Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,264,931	G→A	G206G (GGG→GGA)	dnaB →	replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,264,931	0	G	A	98.9%	312.0 / NA	87	G206G (GGG→GGA)	dnaB	replicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (44/42);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCCGAAGCTGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGATCTGGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCGAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGGGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTT  >  NC_000913/4264645‑4265223                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                     aaaaTACTCCAGGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGATGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGtt                                                                                                                                                                                                                                                                                         <  2:554197/300‑1 (MQ=255)  aaaTACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTa                                                                                                                                                                                                                                                                                        >  2:130596/1‑300 (MQ=255)                  tcTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACaaa                                                                                                                                                                                                                                                                        >  1:496634/2‑300 (MQ=255)                  gCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAGGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACaaa                                                                                                                                                                                                                                                                        >  1:206132/1‑300 (MQ=255)                  gCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACaaa                                                                                                                                                                                                                                                                        >  1:206126/1‑300 (MQ=255)                  gCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTAAACaaa                                                                                                                                                                                                                                                                        >  2:336038/1‑300 (MQ=255)                        attagtaccTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCTTGAGATGGTCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGAc                                                                                                                                                                                                                                                                                    <  2:118129/275‑1 (MQ=255)                        aTCAGTGCCTATGTGGACATCATGCGTGAACGTGCCGTTGTCCGTTAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCATGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCTAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAAc                                                                                                                                                                                                                                                                   <  2:14301/299‑1 (MQ=255)                              gCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCAGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGc                                                                                                                                                                                                                                                            >  2:507422/1‑300 (MQ=255)                                 tATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCtt                                                                                                                                                                                                                                                          >  1:200051/1‑299 (MQ=255)                                    gcgggtaTCGTGCGTGGACGTGTTGTTGTGCGTGAGGTGATTTTGGTTGCGGATGAGATTGTTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGTTGAATCCCGCGTCTTTAAAATTGCCGAAGGTCGTGCAAACAAAGACGAAGGGTCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAGCCGTTGGCTTGCAg                                                                                                                                                                                                                                                      <  2:55965/294‑1 (MQ=255)                                      ggACATCGTGCGTGAACGTGCCGTTGTCCGTTAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGAGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGc                                                                                                                                                                                                                                                     <  1:432980/299‑1 (MQ=255)                                       gACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGcc                                                                                                                                                                                                                                                    >  1:320366/1‑299 (MQ=255)                                       gACATCGTGCGGGAACGTGCCGTTGTCCGGGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCg                                                                                                                                                                                                                                                   <  2:342982/300‑1 (MQ=255)                                         cATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTc                                                                                                                                                                                                                                                 >  1:356290/1‑300 (MQ=255)                                             gtgcgtgAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCANCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGAt                                                                                                                                                                                                                                             >  1:397905/1‑300 (MQ=255)                                              tgcgtgAACGTGCTGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGa                                                                                                                                                                                                                                              <  2:386794/298‑1 (MQ=255)                                              tgcgtgAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACCCCGGTTATGACGATCTCCACAAAAAAACCGCTGGCCTGCAGCCGTCGGAtt                                                                                                                                                                                                                                            >  2:514081/1‑300 (MQ=255)                                                   gAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGatc                                                                                                                                                                                                                                       <  1:42088/300‑1 (MQ=255)                                                    aaCGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGatc                                                                                                                                                                                                                                       >  1:56225/1‑299 (MQ=255)                                                       gTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGatcatc                                                                                                                                                                                                                                    >  1:314810/1‑299 (MQ=255)                                                       gTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCAGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTAAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAACCATCGGAtt                                                                                                                                                                                                                                            >  2:48971/1‑291 (MQ=255)                                                             ttGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTcgccg                                                                                                                                                                                                                             <  1:262890/300‑1 (MQ=255)                                                             ttGTCCGTGAGATGATCTCGGTTGCAAATGAGATTGCTGAAGCAGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTNGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTAAACAAAAAAACCGCTGGCTTGCAGCCGTAGGATTTGATCATCGTcgcc                                                                                                                                                                                                                              >  2:165660/1‑299 (MQ=255)                                                               gTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACNGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTcgccgc                                                                                                                                                                                                                            >  1:108239/1‑299 (MQ=255)                                                                                    gCGAATGAGATTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAaaca                                                                                                                                                                                                      >  2:317659/1‑300 (MQ=255)                                                                                                   gAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAAc                                                                                                                                                                                       <  1:144657/300‑1 (MQ=255)                                                                                                                       ggggCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGAt                                                                                                                                                                   >  2:355762/1‑300 (MQ=255)                                                                                                                                 aGCGAAGGTTTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAGGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGAt                                                                                                                                                         <  2:270158/300‑1 (MQ=255)                                                                                                                                      gggtcggctgGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAAcc                                                                                                                                                    <  2:305816/294‑1 (MQ=255)                                                                                                                                      aGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAAcc                                                                                                                                                    <  1:161512/300‑1 (MQ=255)                                                                                                                                           tgctgGACCTTGCTGAATCCTGCGTCTTTAAAGTTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATTGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTTGCTTGCAGCCGTCGGATTTGATCAGCGTCGCCACGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTAc                                                                                                                                               <  2:96940/300‑1 (MQ=255)                                                                                                                                           tgctgGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCTAAGAACATCGNCGATGTGCTCGACGCAACCGTTGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTAc                                                                                                                                               <  1:102718/300‑1 (MQ=255)                                                                                                                                           tgctgGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGNCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTAc                                                                                                                                               <  1:102930/300‑1 (MQ=255)                                                                                                                                           tgctgGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTAc                                                                                                                                               <  1:124481/300‑1 (MQ=255)                                                                                                                                              tggaccttgCTGAATCCCGCGGCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATATGCTCGACGCAACCGTGGCGCGTATGGAGCAGTTGTTTAAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGGTATGACGAGCTCAACAGAAAAACCGCTTGCTTGCAGTCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTAc                                                                                                                                               <  2:97630/289‑1 (MQ=255)                                                                                                                                                    ttgCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTCTGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTAtcttct                                                                                                                                      >  2:140045/3‑300 (MQ=255)                                                                                                                                                    ttgCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTAtcttc                                                                                                                                       <  2:562616/297‑1 (MQ=255)                                                                                                                                                         gAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCAGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCt                                                                                                                                  >  2:262961/1‑299 (MQ=255)                                                                                                                                                                gcgTCTTTAAAATTGCCGAAAGTCGTGCAAACAAATACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGTTTATGGCGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACGACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGTAGATg                                                                                                                           <  2:190862/299‑1 (MQ=255)                                                                                                                                                                gcgTCTTTAAAATTGCCGAAAGTCGTGCAAACAAATACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGgaga                                                                                                                             <  2:238501/297‑1 (MQ=255)                                                                                                                                                                   tCTTGAAAAGTGCCGAAAGTCGGGCAAACAAAGAAGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGGTACCGTAGTAAACACCGGTTATTACGATCGCAACAAAAAAACCGCTGGCGTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAATATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACGTATCTTCTCGCTGgaga                                                                                                                             <  2:238510/294‑1 (MQ=255)                                                                                                                                                                    cTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGccttc                                                                                                                      >  2:391519/1‑297 (MQ=255)                                                                                                                                                                      ttAAAATTGCCGAAATTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTACTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGTTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGgaga                                                                                                                             <  2:44233/291‑1 (MQ=255)                                                                                                                                                                                   aaGTCGTGCAAACAAAGACGAAGGGCCGAAAAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGCCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTCCTCGCTGGAGATGCCTTCAGAACAGATTatgat                                                                                                       <  2:465854/300‑1 (MQ=255)                                                                                                                                                                                         tgcaaaCAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTtc                                                                                                 <  1:354920/296‑1 (MQ=255)                                                                                                                                                                                            aaaCAAAGACGAAGGGCCGAAGAACGTAGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCATTACCGGAGTAAACACCGGGTATGACGATCTAAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTAGCCGCGCGTCCGTCGATGGGTAAAATAACATTGGCGATGAACCGCGTCGAAAACGCGGCGATGTTGCAGGAGAAACCAGTACGGATCGTTGCGCTGGAGATGCCTTCAGAACAGATTatga                                                                                                        <  2:512360/289‑1 (MQ=255)                                                                                                                                                                                            aaaCAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGTTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCTTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCATTctct                                                                                              <  2:115342/299‑1 (MQ=255)                                                                                                                                                                                            aaaCAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCANCGTCGCCGCGCGTCCGTCGATGGTTAAAACAANATTTGCGATGAACCTCGGCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTctct                                                                                              <  2:420299/299‑1 (MQ=255)                                                                                                                                                                                              aCAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGTCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTgg                                                                                            <  2:391069/300‑1 (MQ=255)                                                                                                                                                                                              aCAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGGCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGTAGATGCCTTCAGAACAGATTATGATGCGTTCTCTgg                                                                                            <  2:535307/300‑1 (MQ=255)                                                                                                                                                                                                aaaGACTAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTTGCTTGCAGCCTTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCg                                                                                          <  2:133887/300‑1 (MQ=255)                                                                                                                                                                                                          ggCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTACGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCACTGTcgc                                                                                >  1:82797/1‑300 (MQ=255)                                                                                                                                                                                                           gCCGAAGAGCATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTAACGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGCACAGATTATGCTGCGTTCTCTGGCGTCGCTGTcgc                                                                                >  2:213110/1‑299 (MQ=255)                                                                                                                                                                                                              gaagaaCATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTAGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGt                                                                            >  2:320337/1‑300 (MQ=255)                                                                                                                                                                                                               aagaaCATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGtt                                                                           >  1:500570/1‑300 (MQ=255)                                                                                                                                                                                                                   aCATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGAcc                                                                       <  2:496634/300‑1 (MQ=255)                                                                                                                                                                                                                              gtgCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCNCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATc                                                            >  2:407350/1‑300 (MQ=255)                                                                                                                                                                                                                              gtgCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATc                                                            >  1:429919/1‑300 (MQ=255)                                                                                                                                                                                                                              gtgCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGATGTCGCGCGTTGACCAGACTAAAATc                                                            >  1:146655/1‑300 (MQ=255)                                                                                                                                                                                                                                gCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATcc                                                           >  1:80156/1‑299 (MQ=255)                                                                                                                                                                                                                                    gACGCAATCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGGGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTAc                                                       <  2:473744/299‑1 (MQ=255)                                                                                                                                                                                                                                    gACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTAc                                                       <  1:266044/299‑1 (MQ=255)                                                                                                                                                                                                                                     aCGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGACCCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCCGAACAGATTATGATGCGTTCTATGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGtccc                                                      >  2:26101/1‑296 (MQ=255)                                                                                                                                                                                                                                         aaCCGTGGCTCCTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGTAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGCCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGc                                                  <  2:451200/299‑1 (MQ=255)                                                                                                                                                                                                                                          aCCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAACCACCGCTGGCTTGCAGCCGTCGGATTTGATCGTCGTCGCCGCGCGTCCGTCGATGGGTAAAACCACATTTGCGATGAACCTCGTCGACAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGANATGccttc                                                                                                                      >  1:121396/1‑227 (MQ=255)                                                                                                                                                                                                                                          aCCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGANATGCCTTCAGACCAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCa                                                 >  1:121616/1‑299 (MQ=255)                                                                                                                                                                                                                                          aCCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGANATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCa                                                 >  1:121445/1‑299 (MQ=255)                                                                                                                                                                                                                                             gTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTc                                             <  1:336038/300‑1 (MQ=255)                                                                                                                                                                                                                                               ggCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGa                                           >  1:388045/1‑300 (MQ=255)                                                                                                                                                                                                                                               ggCGCGTACTGAGCAGTTGTTTCAGCAACCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTAAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCAATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCCTCTCGCTGGAGATGCCTTCCGCACAGACTATGATGCGTTCTCTGGCGTCGCTGTCGTGCATTCACCAGACTAAAATCCATACCGGACAGCCCGa                                           >  2:121215/1‑300 (MQ=255)                                                                                                                                                                                                                                                 cgcgTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTAACGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGAt                                          >  1:171966/1‑299 (MQ=255)                                                                                                                                                                                                                                                    gTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGat                                       >  2:474058/1‑298 (MQ=255)                                                                                                                                                                                                                                                    gTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGat                                       >  1:281296/1‑298 (MQ=255)                                                                                                                                                                                                                                                       ttGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGATGAAg                                   <  2:18677/300‑1 (MQ=255)                                                                                                                                                                                                                                                         gAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTGTGATGCGTTCTCTTGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAACTCGATGATGAAGAc                                 <  1:515027/300‑1 (MQ=255)                                                                                                                                                                                                                                                         gAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGATGAAGAc                                 <  1:515017/300‑1 (MQ=255)                                                                                                                                                                                                                                                              gttgttTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGATGAAGACTGGgc                            >  1:290885/1‑300 (MQ=255)                                                                                                                                                                                                                                                                tgttTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTNCAGGATAAACCAGTACTTATCTTCTAGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGATGAAGaat                                >  2:166442/1‑292 (MQ=255)                                                                                                                                                                                                                                                                          gcCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGATGAAGACTGGGCGCGCATTTcc                  >  1:206166/1‑298 (MQ=255)                                                                                                                                                                                                                                                                           ccACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCATGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGATGAAGACTGGGCGCGCATTTCCGGc               >  1:48871/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    ggCGTTACCGGAGTAANCACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGANGATGAAGACTGGGCGCGCNTTTCCGGCACCATGGGt      >  1:476213/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     gCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGATGAAGACTGGGCGCGCATTTCCGGCACCATGGGTa     >  2:320054/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        ttACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGTAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGATGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAttt  <  2:295775/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        ttACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGATGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAttt  <  1:514529/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        ttACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGATGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAttt  <  2:320366/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        ttACCGGAGGAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCTCCGCTCGTCCTTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGTCGATGTTGCAGGATAAACCAGTACTTATCTCCTCGCTGTAGATGCCTTCAGAACAGATTATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGATGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAt    <  2:206126/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                     AAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGATTGCCGAAGCTGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGATCTGGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCGAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGGGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTT  >  NC_000913/4264645‑4265223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑