Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,316,428	C→T	E367K (GAA→AAA)	nusA ←	transcription termination/antitermination protein NusA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,316,428	0	C	T	100.0%	177.3 / NA	51	E367K (GAA→AAA)	nusA	transcription termination/antitermination protein NusA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/29);  total (22/29)

ATCAATGCCCTGTTCGGCGAGATCTTCCAGCGTACAAACGCCACGGGCGGCCAGTTTGAATGCCAAATCACGATCTACCCCTTCAAGGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTC  >  NC_000913/3316132‑3316722                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                       atcaATGCCCTGTTCGGCTAGATCTTCCAGCGTACAAACGCCACGGGCGGCCAGTTTGAATGCCAAATCACGATCTACCCCTTCAATGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCTAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTttgtc                                                                                                                                                                                                                                                                                                     <  2:32367/300‑1 (MQ=255)                                gTACAAAGGCCATGGGTGGCCGGTTTGGATGTCAAAGCATGGTCTACCCCTGCAAGGTTCAGCAGATAGTCAGCCGGTTGGTTATCACCGATGCTTTCTTCTCTGT‑CCTGTGCAATGGCGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGTGCGGCTCATCAAGGCCTTCGAGGTCCAACAGCTCTTTCAGTGGCACATAGGCCAAGTCTTCCAGCTTCGAGAAGCCTTCTGCTACCCTAACATTCTCGAAGTCTTTGTCAATGTCGATATATTTTTTGGAGGTGTCGacc                                                                                                                                                                                                                                                                      <  2:242715/300‑3 (MQ=255)                                gTACAAACGCCACGGGCGGTCAGTTTGAATGCCAAATCACGATCTACCCCTTCAAGGTTCAGCAGATCGTCAGCCGGGTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTTTCGATGTCGAGATATTTGGTGAAGGTGTCGAt                                                                                                                                                                                                                                                                       <  2:116385/299‑1 (MQ=255)                                gTACAAACGCCACGGGCGGCCAGTTTGAATGCCAAATCACGATCTACCCCTTCAAGGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTNATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATc                                                                                                                                                                                                                                                                      >  2:166818/1‑300 (MQ=255)                                  aCAAACGCCACGGGCGGCCAGTTTGAATGCCAAAGCACGATCTACCCCTTCAAGGTTCAGCAGATCGTCGGCTGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCTAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGAt                                                                                                                                                                                                                                                                       <  2:114999/297‑1 (MQ=255)                                       cGCCACGGGCGGCCAGTTTGAATGCCAAATCACGATCTACCCCTTCAAGGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTNTGCAATGGTGGCCAGTGCATTTTTAGCACGCTNGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCtgcgt                                                                                                                                                                                                                                                               >  2:424387/1‑300 (MQ=255)                                               gcggcCAGTTTGAAGGCCAAATCACGATCTACCCCGTCAAGGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCTAGGCTTTCTCC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTc                                                                                                                                                                                                                                                        <  2:234935/299‑1 (MQ=255)                                                      gTTTGAATGCCAAATCACGATCTACCCCTTCAAGGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGAt                                                                                                                                                                                                                                                >  1:267031/1‑300 (MQ=255)                                                              gCCAAATCACGATCTACCCCTTCAAGGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGcc                                                                                                                                                                                                                                        <  2:532427/300‑1 (MQ=255)                                                                   aTCACGATCTACCCCTTCAAGGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCa                                                                                                                                                                                                                                    >  1:60350/1‑299 (MQ=255)                                                                            tACCCCTTCAAGGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCTAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCaa                                                                                                                                                                                                                           <  2:325144/299‑1 (MQ=255)                                                                            tACCCCTTCAAGGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACANAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTNCTACCATAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCaa                                                                                                                                                                                                                           <  2:418571/299‑1 (MQ=255)                                                                                 cTTCAAGGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTACAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTAGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCATGATGCTTAGCCTGCAGGTCGTCAACGGtca                                                                                                                                                                                                                     >  2:120235/1‑300 (MQ=255)                                                                                            agcagATTGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGTTGGCCAGTGCATTTTTAGCACGCGCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCTAGATATTTGTTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAg                                                                                                                                                                                                           <  2:382195/299‑1 (MQ=255)                                                                                            agcagATCGTCAGCCGGTTTGTTATCACCTAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTNCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGt                                                                                                                                                                                                          <  2:443842/300‑1 (MQ=255)                                                                                             gcagATCGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCTTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGtt                                                                                                                                                                                                         <  1:270458/300‑1 (MQ=255)                                                                                                     tCAGCCGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCTAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCg                                                                                                                                                                                                 <  1:204985/300‑1 (MQ=255)                                                                                                                gTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGa                                                                                                                                                                                      >  1:536191/1‑300 (MQ=255)                                                                                                                                tcttc‑CTTGGCCTGTGCAATGGTGGCCAGTGCATTTTGAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCTGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGTTCAGCTGCGAAGCCAGACGCACGTTc                                                                                                                                                                      <  2:132558/300‑1 (MQ=255)                                                                                                                                       tGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCNATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGAcc                                                                                                                                                                 <  1:108954/299‑1 (MQ=255)                                                                                                                                       tGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGAcc                                                                                                                                                                 <  1:452272/299‑1 (MQ=255)                                                                                                                                       tGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGAc                                                                                                                                                                  <  1:278702/298‑1 (MQ=255)                                                                                                                                              gtgCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTAGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCAAGTTCTGACCGTTACGGc                                                                                                                                                         >  2:248627/1‑300 (MQ=255)                                                                                                                                                                 aTTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGt                                                                                                                                       <  1:370684/299‑1 (MQ=255)                                                                                                                                                                                gcgcAGTGCTTAAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACACACGTTCTGACCGTTAAGGCCAATCGACTGCGACAGGTTACCGGCTTCAAAGGc                                                                                                                       >  2:234490/1‑300 (MQ=255)                                                                                                                                                                                     gTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCACTTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGatat                                                                                                                  >  1:290485/1‑300 (MQ=255)                                                                                                                                                                                     gTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGatat                                                                                                                  >  1:537335/1‑300 (MQ=255)                                                                                                                                                                                        cTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCa                                                                                                               >  1:457626/1‑300 (MQ=255)                                                                                                                                                                                            aaCGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCTAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGgt                                                                                                           <  1:132481/300‑1 (MQ=255)                                                                                                                                                                                            aaCGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATgg                                                                                                            <  1:545822/299‑1 (MQ=255)                                                                                                                                                                                            aaCGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGgt                                                                                                           <  1:508050/300‑1 (MQ=255)                                                                                                                                                                                                    gCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTAt                                                                                                   >  1:563542/1‑300 (MQ=255)                                                                                                                                                                                                       catcaAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt                                                                                                >  1:228119/1‑300 (MQ=255)                                                                                                                                                                                                        atcaAGGCCTTCGATTTCCAACAGCTCTTTCATCTGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGTTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt                                                                                                <  2:535809/299‑1 (MQ=255)                                                                                                                                                                                                        atcaAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCtt                                                                                                <  2:535812/299‑1 (MQ=255)                                                                                                                                                                                                                                             acaTAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGGTATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCAAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAAAGTCTGCCGGTGCCAtt                                                          >  2:239459/1‑300 (MQ=255)                                                                                                                                                                                                                                                      aaTTCTTCCAGCGTTGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATc                                                 <  2:60350/300‑1 (MQ=255)                                                                                                                                                                                                                                                            tcCAGCTTCTAGAAGCCTTCTTCTACCAGAACAGTCGCTAAATCTTTGTCGATTTCTAGATATTTGTGGAAGGTGGCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACAGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAAc                                           <  2:324073/300‑1 (MQ=255)                                                                                                                                                                                                                                                            tcCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGTTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAAc                                           <  1:518336/300‑1 (MQ=255)                                                                                                                                                                                                                                                            tcCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAAc                                           <  2:323851/300‑1 (MQ=255)                                                                                                                                                                                                                                                                gCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTgc                                        >  2:559635/1‑299 (MQ=255)                                                                                                                                                                                                                                                                gCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGACTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAAAGTCTGCCGGTGCCATTGCGTTAATCACGAACTgc                                        >  2:213510/1‑299 (MQ=255)                                                                                                                                                                                                                                                                          gCCTTCTTCTACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTatc                             >  1:220704/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                ttctACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCca                       >  2:335124/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   tACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCca                       >  2:99756/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                   tACCAGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCaaca                     >  1:81960/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                       aGAACAGTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGAc                 <  2:536191/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                             gTCGCGAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCAACAGGACGATATc           >  2:82632/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                gcgAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATa       <  1:424387/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  gAAGTCTTTGTCGATGTCGAGATATTTGGTTAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATAc      <  1:434612/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  gAAGTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATAc      <  1:434592/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     gTCTTTGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTc  >  2:329895/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                       ATCAATGCCCTGTTCGGCGAGATCTTCCAGCGTACAAACGCCACGGGCGGCCAGTTTGAATGCCAAATCACGATCTACCCCTTCAAGGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTC‑CTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCCTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGCTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGGTTACCGGCTTCAACGGCGATATCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTC  >  NC_000913/3316132‑3316722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑