Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,264,748	T→C	A145A (GCT→GCC)	dnaB →	replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,264,748	0	T	C	96.4%	289.6 / NA	84	A145A (GCT→GCC)	dnaB	replicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (44/37);  minor base A (3/0);  total (47/37)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGGGATGATGTAGCCGAGCGTGTGGTAGCAGACGAT‑TTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCCGAAGCTGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGATCTGGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCGAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGGGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAAC  >  NC_000913/4264452‑4265042                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                       cgCTGGGATGATGTAGCCGAGCGTGTGGTAGCAGACGAT‑TTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGt                                                                                                                                                                                                                                                                                                     >  1:342982/1‑300 (MQ=255)     gggATGATGTAGCCGAGCGTGTGGTAGCAGACGAT‑TTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGCATGAGA‑TTGCTGAAGCCGGtttt                                                                                                                                                                                                                                                                                                  >  2:354920/1‑299 (MQ=255)     gggATGATGTAGCCGAGCGTGTGGTAGCAGACGAT‑TTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCAGTTGTCCGTGAGATGATCTAGGTTGCGAATGAGA‑TTGCTGAAGCCGGtttt                                                                                                                                                                                                                                                                                                  >  1:96940/1‑299 (MQ=255)     gggATGATGTAGCCGAGCGTGTGGTAGCAGACGAT‑TTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCAAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGtttt                                                                                                                                                                                                                                                                                                  >  1:97630/1‑299 (MQ=255)        atgatgTAGCCGAGCGTGTGGTAGCAGACGAT‑TTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCATTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATc                                                                                                                                                                                                                                                                                              <  1:288491/300‑1 (MQ=255)                gCCGAGCGTGTGGTAGCATACGAT‑TTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAgggg                                                                                                                                                                                                                                                                                      <  2:445102/300‑1 (MQ=255)                        tgtgGTAGCAGACGATNTTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTAGGTTGGGAATGAGNGTTGCTGAAACCGGttttngcccccaagggggtccc                                                                                                                                                                                                                                                                                >  1:465854/1‑282 (MQ=255)                        tgtgGTAGCAGACGAT‑TTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGTGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGTGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGACTGAGA‑TTGCTGAAGCCGGTTTTGATCCTCAGGGGCGTCCCa                                                                                                                                                                                                                                                                               >  2:161512/1‑299 (MQ=255)                                    cGAT‑TTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATctgct                                                                                                                                                                                                                                                                  >  1:270158/1‑300 (MQ=255)                                     gAT‑TTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATctgctg                                                                                                                                                                                                                                                                 <  1:61745/300‑1 (MQ=255)                                         ttttACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCNAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGac                                                                                                                                                                                                                                                              <  2:394151/300‑2 (MQ=255)                                         ttctACACCCACCCACACCGTCATATCTTTACTGAAACGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTTGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTACGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATTAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGac                                                                                                                                                                                                                                                              <  2:102038/297‑2 (MQ=255)                                           ttACACCCGCCCACACCGTCATATCTTTACTTAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTTCGGAATCGCTGGAACGCCATGTGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGac                                                                                                                                                                                                                                                              <  2:55041/298‑2 (MQ=255)                                                  cGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCTGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGaa                                                                                                                                                                                                                                                     >  1:44233/1‑300 (MQ=255)                                                  cGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGTGGCTAACATCAGTGCATATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTAGATTGCGAATGAGA‑TTGCTGAAGCCGGTTTTAATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGaa                                                                                                                                                                                                                                                     >  2:102718/1‑300 (MQ=255)                                                  cGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGaa                                                                                                                                                                                                                                                     >  2:102930/1‑300 (MQ=255)                                                  cGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCAAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCAAAGTGCGGCTAACATCAGTGCATATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCAGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATATGCTGGACCTTGCTGaa                                                                                                                                                                                                                                                     >  2:124481/1‑300 (MQ=255)                                                   gCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTg                                                                                                                                                                                                                                                       <  1:73226/297‑1 (MQ=255)                                                   gCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAAt                                                                                                                                                                                                                                                    <  1:286247/300‑1 (MQ=255)                                                       acgccttcgtgtCTTTATTTAAATGTCGCGTTTGCAGGAGAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGTGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACAGCAGTACCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATcc                                                                                                                                                                                                                                                  <  2:113892/287‑1 (MQ=255)                                                          ccGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGt                                                                                                                                                                                                                                             >  1:391069/1‑300 (MQ=255)                                                           cGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTc                                                                                                                                                                                                                                            >  2:514529/1‑300 (MQ=255)                                                            gTCATATCTTTACTGAAATGGCGCGTTTGCAGGCAAGCGATAGCCCTATCGATCTGATTCCTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTCTCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTCTGCGGCCATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGCAGCCGATTTTGATCAGCAGGGGCGTACCAGCGAAGATCTGCTGGACCCTGCTGAATCCCGCGTCt                                                                                                                                                                                                                                           >  1:512360/1‑300 (MQ=255)                                                            gTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCt                                                                                                                                                                                                                                           >  1:305816/1‑300 (MQ=255)                                                            gTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCt                                                                                                                                                                                                                                           >  2:515017/1‑300 (MQ=255)                                                            gTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGAACTTGCTGAATCCCGCGTCt                                                                                                                                                                                                                                           >  2:246025/1‑300 (MQ=255)                                                            gTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGCAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAACGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCAGCGTCt                                                                                                                                                                                                                                           >  2:515027/1‑300 (MQ=255)                                                            gTCATATCTTTAATGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCt                                                                                                                                                                                                                                           >  1:562616/1‑300 (MQ=255)                                                                                            gAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGAc                                                                                                                                                                                                           <  1:389665/300‑1 (MQ=255)                                                                                                 cGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGaa                                                                                                                                                                                                        >  1:133887/1‑298 (MQ=255)                                                                                                      gCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCga                                                                                                                                                                                                 >  1:18677/1‑300 (MQ=255)                                                                                                         cTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCgaaga                                                                                                                                                                                              >  1:190862/1‑300 (MQ=255)                                                                                                          tATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGTCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCGGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCgaag                                                                                                                                                                                               <  1:163245/298‑1 (MQ=255)                                                                                                              gATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATc                                                                                                                                                                                         <  1:357696/300‑1 (MQ=255)                                                                                                                             gCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGAc                                                                                                                                                                          <  1:490720/300‑1 (MQ=255)                                                                                                                               ggagtCGCTGGAACTTCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAATGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGTTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTGTAAAATGGCCGAAAGTCGTGCAAACAAAGACGAAGGGTCGAAGAACATCGCCGATGTGCTCGACGc                                                                                                                                                                        <  2:121136/296‑1 (MQ=255)                                                                                                                                taaTCGCTGGACCGTCATGTGCAACTCGATATCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACTCCAAGTGCTGCTAACATCAGTGCCTATGCGGACATCATGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑GTGNTGAAGCCGGTTTTGATCCGCAGGGGCGTACCANCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGc                                                                                                                                                                        <  2:411587/298‑1 (MQ=255)                                                                                                                                 aaTCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGNTGAAGCCGGTTTTGATCCGCAGGGGCGTACCANCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGc                                                                                                                                                                        <  2:411583/298‑1 (MQ=255)                                                                                                                                     gCTGGAACGCCATGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGGCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTTAGATGATCTCGGTTGCGAATGAGA‑TTGCTTAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGGGCAAACAAAGACGAAGGGCCTAAGAACATCGCCGATGTGCTCGACGCAACCg                                                                                                                                                                   <  2:75560/299‑1 (MQ=255)                                                                                                                                      cTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGt                                                                                                                                                                  >  1:451200/1‑299 (MQ=255)                                                                                                                                         gAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGgcg                                                                                                                                                              <  1:138756/300‑1 (MQ=255)                                                                                                                                            cGCCAGGGGCAACTCGNTAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTGAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAANAACATCGCCGATGTGCTCNACGCAACCGTGGCGCGt                                                                                                                                                           >  1:473744/1‑300 (MQ=255)                                                                                                                                            cGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCAGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGACGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGt                                                                                                                                                           >  2:479610/1‑300 (MQ=255)                                                                                                                                                  gggCAACTCGATAGTGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCAGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGa                                                                                                                                                      >  1:173025/1‑299 (MQ=255)                                                                                                                                                  gggCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGa                                                                                                                                                      >  1:202140/1‑299 (MQ=255)                                                                                                                                                   gtcAACTCGATAGCGTTGGGGGTTTTGCTTATCTGGCAGAGTTGTCAAAAAATACGCCAAGTGCGGTTAACATCAGTGCCTATGCGGACATCGTGCTTGAACGTGCCGTTGTCCGTTAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGc                                                                                                                                                    <  1:78243/298‑1 (MQ=255)                                                                                                                                                   ggCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGc                                                                                                                                                    <  1:78252/300‑1 (MQ=255)                                                                                                                                                     cAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCCAACGTGGTGCGTATTGAGCa                                                                                                                                                   >  2:22553/1‑299 (MQ=255)                                                                                                                                                              atcgtcgtTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTcag                                                                                                                                         <  1:23019/298‑1 (MQ=255)                                                                                                                                                              aGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTcag                                                                                                                                         <  2:558005/300‑1 (MQ=255)                                                                                                                                                                  tCGGTGTTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCGAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTGGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCAGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCagcagc                                                                                                                                     <  1:49966/300‑1 (MQ=255)                                                                                                                                                                        gtTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACg                                                                                                                               <  1:446827/300‑1 (MQ=255)                                                                                                                                                                               ttATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGt                                                                                                                        <  1:304871/300‑1 (MQ=255)                                                                                                                                                                                 aTCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTAGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTa                                                                                                                      >  1:295775/1‑300 (MQ=255)                                                                                                                                                                                 aTCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTAGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCCTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTa                                                                                                                      >  2:266044/1‑300 (MQ=255)                                                                                                                                                                                    tGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTAcc                                                                                                                    <  2:259487/299‑1 (MQ=255)                                                                                                                                                                                     ggCAGAGCTGTCAAAAAATACGCCAAGTGCTGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCgg                                                                                                                  <  2:326641/300‑1 (MQ=255)                                                                                                                                                                                                   aaaaTACTCCAGGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGATGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGtt                                                                                                    <  2:554197/300‑1 (MQ=255)                                                                                                                                                                                                    aaaTACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTa                                                                                                   >  2:130596/1‑300 (MQ=255)                                                                                                                                                                                                                    tcTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACaaa                                                                                   >  1:496634/2‑300 (MQ=255)                                                                                                                                                                                                                    gCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAGGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACaaa                                                                                   >  1:206132/1‑300 (MQ=255)                                                                                                                                                                                                                    gCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACaaa                                                                                   >  1:206126/1‑300 (MQ=255)                                                                                                                                                                                                                    gCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTAAACaaa                                                                                   >  2:336038/1‑300 (MQ=255)                                                                                                                                                                                                                          attagtaccTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCTTGAGATGGTCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGAc                                                                                               <  2:118129/275‑1 (MQ=255)                                                                                                                                                                                                                          aTCAGTGCCTATGTGGACATCATGCGTGAACGTGCCGTTGTCCGTTAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCATGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCTAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAAc                                                                              <  2:14301/299‑1 (MQ=255)                                                                                                                                                                                                                                gCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCAGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGc                                                                       >  2:507422/1‑300 (MQ=255)                                                                                                                                                                                                                                   tATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCtt                                                                     >  1:200051/1‑299 (MQ=255)                                                                                                                                                                                                                                      gcgggtaTCGTGCGTGGACGTGTTGTTGTGCGTGAGGTGATTTTGGTTGCGGATGAGA‑TTGTTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGTTGAATCCCGCGTCTTTAAAATTGCCGAAGGTCGTGCAAACAAAGACGAAGGGTCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAGCCGTTGGCTTGCAg                                                                 <  2:55965/294‑1 (MQ=255)                                                                                                                                                                                                                                        ggACATCGTGCGTGAACGTGCCGTTGTCCGTTAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGAGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGc                                                                <  1:432980/299‑1 (MQ=255)                                                                                                                                                                                                                                         gACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGcc                                                               >  1:320366/1‑299 (MQ=255)                                                                                                                                                                                                                                         gACATCGTGCGGGAACGTGCCGTTGTCCGGGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCg                                                              <  2:342982/300‑1 (MQ=255)                                                                                                                                                                                                                                           cATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTc                                                            >  1:356290/1‑300 (MQ=255)                                                                                                                                                                                                                                               gtgcgtgAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCANCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGAt                                                        >  1:397905/1‑300 (MQ=255)                                                                                                                                                                                                                                                tgcgtgAACGTGCTGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGa                                                         <  2:386794/298‑1 (MQ=255)                                                                                                                                                                                                                                                tgcgtgAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACCCCGGTTATGACGATCTCCACAAAAAAACCGCTGGCCTGCAGCCGTCGGAtt                                                       >  2:514081/1‑300 (MQ=255)                                                                                                                                                                                                                                                     gAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGatc                                                  <  1:42088/300‑1 (MQ=255)                                                                                                                                                                                                                                                      aaCGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGatc                                                  >  1:56225/1‑299 (MQ=255)                                                                                                                                                                                                                                                         gTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGatcatc                                               >  1:314810/1‑299 (MQ=255)                                                                                                                                                                                                                                                         gTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCAGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTAAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAACCATCGGAtt                                                       >  2:48971/1‑291 (MQ=255)                                                                                                                                                                                                                                                               ttGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTcgccg                                        <  1:262890/300‑1 (MQ=255)                                                                                                                                                                                                                                                               ttGTCCGTGAGATGATCTCGGTTGCAAATGAGA‑TTGCTGAAGCAGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTNGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTAAACAAAAAAACCGCTGGCTTGCAGCCGTAGGATTTGATCATCGTcgcc                                         >  2:165660/1‑299 (MQ=255)                                                                                                                                                                                                                                                                 gTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACNGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTcgccgc                                       >  1:108239/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                      gCGAATGAGA‑TTGCTGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAaaca                 >  2:317659/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                      gAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTTGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGAGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAAc  <  1:144657/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                       CGCTGGGATGATGTAGCCGAGCGTGTGGTAGCAGACGAT‑TTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTGCAGGAAAGCGGTAGCCCTATCGATCTGATTACTCTTGCGGAATCGCTGGAACGCCAGGGGCAACTCGATAGCGTCGGTGGTTTTGCTTATCTGGCAGAGCTGTCAAAAAATACGCCAAGTGCGGCTAACATCAGTGCCTATGCGGACATCGTGCGTGAACGTGCCGTTGTCCGTGAGATGATCTCGGTTGCGAATGAGA‑TTGCCGAAGCTGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGATCTGGCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCGAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGACGCAACCGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGGGTAAACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAAC  >  NC_000913/4264452‑4265042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑