Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,272,222	T→C	A550A (GCA→GCG)	uvrA ←	UvrABC excision nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,272,222	0	T	C	100.0%	185.8 / NA	50	A550A (GCA→GCG)	uvrA	UvrABC excision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/18);  total (32/18)

CCTGAAACCCCGGTGATGCAGGTAAACAGACCCACCGGCAGCGTCAGCGTCACGTCCTTCAGGTTGTTGCCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCGCCGATCTCTTTAAGGATTTTTTCTGCAATCTTCGCC  >  NC_000913/4271944‑4272516                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       ccTGAAACCCCTGTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTccacc                                                                                                                                                                                                                                                                                   >  1:173475/1‑300 (MQ=255)            tgTGATGCAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGt                                                                                                                                                                                                                                                                        <  2:376595/299‑1 (MQ=255)                   cAGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTAcc                                                                                                                                                                                                                                                                  >  1:233394/1‑299 (MQ=255)                    aGGTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTGCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCGCATGTACTATCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCg                                                                                                                                                                                                                                                                 <  2:117800/299‑1 (MQ=255)                     ggTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTCTTACCga                                                                                                                                                                                                                                                                >  2:501920/1‑299 (MQ=255)                     ggTAAACAGACCGACTGGCAGCGTCAGCGTCACGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTNTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCgag                                                                                                                                                                                                                                                               <  1:402721/300‑1 (MQ=255)                     ggTAAACAGACCGACTGGCAGCGTCAGCGTCAAGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGAATCCGGCACCGCAATAATTGCTTCCAGTGGACCTTCCGCGACCCCTTCACCACCGTGAACACCTGCGCCCGGGACAACGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACaa                                                                                                                                                                                                                                                                               >  2:259260/1‑284 (MQ=255)                                              agcgtcaCGTCTTTCAGGTTATTACCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTAccc                                                                                                                                                                                                                                      <  1:521274/300‑1 (MQ=255)                                                                ttattaccGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATc                                                                                                                                                                                                                     >  1:487735/7‑299 (MQ=255)                                                                              gcCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGc                                                                                                                                                                                                       <  2:475175/299‑1 (MQ=255)                                                                                 tGTCAGCTTCAGCACTTTTTCCGTATTCGCCGGAATGCTTTTCTTCGGCATTTCAATCTTGCGTTTACTGCTCAGGTACTGTCCGTTTAACGACTCCTGCACCGCCATAATTGCTTCCAGTGGACCTTCCTCGACCACTTCACCGCCGTGAACACCTGCGCCCTGGCCAATGTCGATCACATGGTCAGCGGCACGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAGCAgg                                                                                                                                                                                                                                <  2:199236/271‑1 (MQ=255)                                                                                     aGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTACTCCACCACAATCACGGTATTACCGAGATCTCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAg                                                                                                                                                                                                 <  2:140510/298‑1 (MQ=255)                                                                                              aCTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTcc                                                                                                                                                                                      <  2:276511/300‑1 (MQ=255)                                                                                              aCTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTcc                                                                                                                                                                                      <  1:520623/300‑1 (MQ=255)                                                                                                                ggAACGCGTTTCTTCTGCACTTCAATCTGGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCTCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATTGCTCGTCCAGCACGTACATAACGc                                                                                                                                                                      <  2:187358/298‑1 (MQ=255)                                                                                                                        tttCTTCGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGcc                                                                                                                                                            <  1:512797/300‑1 (MQ=255)                                                                                                                             tcGGCACTTCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCAc                                                                                                                                                       <  1:374880/300‑1 (MQ=255)                                                                                                                                    ttCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCt                                                                                                                                                 <  1:12241/299‑1 (MQ=255)                                                                                                                                    ttCAATCTTGCGTTTACCGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCt                                                                                                                                                 <  2:551024/299‑1 (MQ=255)                                                                                                                                                    ccGCTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACACAACGCCAACCAGGCCCGCACCAATTTGGCTCGCAAGACGGATa                                                                                                                                >  2:41337/1‑300 (MQ=255)                                                                                                                                                       cTCATGTACTGTCCTGTTAACGACTCCGGCACCGCCATAATTGCTTCCATTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGc                                                                                                                             >  1:95071/1‑300 (MQ=255)                                                                                                                                                       cTCATGTACTGTCCTGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGc                                                                                                                             >  1:95132/1‑300 (MQ=255)                                                                                                                                                       cTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAATCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATAc                                                                                                                               >  1:56277/1‑298 (MQ=255)                                                                                                                                                       cTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGNAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGc                                                                                                                             >  1:109169/1‑300 (MQ=255)                                                                                                                                                       cTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGc                                                                                                                             >  1:170657/1‑300 (MQ=255)                                                                                                                                                       cTCATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCACGCACCAATCTGGCTCGCCAGACGGATACGc                                                                                                                             >  1:6495/1‑300 (MQ=255)                                                                                                                                                         cATGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGACAGATGGCTCGTCCAGCACGTACATAACGCCAAACAGGCACGCACCAATCTGGCTCGCCAGAAGGATACGc                                                                                                                             >  2:153930/1‑298 (MQ=255)                                                                                                                                                          aTGTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCtg                                                                                                                           <  1:344689/299‑1 (MQ=255)                                                                                                                                                            gTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATTGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCACGCACCAA‑CTGGCTCGCAAGACGGCTACgatgggct                                                                                                                       >  2:104210/1‑293 (MQ=255)                                                                                                                                                            gTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCtgggc                                                                                                                        >  1:49427/1‑297 (MQ=255)                                                                                                                                                            gTACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCACGCACCAATCTGGCTCGCCAGACGGATACGCtgggc                                                                                                                        >  1:49175/1‑297 (MQ=255)                                                                                                                                                             tACTGTCCGGTTAACGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCt                                                                                                                       <  1:446264/300‑1 (MQ=255)                                                                                                                                                                      gttaaCGACTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTAGTGCTCCACCACAATCACGGTATTACCGAGATCGAGCAGATGGATAAGCGTACACAACAGGCGCTCGTTATCGCGCTGGTGCAGGCAGATAGATGGCTCGTCCAGCACGTACATAACGCCCACCAGGCCCGCACCAATCCGGCTCGc                                                                                                                                          >  2:345524/4‑272 (MQ=255)                                                                                                                                                                              cTCCGGCACCGCCATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGt                                                                                                      >  1:81570/1‑300 (MQ=255)                                                                                                                                                                                         ccATAATTGCTTCCAGTGGACCTTCCGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTCTGCCGAGc                                                                                           >  1:390055/1‑300 (MQ=255)                                                                                                                                                                                                               ttccgCGACCACTTTACCGCCGTGAACACTTGCGCCCGGGCCAATTGCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGTTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTc                                                                       <  2:16331/294‑1 (MQ=255)                                                                                                                                                                                                                        cACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGt                                                             >  1:177658/1‑299 (MQ=255)                                                                                                                                                                                                                        cACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCACGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGtt                                                            >  1:177457/1‑300 (MQ=255)                                                                                                                                                                                                                          cTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTaa                                                          >  2:461781/1‑300 (MQ=255)                                                                                                                                                                                                                          cTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTaa                                                          >  1:559496/1‑300 (MQ=255)                                                                                                                                                                                                                                 gccgTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGaa                                                   >  1:430265/1‑300 (MQ=255)                                                                                                                                                                                                                                    gtgcacacCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTCTGCCGAGTGGGAAAGCGTCAGGTAATTCAGGCCGAAGTTAACGAGGAAttt                                                >  1:149819/5‑300 (MQ=255)                                                                                                                                                                                                                                    gTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAAttt                                                >  1:151648/1‑300 (MQ=255)                                                                                                                                                                                                                                    gTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTATGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAAttt                                                >  1:149785/1‑300 (MQ=255)                                                                                                                                                                                                                                    gTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCAGGAAAGCGTTTATGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAAttt                                                >  1:149825/1‑300 (MQ=255)                                                                                                                                                                                                                                      gAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTCTGCCGCGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCa                                              >  1:458143/1‑300 (MQ=255)                                                                                                                                                                                                                                                     ggCCAATGTCGATCAAATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACTCANCAGGCGCTCGTTATCGCGCTGGTGCAGGCCGANAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCAGCACTAATCTAGCTCGCAAGACGGATACGCTGGGCTTCACCACCGGGAGGCGTTTCTGCAGAGCGGGAAAGCGTCAGGTAATTCAGGCAGACGTTAACGAGGAATTTTAAACGAtaggagatct                               >  2:417676/1‑291 (MQ=255)                                                                                                                                                                                                                                                          aTGTCGATCACATGGTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCAGACGTTAACGAGGAATTTCAAACGATCGc                                     >  2:468748/1‑289 (MQ=255)                                                                                                                                                                                                                                                                       ggTCAGCGGCGCGAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCCGGTAATTCAGGCCGACGTTCACGAGGAATTTCAACCAATCGCCGATCTCTTTAAGAATTTTTTcc              >  2:209918/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                   gAATCGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCGCGCTGGTGCAGGCCGATAGATGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGGGCTTCACCGCCGGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAAACGATCGCCGATCTCTTTAAGAATTTTTTCCGCAATCTTCGcc  <  1:153930/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       CCTGAAACCCCGGTGATGCAGGTAAACAGACCCACCGGCAGCGTCAGCGTCACGTCCTTCAGGTTGTTGCCGCGTGCGCCTGTCAGCTTCAGCACTTTTTCCGGATTCGCCGGAACGCGTTTCTTCGGCACTTCAATCTTGCGTTTGCCGCTCATGTACTGCCCGGTCAACGACTCCGGCACCGCCATAATCGCTTCCAGCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCGCCGATCTCTTTAAGGATTTTTTCTGCAATCTTCGCC  >  NC_000913/4271944‑4272516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑