Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,436,697	G→A	E60E (GAG→GAA)	trkA →	NAD‑binding component of Trk potassium transporters

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,436,697	0	G	A	100.0%	205.1 / NA	60	E60E (GAG→GAA)	trkA	NAD‑binding component of Trk potassium transporters
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/25);  total (35/25)

GGAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGAACTTCGCTGAGGGTAAAGTCAGCCTGGCTG  >  NC_000913/3436410‑3436986                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                    ggAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCcgac                                                                                                                                                                                                                                                                                         <  1:524386/300‑1 (MQ=255)  gAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGcc                                                                                                                                                                                                                                                                                            <  2:467418/296‑1 (MQ=255)  gAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTAAAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGTcgac                                                                                                                                                                                                                                                                                         >  2:494437/1‑299 (MQ=255)                            aaTCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAAcc                                                                                                                                                                                                                                                              <  1:263511/300‑1 (MQ=255)                                      gTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGAt                                                                                                                                                                                                                                                     >  1:200455/1‑299 (MQ=255)                                                gggCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACAAatat                                                                                                                                                                                                                                          >  2:280462/1‑300 (MQ=255)                                                gggCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGAACTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAatct                                                                                                                                                                                                                                          >  2:461941/1‑298 (MQ=255)                                                gggCGAAGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAatat                                                                                                                                                                                                                                          <  1:288794/300‑1 (MQ=255)                                                     aCGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGNGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATTAAACCAATATGGTtg                                                                                                                                                                                                                                     <  2:406114/300‑1 (MQ=255)                                                            cttTTACGCTAAGCTAATCAAAAAGTGATTAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCa                                                                                                                                                                                                                              >  1:34700/1‑300 (MQ=255)                                                            cttTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGTATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCa                                                                                                                                                                                                                              >  1:227105/1‑300 (MQ=255)                                                            cttTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgcctgcc                                                                                                                                                                                                                               >  2:34293/1‑299 (MQ=255)                                                                                  aattgatgaGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGTCGGCACACTGGCTGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGTTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAac                                                                                                                                                                                                        <  2:476189/297‑1 (MQ=255)                                                                                         gagaTAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTa                                                                                                                                                                                                 >  1:48120/1‑300 (MQ=255)                                                                                          agaTAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTaa                                                                                                                                                                                                >  1:292365/1‑300 (MQ=255)                                                                                          agaTAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGAAGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCATGCCAGATAGCCTACTCACTTTTCAACACCCATaa                                                                                                                                                                                                >  1:214329/1‑300 (MQ=255)                                                                                           gaTAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAat                                                                                                                                                                                               >  1:293730/1‑300 (MQ=255)                                                                                                  ggTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAGCCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAatcacatc                                                                                                                                                                                         >  1:2709/1‑299 (MQ=255)                                                                                                           tgatgaAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATc                                                                                                                                                                                <  1:449257/299‑1 (MQ=255)                                                                                                                  aaTTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCAcc                                                                                                                                                                        <  1:155086/300‑1 (MQ=255)                                                                                                                    ttATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGTTCGTATCCGCTCACCa                                                                                                                                                                       >  2:65118/1‑299 (MQ=255)                                                                                                                       tCATTCTGGATGCCTGCCAGGTTGGCGGCACACTGGCGTAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCATGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGCTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCACTCGTATCCGCTCACCAGACt                                                                                                                                                                   <  2:483034/300‑1 (MQ=255)                                                                                                                                                       cTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATttcattca                                                                                                                                 >  2:315964/1‑300 (MQ=255)                                                                                                                                                           gaaaccctgggtggtgcncgaACAACGATATTACTGNCTGTCGACGACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCACCCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGCNATAAGCTATttcattc                                                                                                                                  <  1:464044/281‑1 (MQ=255)                                                                                                                                                                 aCCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGc                                                                                                                       <  1:323919/300‑1 (MQ=255)                                                                                                                                                                        tGGCGAGAGCAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGTGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGAtt                                                                                                                  <  2:512169/298‑1 (MQ=255)                                                                                                                                                                                      gtatTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCAGACCTTACAGGATAAATTTGACCTGCGGATCGTGCAGGGGCATGGCTCTCATCC‑ACGAGTATTGCGAGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTACCCCGTTCAGCTGCACCCAATACGGTTGCCTGCCAGGTCGCATACTCACTTTTCAACACCACTAACCGCATCGCTCGTATCCGCTCACCAGACTACGTGAGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCAc                                                                                                  >  2:283933/2‑300 (MQ=255)                                                                                                                                                                                      atatTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCAc                                                                                                  >  1:93685/1‑300 (MQ=255)                                                                                                                                                                                      atatTACTGT‑TGTCGA‑TACAAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCa                                                                                                   >  2:130390/1‑299 (MQ=255)                                                                                                                                                                                         ttACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCag                                                                                               <  1:4767/300‑1 (MQ=255)                                                                                                                                                                                          tACTAT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATGTGACCTGCTGGTCGTGCAGGGGCATGGCTCGCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTAGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTGGCCTGCCAGGTAGTCTACTCACTTTTCAACACCCCTAATCGCACCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATGCAGATGCTGTGCCGATTGa                                                                                                                <  2:134828/282‑1 (MQ=255)                                                                                                                                                                                              gt‑tgtCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAg                                                                                          <  1:555248/300‑1 (MQ=255)                                                                                                                                                                                                 tgtCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACNAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGtt                                                                                        >  2:402222/1‑300 (MQ=255)                                                                                                                                                                                                 tgtCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGNGCCGACGACGCCGATATGCTGGTTGCTGTAACNAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGTGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCAGATTGATCATCTGATTGCACCAGAGCAGtt                                                                                        >  2:425738/1‑300 (MQ=255)                                                                                                                                                                                                  gtCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGtt                                                                                        >  1:17569/1‑299 (MQ=255)                                                                                                                                                                                                              cGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGataa                                                                            >  1:257238/1‑300 (MQ=255)                                                                                                                                                                                                              cGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTCTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCAACAGAGCAGTTGGTTATCGataa                                                                            >  2:40327/1‑300 (MQ=255)                                                                                                                                                                                                                  gAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATAtt                                                                        >  1:307273/1‑300 (MQ=255)                                                                                                                                                                                                                         ttcgtaCCTTACAGGATAAATTTGACCTGAGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGAc                                                                 <  2:359572/295‑1 (MQ=255)                                                                                                                                                                                                                               ccTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGAtt                                                            >  1:18126/1‑299 (MQ=255)                                                                                                                                                                                                                                 ttACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAg                                                         <  1:313127/300‑1 (MQ=255)                                                                                                                                                                                                                                 ttACAGGATAAATTTGACCTGCGGGGCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAg                                                         <  2:391838/300‑1 (MQ=255)                                                                                                                                                                                                                                      ggATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGTTCGTATCCGCCCACCAGACTACGTGTGCGATGTCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATTTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACAGAATGAGTGAGTATcc                                                    >  2:260219/1‑300 (MQ=255)                                                                                                                                                                                                                                                 aCCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGc                                         <  1:453465/300‑1 (MQ=255)                                                                                                                                                                                                                                                    tGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAg                                       <  1:171160/299‑1 (MQ=255)                                                                                                                                                                                                                                                            gTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGCTGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTCATc                                                                                 >  2:35381/1‑249 (MQ=255)                                                                                                                                                                                                                                                            gTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGAAc                              >  1:130248/1‑300 (MQ=255)                                                                                                                                                                                                                                                            gTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCANCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGAAc                              >  1:406523/1‑300 (MQ=255)                                                                                                                                                                                                                                                            gTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATAAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTCATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCAG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGAAc                              >  2:486362/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         ggtatcATCC‑ACGGGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGACCTTCGCTGAGGGTa                 >  1:485521/5‑300 (MQ=255)                                                                                                                                                                                                                                                                         gCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGCTCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGAACTTAGCTGAGGGTa                 >  2:451452/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGAACTTCGCTGAGGGTa                 >  1:435286/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gCTCTCATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGAATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGAACTTCGCTGAGGGTa                 >  1:435283/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             tcATCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGAACTTCGCTGAGGGTAAAGt             <  1:387758/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               aTCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGAGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAg                                       >  2:45825/1‑272 (MQ=255)                                                                                                                                                                                                                                                                               aTCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGAGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAg                                       <  1:45825/272‑1 (MQ=255)                                                                                                                                                                                                                                                                               aTCC‑ACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCGGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTACTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGAACTTCGCTGAGGGTAAAGt             <  2:79837/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                     cgcgTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGAACTTCGCTGAGGGTAAAGTCAGCCt       >  1:434516/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                         tATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGAACTTCGCTGAGGGTAAAGTCAGCCTGGCtg  <  2:292365/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         tATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGAACTTCGCTGAGGGTAAAGTCAGCCTGGCtg  <  1:280462/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                    GGAACACCAGAGCAACCGGGTAAACAAAATCTACCTGGTGCCGAAGAGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGT‑TGTCGA‑TACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCC‑ACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCG‑‑ATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCCGGCGCATTGCAGGTGGTGAACTTCGCTGAGGGTAAAGTCAGCCTGGCTG  >  NC_000913/3436410‑3436986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑