Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,263,231	T→A	T144S (ACC→TCC)	tdcD ←	propionate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,263,231	0	T	A	100.0%	201.0 / NA	59	T144S (ACC→TCC)	tdcD	propionate kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (34/25);  total (34/25)

ATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATT  >  NC_000913/3262944‑3263519                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                 aTCGCCGCTGCGGGTACCCATCATCAAGCCTTCCAGCTGCGTCATTCCCATTTAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCGGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGc                                                                                                                                                                                                                                                                                      <  1:129433/300‑1 (MQ=255)  tCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGcc                                                                                                                                                                                                                                                                                     <  1:284924/300‑1 (MQ=255)                aCCCATCATCAAGCCTTCCAGCGGCGTCATTCCCGTTTAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATACGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAANATAGCGTTGCGACGTGCCGTGGAAACCATAACNGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCTGAGCCATCGTCTGGTGGAAACTGGAATCAAGTACCGCCACCTGAGt                                                                                                                                                                                                                                                                             <  2:425833/294‑1 (MQ=255)                      catcaAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGaaa                                                                                                                                                                                                                                                                 >  1:285069/1‑300 (MQ=255)                      catcaAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCAGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGaaa                                                                                                                                                                                                                                                                 >  1:285196/1‑300 (MQ=255)                             ccTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACTAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATtgc                                                                                                                                                                                                                                                          >  1:296192/1‑300 (MQ=255)                             ccTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATtgc                                                                                                                                                                                                                                                          >  1:296183/1‑300 (MQ=255)                                             ttCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATAc                                                                                                                                                                                                                                          <  1:451420/300‑1 (MQ=255)                                              tCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCNTACACCTAACTCTTCATAATATTTCCACGGCANGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATAcc                                                                                                                                                                                                                                         <  2:425320/300‑1 (MQ=255)                                                     gAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTaaa                                                                                                                                                                                                                                  <  1:502558/300‑1 (MQ=255)                                                       ggTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACTTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAAtt                                                                                                                                                                                                                                >  1:422326/1‑300 (MQ=255)                                                       ggTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAAtt                                                                                                                                                                                                                                >  1:324358/1‑300 (MQ=255)                                                       ggTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCAGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAAATCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCCCTTAAAtt                                                                                                                                                                                                                                >  2:106475/1‑300 (MQ=255)                                                       ggTATCAACACTCTGAATGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCAACGGCAGGCCGTATAAATAAGCTTACGGAGCAATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAAtt                                                                                                                                                                                                                                >  2:233653/1‑300 (MQ=255)                                                               cacTCTGAACGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTAATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAAt                                                                                                                                                                                                                        >  2:186435/1‑300 (MQ=255)                                                                 ctctGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTa                                                                                                                                                                                                                      >  1:40714/1‑300 (MQ=255)                                                                      aCCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAg                                                                                                                                                                                                                 <  1:165004/300‑1 (MQ=255)                                                                                aCCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGTAAACATAGCGGTGCGACGTGCCGTGGAAACCACAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGt                                                                                                                                                                                                       <  2:59708/300‑1 (MQ=255)                                                                                   gcgcAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGNGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTgg                                                                                                                                                                                                     <  1:109767/299‑1 (MQ=255)                                                                                        gATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAAc                                                                                                                                                                                               >  1:332048/1‑300 (MQ=255)                                                                                         aTTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATAAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAAcg                                                                                                                                                                                              <  1:206/300‑1 (MQ=255)                                                                                                  cccctGCCAAGATGCGCCACAACCAGGCCGGCGTCATCTTCCGCCAGATTCCGCAACGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGACACCATAACGGCGTACACCTCACTCTTCATAATATTTCCACGGCAGGTCGTCTAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCAACTGAGTTACGCCCGGACATACTTGCTGCGCCGATTCAATACCACTTAAACTGGCGTAATTATGCAGGGGTGCAAGTGGAGAAACGCGACGGata                                                                                                                                                                                     >  2:134664/5‑300 (MQ=255)                                                                                                     ttGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGAtatta                                                                                                                                                                                  >  1:369547/1‑300 (MQ=255)                                                                                                           aGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTAATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCAACTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCAAGTGGAGAAACGCGACGGATATTATCAAt                                                                                                                                                                             >  2:236263/1‑299 (MQ=255)                                                                                                                cgcCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGAAGGATATTATCAATGACTTc                                                                                                                                                                       >  2:523453/1‑300 (MQ=255)                                                                                                                      aaCCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGt                                                                                                                                                                 >  1:526248/1‑300 (MQ=255)                                                                                                                                gAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTTGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGc                                                                                                                                                        >  1:198472/1‑299 (MQ=255)                                                                                                                                  gTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAgggg                                                                                                                                                                                                              <  2:496868/243‑1 (MQ=255)                                                                                                                                  gTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAgggg                                                                                                                                                                                                              <  2:496884/243‑1 (MQ=255)                                                                                                                                  gTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAgggg                                                                                                                                                                                                              >  1:496868/1‑243 (MQ=255)                                                                                                                                  gTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAgggg                                                                                                                                                                                                              >  1:496884/1‑243 (MQ=255)                                                                                                                                   tCATCTTCCGCCAGATTCGGCAGCGAAGGTACGCACTTGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGTTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGAc                                                                                                                                                    <  2:5614/300‑1 (MQ=255)                                                                                                                                     aTCTTCCGCCAGATTCAGCAGCGAATGTGCACGCTTGTAAACATAGCGGTGCGACGTGCCGTGGAAACCAGAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTTAGTTACGCCCGGAAATAATTGCTGCGCCGATGCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGAc                                                                                                                                                    <  2:6271/298‑1 (MQ=255)                                                                                                                                        ttCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACNCCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGt                                                                                                                                               <  1:106475/300‑1 (MQ=255)                                                                                                                                               aGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAAt                                                                                                                                         <  1:14706/299‑1 (MQ=255)                                                                                                                                                            aaTGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAg                                                                                                                           <  1:233653/300‑1 (MQ=255)                                                                                                                                                             aTGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGc                                                                                                                          <  1:22229/300‑1 (MQ=255)                                                                                                                                                              tgtgCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCGTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGTTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGc                                                                                                                          <  2:150267/299‑1 (MQ=255)                                                                                                                                                                        gggAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGCCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCACGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAGCTATGCAGGGGTGCCAGTGGAGAAACACGACGGATATTATCACTGCCTTCATCGGt                                                                                                                                                                 >  2:205316/1‑250 (MQ=255)                                                                                                                                                                        gggAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCAAGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAACTTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGAATTCATCGGTAATAATGGAGGACTAGGTAAAAATACTaccgca                                                                                                                                >  2:206935/1‑282 (MQ=255)                                                                                                                                                                        gggAAACATAGCGGTGCGACGTGCCGCGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCCCCTGAGTTACGCCAGGCAATAATTGCTGCGCCGATTCAATACCACTTAAATTAGCGTCATTATACAGGGATGCCAGTGGAGAAATGCGACGGATATTCTCAATGACTTAATCGGTAATCACGGCGGAATCGGTCAAAATACTACAGACGTAAGCGATGTGGTGGc                                                                                                               >  2:205280/1‑300 (MQ=255)                                                                                                                                                                           aaaCATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCaa                                                                                                            >  1:442891/1‑300 (MQ=255)                                                                                                                                                                                    ggTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGcc                                                                                                    <  1:459978/299‑1 (MQ=255)                                                                                                                                                                                         gACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCTGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACt                                                                                               <  1:334322/299‑1 (MQ=255)                                                                                                                                                                                            gTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCa                                                                                           >  1:171997/1‑300 (MQ=255)                                                                                                                                                                                                      aaCCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCAAACTGTCATTTAAATTcc                                                                                 >  2:339910/1‑300 (MQ=255)                                                                                                                                                                                                        ccATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGt                                                                               >  1:316157/1‑300 (MQ=255)                                                                                                                                                                                                               ggCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCAAACTGTCATTTAAATTCCGTTTTTCCa                                                                        >  1:341305/1‑300 (MQ=255)                                                                                                                                                                                                               ggCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCAAACTGTCATTTAAATTCCGTTTTTCCa                                                                        >  2:58350/1‑300 (MQ=255)                                                                                                                                                                                                                     cacCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCACTTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCaa                                                                  >  2:89718/1‑300 (MQ=255)                                                                                                                                                                                                                                      tatTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAAt                                                 <  1:101953/300‑1 (MQ=255)                                                                                                                                                                                                                                                  aGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAg                                     <  2:512506/300‑1 (MQ=255)                                                                                                                                                                                                                                                   ggCCGTAGAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCTTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGGAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTGCGTAGc                                    <  1:186961/300‑1 (MQ=255)                                                                                                                                                                                                                                                       gTATAAATAAGCTTCCGGAGCCATCGTCTGGTGTAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAg                                     >  1:541181/1‑295 (MQ=255)                                                                                                                                                                                                                                                       gTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCtg                                  <  1:372361/298‑1 (MQ=255)                                                                                                                                                                                                                                                                  cTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCa                        >  2:74386/1‑297 (MQ=255)                                                                                                                                                                                                                                                                  cTTCCGGAGCCATCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGc                      >  2:339253/1‑299 (MQ=255)                                                                                                                                                                                                                                                                             aTCGTCTGGTGGAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATCAAGGCCACACTGTAATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGGGCCAGCGGTGCTGGctct          >  2:131050/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     gtggAAACTGGAATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCAtt  >  1:391264/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                 ATCGCCACTGCGGGTACCCATCATCAAGCCTTCCAGCGGCGTCATTCCCATTGAGGTATCAACACTCTGACCGTTGCGAACCGCGCAGATTGACGCGCCATTGCCAAGATGCGCCACAACCAGGCCGGAGTCATCTTCCGCCAGATTCAGCAGCGAATGTGCGCGCTGGGAAACATAGCGGTGCGACGTGCCGTGGAAACCATAACGGCGTACACCTAACTCTTCATAATATTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCCACACTGTCATTTAAATTCCGTTTTTCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATT  >  NC_000913/3262944‑3263519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑