Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,255,839	A→G	L210L (TTG→CTG)	plsB ←	glycerol‑3‑phosphate 1‑O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,255,839	0	A	G	100.0%	220.4 / NA	59	L210L (TTG→CTG)	plsB	glycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/32);  total (27/32)

GGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGC  >  NC_000913/4255548‑4256134                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                        ggTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGc                                                                                                                                                                                                                                                                                                 <  1:213899/300‑1 (MQ=255)       aatgCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGcccacag                                                                                                                                                                                                                                                                                           <  1:86094/297‑6 (MQ=255)        aGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGNAATCGCGTTCTGCTGCGCTTTTTCATGGTAGANTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGcccacagc                                                                                                                                                                                                                                                                                          <  2:419669/300‑7 (MQ=255)         ggCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGCATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGCAGAGCAGGCAGACGTGGGcccaaagca                                                                                                                                                                                                                                                                                         >  2:224155/1‑293 (MQ=255)             caTATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATAAGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCa                                                                                                                                                                                                                                                                                     >  2:498372/1‑300 (MQ=255)              atatACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGANGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCa                                                                                                                                                                                                                                                                                     <  2:441023/299‑1 (MQ=255)                  aCCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGt                                                                                                                                                                                                                                                                                <  1:148268/300‑1 (MQ=255)                          aTGACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCACCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGc                                                                                                                                                                                                                                                                        <  2:59421/300‑1 (MQ=255)                            gACCGTCGTGGGCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCaa                                                                                                                                                                                                                                                                      <  1:483730/300‑1 (MQ=255)                                gtcgtGGGCCAGCTGNCGAACGCGCTCGGCGTTANGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCANACGTTGACGGGCAAAg                                                                                                                                                                                                                                                                    <  1:473731/298‑1 (MQ=255)                                     gggCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATAc                                                                                                                                                                                                                                                             <  1:274592/300‑1 (MQ=255)                                      ggCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATAcg                                                                                                                                                                                                                                                            <  1:50026/300‑1 (MQ=255)                                      ggCCAGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATAcg                                                                                                                                                                                                                                                            <  1:50050/300‑1 (MQ=255)                                          aGCTGGCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATAcgcg                                                                                                                                                                                                                                                          <  1:420366/298‑1 (MQ=255)                                               gCGAACGCGCTCGGCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCAcgcg                                                                                                                                                                                                                                                   <  1:235539/300‑1 (MQ=255)                                                         tcggCGTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTc                                                                                                                                                                                                                                         <  1:200582/297‑1 (MQ=255)                                                              gTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTgtgc                                                                                                                                                                                                                                    >  1:17534/1‑297 (MQ=255)                                                              gTTATGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTgtgc                                                                                                                                                                                                                                    >  1:20124/1‑297 (MQ=255)                                                                 aTGGACGTTGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTACATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCACGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAATGCATACGCGACACGCGCGCCAGTTTCTgtacact                                                                                                                                                                                                                                 >  2:278445/1‑294 (MQ=255)                                                                        ttGATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGCCTCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCACTCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGAGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCCTACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTa                                                                                                                                                                                                                          >  1:365536/1‑300 (MQ=255)                                                                          gATGCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCATGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATc                                                                                                                                                                                                                        <  1:439567/300‑1 (MQ=255)                                                                             gCCCTGGTAAAGGCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATccgt                                                                                                                                                                                                                     >  2:319830/1‑300 (MQ=255)                                                                                             ttCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCATTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGAAGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGca                                                                                                                                                                                                     >  2:211/1‑299 (MQ=255)                                                                                                    tGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATTGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGgc                                                                                                                                                                                              <  2:477290/300‑1 (MQ=255)                                                                                                    tGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGgc                                                                                                                                                                                              <  1:12525/300‑1 (MQ=255)                                                                                                        gCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCa                                                                                                                                                                                           >  1:89534/1‑299 (MQ=255)                                                                                                            aGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGTAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGa                                                                                                                                                                                       <  2:386088/299‑1 (MQ=255)                                                                                                             gAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGaaa                                                                                                                                                                                     >  1:306803/1‑300 (MQ=255)                                                                                                             gAATACGGTCAGTCAGGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGaa                                                                                                                                                                                      >  2:533352/1‑299 (MQ=255)                                                                                                                tACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTg                                                                                                                                                                                  <  1:49166/300‑1 (MQ=255)                                                                                                                   ggTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGTAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCATGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACg                                                                                                                                                                               <  2:243615/300‑1 (MQ=255)                                                                                                                        gtcagGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCga                                                                                                                                                                           >  1:140663/1‑299 (MQ=255)                                                                                                                        gtcagGCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCga                                                                                                                                                                           >  1:140920/1‑299 (MQ=255)                                                                                                                            ggCGAATCATCTAGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGTTGCGCTTTTTAATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGTAGCTTATTAAACAGATCCTGCCGAGCAGGCAGACGTGGGCCCACAGCAGCCGGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGACATATGGCGCGGCGGAACTGACAGCga                                                                                                                                                                           >  2:192215/1‑295 (MQ=255)                                                                                                                               gAATCATCTAGTAAGANAAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCNCCATACGGCGCAGCGAAANTGACGGCGAGAAACGca                                                                                                                                                                   >  1:475959/1‑300 (MQ=255)                                                                                                                                               gaAATTCGCCGCAATCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGAcc                                                                                                                                                   <  1:453502/300‑1 (MQ=255)                                                                                                                                                         gcgaTCTCTTCCATCGACGCAATCGCGTTCTGCTGCGCTTTGTCATTGGAGATTTTTCTGCTGCGCGCTTCATCTTCTACCGCTGTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCATGCAGACGTGGGCCCACAGCAGCCAGACGTTGATGGGCAAAGTGCATACGCGCCACGCGCGCCAATTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAg                                                                                                                                          <  2:68795/296‑1 (MQ=255)                                                                                                                                                           aaTCTCTTCCATCAGCGNAATCGCGTTCTGCTGCGCNTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGNGACCGAGCCACAGTAc                                                                                                                                       <  1:462474/300‑1 (MQ=255)                                                                                                                                                           aaTCTCTTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                                                                       <  1:155366/300‑1 (MQ=255)                                                                                                                                                              ctctTCCATCAGCGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGCCGCGACCGAGCCACAGTAcggc                                                                                                                                    >  2:518895/1‑297 (MQ=255)                                                                                                                                                                       cagcgCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAAttt                                                                                                                           >  1:218806/5‑300 (MQ=255)                                                                                                                                                                         gcgCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCt                                                                                                                         >  1:148331/3‑300 (MQ=255)                                                                                                                                                                         gcgCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGACACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCt                                                                                                                         >  2:384572/3‑300 (MQ=255)                                                                                                                                                                                        gctgcGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCa                                                                                                          <  1:505278/300‑1 (MQ=255)                                                                                                                                                                                           gcgcTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATAc                                                                                                       >  1:487152/1‑300 (MQ=255)                                                                                                                                                                                                                       gcgcgcTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                                                                       <  1:230458/240‑1 (MQ=255)                                                                                                                                                                                                                       gcgcgcTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                                                                       >  2:230458/1‑240 (MQ=255)                                                                                                                                                                                                                       gcgcgcTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTAc                                                                                                                                       <  1:229915/240‑1 (MQ=255)                                                                                                                                                                                                                       gcgcgcTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCAACAGTAc                                                                                                                                       >  2:229915/1‑240 (MQ=255)                                                                                                                                                                                                                                aTCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCAACAGTACGGCGAAAAATTTATGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGccca                                                                    >  2:311560/1‑297 (MQ=255)                                                                                                                                                                                                                                                     ggCGCGGGAGGCGAGTAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACACGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGAACGAGCAACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCAGGCGCGCGACCAAACATCAcc                                               >  2:297771/1‑298 (MQ=255)                                                                                                                                                                                                                                                     ggCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCAACGa                                             >  2:299968/1‑300 (MQ=255)                                                                                                                                                                                                                                                              ggCGAGCGGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACACGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACTGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTCCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACACCACCGACACTGGCAc                                    <  2:40027/300‑1 (MQ=255)                                                                                                                                                                                                                                                              ggCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCAc                                    <  1:14323/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGTGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAtt                  <  2:503608/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aGATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAtt                  <  1:311560/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gATCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGAttt                 >  1:420824/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                   aTCCTGACGAGCAGGCAGACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCAACGACACTGGCACCATCTGCACCTCCAGAttt                 >  2:525995/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                             gcaggcagACGTGGGCCCACAGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACgg     <  1:386448/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                ggCAGACGTGGGCACACAGCAGCCATACGGTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGTGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGNACGGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGc  <  2:400383/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                        GGTGGCAAGGCACATATACCAGCTCATGACCGTCGTGGGCCAGCTGGCGAACGCGCTCAGCGTTATGGACGTTGATGCCCTGGTAAAGTCGGTTCCAGGTGAAGCCCAGAATACGGTCAGTCAGGCGAATCATCTCGTAAGAGAAATTCGCCGCAATCTCTTCCATCAGTGCAATCGCGTTCTGCTGCGCTTTTTCATGGGAGATTTTTTTGCTGCGCGCTTCATCTTCTACCGCTTTGGCAATGGCGCGGGAGGCGAGCAGCTTATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGGCGCGCGACCAAACATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGC  >  NC_000913/4255548‑4256134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑