BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,031,511	C→T	S117S (AGC→AGT)	pepQ →	Xaa‑Pro dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,031,511	0	C	T	98.7%	251.2 / NA	77	S117S (AGC→AGT)	pepQ	Xaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (33/43); minor base G (0/1); total (33/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTACAGC  >  NC_000913/4031217‑4031805

tCGCGATGCGCTGTCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACNCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAgtctg‑c                                                                                                                                                                                                                                                                                                    <  2:169320/299‑1 (MQ=255)

tCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAgtctg‑c                                                                                                                                                                                                                                                                                                    <  1:428814/299‑1 (MQ=255)

  gcgATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAgtctg‑ctgc                                                                                                                                                                                                                                                                                                 >  1:546437/1‑300 (MQ=255)

    gATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCTGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAATTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAgtctg‑ctgcct                                                                                                                                                                                                                                                                                               <  2:135971/300‑1 (MQ=255)

    gATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAgtctg‑ctgcct                                                                                                                                                                                                                                                                                               <  1:296907/300‑1 (MQ=255)

       gcgcTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTAGAAGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCCGCCGAAAGCCGATGGCATTGGTAgtctg‑ctgcctgct                                                                                                                                                                                                                                                                                            >  2:87574/1‑300 (MQ=255)

           tGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTgcgc                                                                                                                                                                                                                                                                                        <  1:457444/300‑1 (MQ=255)

                  cTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCaa                                                                                                                                                                                                                                                                                 >  1:208650/1‑300 (MQ=255)

                  cTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCNGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCaa                                                                                                                                                                                                                                                                                 >  2:438945/1‑300 (MQ=255)

                      aaGCTGGATGCGTTACGTATTCACTCCGGCGAGCTGTTCAATGTGTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTNAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGTTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACGGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGGTGGCATTGGGAGTCTG‑TTGCCTGCTGCGCGCGGCAatat                                                                                                                                                                                                                                                                              <  1:400502/299‑1 (MQ=255)

                       aGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCg                                                                                                                                                                                                                                                                            <  2:50075/300‑1 (MQ=255)

                              tGCGTTACTTATTCACTTCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAGGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTatat                                                                                                                                                                                                                                                                     <  2:184688/300‑1 (MQ=255)

                              tGCGTTACTTATTCACTCCTGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTatat                                                                                                                                                                                                                                                                     <  1:27848/300‑1 (MQ=255)

                              tGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑G‑CAAAGCGTGNGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTatat                                                                                                                                                                                                                                                                     <  2:442922/300‑1 (MQ=255)

                              tGCGTTACTTATTCACTACGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTatat                                                                                                                                                                                                                                                                     <  1:412111/300‑1 (MQ=255)

                               gCGTTACTTATTCACTCCTGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATc                                                                                                                                                                                                                                                                    <  2:481522/300‑1 (MQ=255)

                                  ttACTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGGACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCATGCTGCGCGCGGCAATATCGGTTATATCGGt                                                                                                                                                                                                                                                                 >  2:85158/1‑300 (MQ=255)

                                    aCTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CATATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTcc                                                                                                                                                                                                                                                               >  1:149928/1‑300 (MQ=255)

                                        aTTCACTCCTGTTAGCTGTTCAATGTTTTGCTCTCTGCGATCATCGCCTATCCGTTTAAAGTGAACCCGCAATCG‑CAAAGCGTGGGTGCCAGTAACTCAGGTTCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCCCTGCCGGTTGATTACTCCGGCACAACGTGCGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTGGCTGCCTGCTGCGCGCGGCAATATCGGTTatat                                                                                                                                                                                                                                                                     <  2:460701/300‑1 (MQ=255)

                                        aTTCACTCCTGCGAGCTGTTCAATGTTTTGCTCTCGACGATCATCGCCTATCCGTTTAAAGTGAACCCGCAAT‑TGCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCCCTGCCGGTTGATTACTCCGGCACAACGTGCGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTGGCTGCCTGCTGCGCGCGGCAATATCGGTTatat                                                                                                                                                                                                                                                                     <  2:460694/300‑1 (MQ=255)

                                             cTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCCTTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGa                                                                                                                                                                                                                                                      >  1:496043/1‑300 (MQ=255)

                                             cTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGa                                                                                                                                                                                                                                                      >  2:349826/1‑300 (MQ=255)

                                               ccGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTNGTTGCTGGTGGATGGCGTGAACAAGCCGAAACNGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAAc                                                                                                                                                                                                                                                    >  2:414045/1‑300 (MQ=255)

                                                         gTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCa                                                                                                                                                                                                                                          >  1:367440/1‑300 (MQ=255)

                                                           tCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAAc                                                                                                                                                                                                                                        <  1:380828/300‑1 (MQ=255)

                                                                       tctcGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGTTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGa                                                                                                                                                                                                                              <  1:64144/300‑1 (MQ=255)

                                                                           gacgaTCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGc                                                                                                                                                                                                                           <  1:266454/299‑1 (MQ=255)

                                                                                 catc‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTTCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCAGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAAc                                                                                                                                                                                                                    >  1:128341/1‑300 (MQ=255)

                                                                                   tc‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACAt                                                                                                                                                                                                                  >  1:160209/1‑300 (MQ=255)

                                                                                          tCCGTTGAAAGTGAACCCGCAAT‑G‑CAAAGTGGGGGGGCCTGTAACCCAGGTGCCAAACTGCGGGTTGCTGGTGGAGGGCGTGAACAAGCCGAAACTTTGGTTCTATC‑‑TGTCGGTTGATTGCT‑‑GGCACAATGT‑CGAACCGCGGCC‑GACCTCTTTTTGGACTNAAGATTTGGAAGTGGTCGCGCTGCCGAAAGCCNATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGAAACGTGCTCTGCAAcctgtcttttatacacatctcgcag                                                                                                                                                                                                                <  2:422894/296‑25 (MQ=255)

                                                                                            cGTTTAAAGTGAACCCGCACT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCAGTCCGGTGCCGGAACGTGCGCTGCAAATGGGTATTGAGGCCAGCAACATCAACCCGa                                                                                                                                                                                                          >  1:280266/1‑300 (MQ=255)

                                                                                                 aaaGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGANGTGATCGCGCTGCCGAAAGCCGATGGCATTGGNAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAggg                                                                                                                                                                                                     <  2:412837/300‑1 (MQ=255)

                                                                                                            gCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCNCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTNCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGAc                                                                                                                                                                                            <  2:415652/298‑1 (MQ=255)

                                                                                                            gCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGAc                                                                                                                                                                                            <  2:104545/298‑1 (MQ=255)

                                                                                                                t‑t‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCt                                                                                                                                                                                       >  1:78964/1‑299 (MQ=255)

                                                                                                                  t‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGACGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGc                                                                                                                                                                                     >  2:509580/1‑300 (MQ=255)

                                                                                                                      aaGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑TGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCAtt                                                                                                                                                                                  <  1:361767/300‑1 (MQ=255)

                                                                                                                        gtgTGGGTGCCGGTAACTTAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTTTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTAc                                                                                                                                                                                <  2:477292/298‑1 (MQ=255)

                                                                                                                        gCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTAc                                                                                                                                                                                >  2:455401/1‑300 (MQ=255)

                                                                                                                        gCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTAGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAATTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTAc                                                                                                                                                                                <  2:477304/300‑1 (MQ=255)

                                                                                                                          gTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTa                                                                                                                                                                              <  2:553529/300‑1 (MQ=255)

                                                                                                                             ggTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCg                                                                                                                                                                           <  1:167585/300‑1 (MQ=255)

                                                                                                                                  cGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCt                                                                                                                                                                      >  2:492432/1‑300 (MQ=255)

                                                                                                                                        cTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCaaaa                                                                                                                                                                >  1:203764/1‑300 (MQ=255)

                                                                                                                                         tCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAAc                                                                                                                                                               <  1:92012/300‑1 (MQ=255)

                                                                                                                                             gTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTTACGATGTGGAAGTGCTCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACACGAAAGGGGTTATCGACTAACTGCATTACTATCGCTCCTTCAAAACTGa                                                                                                                                                            >  2:226986/1‑299 (MQ=255)

                                                                                                                                                      tgctggttgctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGaatt                                                                                                                                                   <  1:558537/299‑3 (MQ=255)

                                                                                                                                                         tggttgctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGcc                                                                                                                                               <  2:323233/300‑1 (MQ=255)

                                                                                                                                                         tggttgctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGcc                                                                                                                                               <  1:361030/300‑1 (MQ=255)

                                                                                                                                                              gctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTAt                                                                                                                                          <  1:93406/300‑1 (MQ=255)

                                                                                                                                                               ctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTAt                                                                                                                                          >  1:441116/1‑299 (MQ=255)

                                                                                                                                                                   tggATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑TACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGGGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTg                                                                                                                                     <  2:109636/300‑1 (MQ=255)

                                                                                                                                                                    ggATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCTGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTg                                                                                                                                     <  2:109294/299‑1 (MQ=255)

                                                                                                                                                                        ggCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTCTGCGTGAAgc                                                                                                                                 >  2:483425/1‑299 (MQ=255)

                                                                                                                                                                             gAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGaa                                                                                                                           >  2:459492/1‑300 (MQ=255)

                                                                                                                                                                              aaCAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGaaa                                                                                                                          <  2:491689/300‑1 (MQ=255)

                                                                                                                                                                                                ttCTATT‑‑TGCCGGTTGATTACT‑‑GGCACAACTT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGTAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCAc                                                                                                        <  2:502134/300‑1 (MQ=255)

                                                                                                                                                                                                      c‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACcgcgc                                                                                                   >  2:516826/1‑299 (MQ=255)

                                                                                                                                                                                                                tGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCagaaga                                                                                          >  1:439133/1‑300 (MQ=255)

                                                                                                                                                                                                                 gATTACT‑‑GGCACAACGT‑CGAACCGCTGCCCGACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCagaaga                                                                                          <  2:460706/300‑1 (MQ=255)

                                                                                                                                                                                                                 gATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAgaagaa                                                                                         >  1:344778/1‑300 (MQ=255)

                                                                                                                                                                                                                   ttACT‑‑TGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGc                                                                                       <  2:532658/300‑1 (MQ=255)

                                                                                                                                                                                                                   ttACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGc                                                                                       >  1:378460/1‑300 (MQ=255)

                                                                                                                                                                                                                          ggCACAACGT‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGcgttcc                                                                                  >  1:497959/1‑300 (MQ=255)

                                                                                                                                                                                                                                   t‑CGAACCGCTGCC‑TACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCa                                                                         <  2:524148/300‑1 (MQ=255)

                                                                                                                                                                                                                                   t‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATTTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCa                                                                         <  2:536070/300‑1 (MQ=255)

                                                                                                                                                                                                                                   t‑CGAACCGCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCa                                                                         <  1:155937/300‑1 (MQ=255)

                                                                                                                                                                                                                                           gCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGc                                                                    <  2:470121/298‑1 (MQ=255)

                                                                                                                                                                                                                                           gCTGCC‑GACCTCTTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGa                                                                  >  2:195078/1‑300 (MQ=255)

                                                                                                                                                                                                                                              gCC‑GACCTCTTTTTGGACGGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGTGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGtt                                                               <  2:121439/300‑1 (MQ=255)

                                                                                                                                                                                                                                                       ctTTTTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTNAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCa                                                       >  1:396927/1‑300 (MQ=255)

                                                                                                                                                                                                                                                             ggACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACAtt                                                  >  1:547597/1‑299 (MQ=255)

                                                                                                                                                                                                                                                                     gATGTGGAAGTGATCGCACTGCCGAAGGCTGATGGCATTGTTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGACCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTTAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCATAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTg                                         <  2:213784/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                      aTGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAGCTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTTAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCAGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTg                                         <  2:213719/299‑1 (MQ=255)

                                                                                                                                                                                                                                                                       tgtgGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTTAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTg                                         <  2:213777/298‑1 (MQ=255)

                                                                                                                                                                                                                                                                        gtgGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGAcc                                      >  1:414415/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                        gtgGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGAcc                                      >  2:97757/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                        gtgGAAGTGATCGCGCTGCAGAAAGCCGATGGCCTTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCAGTTATATCGGTCCGGTGCCAGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCCTTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAGTGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCATATCTGAcc                                      >  2:97988/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                              gTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGAcc                                >  1:82904/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                       cTGCCGAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGt                       >  1:227180/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                            gAAAGCCGATGGCATTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATAc                  <  1:296014/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                                         aTTGGTAGTCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTAc     <  2:367440/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                                            ggTAGGCTT‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTACAGc  <  2:83614/300‑1 (MQ=255)

TCGCGATGCGCTGGCGCGCTTCAAGCTGGATGCGTTACTTATTCACTCCGGCGAGCTGTTCAATGTTTT‑‑TCTCGACGATCATC‑CCTATCCGTTTAAAGTGAACCCGCAAT‑T‑CAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATC‑‑TGCCGGTTGATTACT‑‑GGCACAACGT‑CGAACCGCTGCC‑GACCTCTTTCTGGACTGAAGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTG‑CTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTACAGC  >  NC_000913/4031217‑4031805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`