Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,255,852	G→A	A205A (GCC→GCT)	plsB ←	glycerol‑3‑phosphate 1‑O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,255,852	0	G	A	100.0%	195.3 / NA	58	A205A (GCC→GCT)	plsB	glycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/31);  total (27/31)

CATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC  >  NC_000913/4255560‑4256139                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                caTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATAAGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCa                                                                                                                                                                                                                                                                                          >  2:498372/1‑300 (MQ=255)  atatACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGANGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCa                                                                                                                                                                                                                                                                                          <  2:441023/299‑1 (MQ=255)      aCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGt                                                                                                                                                                                                                                                                                     <  1:148268/300‑1 (MQ=255)              aTGACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCACCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGc                                                                                                                                                                                                                                                                             <  2:59421/300‑1 (MQ=255)                gACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCaa                                                                                                                                                                                                                                                                           <  1:483730/300‑1 (MQ=255)                    gtcgtGGGCCAGCTGNCGAACGCGCTCGGCGTTANGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCANACGTTGACGGGCAAAg                                                                                                                                                                                                                                                                         <  1:473731/298‑1 (MQ=255)                         gggCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATAc                                                                                                                                                                                                                                                                  <  1:274592/300‑1 (MQ=255)                          ggCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATAcg                                                                                                                                                                                                                                                                 <  1:50026/300‑1 (MQ=255)                          ggCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATAcg                                                                                                                                                                                                                                                                 <  1:50050/300‑1 (MQ=255)                              aGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATAcgcg                                                                                                                                                                                                                                                               <  1:420366/298‑1 (MQ=255)                                   gCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCAcgcg                                                                                                                                                                                                                                                        <  1:235539/300‑1 (MQ=255)                                             tcggCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTc                                                                                                                                                                                                                                              <  1:200582/297‑1 (MQ=255)                                                  gTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTgtgc                                                                                                                                                                                                                                         >  1:17534/1‑297 (MQ=255)                                                  gTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTgtgc                                                                                                                                                                                                                                         >  1:20124/1‑297 (MQ=255)                                                     aTGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTACATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCACGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAATGCATACGCGACACGCGCGCCAGTTTCTgtacact                                                                                                                                                                                                                                      >  2:278445/1‑294 (MQ=255)                                                            ttGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGCCTCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCACTCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGAGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCCTACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTa                                                                                                                                                                                                                               >  1:365536/1‑300 (MQ=255)                                                              gATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCATGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATc                                                                                                                                                                                                                             <  1:439567/300‑1 (MQ=255)                                                                 gCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATccgt                                                                                                                                                                                                                          >  2:319830/1‑300 (MQ=255)                                                                                 ttCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCATTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGAAGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGca                                                                                                                                                                                                          >  2:211/1‑299 (MQ=255)                                                                                        tGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATTGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGgc                                                                                                                                                                                                   <  2:477290/300‑1 (MQ=255)                                                                                        tGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGgc                                                                                                                                                                                                   <  1:12525/300‑1 (MQ=255)                                                                                            gCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCa                                                                                                                                                                                                >  1:89534/1‑299 (MQ=255)                                                                                                aGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGa                                                                                                                                                                                            <  2:386088/299‑1 (MQ=255)                                                                                                 gAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGaaa                                                                                                                                                                                          >  1:306803/1‑300 (MQ=255)                                                                                                 gAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGaa                                                                                                                                                                                           >  2:533352/1‑299 (MQ=255)                                                                                                    tACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTg                                                                                                                                                                                       <  1:49166/300‑1 (MQ=255)                                                                                                       ggTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGTAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCATGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACg                                                                                                                                                                                    <  2:243615/300‑1 (MQ=255)                                                                                                            gtcagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCga                                                                                                                                                                                >  1:140663/1‑299 (MQ=255)                                                                                                            gtcagGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCga                                                                                                                                                                                >  1:140920/1‑299 (MQ=255)                                                                                                                ggCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGTTGCGCTTTTTAATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGTAGCTTATTAAACAGATCCTGCCGAGCAGGCAGACGTGGGCCCACAGCAGCCGGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGACATATGGCGCGGCGGAACTGACAGCga                                                                                                                                                                                >  2:192215/1‑295 (MQ=255)                                                                                                                   gAATCATCTAGTAAGANAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCNCCATACGGCGCAGCGAAANTGACGGCGAGAAACGca                                                                                                                                                                        >  1:475959/1‑300 (MQ=255)                                                                                                                                   gaAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGAcc                                                                                                                                                        <  1:453502/300‑1 (MQ=255)                                                                                                                                             gcgaTCTCTTCCATCGACGCAATCGCGTTCTGCTGCGCTTTGTCATTGGAGATTTTTCTGCTGCGCGCTTCATCTTCTACCGCTGTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCATGCAGACGTGGGCCCACAGCAGCCAGACGTTGATGGGCAAAGTGCATACGCGCCACGCGCGCCAATTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAg                                                                                                                                               <  2:68795/296‑1 (MQ=255)                                                                                                                                               aaTCTCTTCCATCAGCGNAATCGCGTTCTGCTGCGCNTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGNGACCGAGCCACAGTAc                                                                                                                                            <  1:462474/300‑1 (MQ=255)                                                                                                                                               aaTCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                                                                            <  1:155366/300‑1 (MQ=255)                                                                                                                                                  ctctTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGCCGCGACCGAGCCACAGTAcggc                                                                                                                                         >  2:518895/1‑297 (MQ=255)                                                                                                                                                           cagcgCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAAttt                                                                                                                                >  1:218806/5‑300 (MQ=255)                                                                                                                                                             gcgCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCt                                                                                                                              >  1:148331/3‑300 (MQ=255)                                                                                                                                                             gcgCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGACACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCt                                                                                                                              >  2:384572/3‑300 (MQ=255)                                                                                                                                                                            gctgcGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCa                                                                                                               <  1:505278/300‑1 (MQ=255)                                                                                                                                                                               gcgcTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATAc                                                                                                            >  1:487152/1‑300 (MQ=255)                                                                                                                                                                                                           gcgcgcTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                                                                            <  1:230458/240‑1 (MQ=255)                                                                                                                                                                                                           gcgcgcTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                                                                            <  1:229915/240‑1 (MQ=255)                                                                                                                                                                                                           gcgcgcTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                                                                            >  2:230458/1‑240 (MQ=255)                                                                                                                                                                                                           gcgcgcTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCAACAGTAc                                                                                                                                            >  2:229915/1‑240 (MQ=255)                                                                                                                                                                                                                    aTCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCAACAGTACGGCGAAAAATTTATGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGccca                                                                         >  2:311560/1‑297 (MQ=255)                                                                                                                                                                                                                                         ggCGCGGGAGGCGAGTAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACACGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGAACGAGCAACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCAGGCGCGCGACCAAACATCAcc                                                    >  2:297771/1‑298 (MQ=255)                                                                                                                                                                                                                                         ggCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCAACGa                                                  >  2:299968/1‑300 (MQ=255)                                                                                                                                                                                                                                                  ggCGAGCGGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACACGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACTGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTCCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACACCACCGACACTGGCAc                                         <  2:40027/300‑1 (MQ=255)                                                                                                                                                                                                                                                  ggCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCAc                                         <  1:14323/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     aGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGTGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAtt                       <  2:503608/299‑1 (MQ=255)                                                                                                                                                                                                                                                                     aGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAtt                       <  1:311560/299‑1 (MQ=255)                                                                                                                                                                                                                                                                      gATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAttt                      >  1:420824/1‑299 (MQ=255)                                                                                                                                                                                                                                                                       aTCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCAACGACACTGGCACCATCTGCACCTCCAGAttt                      >  2:525995/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                 gcaggcagACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACgg          <  1:386448/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    ggCAGACGTGGGCACACAGCAGCCATACGGTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGNACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGc       <  2:400383/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         aCGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATc  <  1:515832/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          cGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACTCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATc  >  1:338340/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                CATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATC  >  NC_000913/4255560‑4256139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑