Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,254,865	T→C	A534A (GCA→GCG)	plsB ←	glycerol‑3‑phosphate 1‑O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,254,865	0	T	C	100.0%	285.0 / NA	77	A534A (GCA→GCG)	plsB	glycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/50);  total (27/50)

ATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAGGGCACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGATGCCAGTAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCAGGTACATGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGATTACGCAGCTTGCTTAAACCGCGCAGCATCTGCGGCAGGCTCTCTTTCTCTTTCGTCGCGCCGCGCAGTTCTTTGG  >  NC_000913/4254569‑4255160                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                        aTGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGcc                                                                                                                                                                                                                                                                                                      <  1:132727/300‑1 (MQ=255)   gtgGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATTCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGcc                                                                                                                                                                                                                                                                                                      <  1:132973/298‑1 (MQ=255)              ggttGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCTGCAGAATGATGATGTCGCCGATTGTGTCTTTCCCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTAc                                                                                                                                                                                                                                                                                         <  2:153954/297‑1 (MQ=255)              ggttGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTAc                                                                                                                                                                                                                                                                                         <  1:434969/297‑1 (MQ=255)              gggTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTAc                                                                                                                                                                                                                                                                                         <  1:27382/300‑1 (MQ=255)              gggTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTAc                                                                                                                                                                                                                                                                                         <  2:133737/300‑1 (MQ=255)                 tGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGc                                                                                                                                                                                                                                                                                      <  1:47968/300‑1 (MQ=255)                   aCGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGcac                                                                                                                                                                                                                                                                                    <  2:553046/300‑1 (MQ=255)                   aCGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGcac                                                                                                                                                                                                                                                                                    <  1:296872/300‑1 (MQ=255)                   aCGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTAGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGcac                                                                                                                                                                                                                                                                                    <  1:314871/300‑1 (MQ=255)                   aCGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCACGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCATAAGCGCAGTACAGcac                                                                                                                                                                                                                                                                                    <  2:37104/300‑1 (MQ=255)                   aCGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCNCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGcac                                                                                                                                                                                                                                                                                    <  1:122721/300‑1 (MQ=255)                      aTTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAgg                                                                                                                                                                                                                                                                                 <  1:11991/300‑1 (MQ=255)                                  aGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGttt                                                                                                                                                                                                                                                                     >  2:450806/1‑300 (MQ=255)                                  aGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGttt                                                                                                                                                                                                                                                                     >  1:216137/1‑300 (MQ=255)                                            gcaCCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTCGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGACGCGCAGTACAGCACAGGTTCATGGCGCTTGCCGCGCCt                                                                                                                                                                                                                                                            >  2:155085/1‑299 (MQ=255)                                                 aaCATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGNTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCgtt                                                                                                                                                                                                                                                       >  1:115239/1‑299 (MQ=255)                                                     tatGCTCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTa                                                                                                                                                                                                                                                   <  2:210027/299‑1 (MQ=255)                                                          gcAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCg                                                                                                                                                                                                                                              <  1:278739/299‑1 (MQ=255)                                                               gttgttGCGATAGTAGNTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCANGGCGTTTGCCGCGCCTGCNTTGTTAATGCGTACCAt                                                                                                                                                                                                                                        >  1:460573/1‑300 (MQ=255)                                                                    tgCGATAGTAGTTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGAt                                                                                                                                                                                                                                   <  1:126274/300‑1 (MQ=255)                                                                      cGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAATGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATc                                                                                                                                                                                                                                  >  1:297283/1‑299 (MQ=255)                                                                           gtagGTCATCCGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGgcagc                                                                                                                                                                                                                            >  1:31251/1‑300 (MQ=255)                                                                           gtagGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGgcagc                                                                                                                                                                                                                            >  1:31471/1‑300 (MQ=255)                                                                               gTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCACACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAat                                                                                                                                                                                                                         >  2:114524/1‑299 (MQ=255)                                                                               gTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCAAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCGGATCGGCAGCAata                                                                                                                                                                                                                        >  2:39815/1‑300 (MQ=255)                                                                                 catcaGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAAtatt                                                                                                                                                                                                                      >  1:201650/1‑300 (MQ=255)                                                                                    caGCACCGCTTGCTCGNGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTNGTTAATGCGTACCATCAGNTCGGCAGCAATattatt                                                                                                                                                                                                                   >  1:475269/1‑300 (MQ=255)                                                                                     aGCACCGCTTGCTCGCGTGGCAGAATGATGATGTCGCCGATTGTGTCTTTCCCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCCTGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCTGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTATAGCACAGGTTCATGGCGTTTGCCGCGCCGGCGTTGTTAATGCGTACCAGCAGATCGGCAGCAATATTATTg                                                                                                                                                                                                                  <  2:31251/300‑1 (MQ=255)                                                                                     aGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTg                                                                                                                                                                                                                  <  2:31471/300‑1 (MQ=255)                                                                                                 tCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGt                                                                                                                                                                                                       <  1:210051/299‑1 (MQ=255)                                                                                                     gcgGCAGACTGATGATGTCGCCGATTTTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGTTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAAcc                                                                                                                                                                                                  <  2:453305/300‑1 (MQ=255)                                                                                                     gcgGCAGAATGGTGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCTTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAAcc                                                                                                                                                                                                  <  2:532011/300‑1 (MQ=255)                                                                                                        gCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCAt                                                                                                                                                                                                >  1:276801/1‑299 (MQ=255)                                                                                                          aGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑TAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGc                                                                                                                                                                                              <  1:526535/299‑1 (MQ=255)                                                                                                          aGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGc                                                                                                                                                                                              <  1:526530/299‑1 (MQ=255)                                                                                                               gaggatgTCACCGATTTTGTCTTCCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTTGCGCTGGCTGAATGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACg                                                                                                                                                                                        <  2:310823/297‑1 (MQ=255)                                                                                                                            aTTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGTTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAGGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATg                                                                                                                                                                           <  1:363227/300‑1 (MQ=255)                                                                                                                            aTTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATg                                                                                                                                                                           <  1:363228/300‑1 (MQ=255)                                                                                                                                                        tcagtggCAGCGCTTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑TAGTTGCTCGGTTAACGGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCATAAGCACAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTa                                                                                                                                               <  2:1594/294‑1 (MQ=255)                                                                                                                                                        tCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTa                                                                                                                                               <  1:50766/300‑1 (MQ=255)                                                                                                                                                          aTTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGTTAGAGTCCGTGGAGTAAGGTACGTTGCGNATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTAc                                                                                                                                              <  1:403685/299‑1 (MQ=255)                                                                                                                                                          aTTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGNATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTAc                                                                                                                                              <  1:116855/299‑1 (MQ=255)                                                                                                                                                             tGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGc                                                                                                                                          <  1:539721/300‑1 (MQ=255)                                                                                                                                                                 gcgcgcGATCGATAAGCTCGCTAGCGCTGGCTGAAGGAACGATAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGCGTGAGCGATGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTCCCATCAGATCGGCAGCAATATTATTGAACGTCGGCGTTAACCATGACGGACGCACCGCTTAGATGGGACCGATAGATTAACGCCAGTCTGGTAcgt                                                                                                                                            >  2:292169/1‑292 (MQ=255)                                                                                                                                                                       gATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAaggt                                                                                                                                <  1:263831/300‑1 (MQ=255)                                                                                                                                                                            aTAAGCTCGCTGGCGCTGGCTGAATGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGtc                                                                                                                           <  1:215838/300‑1 (MQ=255)                                                                                                                                                                            aTAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGTTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGtc                                                                                                                           <  1:215678/300‑1 (MQ=255)                                                                                                                                                                            aTAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACGGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGtc                                                                                                                           <  1:559269/300‑1 (MQ=255)                                                                                                                                                                            aTAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGtc                                                                                                                           <  1:215797/300‑1 (MQ=255)                                                                                                                                                                                            tggctgAAGGAACGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGtt                                                                                                           <  1:439882/300‑1 (MQ=255)                                                                                                                                                                                             ggctgAAGGAATGGTAGAGTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTc                                                                                                          >  2:48099/1‑300 (MQ=255)                                                                                                                                                                                                              agTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGCTAACCATGCCGGACGCACCGCTTCGATGGGGTCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAGGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGAAGTaa                                                                                          >  2:50981/1‑299 (MQ=255)                                                                                                                                                                                                               gTCCGTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTCACCATGCAGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGATACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAAcc                                                                                        >  2:5123/1‑300 (MQ=255)                                                                                                                                                                                                                tccttGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAACAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAAccc                                                                                       <  2:55727/296‑1 (MQ=255)                                                                                                                                                                                                                   gTGGAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGa                                                                                    >  1:231311/1‑300 (MQ=255)                                                                                                                                                                                                                   gTGGAGTAAGGTACGTTACGCATCAGATCCAGGTAGCAGGT‑GAGTTACTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCNGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTCGACGTAAccc                                                                                       <  2:406187/297‑1 (MQ=255)                                                                                                                                                                                                                     ggAGTAAGGTACGTTGCGCATCAGATCCAGTTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGAcc                                                                                  <  1:101692/300‑1 (MQ=255)                                                                                                                                                                                                                     ggAGTAAGGTACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGAcc                                                                                  <  1:101741/300‑1 (MQ=255)                                                                                                                                                                                                                        gtaaagtacattGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACACCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGTGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGAACGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGCCGTAACCCTGACCga                                                                                >  2:26492/11‑299 (MQ=255)                                                                                                                                                                                                                          aaggtaCGTTGCGCATCAGATCCAGTTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAATCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGAt                                                                             <  2:21763/295‑1 (MQ=255)                                                                                                                                                                                                                          aaggtaCGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGGGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTCCCATCAGATCGGAAGCAATATTATTGACCGTCGGCGTCAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCCGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGAACGAGAt                                                                             >  2:302249/6‑300 (MQ=255)                                                                                                                                                                                                                          aaggtaCGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGAt                                                                             <  1:481575/295‑1 (MQ=255)                                                                                                                                                                                                                            ggtaCGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGATTa                                                                           >  1:284653/4‑300 (MQ=255)                                                                                                                                                                                                                               aCGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCATAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGATTACGc                                                                        <  1:361212/300‑1 (MQ=255)                                                                                                                                                                                                                               aCGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGAACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGATTACGc                                                                        <  1:235653/300‑1 (MQ=255)                                                                                                                                                                                                                                    gcgcATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCATGCGTTGTTAATGCGTACCATCAGATCGGCAGCAACATTATTAACCGTCGACNTTAACCATGCCGGACGCACCGCTTAGACGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGCAGTTGACGTAACCCTGACCGAGACTAAGCAgctt                                                                   >  2:170444/1‑300 (MQ=255)                                                                                                                                                                                                                                            gATCCAGGTGGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACTTGACGCCAGAAGCGCATTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCTTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGGTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGATTACGCAGCTTGCTTAAAc                                                           <  2:253954/300‑1 (MQ=255)                                                                                                                                                                                                                                            gATCCAGGTAGCAGTTCGAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCAcgcaaagtcga                                                                                              >  1:188982/1‑256 (MQ=255)                                                                                                                                                                                                                                            gATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGATTACGCAGCTTGCTTAAAc                                                           <  2:529733/300‑1 (MQ=255)                                                                                                                                                                                                                                                 aGGTAGCATTT‑GAGTTGTTCGGTTAGCTGCTCGCGGGTGAGTGAGCGCTGACTTGACGCCATAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGGTCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACTGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGATTACGCAGCTTGCTTa                                                              <  2:100960/292‑1 (MQ=255)                                                                                                                                                                                                                                                       cAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGATTACGCAGCTTGCTTAAACCGCGCAGCATc                                                >  1:140111/1‑300 (MQ=255)                                                                                                                                                                                                                                                              agttgCTCGGTTAACTACTCGCGGGTGAGGGAGCGCTGACGTGACGCCATAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGATTACGCAGCTTGCTTAAAc                                                           <  2:253873/283‑1 (MQ=255)                                                                                                                                                                                                                                                                       gTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGATTACGCAGCTTGCTTAAACCGCGCAGCATCTGCGGCAGGctctct                                 >  1:365295/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 cTGACGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGATTCCGCAGCTTGCTTAACCCGCGCAGCATCTGCGGCAGGCTCTCTTTCTCTTTCGTCGCGCCGCGCAGTTc       >  2:274077/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  tgacgtgacgCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCTAGATTACGCAGCTTGCTTAAACCGCGCAGCATCTGCGGCAGGCTCTCTTTCTCTTTCGTCGCGCCGCGCAGTTc       <  1:63363/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     cGTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCCGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGAACGAGATTACGCAGCTTGCTTAAACCGCGCAGCATCTGCGGCAGGCTCTCTTTCTATTTCGTCGCGCCGCGCAGTTCttt    >  2:29167/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                      gTGACGCCAGAAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCTGGTACGTGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGATTACGCAGCTTGCTTAAACCGCGCAGCATCTGCGGCAGGCTCTCTTTCTCTTTCGTCGCGCCGCGCAGTTCTTTgg  <  1:48099/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                        ATGTGGCGATGCTGGGTGACGATTGCCGCCATCAGCGAAGGCAGCACCAACATATGCGCAATGTTGTTGCGATAGTAGGTCATCAGCACCGCTTGCTCGCGCGGCAGAATGATGATGTCGCCGATTGTGTCTTTCTCGACTTCAAACTTGTTCATTTGCAGCGCGTGATCGATAAGCTCGCTGGCGCTGGCTGAAGGAACGGTAGAGTCCGTGGAGTAGGGCACGTTGCGCATCAGATCCAGGTAGCAGTT‑GAGTTGCTCGGTTAACTGCTCGCGGGTGAGTGAGCGCTGACGTGATGCCAGTAGCGCAGTACAGCACAGGTTCATGGCGTTTGCCGCGCCTGCGTTGTTAATGCGTACCATCAGATCGGCAGCAATATTATTGACCGTCGGCGTTAACCATGCCGGACGCACCGCTTCGATGGGATCGATAGATTCACGCCAGTCAGGTACATGCTGGTTAAGGTAGGTCATCAACGGCATTGGTTCACCGAAGTTGACGTAACCCTGACCGAGATTACGCAGCTTGCTTAAACCGCGCAGCATCTGCGGCAGGCTCTCTTTCTCTTTCGTCGCGCCGCGCAGTTCTTTGG  >  NC_000913/4254569‑4255160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑