Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,424,009	C→A	Q104K (CAG→AAG)	ulaF →	L‑ribulose‑5‑phosphate 4‑epimerase UlaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,424,009	0	C	A	100.0%	204.2 / NA	58	Q104K (CAG→AAG)	ulaF	L‑ribulose‑5‑phosphate 4‑epimerase UlaF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/30);  total (28/30)

CAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGC  >  NC_000913/4423718‑4424290                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                          cagGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGc                                                                                                                                                                                                                                                                                       <  1:337730/296‑1 (MQ=255)       tttGAAGCCAACATGGAGCTGCCGCGCTACGTGCTGGTGACCTTTACCTGGGGCAACGTCGACGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCg                                                                                                                                                                                                                                                                             <  1:269831/300‑1 (MQ=255)       tttGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCg                                                                                                                                                                                                                                                                             <  1:229294/300‑1 (MQ=255)                 aCATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTAGAACTCTAACGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGAGATccc                                                                                                                                                                                                                                                                        >  2:269636/1‑295 (MQ=255)                      gAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGc                                                                                                                                                                                                                                                              >  1:342086/1‑300 (MQ=255)                                gcTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGACGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTAGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACcacgcac                                                                                                                                                                                                                                                     >  2:210205/1‑299 (MQ=255)                                          ggTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCAAGCACGCCGACTActt                                                                                                                                                                                                                                          >  1:130923/1‑300 (MQ=255)                                                          ggCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTAGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCTAACTACTTATTTGGCGACATTcc                                                                                                                                                                                                                           >  2:36468/1‑299 (MQ=255)                                                          ggCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAAACATGAAAGCAGCCGATATGGTGGTGATTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTAGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACAt                                                                                                                                                                                                                              >  2:36310/1‑296 (MQ=255)                                                                   aGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTAcgcg                                                                                                                                                                                                                  <  1:516769/299‑1 (MQ=255)                                                                             aCCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCGTCCGACACTGCGACACATCTCGAACTCTACCGTCGTTACCCTTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTGAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCg                                                                                                                                                                                                       <  2:521792/300‑1 (MQ=255)                                                                                         ggCTGTTGGTGATCAGGCCCAGCGTCGTTGCCGACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGTTGGTGGAATGGGAGTATCGCCCATCTTCCGACACTGCGACGCACCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCTGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAATAAGAGGTGc                                                                                                                                                                                           <  2:120433/300‑1 (MQ=255)                                                                                         ggCTGGTGGGGATCAAGCCCAGCGGTGTTGGCTACGAAATCATGAAAGCGGCTGATATGGTGGTGGTTGATATGAGCGGCAGTGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATTTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGTGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGTTTAAGCGAATAAGAGGTGc                                                                                                                                                                                           <  2:185126/300‑1 (MQ=255)                                                                                             ggtggtGATAAAGCCCAGCGGCGGTGCCTAAGAAACCATGAAAGCGGCCGATATGGGGGTGGGTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTTCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTTGCATTGGCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCTGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAgtg                                                                                                                                                                                       <  2:377637/300‑3 (MQ=255)                                                                                              gtggtgATCAAGCCCAGCGGCGTTGCCTACTAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGc                                                                                                                                                                                      <  1:141418/300‑1 (MQ=255)                                                                                                    aTCAAGCCCAGTGGCGTGGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGTAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAt                                                                                                                                                                                <  1:190086/300‑1 (MQ=255)                                                                                                    aTCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAt                                                                                                                                                                                >  1:316172/1‑300 (MQ=255)                                                                                                     tCAAGCCCAGCGGCGTTGCCTACTAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGTGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGc                                                                                                                                                                                      <  2:140509/293‑1 (MQ=255)                                                                                                        agcccattgtcgTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGTTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGTTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGACCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaac                                                                                                                                                                            <  2:106722/290‑1 (MQ=255)                                                                                                        aGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCNATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaac                                                                                                                                                                            <  2:440863/300‑1 (MQ=255)                                                                                                        aGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaac                                                                                                                                                                            <  1:486512/300‑1 (MQ=255)                                                                                                             aGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTAtgaactgaac                                                                                                                                                                       <  1:358905/300‑1 (MQ=255)                                                                                                                      gCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGACGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCaaa                                                                                                                                                              >  1:438214/1‑300 (MQ=255)                                                                                                                        cTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGt                                                                                                                                                            >  2:392054/1‑300 (MQ=255)                                                                                                                         tatgAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGc                                                                                                                                                                                           <  2:184882/265‑1 (MQ=255)                                                                                                                                ccATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATANCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATc                                                                                                                                                     >  2:402565/1‑299 (MQ=255)                                                                                                                                     aaaGCGGCCGATATGGNGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGANTATGAACTGAACACCGGCNAAGTGATTATCGAAAc                                                                                                                                                >  1:471889/1‑299 (MQ=255)                                                                                                                                               atatGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGNGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAAc                                                                                                                                      >  1:395190/1‑299 (MQ=255)                                                                                                                                                tatGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGc                                                                                                                                    <  1:228204/300‑1 (MQ=255)                                                                                                                                                     tggtggtTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGc                                                                                                                               <  2:474557/300‑1 (MQ=255)                                                                                                                                                      gttggtTGATATGATCGGTAAGGTGGTGGAAGGGGAGTGTCGCCCATCTTCCGGCATTGCGACGCATCTCGAACTCTACCGTCGTTACCCTTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGTGCTGGCAACCCCTGCGTTAGGCACCACGCACGCCGACTACTTCTTTTGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAATTGATTATCGAAACGCTGGGCAACGc                                                                                                                                    <  2:30880/292‑1 (MQ=255)                                                                                                                                                        tggtTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGc                                                                                                                            >  1:453137/1‑300 (MQ=255)                                                                                                                                                          gtTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCt                                                                                                                           >  1:344971/1‑299 (MQ=255)                                                                                                                                                                  gAGCGGCAAGGTGGTGGAAGGGGGGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTGCCGTCGTTACCCGTCGCTTGGTTGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTTGCGACATTCCGTGTACGCGCGGGGTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCAtgcgtc                                                                                                                  <  2:90081/300‑6 (MQ=255)                                                                                                                                                                           ggtggtGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCGTGTTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGNCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCTAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATtgt                                                                                                         <  2:181497/300‑1 (MQ=255)                                                                                                                                                                              ggtggAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACNACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTgtgg                                                                                                       <  2:411075/299‑1 (MQ=255)                                                                                                                                                                                       ggAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCa                                                                                             <  1:382427/300‑1 (MQ=255)                                                                                                                                                                                             tCGCCCATCTTCCGACACTTCGACGCATCTCAAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGTGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGcc                                                                                       <  2:242110/300‑1 (MQ=255)                                                                                                                                                                                             tCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTTGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCTAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGcc                                                                                       <  2:242116/300‑1 (MQ=255)                                                                                                                                                                                                   aTCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGc                                                                                 >  1:510118/1‑300 (MQ=255)                                                                                                                                                                                                     cTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGcat                                                                               >  2:338063/1‑298 (MQ=255)                                                                                                                                                                                                      ttCCGACACTGCGACGCATCTAGAACTCTAACGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGANCACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCt                                                                               >  2:439364/1‑299 (MQ=255)                                                                                                                                                                                                                               ctctACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAATGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGt                                                      >  2:55385/1‑299 (MQ=255)                                                                                                                                                                                                                                      gtcgtTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATTGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGTCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAATAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGTAAAGATGCTCACGATGCGGTGCATAAc                                               <  1:210866/299‑1 (MQ=255)                                                                                                                                                                                                                                      gtcgtTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTTGGGGAAAGATGCTCACGATGCGGTGCATAAc                                               <  1:210830/299‑1 (MQ=255)                                                                                                                                                                                                                                      gtcgtTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAAc                                               <  1:211088/299‑1 (MQ=255)                                                                                                                                                                                                                                       tcgtTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAAcgc                                             <  2:342086/300‑1 (MQ=255)                                                                                                                                                                                                                                            aCCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCggtgg                                        <  1:557496/300‑1 (MQ=255)                                                                                                                                                                                                                                                                gTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaa                    >  2:261948/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaa                    >  1:451577/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAAAGAGGTGGTGATGGAAGAAGTGGCGaaa                    >  2:521054/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gTCCATACCCACTCCACTCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCAGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAAAGCGGTGGTGATGGAAGAAGTGGCGaaa                    >  2:144395/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gTCCATACCCACTCCACTCATGCCACCGCATGGGCAAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCAAGCACGCCGACTACTTATTTGGCGACATTCAGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGAACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaa                    >  2:243169/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gTCCATACCCACTCCAATCATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAAAGCCGAGCCGCTGCATACGCAGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTTACGATGCGGTGCATAAAGCGGTGGTGATGGAAGAAGTGGCGaaa                    >  2:202892/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          actccactcATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACNCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGa          >  2:175605/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          actccactcATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGa          >  1:220667/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               actcATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGcc     >  2:358662/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  cATGCCACCGCATGGGCGAAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCcgc  >  1:433485/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                          CAGGTATTTGAAGCCAACATGGAGCTGCCGCGCTACGGGCTGGTGACCTTTACCTGGGGCAACGTCAGCGCTATCGACCGCGAACGCGGGCTGGTGGTGATCAAGCCCAGCGGCGTTGCCTACGAAACCATGAAAGCGGCCGATATGGTGGTGGTTGATATGAGCGGCAAGGTGGTGGAAGGGGAGTATCGCCCATCTTCCGACACTGCGACGCATCTCGAACTCTACCGTCGTTACCCGTCGCTTGGTGGCATTGTCCATACCCACTCCACTCATGCCACCGCATGGGCGCAGGCGGGGCTGGCGATCCCGGCGTTAGGCACCACGCACGCCGACTACTTCTTTGGCGACATTCCGTGTACGCGCGGGTTAAGCGAAGAAGAGGTGCAGGGCGAGTATGAACTGAACACCGGCAAAGTGATTATCGAAACGCTGGGCAACGCCGAGCCGCTGCATACGCCGGGAATTGTGGTGTATCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGC  >  NC_000913/4423718‑4424290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑