Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,096,437	Δ1 bp	coding (594/777 nt)	hisF →	imidazole glycerol phosphate synthase subunit HisF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,096,437	0	A	.	100.0%	279.5 / NA	57	coding (594/777 nt)	hisF	imidazole glycerol phosphate synthase subunit HisF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (38/19);  total (38/19)

CTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAG  >  NC_000913/2096152‑2096734                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                          ctctCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCANTATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTccc                                                                                                                                                                                                                                                                                             >  1:111615/1‑299 (MQ=255) ctctCCTGCGCTGGCAGAACCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCAATCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCANTATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCAACGTccc                                                                                                                                                                                                                                                                                             >  1:112014/1‑299 (MQ=255)        gcgcTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGAtt                                                                                                                                                                                                                                                                                      >  1:268184/1‑299 (MQ=255)                                    gCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAac                                                                                                                                                                                                                                                          <  1:72396/299‑1 (MQ=255)                                            gATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTc                                                                                                                                                                                                                                                  >  1:480605/1‑299 (MQ=255)                                              tCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGaa                                                                                                                                                                                                                                               >  1:4453/1‑300 (MQ=255)                                               cGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAacac                                                                                                                                                                                                                                                        >  1:439488/1‑290 (MQ=255)                                               cGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATANACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAacac                                                                                                                                                                                                                                                        <  2:439488/290‑1 (MQ=255)                                                        gTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGTAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATAATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCTCGGGCACCATGGAACACTTCCTCGAAGCCTTCcgc                                                                                                                                                                                                                                      <  2:162039/299‑1 (MQ=255)                                                        gTGCAGTGTAGTGTGGTCGGTATTGATGCCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGTAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCTCGGGCACCATGGAACACTTCCTCGAAGCCTTCcgc                                                                                                                                                                                                                                      <  2:162030/299‑1 (MQ=255)                                                          gCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGAt                                                                                                                                                                                                                                   >  1:497491/1‑300 (MQ=255)                                                             gtgtATTGTGGTCTGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGcc                                                                                                                                                                                                                                >  1:226684/1‑300 (MQ=255)                                                             gtgtATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTAGAAGCCTTCCGCGATGcc                                                                                                                                                                                                                                >  1:291617/1‑300 (MQ=255)                                                                 aTTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGTGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGACCACTTCCTCGAAGCCTTCCGCAGTGCCGa                                                                                                                                                                                                                              >  2:162493/1‑298 (MQ=255)                                                                        tCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACgg                                                                                                                                                                                                                     <  1:255672/300‑1 (MQ=255)                                                                           gTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCAGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGgcgc                                                                                                                                                                                                                  >  2:303292/1‑300 (MQ=255)                                                                                   aCCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGAACTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCa                                                                                                                                                                                                           >  1:373447/1‑299 (MQ=255)                                                                                            gacgCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTAt                                                                                                                                                                                                 <  1:56214/300‑1 (MQ=255)                                                                                                                 cATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAata                                                                                                                                                                            >  1:383801/1‑300 (MQ=255)                                                                                                                       aaTCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAACCAATATTGGt                                                                                                                                                                       >  2:133767/1‑299 (MQ=255)                                                                                                                         tCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTgca                                                                                                                                                                    >  2:265222/1‑298 (MQ=255)                                                                                                                         tCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTACTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTAGAACAACTGAACAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACAATTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTACTTCTTCCACAACCAAATAATCAATATTGGTGa                                                                                                                                                                     >  2:265138/1‑299 (MQ=255)                                                                                                                                   ggCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCAGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGc                                                                                                                                                            >  1:96259/1‑298 (MQ=255)                                                                                                                                   ggCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTAAGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCAGCTGATTGCCTCCGGTGGCGCGGGCACCATGGCACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGc                                                                                                                                                            >  1:96597/1‑298 (MQ=255)                                                                                                                                      gATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTACGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTAcc                                                                                                                                                       >  1:373329/1‑300 (MQ=255)                                                                                                                                             gCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAac                                                                                                                                                <  2:517780/300‑1 (MQ=255)                                                                                                                                                gCACCCGCGTCATTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAacac                                                                                                                                              <  2:304418/299‑1 (MQ=255)                                                                                                                                                gCACCCGCGTCACTCAATGGGAAACGTTCGACTGTGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCGTCAGCGATGCCGACGGTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAacac                                                                                                                                              <  2:282593/299‑1 (MQ=255)                                                                                                                                                 cACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCaa                                                                                                                                                 >  1:429652/1‑295 (MQ=255)                                                                                                                                                      gcgTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCAGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGAACTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCCAGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGCCGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAtt                                                                                                                                                                  >  2:190437/1‑273 (MQ=255)                                                                                                                                                           aCTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCCGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAacaaa                                                                                                                                             >  2:1762/1‑287 (MQ=255)                                                                                                                                                                aTGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTAGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTACGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCa                                                                                                                               >  2:204818/1‑298 (MQ=255)                                                                                                                                                                  gggAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGat                                                                                                                           <  2:331215/300‑1 (MQ=255)                                                                                                                                                                       aCGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGtt                                                                                                                      <  2:535108/300‑1 (MQ=255)                                                                                                                                                                             gACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAg                                                                                                                 <  1:564575/299‑1 (MQ=255)                                                                                                                                                                              aCTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGAAGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAAAAGaa                                                                                                               >  2:120683/1‑300 (MQ=255)                                                                                                                                                                              aCTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTAAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGCTTGCCTCCGGTGGCGCGGGCACCATGGCACACTTCCTCGACGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAACCAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGaa                                                                                                               >  1:331015/1‑300 (MQ=255)                                                                                                                                                                                 gggTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGaacaa                                                                                                            >  1:212527/1‑300 (MQ=255)                                                                                                                                                                                  ggTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGaacaac                                                                                                           >  1:482915/1‑300 (MQ=255)                                                                                                                                                                                   gTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGaccaaca                                                                                                          >  2:332470/1‑300 (MQ=255)                                                                                                                                                                                   gTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGaacaaca                                                                                                          >  1:56085/1‑300 (MQ=255)                                                                                                                                                                                   gTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGaacaaca                                                                                                          >  2:507872/1‑300 (MQ=255)                                                                                                                                                                                   gTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCAGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAAAAGaacaaca                                                                                                          >  2:488813/1‑300 (MQ=255)                                                                                                                                                                                                           gCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAAATGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGTAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGACTTAAAAGCGTACCTGGCCCCACAGGGCGTGGAGATCAGGATATGTTAAAAGAACAACAACGTAGCGAACTGGACTggggcaa                                                                                  >  2:3456/1‑296 (MQ=255)                                                                                                                                                                                                           gCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCAAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGATGGCGCGGGCACCATGGAACACTTCCCCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACACATAATCAATATTGGTGAATTAAAAGCGCACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTACCAGAACCACAACGTAGCGAactggcct                                                                                         >  2:3546/1‑290 (MQ=255)                                                                                                                                                                                                           gCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGAACTAGAACAAATGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAAAAGAACAACAACGTCGCGAACTGGACTGGGaaaa                                                                                  >  2:2017/1‑300 (MQ=255)                                                                                                                                                                                                            ccGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGaaaa                                                                                  >  2:128110/1‑299 (MQ=255)                                                                                                                                                                                                                                             aCGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAacac                                                <  2:450276/300‑1 (MQ=255)                                                                                                                                                                                                                                                         aCGGTTACGACCGCGAACAACTGAAAAAAGTGCTTGA‑GTTTGCCACGTCCCACTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGc                                    <  2:56019/300‑1 (MQ=255)                                                                                                                                                                                                                                                           ggTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGCGa                                  <  1:295069/300‑1 (MQ=255)                                                                                                                                                                                                                                                           ggTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGCGa                                  >  2:227445/1‑300 (MQ=255)                                                                                                                                                                                                                                                           ggTTACGACCTCGAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCAGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAAAAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGc                                    >  2:141118/1‑298 (MQ=255)                                                                                                                                                                                                                                                                 gACCTCGAACAAATGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGTCGACGTTTACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAGTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGCGAAGTGc                             <  2:291617/299‑1 (MQ=255)                                                                                                                                                                                                                                                                       gAACAACTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGCGAAGTGCTAATGc                       <  1:204818/299‑1 (MQ=255)                                                                                                                                                                                                                                                                            acTGAAAAAAGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGCGAAGTGCTAATGCTGGGCt                 >  1:341950/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 aaaaaGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGCGAAGTGCTAATGCTGGGCTATATg            <  2:356399/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     aGTGCGTGA‑GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGCGAAGTGCTAATGCTGGGCTATATGAAcc        <  2:480605/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            tgaGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGTGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAg  <  2:111615/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                          CTCTCCTGCGCTGGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAG  >  NC_000913/2096152‑2096734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑