Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,272,105	C→T	G589G (GGG→GGA)	uvrA ←	UvrABC excision nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,272,105	0	C	T	100.0%	185.2 / NA	59	G589G (GGG→GGA)	uvrA	UvrABC excision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/38);  total (21/38)

TGAATATCGCGATACGGTGCTGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCGGTGATGCAGGTAAACAGACCCACCGGCAGCGTCAGCGTCACGTCCTTCAGGTTGTTGCCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCT  >  NC_000913/4271842‑4272399                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                       tGAATATCGCGATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTc                                                                                                                                                                                                                                                                     <  1:285903/299‑1 (MQ=255)         gcgATACGGTGCTTGTTCTTCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTTTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCTGCACCGCTATAATTGCTTCCAGTGGAc                                                                                                                                                                                                                                                             <  2:21356/299‑1 (MQ=255)           gATACGGTGCCGGTTCGTCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCt                                                                                                                                                                                                                                                           <  2:223636/299‑1 (MQ=255)           gATACGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCt                                                                                                                                                                                                                                                           <  2:497402/299‑1 (MQ=255)            aTACGGTGCCTGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAAGCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCt                                                                                                                                                                                                                                                           <  2:222573/298‑1 (MQ=255)            aTACGGTGCCGGTTCTGCGATGGTCGCCCCATTCAACTTGCGTTGGGCAATCGGGAACAGTGTGTCGTTAAGCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCATTGGACCt                                                                                                                                                                                                                                                           <  2:223695/298‑1 (MQ=255)              aCGGTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTccg                                                                                                                                                                                                                                                       <  1:194834/300‑3 (MQ=255)                ggTGCCGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCTGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTTCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTccg                                                                                                                                                                                                                                                       <  1:194845/298‑3 (MQ=255)                  tgccggTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGAc                                                                                                                                                                                                                                                   <  2:345753/296‑1 (MQ=255)                           gtcgctGGTCGCCCCATTCAACCGTCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCGGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCGGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCAcc                                                                                                                                                                                                                                          <  2:244330/295‑1 (MQ=255)                           ggCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTTTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCATTGGACCTTCCGCGACCACTTCAcc                                                                                                                                                                                                                                          <  2:244576/300‑1 (MQ=255)                           ggCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCAcc                                                                                                                                                                                                                                          <  2:513446/300‑1 (MQ=255)                            gcgaggttCGCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCTTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCAcc                                                                                                                                                                                                                                          <  2:244313/292‑1 (MQ=255)                                     gCCCCATTCAACTGGCGTTGGGCAATCTGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAac                                                                                                                                                                                                                                 <  2:545985/299‑1 (MQ=255)                                        ccATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCt                                                                                                                                                                                                                             >  2:186023/1‑300 (MQ=255)                                        ccATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCAGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCt                                                                                                                                                                                                                             >  1:16331/1‑300 (MQ=255)                                          actCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTTTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACAcc                                                                                                                                                                                                                              <  2:51203/295‑1 (MQ=255)                                          aTTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCTGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACAcc                                                                                                                                                                                                                              <  1:490588/297‑1 (MQ=255)                                          aTTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACAcc                                                                                                                                                                                                                              <  1:215554/297‑1 (MQ=255)                                              aaCTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGcc                                                                                                                                                                                                                        <  1:546223/299‑1 (MQ=255)                                              aaCTGGCGTTGGGAAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAacac                                                                                                                                                                                                                               <  2:262009/292‑1 (MQ=255)                                                   gCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGATTCCAGTGGACCTTCCGCGACCATTTCACCGCCTTGAACCCTTGCGCCCGGGcc                                                                                                                                                                                                                  >  2:72051/1‑300 (MQ=255)                                                         ggCAATCGGGAACGGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACATACCGACTGGCAGCGTCAGCGTCACTTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCATTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGt                                                                                                                                                                                                             <  2:88567/299‑1 (MQ=255)                                                         ggCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGt                                                                                                                                                                                                             <  2:158618/299‑1 (MQ=255)                                                                       gtgtgtCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGGCCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTATTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTACGGCACCGCCATAATTGCTTCCAGTGGACCTTCAGCGACCACTTCACCGCCGTGAACACCTGTGACCGGGCCAATGTCGATCACATGGTCa                                                                                                                                                                                               >  1:140218/1‑299 (MQ=255)                                                                       gtgtgtCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCa                                                                                                                                                                                               >  1:389475/1‑299 (MQ=255)                                                                          tgtCGTTAATCAGCTTCGATTTACCGGAACCTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAgcgg                                                                                                                                                                                           <  2:295029/300‑1 (MQ=255)                                                                                                       ccTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTccacc                                                                                                                                                              >  1:173475/1‑300 (MQ=255)                                                                                                                  tgTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGt                                                                                                                                                   <  2:376595/299‑1 (MQ=255)                                                                                                                         cAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTAcc                                                                                                                                             >  1:233394/1‑299 (MQ=255)                                                                                                                          aGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTGCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCGCATGTACTATCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCg                                                                                                                                            <  2:117800/299‑1 (MQ=255)                                                                                                                           ggTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTCTTACCga                                                                                                                                           >  2:501920/1‑299 (MQ=255)                                                                                                                           ggTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTNTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCgag                                                                                                                                          <  1:402721/300‑1 (MQ=255)                                                                                                                           ggTAAACAGACCGACTGGCAGCGTCAGCGTCAAGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGAATCCGGCACCGCAATAATTGCTTCCAGTGGACCTTCCGCGACCCCTTCACCACCGTGAACACCTGCGCCCGGGACAACGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACaa                                                                                                                                                          >  2:259260/1‑284 (MQ=255)                                                                                                                                                    agcgtcaCGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTAccc                                                                                                                 <  1:521274/300‑1 (MQ=255)                                                                                                                                                                      ttattaccGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATc                                                                                                >  1:487735/7‑299 (MQ=255)                                                                                                                                                                                    gcCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGc                                                                                  <  2:475175/299‑1 (MQ=255)                                                                                                                                                                                       tGTCAGCTTCAGCACTTTTTCCGTATTCGCCGGAATGCTTTTCTTCGGCATTTCAATCTTGCGTTTACTGCTCAGGTACTGTCCGTTTAACGACTCCTGCACCGCCATAATTGCTTCCAGTGGACCTTCCTCGACCACTTCACCGCCGTGAACACCTGCGCCCTGGCCAATGTCGATCACATGGTCAGCGGCACGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAGCAgg                                                                                                           <  2:199236/271‑1 (MQ=255)                                                                                                                                                                                           aGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTACTCCACCACAATCACGGTATTACCGAGATCTCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAg                                                                            <  2:140510/298‑1 (MQ=255)                                                                                                                                                                                                    aCTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTcc                                                                 <  2:276511/300‑1 (MQ=255)                                                                                                                                                                                                    aCTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTcc                                                                 <  1:520623/300‑1 (MQ=255)                                                                                                                                                                                                                      ggAACGCGTTTCTTCTGCACTTCAATCTGGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCTCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATTGCTCGTCCAGCACGTACATAACGc                                                 <  2:187358/298‑1 (MQ=255)                                                                                                                                                                                                                              tttCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGcc                                       <  1:512797/300‑1 (MQ=255)                                                                                                                                                                                                                                   tcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCAc                                  <  1:374880/300‑1 (MQ=255)                                                                                                                                                                                                                                          ttCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCt                            <  1:12241/299‑1 (MQ=255)                                                                                                                                                                                                                                          ttCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCt                            <  2:551024/299‑1 (MQ=255)                                                                                                                                                                                                                                                          ccGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACACAACGCCAACCAGGCCCGCACCAATTTGGCTCGCAAGACGGATa           >  2:41337/1‑300 (MQ=255)                                                                                                                                                                                                                                                             cTCATGTACTGTCCTGTTAACGACTCCGGCACCGCCATAATTGCTTCCATTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGc        >  1:95071/1‑300 (MQ=255)                                                                                                                                                                                                                                                             cTCATGTACTGTCCTGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGc        >  1:95132/1‑300 (MQ=255)                                                                                                                                                                                                                                                             cTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAATCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATAc          >  1:56277/1‑298 (MQ=255)                                                                                                                                                                                                                                                             cTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGNAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGc        >  1:109169/1‑300 (MQ=255)                                                                                                                                                                                                                                                             cTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGc        >  1:170657/1‑300 (MQ=255)                                                                                                                                                                                                                                                             cTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCACGCACCAATCTGGCTCGCCAGACGGATACGc        >  1:6495/1‑300 (MQ=255)                                                                                                                                                                                                                                                               cATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGACAGATGGCTCGTCCAGCACGTACATAACGCCAAACAGGCACGCACCAATCTGGCTCGCCAGAAGGATACGc        >  2:153930/1‑298 (MQ=255)                                                                                                                                                                                                                                                                aTGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCtg      <  1:344689/299‑1 (MQ=255)                                                                                                                                                                                                                                                                  gTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATTGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCACGCACCAA‑CTGGCTCGCAAGACGGCTACgatgggct  >  2:104210/1‑293 (MQ=255)                                                                                                                                                                                                                                                                  gTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCtgggc   >  1:49427/1‑297 (MQ=255)                                                                                                                                                                                                                                                                  gTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCACGCACCAATCTGGCTCGCCAGACGGATACGCtgggc   >  1:49175/1‑297 (MQ=255)                                                                                                                                                                                                                                                                   tACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCt  <  1:446264/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                       TGAATATCGCGATACGGTGCTGGTTCGGCGATGGTCGCCCCATTCAACTGGCGTTGGGCAATCGGGAACAGTGTGTCGTTAATCAGCGTCGATTTACCGGAACCTGAAACCCCGGTGATGCAGGTAAACAGACCCACCGGCAGCGTCAGCGTCACGTCCTTCAGGTTGTTGCCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCT  >  NC_000913/4271842‑4272399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑