Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,272,009	G→A	N621N (AAC→AAT)	uvrA ←	UvrABC excision nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,272,009	0	G	A	100.0%	192.9 / NA	51	N621N (AAC→AAT)	uvrA	UvrABC excision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/32);  total (19/32)

GCCCGCAAACAGTTCGCGCACAGGCGTAAACACGCCGGTATAGGTCGCCGGGTTAGAACGTGGAGTACGACCAATTGGGCTTTGGTCGATATCGATCACTTTATCGAAATGCTCCAGCCCCTGAATATCGCGATACGGTGCTGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCGGTGATGCAGGTAAACAGACCCACCGGCAGCGTCAGCGTCACGTCCTTCAGGTTGTTGCCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCC  >  NC_000913/4271721‑4272288                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                        gCCCGCAAACAGTTCGCGCACAGACGTAAACACGCCGGTATAGGTCGCCGGGTTAGAACGCGGAGTACGACCAATTGNGCTTTGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTg                                                                                                                                                                                                                                                                               <  1:116516/299‑1 (MQ=255)       aaaCAGTTCGATCACAGACGTAAACACGCCGGTATAGGTCGCCGGGTTAGAACGCGGAGTACGACCAATTGGGCTTTGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACcgcg                                                                                                                                                                                                                                                                                 <  2:236579/291‑1 (MQ=255)                             aaCACGCCGGTATATGTCGCCGGGTTAGAACGCGGAGTACGACCAATTGGGCTTTGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGa                                                                                                                                                                                                                                                  >  1:222573/1‑300 (MQ=255)                             aaCACGCCGGTATATGTCGCCGGGTTAGAACGCGGAGTACGACCAATTGGGCTTTGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTACGGa                                                                                                                                                                                                                                                  >  1:223636/1‑300 (MQ=255)                             aaCACGCCGGTATATGTCGCCGGGTTAGAACGCGGAGTACGACCAATTGGGCTTTGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCAGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCAAGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGa                                                                                                                                                                                                                                                  >  1:223695/1‑300 (MQ=255)                             aaCACGCCGGTATAGGTCGCCGGGTTAGAACGCGGAGTACGACCAATTGGGCTTTGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGa                                                                                                                                                                                                                                                  >  1:497402/1‑300 (MQ=255)                                   ccGGTATAGGTCGCCGGGTTAGAACGCGGAGTACGACCAATTGGGCTTTGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGcc                                                                                                                                                                                                                                            >  1:51203/1‑300 (MQ=255)                                   ccGGTATAGGTCGCCGGGTTAGAACGCGGAGTACGACCAATTGGGCTTTGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCAAGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGcc                                                                                                                                                                                                                                            >  1:158618/1‑300 (MQ=255)                                                  gggTTAGAACGCGGAGTACGACCAATTGGGCTTTGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTttcttc                                                                                                                                                                                                                             >  1:276511/1‑300 (MQ=255)                                                       aGAACGCGGAGTACGACCAATTGGGCTTTGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCAc                                                                                                                                                                                                                        >  2:512607/1‑300 (MQ=255)                                                                 gTACGACCAATTGGGCTTTGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCtt                                                                                                                                                                                                               >  1:551024/1‑299 (MQ=255)                                                                          aTTGGGCTTTGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGtttacc                                                                                                                                                                                                      >  1:187358/1‑296 (MQ=255)                                                                                   tGGTCGATGTCGATCACTTTTTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGTTTCGGCGATGGTCGCCCCATTCAACTTTCGTTGGGCAATCGGGAACAGTGTGTCGTGAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTAc                                                                                                                                                                                            <  2:1586/300‑1 (MQ=255)                                                                                   tGGTCGATATCGATCACTTTTTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTTGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTAc                                                                                                                                                                                            <  2:496821/300‑1 (MQ=255)                                                                                   tGGTCGATATCGATCACTTTGTCTAACTGCTCCAGTCCCTGAATATCGCGATACGTTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTTAAACCCCTTTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTAc                                                                                                                                                                                            <  2:1592/300‑1 (MQ=255)                                                                                   tGGTCGATATCGATCACTTTGTCGAAATGCTCCAGTCCCTGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTNAACAGACCGACTGGCAGCGTCAGCGTCACGTCNTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTAc                                                                                                                                                                                            <  2:424197/300‑1 (MQ=255)                                                                                                                          tGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTc                                                                                                                                                      <  1:285903/299‑1 (MQ=255)                                                                                                                                  gcgATACGGTGCTTGTTCTTCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTTTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCTGCACCGCTATAATTGCTTCCAGTGGAc                                                                                                                                              <  2:21356/299‑1 (MQ=255)                                                                                                                                    gATACGGTGCCGGTTCGTCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCt                                                                                                                                            <  2:223636/299‑1 (MQ=255)                                                                                                                                    gATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCt                                                                                                                                            <  2:497402/299‑1 (MQ=255)                                                                                                                                     aTACGGTGCCTGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAAGCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCt                                                                                                                                            <  2:222573/298‑1 (MQ=255)                                                                                                                                     aTACGGTGCCGGTTCTGCGATGGTCGCCCCATTCAACTTGCGTTGGGCAATCGGGAACAGTGTGTCGTTAAGCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCATTGGACCt                                                                                                                                            <  2:223695/298‑1 (MQ=255)                                                                                                                                       aCGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTccg                                                                                                                                        <  1:194834/300‑3 (MQ=255)                                                                                                                                         ggTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCTGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTTCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTccg                                                                                                                                        <  1:194845/298‑3 (MQ=255)                                                                                                                                           tgccggTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGAc                                                                                                                                    <  2:345753/296‑1 (MQ=255)                                                                                                                                                    gtcgctGGTCGCCCCATTCAACCGTCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCGGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCGGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCAcc                                                                                                                           <  2:244330/295‑1 (MQ=255)                                                                                                                                                    ggCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTTTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCATTGGACCTTCCGCGACCACTTCAcc                                                                                                                           <  2:244576/300‑1 (MQ=255)                                                                                                                                                    ggCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCAcc                                                                                                                           <  2:513446/300‑1 (MQ=255)                                                                                                                                                     gcgaggttCGCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCTTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCAcc                                                                                                                           <  2:244313/292‑1 (MQ=255)                                                                                                                                                              gCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAac                                                                                                                  <  2:545985/299‑1 (MQ=255)                                                                                                                                                                 ccATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCt                                                                                                              >  2:186023/1‑300 (MQ=255)                                                                                                                                                                 ccATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCAGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCt                                                                                                              >  1:16331/1‑300 (MQ=255)                                                                                                                                                                   actCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTTTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACAcc                                                                                                               <  2:51203/295‑1 (MQ=255)                                                                                                                                                                   aTTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCTGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACAcc                                                                                                               <  1:490588/297‑1 (MQ=255)                                                                                                                                                                   aTTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACAcc                                                                                                               <  1:215554/297‑1 (MQ=255)                                                                                                                                                                       aaCTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGcc                                                                                                         <  1:546223/299‑1 (MQ=255)                                                                                                                                                                       aaCTGGCGTTGGGAAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAacac                                                                                                                <  2:262009/292‑1 (MQ=255)                                                                                                                                                                            gCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGATTCCAGTGGACCTTCCGCGACCATTTCACCGCCTTGAACCCTTGCGCCCGGGcc                                                                                                   >  2:72051/1‑300 (MQ=255)                                                                                                                                                                                  ggCAATCGGGAACGGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACATACCGACTGGCAGCGTCAGCGTCACTTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCATTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGt                                                                                              <  2:88567/299‑1 (MQ=255)                                                                                                                                                                                  ggCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGt                                                                                              <  2:158618/299‑1 (MQ=255)                                                                                                                                                                                                gtgtgtCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGGCCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTATTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTACGGCACCGCCATAATTGCTTCCAGTGGACCTTCAGCGACCACTTCACCGCCGTGAACACCTGTGACCGGGCCAATGTCGATCACATGGTCa                                                                                >  1:140218/1‑299 (MQ=255)                                                                                                                                                                                                gtgtgtCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCa                                                                                >  1:389475/1‑299 (MQ=255)                                                                                                                                                                                                   tgtCGTTAATCAGCTTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAgcgg                                                                            <  2:295029/300‑1 (MQ=255)                                                                                                                                                                                                                                ccTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTccacc                                               >  1:173475/1‑300 (MQ=255)                                                                                                                                                                                                                                           tgTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGt                                    <  2:376595/299‑1 (MQ=255)                                                                                                                                                                                                                                                  cAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTAcc                              >  1:233394/1‑299 (MQ=255)                                                                                                                                                                                                                                                   aGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTGCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCGCATGTACTATCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCg                             <  2:117800/299‑1 (MQ=255)                                                                                                                                                                                                                                                    ggTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTCTTACCga                            >  2:501920/1‑299 (MQ=255)                                                                                                                                                                                                                                                    ggTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTNTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCgag                           <  1:402721/300‑1 (MQ=255)                                                                                                                                                                                                                                                    ggTAAACAGACCGACTGGCAGCGTCAGCGTCAAGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGAATCCGGCACCGCAATAATTGCTTCCAGTGGACCTTCCGCGACCCCTTCACCACCGTGAACACCTGCGCCCGGGACAACGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACaa                                           >  2:259260/1‑284 (MQ=255)                                                                                                                                                                                                                                                                             agcgtcaCGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTAccc  <  1:521274/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                        GCCCGCAAACAGTTCGCGCACAGGCGTAAACACGCCGGTATAGGTCGCCGGGTTAGAACGTGGAGTACGACCAATTGGGCTTTGGTCGATATCGATCACTTTATCGAAATGCTCCAGCCCCTGAATATCGCGATACGGTGCTGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCGGTGATGCAGGTAAACAGACCCACCGGCAGCGTCAGCGTCACGTCCTTCAGGTTGTTGCCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCC  >  NC_000913/4271721‑4272288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑