breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 17,917 G→A L143L (CTG→CTA nhaA → Na(+):H(+) antiporter NhaA
RA 35,649 G→A R172C (CGT→TGT)  caiD ← crotonobetainyl‑CoA hydratase
RA 56,141 C→T Q323Q (CAG→CAA lptD ← lipopolysaccharide assembly protein LptD
RA 69,387 C→T S221N (AGT→AAT)  araB ← ribulokinase
RA 109,960 G→A R561H (CGT→CAT)  secA → protein translocation ATPase
RA 124,134 C→T A373V (GCA→GTA)  aceE → pyruvate dehydrogenase E1 component
RA 128,449 G→A V180I (GTA→ATA)  lpd → lipoamide dehydrogenase
RA 129,640 C→T V541M (GTG→ATG)  yacH ← DUF3300 domain‑containing protein YacH
RA 137,860 G→A D260N (GAC→AAC)  cueO → multicopper oxidase CueO
RA 140,773 G→A S151S (AGC→AGT gcd ← quinoprotein glucose dehydrogenase
RA 143,354 C→T T192T (ACC→ACT yadG → putative ABC transporter ATP‑binding protein YadG
RA 143,500 C→T P241L (CCG→CTG)  yadG → putative ABC transporter ATP‑binding protein YadG
RA 151,929 C→T K101K (AAG→AAA yadL ← putative fimbrial protein YadL
RA 157,894 C→T E411E (GAG→GAA pcnB ← poly(A) polymerase I
RA 159,678 C→T Q145Q (CAG→CAA gluQ ← glutamyl‑Q tRNA(Asp) synthetase
RA 163,611 C→T R503C (CGC→TGC)  hrpB → RNA‑dependent NTPase HrpB
RA 164,911 G→A G61D (GGC→GAC) 
G61D (GGC→GAC) 
mrcB →
mrcB →
peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
RA 167,400 C→T intergenic (+136/‑84) mrcB → / → fhuA peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB/ferrichrome outer membrane transporter/phage receptor
RA 176,263 C→T A386V (GCG→GTG)  clcA → chloride:H(+) antiporter ClcA
RA 183,927 C→T V57I (GTC→ATC)  yaeH ← DUF3461 domain‑containing protein YaeH
RA 184,537 C→T S178N (AGC→AAC)  yaeI ← phosphodiesterase YaeI
RA 197,617 C→T L358F (CTC→TTC)  rseP → intramembrane zinc metalloprotease RseP
RA 200,671 C→T L64L (CTG→TTG)  skp → periplasmic chaperone Skp
RA 201,383 C→T A138V (GCC→GTC)  lpxD → UDP‑3‑O‑(3‑hydroxymyristoyl)glucosamine N‑acyltransferase
RA 202,266 C→T P56S (CCT→TCT)  fabZ → 3‑hydroxy‑acyl‑[acyl‑carrier‑protein] dehydratase
RA 205,045 C→T R185C (CGC→TGC)  rnhB → RNase HII
RA 209,910 C→T P78S (CCG→TCG)  ldcC → lysine decarboxylase 2
RA 222,052 G→A T198T (ACC→ACT metN ← L‑methionine/D‑methionine ABC transporter ATP binding subunit
RA 236,776 G→A G237E (GGA→GAA)  dnaQ → DNA polymerase III subunit epsilon
RA 239,159 G→A intergenic (‑423/+260) ykfM ← / ← yafV uncharacterized protein YkfM/2‑oxoglutaramate amidase
RA 241,309 G→A N665N (AAC→AAT fadE ← acyl‑CoA dehydrogenase
RA 241,648 G→A R552R (CGC→CGT fadE ← acyl‑CoA dehydrogenase
RA 242,499 G→A L269L (CTG→TTG)  fadE ← acyl‑CoA dehydrogenase
RA 242,847 G→A P153S (CCG→TCG)  fadE ← acyl‑CoA dehydrogenase
RA 246,272 G→A A77A (GCC→GCT dinJ ← antitoxin/DNA‑binding transcriptional repressor DinJ
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
RA 247,154 G→A S148N (AGC→AAC)  yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
JC JC 331,730 IS2 (–) +5 bp intergenic (+234/‑68) betT → / → yahV choline:H(+) symporter/protein YahV
RA 332,716 G→A A116T (GCG→ACG)  pdeL → DNA‑binding transcriptional regulator/c‑di‑GMP phosphodiesterase PdeL
RA 339,935 C→T A56V (GCC→GTC)  yahH → putative uncharacterized protein YahH
RA 339,941 C→T T58I (ACT→ATT)  yahH → putative uncharacterized protein YahH
RA 339,958 G→A D64N (GAC→AAC)  yahH → putative uncharacterized protein YahH
RA 374,972 T→C intergenic (+91/‑487) mhpE → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 374,974 A→G intergenic (+93/‑485) mhpE → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 374,986 T→C intergenic (+105/‑473) mhpE → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 388,732 C→T intergenic (+86/+21) tauD → / ← hemB taurine dioxygenase/porphobilinogen synthase
RA 408,799 C→T intergenic (+338/‑148) ppnP → / → ykiD nucleoside phosphorylase PpnP/protein YkiD
RA 475,920 C→T S181F (TCT→TTT)  ybaY → PF09619 family lipoprotein YbaY
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
RA 480,398 C→T G104D (GGT→GAT)  tomB ← Hha toxicity modulator TomB
RA 526,759 G→A A1167T (GCG→ACG)  rhsD → protein RhsD
RA 567,809 C→T C224C (TGC→TGT insF3 → IS3 element protein InsF
RA 590,565 G→A S130S (AGC→AGT nfrA ← exopolysaccharide secretion system outer membrane protein NfrA
RA 596,410 G→A G271R (GGA→AGA)  cusC → copper/silver export system outer membrane channel
RA 647,673 C→T W121* (TGG→TGA citX ← apo‑citrate lyase phosphoribosyl‑dephospho‑CoA transferase
MC JC 683,267 Δ4,584 bp IS5‑mediated [hscC]gltI [hscC], rihA, gltL, gltK, gltJ, sroC, gltI
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
RA 722,884 G→A R511C (CGC→TGC)  kdpD ← sensor histidine kinase KdpD
RA 734,575 G→A intergenic (+473/‑1075) ybfB → / → ybfC uncharacterized protein YbfB/uncharacterized protein YbfC
RA 734,857 C→T intergenic (+755/‑793) ybfB → / → ybfC uncharacterized protein YbfB/uncharacterized protein YbfC
RA 784,488 G→A R112R (CGC→CGT zitB ← Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter
RA 804,359 G→A G286D (GGC→GAC)  ybhJ → putative hydratase YbhJ
RA 807,730 G→A G60G (GGC→GGT ybhB ← putative kinase inhibitor
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 839,446 C→T G696R (GGA→AGA)  fiu ← iron catecholate outer membrane transporter Fiu
RA 841,518 C→T R5H (CGC→CAC)  fiu ← iron catecholate outer membrane transporter Fiu
RA 844,731 C→T E250E (GAG→GAA ybiO ← moderate conductance mechanosensitive channel YbiO
RA 850,443 G→A intergenic (+46/‑7) yliM → / → ompX protein YliM/outer membrane protein X
RA 850,931 G→A G161D (GGC→GAC)  ompX → outer membrane protein X
RA 866,651 C→T Y28Y (TAC→TAT iaaA → isoaspartyl dipeptidase proenzyme
RA 899,762 C→T intergenic (+117/+82) rlmC → / ← artJ 23S rRNA m(5)U747 methyltransferase/L‑arginine ABC transporter periplasmic binding protein
RA 922,086 C→T C580C (TGC→TGT macB → ABC‑type tripartite efflux pump ATP binding/membrane subunit
RA 923,400 C→T A46V (GCG→GTG)  clpA → ATP‑dependent Clp protease ATP‑binding subunit ClpA
RA 946,122 C→T G84G (GGC→GGT ycaD → putative transporter YcaD
RA 961,394 C→T S65F (TCC→TTC)  cmk → cytidylate kinase
RA 963,617 C→T N541N (AAC→AAT rpsA → 30S ribosomal subunit protein S1
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 974,351 G→A L11L (CTG→CTA mukF → chromosome partitioning protein MukF
RA 1,003,511 G→A G25S (GGC→AGC)  ycbV → putative fimbrial protein YcbV
RA 1,007,235 C→T W122* (TGG→TGA ycbX ← 6‑N‑hydroxylaminopurine resistance protein
RA 1,038,613 C→T L292L (CTA→TTA)  appC → cytochrome bd‑II subunit 1
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
RA 1,042,957 C→T Q418Q (CAG→CAA etk ← protein‑tyrosine kinase Etk
RA 1,049,328 C→T R2H (CGC→CAC)  gfcB ← lipoprotein GfcB
RA 1,060,831 C→T P526S (CCT→TCT)  torA → trimethylamine N‑oxide reductase 1
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,077,020 C→T G621G (GGG→GGA putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,080,930 C→T intergenic (+117/‑1313) putP → / → efeO proline:Na(+) symporter/ferrous iron transport system protein EfeO
RA 1,094,037 C→T R388C (CGT→TGT)  dgcT → putative diguanylate cyclase DgcT
RA 1,104,125 C→T Q59* (CAG→TAG)  csgB → curlin, minor subunit
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,118,119 C→T D120N (GAT→AAT)  yceI ← protein YceI
RA 1,122,318 C→T G97D (GGC→GAC)  pyrC ← dihydroorotase
RA 1,128,789 G→A L317L (TTG→TTA murJ → lipid II flippase MurJ
RA 1,130,025 G→A A35V (GCC→GTC)  flgM ← anti‑sigma factor for FliA (sigma(28))
RA 1,134,466 C→T A222V (GCC→GTC)  flgF → flagellar basal‑body rod protein FlgF
RA 1,153,622 C→T A108V (GCT→GTT)  pabC → aminodeoxychorismate lyase
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,181,346 C→T G127D (GGC→GAC)  ymfA ← putative inner membrane protein YmfA
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
JC JC 1,286,488 IS1 (–) +9 bp coding (640‑648/678 nt) narI → nitrate reductase A subunit gamma
RA 1,293,531 C→T A2V (GCA→GTA)  tdk → thymidine/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,301,788 G→T E203* (GAA→TAA)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,313,422 G→A A81V (GCT→GTT)  yciC ← putative inner membrane protein
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,340,778 C→T T74M (ACG→ATG)  lapB → lipopolysaccharide assembly protein B
RA 1,342,801 C→T C48C (TGC→TGT yciH → putative translation factor
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,361,174 C→T L19F (CTT→TTT)  puuD → gamma‑glutamyl‑gamma‑aminobutyrate hydrolase
RA 1,364,324 C→T C31C (TGC→TGT puuB → gamma‑glutamylputrescine oxidase
RA 1,395,234 C→T G230E (GGA→GAA)  ynaI ← small conductance mechanosensitive channel YnaI
RA 1,408,877 C→T V276V (GTC→GTT zntB → Zn(2(+)):H(+) symporter
RA 1,437,952 G→A L945L (CTG→TTG)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,438,813 C→T A658T (GCG→ACG)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,439,116 G→A R557C (CGT→TGT)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,441,990 G→A S285F (TCT→TTT)  ldhA ← D‑lactate dehydrogenase
RA 1,443,674 G→A G208G (GGG→GGA ydbH → putative phospholipid transport factor YdbH
RA 1,446,567 C→T L239L (TTG→TTA feaR ← DNA‑binding transcriptional activator FeaR
RA 1,511,403 G→A R467R (CGG→CGA ydcR → fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR
RA 1,513,915 C→T L29L (CTG→TTG)  ydcU → putative ABC transporter membrane subunit YdcU
RA 1,513,927 C→T L33L (CTG→TTG)  ydcU → putative ABC transporter membrane subunit YdcU
RA 1,567,494 G→A intergenic (‑275/+10) yddY ← / ← digH protein YddY/divisome localized glycosyl hydrolase
RA 1,568,454 G→A P124S (CCG→TCG)  digH ← divisome localized glycosyl hydrolase
RA 1,570,361 G→A V43V (GTC→GTT gadC ← L‑glutamate:4‑aminobutyrate antiporter
RA 1,581,258 G→A P423S (CCC→TCC)  ydeN ← putative sulfatase YdeN
RA 1,582,247 G→A T93I (ACC→ATC)  ydeN ← putative sulfatase YdeN
RA 1,588,678 G→A N54N (AAC→AAT ydeS ← putative fimbrial protein YdeS
RA 1,590,339 G→A intergenic (‑338/+515) ydeT ← / ← hipA fimbrial usher domain‑containing protein YdeT/serine/threonine‑protein kinase toxin HipA
RA 1,615,674 G→A G291R (GGA→AGA)  yneJ → putative DNA‑binding transcriptional regulator YneJ
RA 1,615,856 G→A D32N (GAT→AAT)  yneK → protein YneK
RA 1,618,833 C→T A26T (GCG→ACG)  marC ← inner membrane protein MarC
RA 1,623,146 G→A N235N (AAC→AAT dgcZ ← diguanylate cyclase DgcZ
RA 1,623,788 G→A A21A (GCC→GCT dgcZ ← diguanylate cyclase DgcZ
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,655,010 G→A A44A (GCC→GCT rspA ← mandelate racemase/muconate lactonizing enzyme family protein RspA
RA 1,711,083 C→T L86L (CTG→TTG)  rsxE → SoxR [2Fe‑2S] reducing system protein RsxE
RA 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI
RA 1,728,390 G→A R15H (CGT→CAT)  rnt → ribonuclease T
RA 1,735,948 C→T L191F (CTC→TTC)  sodB → superoxide dismutase (Fe)
RA 1,741,364 C→T intergenic (+242/‑49) punC → / → cfa purine nucleoside transporter PunC/cyclopropane fatty acyl phospholipid synthase
RA 1,744,269 C→T V271V (GTC→GTT mdtK → multidrug efflux pump MdtK
RA 1,747,368 C→T H80Y (CAT→TAT)  ydhS → FAD/NAD(P) binding domain‑containing protein YdhS
RA 1,755,663 C→T F56F (TTC→TTT ynhH → protein YnhH
RA 1,755,879 C→T A61V (GCC→GTC)  pykF → pyruvate kinase 1
RA 1,757,363 C→T intergenic (+253/‑58) pykF → / → lpp pyruvate kinase 1/murein lipoprotein
RA 1,776,715 A→T S377C (AGT→TGT)  ydiF → putative acetate‑CoA transferase YdiF
JC JC 1,785,887 IS30 (–) +2 bp coding (1225‑1226/2379 nt) ppsA ← phosphoenolpyruvate synthetase
RA 1,819,438 G→A P4S (CCA→TCA)  chbR ← ChbR
RA 1,867,987 G→A T360T (ACG→ACA yeaG → protein kinase YeaG
JC JC 1,879,829 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,886,565 C→T R241C (CGT→TGT)  yeaX → carnitine monooxygenase subunit YeaX
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,899,926 G→A Q500Q (CAG→CAA pdeD → putative c‑di‑GMP phosphodiesterase PdeD
RA 1,913,994 C→T A275T (GCT→ACT)  prc ← tail‑specific protease
RA 1,987,499 C→A intergenic (+71/+8) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
RA 1,998,888 G→A T198I (ACT→ATT)  dcyD ← D‑cysteine desulfhydrase
RA 2,022,111 C→T P81P (CCC→CCT fliP → flagellar biosynthesis protein FliP
RA 2,023,126 C→T I78I (ATC→ATT fliR → flagellar biosynthesis protein FliR
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,058,789 C→T G353E (GGG→GAG)  yeeO ← FMN/FAD exporter
RA 2,082,218 C→T L110L (TTG→TTA dacD ← D‑alanyl‑D‑alanine carboxypeptidase DacD
RA 2,122,019 C→T E321K (GAA→AAA)  cpsG ← phosphomannomutase
RA 2,123,708 C→T V271V (GTG→GTA cpsB ← mannose‑1‑phosphate guanylyltransferase
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,175,730 Δ1 bp coding (592/1263 nt) gatZ ← putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
RA 2,188,087 T→C T110A (ACG→GCG)  yehA ← putative fimbrial adhesin YehA
RA 2,203,339 C→T R1021C (CGT→TGT)  yehI → DUF4132 domain‑containing protein YehI
RA 2,217,465 C→T A365T (GCG→ACG)  yehY ← glycine betaine ABC transporter membrane subunit YehY
RA 2,222,003 C→T intergenic (‑14/‑182) bglX ← / → dld beta‑D‑glucoside glucohydrolase, periplasmic/quinone‑dependent D‑lactate dehydrogenase
RA 2,233,113 G→A S79N (AGC→AAC)  sanA → DUF218 domain‑containing protein SanA
RA 2,234,387 C→T P119S (CCC→TCC)  preT → dihydropyrimidine dehydrogenase (NAD(+)) subunit PreT
RA 2,249,536 G→A A28V (GCG→GTG)  yeiE ← DNA‑binding transcriptional regulator YeiE
RA 2,255,996 C→T F214F (TTC→TTT yeiL → DNA‑binding transcriptional activator YeiL
RA 2,268,651 C→T P275S (CCC→TCC)  yeiR → zinc‑binding GTPase YeiR
RA 2,272,487 C→T H42Y (CAT→TAT)  yejA → putative oligopeptide ABC transporter periplasmic binding protein YejA
RA 2,288,531 C→T pseudogene (384/2525 nt) yejO ← adhesin‑like autotransporter YejO
RA 2,291,838 C→T A191A (GCG→GCA ccmH ← holocytochrome c synthase CcmH component
RA 2,318,488 C→T A463A (GCG→GCA rcsC ← histidine kinase RcsC
RA 2,333,436 C→T intergenic (‑3639/+900) yfaQ ← / ← yfaT tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT
RA 2,376,046 G→A Y258Y (TAC→TAT menB ← 1,4‑dihydroxy‑2‑naphthoyl‑CoA synthase
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
RA 2,412,154 G→A C147C (TGC→TGT yfbU ← PF03887 family protein YfbU
RA 2,425,264 C→T R218H (CGC→CAC)  hisQ ← lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisQ
RA 2,468,235 C→T P8S (CCT→TCT)  yfdH → bactoprenol glucosyl transferase
RA 2,468,735 C→T N174N (AAC→AAT yfdH → bactoprenol glucosyl transferase
RA 2,483,613 C→T intergenic (‑274/‑116) emrK ← / → evgL tripartite efflux pump membrane fusion protein EmrK/protein EvgL
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
RA 2,492,285 C→T A324T (GCG→ACG)  frc ← formyl‑CoA transferase
RA 2,493,171 C→T W28* (TGG→TGA frc ← formyl‑CoA transferase
RA 2,522,277 G→A G67G (GGC→GGT xapR ← DNA‑binding transcriptional activator XapR
RA 2,555,192 G→A intergenic (‑10/+36) eutR ← / ← eutK putative DNA‑binding transcriptional regulator EutR/putative structural protein, ethanolamine utilization microcompartment
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,574,513 C→T W164* (TGG→TAG)  eutQ ← putative ethanolamine utilization acetate kinase EutQ
RA 2,578,664 C→T intergenic (‑287/‑2) maeB ← / → talA malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))/transaldolase A
JC JC 2,757,507 IS5 (–) +4 bp intergenic (+107/+134) intA → / ← yfjH integrase IntA/uncharacterized protein YfjH
RA 2,760,347 T→C S455P (TCA→CCA)  yfjI → DUF3987 domain‑containing protein YfjI
RA 2,791,765 C→T R165C (CGC→TGC)  gabD → succinate‑semialdehyde dehydrogenase (NADP(+)) GabD
RA 2,821,231 G→A R261C (CGC→TGC)  alaS ← alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
RA 2,837,466 C→T intergenic (‑41/+112) hypF ← / ← hydN carbamoyl‑‑[HypE] ligase/putative electron transport protein HydN
RA 2,850,295 G→A S47S (AGC→AGT hycA ← regulator of the transcriptional regulator FhlA
RA 2,893,820 G→A Q388Q (CAG→CAA ygcN → putative oxidoreductase with FAD/NAD(P)‑binding domain
RA 2,908,575 G→A T364T (ACC→ACT pyrG ← CTP synthetase
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
RA 2,935,250 G→A C339Y (TGC→TAC)  fucP → L‑fucose:H(+) symporter
RA 2,947,250 G→A intergenic (‑72/‑137) mltA ← / → metZ membrane‑bound lytic murein transglycosylase A/tRNA‑Met
RA 2,959,890 C→T A847T (GCA→ACA)  recC ← exodeoxyribonuclease V subunit RecC
RA 2,962,432 G→A intergenic (‑4/+9) recC ← / ← ppdC exodeoxyribonuclease V subunit RecC/prepilin‑type N‑terminal cleavage/methylation domain‑containing protein PpdC
RA 2,995,567 G→A intergenic (+1711/+805) ygeI → / ← insD4 protein YgeI/IS2 insertion element protein InsB
RA 3,081,159 C→T M159I (ATG→ATA tktA ← transketolase 1
RA 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter
RA 3,157,144 C→T A174A (GCC→GCT dkgA → methylglyoxal reductase DkgA
RA 3,167,226 G→A L165F (CTT→TTT)  ygiS ← putative deoxycholate binding periplasmic protein
RA 3,197,524 G→A F706F (TTC→TTT glnE ← fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase
RA 3,197,983 T→C K553K (AAA→AAG glnE ← fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase
JC JC 3,259,884 IS30 (–) +2 bp coding (226‑227/390 nt) tdcF ← putative enamine/imine deaminase
RA 3,324,920 C→T intergenic (‑9/+81) folP ← / ← ftsH dihydropteroate synthase/ATP‑dependent zinc metalloprotease FtsH
RA 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,338,620 G→A A210V (GCC→GTC)  mlaE ← intermembrane phospholipid transport system, integral membrane subunit MlaE
RA 3,345,023 G→A D103N (GAC→AAC)  rpoN → RNA polymerase sigma factor RpoN
RA 3,354,678 G→A intergenic (‑628/‑47) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,372,814 G→A L255L (CTG→TTG)  nanA ← N‑acetylneuraminate lyase
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,390,213 G→A A232T (GCG→ACG)  aaeR → DNA‑binding transcriptional activator AaeR
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
RA 3,469,141 G→A S238F (TCT→TTT)  chiA ← endochitinase
RA 3,513,537 G→A D34D (GAC→GAT trpS ← tryptophan‑‑tRNA ligase
JC JC 3,541,736 IS30 (+) +2 bp coding (1330‑1331/2322 nt) feoB → Fe(2(+)) transporter FeoB
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
JC JC 3,637,435 IS30 (–) +2 bp intergenic (‑25/‑206) yhiN ← / → pitA putative oxidoreductase YhiN/metal phosphate:H(+) symporter PitA
JC JC 3,639,612 IS2 (–) +5 bp coding (105‑109/336 nt) uspB ← universal stress protein B
RA 3,656,868 C→T intergenic (‑128/‑127) hdeA ← / → hdeD periplasmic acid stress chaperone HdeA/acid‑resistance membrane protein
RA 3,682,515 C→T M311I (ATG→ATA dctA ← C4 dicarboxylate/orotate:H(+) symporter
RA 3,687,875 C→T S433S (TCG→TCA bcsC ← cellulose synthase outer membrane channel
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
MC JC 3,708,467 Δ168 bp [proK] [proK]
RA 3,721,168 G→A K163K (AAG→AAA insJ → insertion element IS150 protein InsA
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,751,319 G→A L184L (CTG→TTG)  yiaT ← outer membrane protein YiaT
RA 3,771,979 G→T intergenic (‑235/‑302) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,781,142 G→A intergenic (+125/‑73) lldD → / → trmL L‑lactate dehydrogenase/tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase
RA 3,797,305 G→A V120I (GTT→ATT)  waaL → O‑antigen ligase
RA 3,801,210 G→A H264Y (CAT→TAT)  waaJ ← UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase
RA 3,803,627 G→A P171S (CCC→TCC)  waaB ← UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,818,950 G→A R26H (CGC→CAC)  yicG → PF03458 family inner membrane protein YicG
JC 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,840,891 G→A A283A (GCC→GCT nepI ← purine ribonucleoside exporter
RA 3,869,637 C→A L87L (CTC→CTA cbrA → colicin M resistance protein
RA 3,878,536 G→A Y528Y (TAC→TAT gyrB ← DNA gyrase subunit B
RA 3,897,056 G→A A95T (GCG→ACG)  yieH → 6‑phosphogluconate phosphatase
RA 3,902,040 G→A P61S (CCA→TCA)  bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,931,729 G→A Q138Q (CAG→CAA kup → K(+):H(+) symporter Kup
RA 3,936,638 G→A E121K (GAA→AAA)  rbsB → ribose ABC transporter periplasmic binding protein
RA 3,954,796 G→A G440D (GGC→GAC)  ilvD → dihydroxy‑acid dehydratase
RA 3,958,996 C→T P343S (CCG→TCG)  ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,974,933 G→A R156Q (CGG→CAG)  rffC → RffC
RA 3,978,425 G→A D301N (GAT→AAT)  wecF → TDP‑N‑acetylfucosamine:lipid II N‑acetylfucosaminyltransferase
RA 3,984,686 G→A S441F (TCC→TTC)  aslA ← putative sulfatase AslA
RA 3,991,705 G→A E185K (GAA→AAA)  cyaA → adenylate cyclase
RA 3,991,868 C→T S239F (TCT→TTT)  cyaA → adenylate cyclase
RA 3,993,531 T→C R793R (CGT→CGC cyaA → adenylate cyclase
RA 3,996,049 T→A L156* (TTG→TAG)  yigA → DUF484 domain‑containing protein YigA
RA 4,000,026 G→A G682S (GGT→AGT)  uvrD → DNA helicase II
RA 4,011,802 C→T P243S (CCG→TCG)  bioP → biotin transporter
RA 4,013,387 C→T A112V (GCA→GTA)  metE → cobalamin‑independent homocysteine transmethylase
RA 4,014,791 C→T A580V (GCG→GTG)  metE → cobalamin‑independent homocysteine transmethylase
RA 4,037,203 C→T noncoding (63/77 nt) ileT → tRNA‑Ile
RA 4,073,454 G→A L40L (CTG→TTG)  yihU ← 3‑sulfolactaldehyde reductase
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,093,186 G→A P85S (CCC→TCC)  rhaM ← L‑rhamnose mutarotase
RA 4,094,329 G→A intergenic (‑57/+394) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,100,969 G→A V54I (GTT→ATT)  sodA → superoxide dismutase (Mn)
RA 4,107,225 G→A D253N (GAC→AAC)  fieF → Zn(2(+))/Fe(2(+))/Cd(2(+)) exporter
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,116,633 G→A T197I (ACC→ATC)  glpK ← glycerol kinase
RA 4,116,905 G→A C106C (TGC→TGT glpK ← glycerol kinase
RA 4,127,189 C→T R59R (CGC→CGT rpmE → 50S ribosomal subunit protein L31
RA 4,134,368 C→T T178T (ACC→ACT katG → catalase/hydroperoxidase HPI
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,152,948 C→T A51T (GCT→ACT)  ppc ← phosphoenolpyruvate carboxylase
RA 4,161,248 G→T G61V (GGC→GTC)  fabR → DNA‑binding transcriptional repressor FabR
RA 4,164,123 C→G A162G (GCA→GGA)  btuB → cobalamin outer membrane transporter
RA 4,168,480 T→C intergenic (+33/‑161) gltT → / → rrlB tRNA‑Glu/23S ribosomal RNA
RA 4,179,088 C→T G70G (GGC→GGT rplA → 50S ribosomal subunit protein L1
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,198,552 C→T R279R (CGC→CGT hemE → uroporphyrinogen decarboxylase
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,215,489 G→A Q4Q (CAG→CAA aceB → malate synthase A
RA 4,220,830 G→A A553V (GCC→GTC)  arpA ← regulator of acetyl CoA synthetase
RA 4,223,156 G→A A158V (GCT→GTT)  iclR ← DNA‑binding transcriptional repressor IclR
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,225,937 G→A V704I (GTC→ATC)  metH → cobalamin‑dependent methionine synthase
RA 4,235,646 G→A intergenic (+239/‑260) pgi → / → yjbE glucose‑6‑phosphate isomerase/uncharacterized protein YjbE
RA 4,245,100 G→A intergenic (‑25/+129) malF ← / ← malE maltose ABC transporter membrane subunit MalF/maltose ABC transporter periplasmic binding protein
RA 4,250,215 C→T P221L (CCT→CTT)  malM → maltose regulon periplasmic protein
RA 4,266,517 G→A E246K (GAG→AAG)  alr → alanine racemase 1
RA 4,271,267 G→A Q869* (CAG→TAG)  uvrA ← UvrABC excision nuclease subunit A
RA 4,282,332 (A)5→6 coding (744/1293 nt) yjcF ← pentapeptide repeat‑containing protein YjcF
RA 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,307,123 G→A A226V (GCG→GTG)  alsK ← D‑allose kinase
RA 4,317,383 C→T L171L (CTG→CTA phnL ← methylphosphonate degradation complex subunit PhnL
RA 4,317,851 C→T Q15Q (CAG→CAA phnL ← methylphosphonate degradation complex subunit PhnL
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,336,319 C→T C238Y (TGC→TAC)  adiC ← arginine:agmatine antiporter
RA 4,340,478 G→A T15I (ACC→ATC)  adiA ← arginine decarboxylase, degradative
RA 4,350,035 C→T R543K (AGA→AAA)  dcuS ← sensor histidine kinase DcuS
RA 4,392,997 G→A V357V (GTC→GTT queG ← epoxyqueuosine reductase
RA 4,393,552 G→A F172F (TTC→TTT queG ← epoxyqueuosine reductase
RA 4,394,904 C→T A280V (GCT→GTT)  nnr → NAD(P)HX epimerase/NAD(P)HX dehydratase
RA 4,396,373 C→T T103T (ACC→ACT amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,396,772 T→C F236F (TTT→TTC amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,401,601 C→T I310I (ATC→ATT hflX → ribosome rescue factor HflX
RA 4,415,033 C→T R253R (CGC→CGT aidB → putative acyl‑CoA dehydrogenase AidB
RA 4,425,587 G→A G23E (GGA→GAA)  priB → primosomal replication protein N
RA 4,425,688 C→A H57N (CAC→AAC)  priB → primosomal replication protein N
RA 4,425,696 C→A N59K (AAC→AAA priB → primosomal replication protein N
RA 4,458,044 C→T A29V (GCC→GTC)  tldE → metalloprotease subunit TldE
RA 4,460,372 C→T intergenic (‑8/+150) nrdG ← / ← nrdD anaerobic ribonucleoside‑triphosphate reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase
RA 4,463,287 C→T G475S (GGC→AGC)  treC ← trehalose‑6‑phosphate hydrolase
RA 4,465,986 C→T Q65Q (CAG→CAA treB ← trehalose‑specific PTS enzyme IIBC component
RA 4,466,307 G→A L314L (CTG→TTG)  treR ← DNA‑binding transcriptional repressor TreR
RA 4,486,647 C→T L144L (CTG→TTG)  lptF → lipopolysaccharide transport system protein LptF
RA 4,489,184 C→T G294S (GGC→AGC)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,496,010 C→T G67E (GGG→GAG)  ahr ← NADPH‑dependent aldehyde reductase Ahr
RA 4,502,076 G→A intergenic (+487/+27) yjgZ → / ← insG uncharacterized protein YjgZ/IS4 family putative transposase
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,512,933 C→T L159L (CTG→CTA fecC ← ferric citrate ABC transporter membrane subunit FecC
RA 4,521,147 C→T G292R (GGG→AGG)  yjhF ← putative transporter YjhF
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
JC JC 4,539,115 IS5 (–) +4 bp coding (384‑387/717 nt) nanC ← N‑acetylneuraminate outer membrane channel
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,548,466 C→T A61V (GCC→GTC)  fimG → type 1 fimbriae minor subunit FimG
RA 4,548,824 C→T T6I (ACC→ATC)  fimH → type 1 fimbriae D‑mannose specific adhesin
RA 4,551,386 C→T intergenic (‑90/‑250) gntP ← / → uxuA fructuronate transporter/D‑mannonate dehydratase
RA 4,554,575 C→T intergenic (+214/‑1) uxuB → / → uxuR D‑mannonate oxidoreductase/DNA‑binding transcriptional repressor UxuR
RA 4,558,283 C→T D3N (GAC→AAC)  hypT ← DNA‑binding transcriptional dual regulator HypT
RA 4,563,755 C→T W57* (TGG→TGA ytiA ← uncharacterized protein YtiA
RA 4,607,607 C→T D32N (GAC→AAC)  rsmC ← 16S rRNA m(2)G1207 methyltransferase
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD
JC JC 4,640,418 IS30 (–) +2 bp coding (17‑18/141 nt) yjjY → uncharacterized protein YjjY

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 736704 737223 520 41 [40] [40] 42 ybfQ/ybfD inactive transposase YbfQ/H repeat‑associated putative transposase YbfD
* * ÷ NC_000913 1196277 1211459 15183 42 [40] [38] 43 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270 776 127 [0] [2] 137 insB5–insA5 insB5,insA5
* * ÷ NC_000913 2726233–2729242 2729426 185–3194 41 [40] [40] 42 rrlG–[gltW] rrlG,[gltW]
* * ÷ NC_000913 3423773–3424234 3426628–3424239 6–2856 41 [40] [40] 41 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3943770–3945357 3945357 1–1588 41 [38] [40] 41 rrlC 23S ribosomal RNA
* * ÷ NC_000913 4035337–4036916 4036916 1–1580 42 [40] [39] 42 [rrsA] [rrsA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 737973 =151 (1.300)88 (0.830)
+25 bp
56/548 0.6 39.9% intergenic (+1274/‑119) ybfQ/ybfD inactive transposase YbfQ/H repeat‑associated putative transposase YbfD
?NC_000913 = 3624614 138 (1.190)coding (237/1137 nt) yhhI putative transposase
* ? NC_000913 = 1397238NA (NA)127 (1.090) 78/598 0.2 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1469296 =NA (NA)37 (0.320) 33/592 2.8 46.2% noncoding (1/1221 nt) IS30 repeat region
?NC_000913 = 1910137 43 (0.370)intergenic (‑38/‑139) kdgR/yebQ DNA‑binding transcriptional repressor KdgR/putative transporter YebQ
* ? NC_000913 = 1470516NA (NA)70 (0.610) 54/596 1.0 61.4% noncoding (1221/1221 nt) IS30 repeat region
?NC_000913 1910136 = 44 (0.380)intergenic (‑37/‑140) kdgR/yebQ DNA‑binding transcriptional repressor KdgR/putative transporter YebQ
* ? NC_000913 1979271 =2 (0.020)135 (1.160) 91/598 0.1 98.5% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL