Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,255,906	A→T	A187A (GCT→GCA)	plsB ←	glycerol‑3‑phosphate 1‑O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,255,906	0	A	T	100.0%	212.7 / NA	61	A187A (GCT→GCA)	plsB	glycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/36);  total (25/36)

TTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACAC  >  NC_000913/4255619‑4256196                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                   ttGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGCCTCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCACTCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGAGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCCTACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTa                                                                                                                                                                                                                                                                                        >  1:365536/1‑300 (MQ=255)   gATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCATGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATc                                                                                                                                                                                                                                                                                      <  1:439567/300‑1 (MQ=255)      gCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATccgt                                                                                                                                                                                                                                                                                   >  2:319830/1‑300 (MQ=255)                      ttCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCATTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGAAGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGca                                                                                                                                                                                                                                                                   >  2:211/1‑299 (MQ=255)                             tGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATTGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGgc                                                                                                                                                                                                                                                            <  2:477290/300‑1 (MQ=255)                             tGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGgc                                                                                                                                                                                                                                                            <  1:12525/300‑1 (MQ=255)                                 gCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCa                                                                                                                                                                                                                                                         >  1:89534/1‑299 (MQ=255)                                     aGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGa                                                                                                                                                                                                                                                     <  2:386088/299‑1 (MQ=255)                                      gAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGaaa                                                                                                                                                                                                                                                   >  1:306803/1‑300 (MQ=255)                                      gAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGaa                                                                                                                                                                                                                                                    >  2:533352/1‑299 (MQ=255)                                         tACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTg                                                                                                                                                                                                                                                <  1:49166/300‑1 (MQ=255)                                            ggTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGTAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCATGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACg                                                                                                                                                                                                                                             <  2:243615/300‑1 (MQ=255)                                                 gtcagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCga                                                                                                                                                                                                                                         >  1:140663/1‑299 (MQ=255)                                                 gtcagGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCga                                                                                                                                                                                                                                         >  1:140920/1‑299 (MQ=255)                                                     ggCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGTTGCGCTTTTTAATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGTAGCTTATTAAACAGATCCTGCCGAGCAGGCAGACGTGGGCCCACAGCAGCCGGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGACATATGGCGCGGCGGAACTGACAGCga                                                                                                                                                                                                                                         >  2:192215/1‑295 (MQ=255)                                                        gAATCATCTAGTAAGANAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCNCCATACGGCGCAGCGAAANTGACGGCGAGAAACGca                                                                                                                                                                                                                                 >  1:475959/1‑300 (MQ=255)                                                                        gaAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGAcc                                                                                                                                                                                                                 <  1:453502/300‑1 (MQ=255)                                                                                  gcgaTCTCTTCCATCGACGCAATCGCGTTCTGCTGCGCTTTGTCATTGGAGATTTTTCTGCTGCGCGCTTCATCTTCTACCGCTGTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCATGCAGACGTGGGCCCACAGCAGCCAGACGTTGATGGGCAAAGTGCATACGCGCCACGCGCGCCAATTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAg                                                                                                                                                                                                        <  2:68795/296‑1 (MQ=255)                                                                                    aaTCTCTTCCATCAGCGNAATCGCGTTCTGCTGCGCNTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGNGACCGAGCCACAGTAc                                                                                                                                                                                                     <  1:462474/300‑1 (MQ=255)                                                                                    aaTCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                                                                                                                                     <  1:155366/300‑1 (MQ=255)                                                                                       ctctTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGCCGCGACCGAGCCACAGTAcggc                                                                                                                                                                                                  >  2:518895/1‑297 (MQ=255)                                                                                                cagcgCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAAttt                                                                                                                                                                                         >  1:218806/5‑300 (MQ=255)                                                                                                  gcgCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCt                                                                                                                                                                                       >  1:148331/3‑300 (MQ=255)                                                                                                  gcgCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGACACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCt                                                                                                                                                                                       >  2:384572/3‑300 (MQ=255)                                                                                                                 gctgcGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCa                                                                                                                                                                        <  1:505278/300‑1 (MQ=255)                                                                                                                    gcgcTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATAc                                                                                                                                                                     >  1:487152/1‑300 (MQ=255)                                                                                                                                                gcgcgcTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                                                                                                                                     <  1:230458/240‑1 (MQ=255)                                                                                                                                                gcgcgcTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                                                                                                                                     <  1:229915/240‑1 (MQ=255)                                                                                                                                                gcgcgcTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                                                                                                                                     >  2:230458/1‑240 (MQ=255)                                                                                                                                                gcgcgcTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCAACAGTAc                                                                                                                                                                                                     >  2:229915/1‑240 (MQ=255)                                                                                                                                                         aTCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCAACAGTACGGCGAAAAATTTATGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGccca                                                                                                                                  >  2:311560/1‑297 (MQ=255)                                                                                                                                                                              ggCGCGGGAGGCGAGTAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACACGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGAACGAGCAACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCAGGCGCGCGACCAAACATCAcc                                                                                                             >  2:297771/1‑298 (MQ=255)                                                                                                                                                                              ggCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCAACGa                                                                                                           >  2:299968/1‑300 (MQ=255)                                                                                                                                                                                       ggCGAGCGGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACACGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACTGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTCCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACACCACCGACACTGGCAc                                                                                                  <  2:40027/300‑1 (MQ=255)                                                                                                                                                                                       ggCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCAc                                                                                                  <  1:14323/300‑1 (MQ=255)                                                                                                                                                                                                          aGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGTGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAtt                                                                                <  2:503608/299‑1 (MQ=255)                                                                                                                                                                                                          aGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAtt                                                                                <  1:311560/299‑1 (MQ=255)                                                                                                                                                                                                           gATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAttt                                                                               >  1:420824/1‑299 (MQ=255)                                                                                                                                                                                                            aTCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCAACGACACTGGCACCATCTGCACCTCCAGAttt                                                                               >  2:525995/1‑298 (MQ=255)                                                                                                                                                                                                                      gcaggcagACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACgg                                                                   <  1:386448/300‑1 (MQ=255)                                                                                                                                                                                                                         ggCAGACGTGGGCACACAGCAGCCATACGGTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGNACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGc                                                                <  2:400383/300‑1 (MQ=255)                                                                                                                                                                                                                              aCGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATc                                                           <  1:515832/300‑1 (MQ=255)                                                                                                                                                                                                                               cGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACTCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATc                                                           >  1:338340/1‑299 (MQ=255)                                                                                                                                                                                                                                   ggcccacagCAGCCAGACGTTGACGTGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGAt                                                      <  1:162123/292‑1 (MQ=255)                                                                                                                                                                                                                                   ggcccacagCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGAt                                                      <  1:162412/292‑1 (MQ=255)                                                                                                                                                                                                                                    gcccacagCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCAACGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTAAGGTGCAAATCGAGATa                                                     >  2:185415/8‑300 (MQ=255)                                                                                                                                                                                                                                             aGCCAGACGTTTACGGGCAAAGTACATACGCGCCACACGCGCCATTTTCTGTGCGATAGTTTTATCCGTTCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCAAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTTGCACCATCTACACATCCAGATTTGGGTTGCTACGGTGCAAATCTAGATAGTCGTGGaa                                            <  2:41693/300‑1 (MQ=255)                                                                                                                                                                                                                                             aGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGa                                             <  1:87937/299‑1 (MQ=255)                                                                                                                                                                                                                                             aGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTACTACGGTGCAAATCGAGATAGTCGTGGaa                                            <  1:277459/300‑1 (MQ=255)                                                                                                                                                                                                                                              gCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACNCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAAc                                           <  2:406756/300‑1 (MQ=255)                                                                                                                                                                                                                                              gCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAAc                                           <  1:224155/300‑1 (MQ=255)                                                                                                                                                                                                                                              gCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAAc                                           <  2:325961/300‑1 (MQ=255)                                                                                                                                                                                                                                                        gACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCTAGATAGTCGTGGAACAGCTTAATAg                                 <  2:487152/300‑1 (MQ=255)                                                                                                                                                                                                                                                        gACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAg                                 <  1:319830/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               aCGCGTGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAGCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAg          <  2:20124/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               aCGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAg          <  2:17534/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gcgcgcCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCTAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAgg         <  1:63826/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gcgcgcCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGt        <  1:211/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      ccAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAaca   >  2:341294/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        aGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCTAGATAGTCGTGGAACAACTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAacac  <  2:218806/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                        aGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAacac  <  2:534791/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                   TTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACAC  >  NC_000913/4255619‑4256196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑