/ABC family protein ModF
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase
RA 1,196,316 T→A D398E (GAT→GAA icd → isocitrate dehydrogenase
RA 1,196,325 A→G K401K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,352 C→A D410E (GAC→GAA icd → isocitrate dehydrogenase
JC JC 1,217,464 IS2 (–) +5 bp coding (137‑141/1524 nt) pdeG → putative c‑di‑GMP phosphodiesterase PdeG
RA 1,290,644 G→T E135* (GAA→TAA)  rssB → regulator of RpoS
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,381,704 A→C intergenic (+897/‑243) ycjU → / → ompG beta‑phosphoglucomutase/outer membrane porin G
RA 1,383,033 A→T L310* (TTA→TAA)  ycjW ← DNA‑binding transcriptional repressor YcjW
JC JC 1,435,353 IS2 (+) +5 bp coding (263‑267/435 nt) uspF ← universal stress protein F
RA 1,449,061 T→G intergenic (+43/+15) feaB → / ← tynA phenylacetaldehyde dehydrogenase/copper‑containing amine oxidase
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
JC JC 1,656,167 IS2 (–) +5 bp intergenic (+18/‑13) ynfB → / → speG DUF1283 domain‑containing protein YnfB/spermidine N‑acetyltransferase
JC JC 1,671,703 IS2 (–) +5 bp intergenic (+19/‑70) asr → / → ydgU periplasmic chaperone Asr/uncharacterized protein YdgU
RA 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI
JC JC 1,743,490 IS5 (+) +4 bp coding (34‑37/1374 nt) mdtK → multidrug efflux pump MdtK
RA 1,776,715 A→T S377C (AGT→TGT)  ydiF → putative acetate‑CoA transferase YdiF
RA 1,856,968 T→A I268F (ATT→TTT)  ydjI ← L‑glycero‑L‑galacto‑octuluronate‑1‑phosphate aldolase
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,987,499 C→A intergenic (+71/+8) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,096,437 Δ1 bp coding (594/777 nt) hisF → imidazole glycerol phosphate synthase subunit HisF
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,188,087 T→C T110A (ACG→GCG)  yehA ← putative fimbrial adhesin YehA
RA 2,239,760 C→T E197K (GAA→AAA)  mglB ← D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
JC JC 2,452,057 IS2 (–) +5 bp coding (276‑280/753 nt) yfcS ← putative fimbrial chaperone YfcS
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
JC JC 2,788,791 IS5 (–) +4 bp intergenic (‑2954/‑191) ileY ← / → glaH tRNA‑Ile/glutarate dioxygenase GlaH
MC JC 2,867,280 Δ8 bp coding (265‑272/993 nt) rpoS ← RNA polymerase sigma factor RpoS
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
RA 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter
RA 3,101,039 (C)7→8 coding (585/720 nt) yggN ← DUF2884 domain‑containing protein YggN
RA 3,263,231 T→A T144S (ACC→TCC)  tdcD ← propionate kinase
RA 3,308,048 G→A Q293* (CAG→TAG)  nlpI ← lipoprotein NlpI
RA 3,316,428 C→T E367K (GAA→AAA)  nusA ← transcription termination/antitermination protein NusA
RA 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
JC JC 3,367,057 IS2 (–) +5 bp coding (132‑136/717 nt) yhcF → DUF1120 domain‑containing protein YhcF
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
JC JC 3,437,349 IS30 (–) +2 bp coding (832‑833/1377 nt) trkA → NAD‑binding component of Trk potassium transporters
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,475,993 T→C T150A (ACC→GCC)  yheO ← DNA‑binding transcriptional regulator YheO
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,566,550 G→A T11M (ACG→ATG)  glgP ← glycogen phosphorylase
RA 3,629,921 C→T E228K (GAG→AAG)  yhiI ← putative membrane fusion protein YhiI
JC JC 3,638,509 IS5 (–) +4 bp coding (868‑871/1500 nt) pitA → metal phosphate:H(+) symporter PitA
RA 3,682,515 C→T M311I (ATG→ATA dctA ← C4 dicarboxylate/orotate:H(+) symporter
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,761,230 G→A S7F (TCC→TTC)  selA ← selenocysteine synthase
JC JC 3,766,086 IS5 (+) +4 bp coding (3904‑3907/4134 nt) rhsA → rhs element protein RhsA
RA 3,771,979 G→T intergenic (‑235/‑302) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
JC 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
JC JC 3,828,674 IS2 (–) +5 bp intergenic (‑9/‑267) gltS ← / → xanP glutamate:sodium symporter/xanthine:H(+) symporter XanP
RA 3,869,637 C→A L87L (CTC→CTA cbrA → colicin M resistance protein
RA 3,878,536 G→A Y528Y (TAC→TAT gyrB ← DNA gyrase subunit B
RA 3,912,074 G→A S532F (TCC→TTC)  glmS ← L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
MC JC 3,933,612 Δ174 bp [rbsD][rbsA] [rbsD], [rbsA]
JC JC 3,939,119 IS2 (+) +5 bp coding (893‑897/993 nt) rbsR → DNA‑binding transcriptional dual regulator RbsR
RA 3,967,066 C→T T217I (ACC→ATC)  rho → transcription termination factor Rho
RA 3,993,531 T→C R793R (CGT→CGC cyaA → adenylate cyclase
RA 3,996,049 T→A L156* (TTG→TAG)  yigA → DUF484 domain‑containing protein YigA
RA 4,011,802 C→T P243S (CCG→TCG)  bioP → biotin transporter
RA 4,031,457 C→T F99F (TTC→TTT pepQ → Xaa‑Pro dipeptidase
RA 4,031,511 C→T S117S (AGC→AGT pepQ → Xaa‑Pro dipeptidase
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,161,248 G→T G61V (GGC→GTC)  fabR → DNA‑binding transcriptional repressor FabR
RA 4,164,123 C→G A162G (GCA→GGA)  btuB → cobalamin outer membrane transporter
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,254,787 G→A P560P (CCC→CCT plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,254,790 C→T V559V (GTG→GTA plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,254,865 T→C A534A (GCA→GCG plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,254,871 T→A L532L (CTA→CTT plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,015 A→T P484P (CCT→CCA plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,021 A→G H482H (CAT→CAC plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,198 A→G Y423Y (TAT→TAC plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,270 A→G T399T (ACT→ACC plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,297 A→G R390R (CGT→CGC plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,366 C→A R367R (CGG→CGT plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,402 C→A T355T (ACG→ACT plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,450 A→C P339P (CCT→CCG plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,459 A→G N336N (AAT→AAC plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,474 G→A A331A (GCC→GCT plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,543 A→G S308S (AGT→AGC plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,606 A→G A287A (GCT→GCC plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,636 T→G R277R (CGA→CGC plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,717 T→C A250A (GCA→GCG plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,839 A→G L210L (TTG→CTG)  plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,849 T→C V206V (GTA→GTG plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,852 G→A A205A (GCC→GCT plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,255,906 A→T A187A (GCT→GCA plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,256,002 A→G A155A (GCT→GCC plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,264,742 C→T A143A (GCC→GCT dnaB → replicative DNA helicase
RA 4,264,748 T→C A145A (GCT→GCC dnaB → replicative DNA helicase
RA 4,264,790 T→C D159D (GAT→GAC dnaB → replicative DNA helicase
RA 4,264,793 G→T L160L (CTG→CTT dnaB → replicative DNA helicase
RA 4,264,832 G→A A173A (GCG→GCA dnaB → replicative DNA helicase
RA 4,264,931 G→A G206G (GGG→GGA dnaB → replicative DNA helicase
RA 4,265,078 G→A P255P (CCG→CCA dnaB → replicative DNA helicase
RA 4,265,105 A→T P264P (CCA→CCT dnaB → replicative DNA helicase
RA 4,265,117 C→T I268I (ATC→ATT dnaB → replicative DNA helicase
RA 4,265,186 C→T D291D (GAC→GAT dnaB → replicative DNA helicase
RA 4,266,360 G→A A193A (GCG→GCA alr → alanine racemase 1
RA 4,266,534 G→A E251E (GAG→GAA alr → alanine racemase 1
RA 4,267,333 T→A C74S (TGC→AGC)  tyrB → tyrosine aminotransferase
RA 4,269,615 T→G F68V (TTT→GTT)  aphA → acid phosphatase/phosphotransferase
RA 4,271,744 G→A P710S (CCT→TCT)  uvrA ← UvrABC excision nuclease subunit A
RA 4,271,781 T→C P697P (CCA→CCG uvrA ← UvrABC excision nuclease subunit A
RA 4,271,823 A→G D683D (GAT→GAC uvrA ← UvrABC excision nuclease subunit A
RA 4,271,838 C→T G678G (GGG→GGA uvrA ← UvrABC excision nuclease subunit A
RA 4,271,862 T→C P670P (CCA→CCG uvrA ← UvrABC excision nuclease subunit A
RA 4,271,955 G→T T639T (ACC→ACA uvrA ← UvrABC excision nuclease subunit A
RA 4,271,976 C→G V632V (GTG→GTC uvrA ← UvrABC excision nuclease subunit A
RA 4,271,979 C→T P631P (CCG→CCA uvrA ← UvrABC excision nuclease subunit A
RA 4,272,000 C→T K624K (AAG→AAA uvrA ← UvrABC excision nuclease subunit A
RA 4,272,009 G→A N621N (AAC→AAT uvrA ← UvrABC excision nuclease subunit A
RA 4,272,012 G→A G620G (GGC→GGT uvrA ← UvrABC excision nuclease subunit A
RA 4,272,090 G→A G594G (GGC→GGT uvrA ← UvrABC excision nuclease subunit A
RA 4,272,105 C→T G589G (GGG→GGA uvrA ← UvrABC excision nuclease subunit A
RA 4,272,111 C→T L587L (TTG→TTA uvrA ← UvrABC excision nuclease subunit A
RA 4,272,135 C→T A579A (GCG→GCA uvrA ← UvrABC excision nuclease subunit A
RA 4,272,144 C→T P576P (CCG→CCA uvrA ← UvrABC excision nuclease subunit A
RA 4,272,153 T→C A573A (GCA→GCG uvrA ← UvrABC excision nuclease subunit A
RA 4,272,222 T→C A550A (GCA→GCG uvrA ← UvrABC excision nuclease subunit A
RA 4,272,306 A→G R522R (CGT→CGC uvrA ← UvrABC excision nuclease subunit A
RA 4,272,327 C→T P515P (CCG→CCA uvrA ← UvrABC excision nuclease subunit A
RA 4,272,396 T→G A492A (GCA→GCC uvrA ← UvrABC excision nuclease subunit A
RA 4,272,408 A→G S488S (TCT→TCC uvrA ← UvrABC excision nuclease subunit A
RA 4,272,473 G→A L467L (CTG→TTG)  uvrA ← UvrABC excision nuclease subunit A
RA 4,272,495 G→A I459I (ATC→ATT uvrA ← UvrABC excision nuclease subunit A
RA 4,272,504 T→C A456A (GCA→GCG uvrA ← UvrABC excision nuclease subunit A
RA 4,272,564 A→G I436I (ATT→ATC uvrA ← UvrABC excision nuclease subunit A
RA 4,272,582 A→G A430A (GCT→GCC uvrA ← UvrABC excision nuclease subunit A
RA 4,272,585 A→G P429P (CCT→CCC uvrA ← UvrABC excision nuclease subunit A
RA 4,272,639 C→T G411G (GGG→GGA uvrA ← UvrABC excision nuclease subunit A
RA 4,272,657 C→T P405P (CCG→CCA uvrA ← UvrABC excision nuclease subunit A
RA 4,272,660 A→G R404R (CGT→CGC uvrA ← UvrABC excision nuclease subunit A
RA 4,272,666 A→G S402S (AGT→AGC uvrA ← UvrABC excision nuclease subunit A
RA 4,272,690 G→A R394R (CGC→CGT uvrA ← UvrABC excision nuclease subunit A
RA 4,272,699 G→A S391S (AGC→AGT uvrA ← UvrABC excision nuclease subunit A
RA 4,272,708 C→T T388T (ACG→ACA uvrA ← UvrABC excision nuclease subunit A
RA 4,272,720 G→A R384R (CGC→CGT uvrA ← UvrABC excision nuclease subunit A
RA 4,272,735 A→G H379H (CAT→CAC uvrA ← UvrABC excision nuclease subunit A
RA 4,272,765 A→G I369I (ATT→ATC uvrA ← UvrABC excision nuclease subunit A
RA 4,272,857 G→A L339L (CTG→TTG)  uvrA ← UvrABC excision nuclease subunit A
RA 4,272,903 C→T S323S (TCG→TCA uvrA ← UvrABC excision nuclease subunit A
RA 4,272,963 A→T A303A (GCT→GCA uvrA ← UvrABC excision nuclease subunit A
RA 4,272,993 T→G R293R (CGA→CGC uvrA ← UvrABC excision nuclease subunit A
RA 4,272,996 A→G D292D (GAT→GAC uvrA ← UvrABC excision nuclease subunit A
RA 4,273,086 T→C E262E (GAA→GAG uvrA ← UvrABC excision nuclease subunit A
RA 4,273,089 A→G R261R (CGT→CGC uvrA ← UvrABC excision nuclease subunit A
RA 4,273,101 G→A G257G (GGC→GGT uvrA ← UvrABC excision nuclease subunit A
RA 4,273,197 C→T L225L (CTG→CTA uvrA ← UvrABC excision nuclease subunit A
RA 4,273,212 T→C S220S (TCA→TCG uvrA ← UvrABC excision nuclease subunit A
RA 4,273,221 A→C L217L (CTT→CTG uvrA ← UvrABC excision nuclease subunit A
RA 4,273,302 T→C P190P (CCA→CCG uvrA ← UvrABC excision nuclease subunit A
RA 4,273,539 T→C L111L (TTA→TTG uvrA ← UvrABC excision nuclease subunit A
RA 4,274,110 (T)7→8 intergenic (‑239/‑15) uvrA ← / → ssb UvrABC excision nuclease subunit A/ssDNA‑binding protein
RA 4,274,277 A→G E51E (GAA→GAG ssb → ssDNA‑binding protein
RA 4,274,328 G→C A68A (GCG→GCC ssb → ssDNA‑binding protein
RA 4,274,499 C→T G125G (GGC→GGT ssb → ssDNA‑binding protein
RA 4,274,653 C→T P177S (CCG→TCG)  ssb → ssDNA‑binding protein
RA 4,282,332 (A)5→6 coding (744/1293 nt) yjcF ← pentapeptide repeat‑containing protein YjcF
RA 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,392,997 G→A V357V (GTC→GTT queG ← epoxyqueuosine reductase
RA 4,393,552 G→A F172F (TTC→TTT queG ← epoxyqueuosine reductase
RA 4,396,373 C→T T103T (ACC→ACT amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,396,772 T→C F236F (TTT→TTC amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,424,009 C→A Q104K (CAG→AAG)  ulaF → L‑ribulose‑5‑phosphate 4‑epimerase UlaF
RA 4,425,688 C→A H57N (CAC→AAC)  priB → primosomal replication protein N
RA 4,425,696 C→A N59K (AAC→AAA priB → primosomal replication protein N
RA 4,499,661 T→C intergenic (+161/‑1599) insD6 → / → yjgZ IS2 insertion element protein InsB/uncharacterized protein YjgZ
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,518,627 2 bp→AC coding (101‑102/276 nt) insA7 → IS1 family repressor protein InsA
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196375 1211479 15105 11 [0] [10] 11 C0293–mcrA C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270 776 68 [0] [0] 73 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3134043 3136514 2472 82 [0] [0] 96 yghT–pitB yghT,pitB
* * ÷ NC_000913 3423925–3424231 3424437–3424239 9–513 12 [10] [10] 11 rrlD 23S ribosomal RNA
* * ÷ NC_000913 3708467 3708562 96 36 [0] [8] 12 dppA/proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
* * ÷ NC_000913 4035421–4035733 4036872–4035735 3–1452 11 [10] [10] 12 [rrsA] [rrsA]
* * ÷ NC_000913 4640415 4640417 3 87 [0] [0] 109 yjjY uncharacterized protein YjjY

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1397238NA (NA)68 (0.970) 45/590 0.3 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 = 1469241NA (NA)80 (1.170)
+GTACGTAA
59/574 0.1 NA intergenic (‑92/‑5) insC2/ydbA IS2 insertion element repressor InsA/putative outer membrane protein, N‑terminal fragment
?NC_000913 1469295 = NA (NA)pseudogene (50/50 nt) ydbA putative outer membrane protein, N‑terminal fragment
* ? NC_000913 1979271 =0 (0.000)73 (1.040) 52/590 0.2 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 = 2290113NA (NA)82 (1.170) 62/590 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3134042 0 (0.000)intergenic (‑85/‑89) yghS/yghT putative ATP‑binding protein YghS/putative ATP‑binding protein YghT
* ? NC_000913 = 3131340NA (NA)96 (1.370) 62/590 0.1 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 3136515 = 0 (0.000)intergenic (‑144/+148) pitB/gss metal phosphate:H(+) symporter PitB/fused glutathionylspermidine amidase/glutathionylspermidine synthetase
* ? NC_000913 = 37084660 (0.000)36 (0.810) 22/374 0.6 100% intergenic (‑761/+150) dppA/proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
?NC_000913 3708635 = 0 (0.000)noncoding (58/77 nt) proK tRNA‑Pro
* ? NC_000913 4498180 =NA (NA)33 (0.470) 25/590 1.1 NA intergenic (+1691/‑92) leuX/insC6 tRNA‑Leu/IS2 insertion element repressor InsA
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 4499511NA (NA)87 (1.240) 58/590 0.1 100% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 4640414 0 (0.000)coding (13/141 nt) yjjY uncharacterized protein YjjY
* ? NC_000913 4507459 =NA (NA)108 (1.550) 68/588 0.0 100% noncoding (1221/1221 nt) IS30 repeat region
?NC_000913 4640418 = 0 (0.000)coding (17/141 nt) yjjY uncharacterized protein YjjY
* ? NC_000913 4542682 =114 (1.630)26 (0.380) 25/572 1.1 22.5% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 69 (1.020)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 4542690119 (1.700)32 (0.470) 26/572 1.0 25.8% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 69 (1.020)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit