Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,272,090	G→A	G594G (GGC→GGT)	uvrA ←	UvrABC excision nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,272,090	0	G	A	100.0%	191.3 / NA	50	G594G (GGC→GGT)	uvrA	UvrABC excision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/40);  total (10/40)

TGGTCGATATCGATCACTTTATCGAAATGCTCCAGCCCCTGAATATCGCGATACGGTGCTGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCGGTGATGCAGGTAAACAGACCCACCGGCAGCGTCAGCGTCACGTCCTTCAGGTTGTTGCCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCT  >  NC_000913/4271803‑4272373                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                            tGGTCGATGTCGATCACTTTTTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGTTTCGGCGATGGTCGCCCCATTCAACTTTCGTTGGGCAATCGGGAACAGTGTGTCGTGAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTAc                                                                                                                                                                                                                                                                                 <  2:1586/300‑1 (MQ=255) tGGTCGATATCGATCACTTTTTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTTGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTAc                                                                                                                                                                                                                                                                                 <  2:496821/300‑1 (MQ=255) tGGTCGATATCGATCACTTTGTCTAACTGCTCCAGTCCCTGAATATCGCGATACGTTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTTAAACCCCTTTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTAc                                                                                                                                                                                                                                                                                 <  2:1592/300‑1 (MQ=255) tGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTNAACAGACCGACTGGCAGCGTCAGCGTCACGTCNTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTAc                                                                                                                                                                                                                                                                                 <  2:424197/300‑1 (MQ=255)                                        tGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTc                                                                                                                                                                                                                                           <  1:285903/299‑1 (MQ=255)                                                gcgATACGGTGCTTGTTCTTCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTTTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCTGCACCGCTATAATTGCTTCCAGTGGAc                                                                                                                                                                                                                                   <  2:21356/299‑1 (MQ=255)                                                  gATACGGTGCCGGTTCGTCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCt                                                                                                                                                                                                                                 <  2:223636/299‑1 (MQ=255)                                                  gATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCt                                                                                                                                                                                                                                 <  2:497402/299‑1 (MQ=255)                                                   aTACGGTGCCTGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAAGCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCt                                                                                                                                                                                                                                 <  2:222573/298‑1 (MQ=255)                                                   aTACGGTGCCGGTTCTGCGATGGTCGCCCCATTCAACTTGCGTTGGGCAATCGGGAACAGTGTGTCGTTAAGCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCATTGGACCt                                                                                                                                                                                                                                 <  2:223695/298‑1 (MQ=255)                                                     aCGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTccg                                                                                                                                                                                                                             <  1:194834/300‑3 (MQ=255)                                                       ggTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCTGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTTCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTccg                                                                                                                                                                                                                             <  1:194845/298‑3 (MQ=255)                                                         tgccggTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGAc                                                                                                                                                                                                                         <  2:345753/296‑1 (MQ=255)                                                                  gtcgctGGTCGCCCCATTCAACCGTCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCGGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCGGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCAcc                                                                                                                                                                                                                <  2:244330/295‑1 (MQ=255)                                                                  ggCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTTTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCATTGGACCTTCCGCGACCACTTCAcc                                                                                                                                                                                                                <  2:244576/300‑1 (MQ=255)                                                                  ggCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCAcc                                                                                                                                                                                                                <  2:513446/300‑1 (MQ=255)                                                                   gcgaggttCGCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCTTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCAcc                                                                                                                                                                                                                <  2:244313/292‑1 (MQ=255)                                                                            gCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAac                                                                                                                                                                                                       <  2:545985/299‑1 (MQ=255)                                                                               ccATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCt                                                                                                                                                                                                   >  2:186023/1‑300 (MQ=255)                                                                               ccATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCAGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCt                                                                                                                                                                                                   >  1:16331/1‑300 (MQ=255)                                                                                 actCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTTTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACAcc                                                                                                                                                                                                    <  2:51203/295‑1 (MQ=255)                                                                                 aTTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCTGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACAcc                                                                                                                                                                                                    <  1:490588/297‑1 (MQ=255)                                                                                 aTTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACAcc                                                                                                                                                                                                    <  1:215554/297‑1 (MQ=255)                                                                                     aaCTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGcc                                                                                                                                                                                              <  1:546223/299‑1 (MQ=255)                                                                                     aaCTGGCGTTGGGAAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAacac                                                                                                                                                                                                     <  2:262009/292‑1 (MQ=255)                                                                                          gCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGATTCCAGTGGACCTTCCGCGACCATTTCACCGCCTTGAACCCTTGCGCCCGGGcc                                                                                                                                                                                        >  2:72051/1‑300 (MQ=255)                                                                                                ggCAATCGGGAACGGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACATACCGACTGGCAGCGTCAGCGTCACTTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCATTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGt                                                                                                                                                                                   <  2:88567/299‑1 (MQ=255)                                                                                                ggCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGt                                                                                                                                                                                   <  2:158618/299‑1 (MQ=255)                                                                                                              gtgtgtCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGGCCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTATTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTACGGCACCGCCATAATTGCTTCCAGTGGACCTTCAGCGACCACTTCACCGCCGTGAACACCTGTGACCGGGCCAATGTCGATCACATGGTCa                                                                                                                                                                     >  1:140218/1‑299 (MQ=255)                                                                                                              gtgtgtCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCa                                                                                                                                                                     >  1:389475/1‑299 (MQ=255)                                                                                                                 tgtCGTTAATCAGCTTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAgcgg                                                                                                                                                                 <  2:295029/300‑1 (MQ=255)                                                                                                                                              ccTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTccacc                                                                                                                                    >  1:173475/1‑300 (MQ=255)                                                                                                                                                         tgTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGt                                                                                                                         <  2:376595/299‑1 (MQ=255)                                                                                                                                                                cAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTAcc                                                                                                                   >  1:233394/1‑299 (MQ=255)                                                                                                                                                                 aGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTGCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCGCATGTACTATCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCg                                                                                                                  <  2:117800/299‑1 (MQ=255)                                                                                                                                                                  ggTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTCTTACCga                                                                                                                 >  2:501920/1‑299 (MQ=255)                                                                                                                                                                  ggTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTNTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCgag                                                                                                                <  1:402721/300‑1 (MQ=255)                                                                                                                                                                  ggTAAACAGACCGACTGGCAGCGTCAGCGTCAAGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGAATCCGGCACCGCAATAATTGCTTCCAGTGGACCTTCCGCGACCCCTTCACCACCGTGAACACCTGCGCCCGGGACAACGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACaa                                                                                                                                >  2:259260/1‑284 (MQ=255)                                                                                                                                                                                           agcgtcaCGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTAccc                                                                                       <  1:521274/300‑1 (MQ=255)                                                                                                                                                                                                             ttattaccGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATc                                                                      >  1:487735/7‑299 (MQ=255)                                                                                                                                                                                                                           gcCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGc                                                        <  2:475175/299‑1 (MQ=255)                                                                                                                                                                                                                              tGTCAGCTTCAGCACTTTTTCCGTATTCGCCGGAATGCTTTTCTTCGGCATTTCAATCTTGCGTTTACTGCTCAGGTACTGTCCGTTTAACGACTCCTGCACCGCCATAATTGCTTCCAGTGGACCTTCCTCGACCACTTCACCGCCGTGAACACCTGCGCCCTGGCCAATGTCGATCACATGGTCAGCGGCACGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAGCAgg                                                                                 <  2:199236/271‑1 (MQ=255)                                                                                                                                                                                                                                  aGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTACTCCACCACAATCACGGTATTACCGAGATCTCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAg                                                  <  2:140510/298‑1 (MQ=255)                                                                                                                                                                                                                                           aCTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTcc                                       <  2:276511/300‑1 (MQ=255)                                                                                                                                                                                                                                           aCTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTcc                                       <  1:520623/300‑1 (MQ=255)                                                                                                                                                                                                                                                             ggAACGCGTTTCTTCTGCACTTCAATCTGGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCTCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATTGCTCGTCCAGCACGTACATAACGc                       <  2:187358/298‑1 (MQ=255)                                                                                                                                                                                                                                                                     tttCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGcc             <  1:512797/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          tcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCAc        <  1:374880/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 ttCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCt  <  2:551024/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                 ttCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCt  <  1:12241/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                            TGGTCGATATCGATCACTTTATCGAAATGCTCCAGCCCCTGAATATCGCGATACGGTGCTGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCGGTGATGCAGGTAAACAGACCCACCGGCAGCGTCAGCGTCACGTCCTTCAGGTTGTTGCCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCT  >  NC_000913/4271803‑4272373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑