breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsME8324_S5_R1_RMDUP_RM-R2-DUP.cutadapt564,687167,865,861100.0%297.3 bases300 bases95.8%
errorsME8324_S5_R2_RMDUP_RM-R1-DUP.cutadapt564,830166,627,291100.0%295.0 bases300 bases98.4%
total1,129,517334,493,152100.0%296.1 bases300 bases97.1%

Reference Sequence Information

seq idlengthfit meanfit relative_variance% mapped readsdescription
coveragedistributionNC_0009134,641,65266.26.7100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit relative_variance is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000016300
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000155
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.020

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.92081

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.8
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.5.1
R4.0.3

Execution Times

stepstartendelapsed
Read and reference sequence file input12:55:51 10 Oct 202512:56:09 10 Oct 202518 seconds
Read alignment to reference genome12:56:09 10 Oct 202512:57:08 10 Oct 202559 seconds
Preprocessing alignments for candidate junction identification12:57:08 10 Oct 202512:57:26 10 Oct 202518 seconds
Preliminary analysis of coverage distribution12:57:26 10 Oct 202512:58:12 10 Oct 202546 seconds
Identifying junction candidates12:58:12 10 Oct 202512:58:17 10 Oct 20255 seconds
Re-alignment to junction candidates12:58:17 10 Oct 202512:58:28 10 Oct 202511 seconds
Resolving best read alignments12:58:28 10 Oct 202512:59:01 10 Oct 202533 seconds
Creating BAM files12:59:01 10 Oct 202512:59:40 10 Oct 202539 seconds
Tabulating error counts12:59:40 10 Oct 202513:00:10 10 Oct 202530 seconds
Re-calibrating base error rates13:00:10 10 Oct 202513:00:11 10 Oct 20251 second
Examining read alignment evidence13:00:11 10 Oct 202513:04:22 10 Oct 20254 minutes 11 seconds
Polymorphism statistics13:04:22 10 Oct 202513:04:23 10 Oct 20251 second
Output13:04:23 10 Oct 202513:04:31 10 Oct 20258 seconds
Output :: Mutation Prediction13:04:23 10 Oct 202513:04:23 10 Oct 20250 seconds
Output :: Mutation Annotation13:04:23 10 Oct 202513:04:23 10 Oct 20250 seconds
Total 8 minutes 40 seconds