mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.39.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 42,108 | G→A | intergenic (‑177/‑295) | caiT ← / → fixA | L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA |
| RA | 229,552 | C→T | S129F (TCC→TTC) | dkgB → | methylglyoxal reductase DkgB |
| JC JC | 258,921 | IS1 (+) +9 bp | pseudogene (246‑254/331 nt) | crl → | RNA polymerase holoenzyme assembly factor Crl |
| RA | 284,535 | T→G | A445A (GCT→GCG) | yagF → | D‑xylonate dehydratase |
| RA | 696,470 | C→T | noncoding (35/75 nt) | glnX ← | tRNA‑Gln |
| RA | 790,832 | Δ1 bp | coding (198/1047 nt) | galT ← | galactose‑1‑phosphate uridylyltransferase |
| RA | 1,001,251 | A→C | Q679P (CAA→CCA) | elfC → | putative fimbrial usher protein ElfC |
| RA | 1,148,972 | C→T | Q72* (CAG→TAG) | fabH → | 3‑oxoacyl‑[acyl carrier protein] synthase 3 |
| RA | 1,169,836 | A→G | L180P (CTG→CCG) | ldtC ← | L,D‑transpeptidase LdtC |
| RA | 1,189,980 | A→G | T156T (ACT→ACC) | phoP ← | DNA‑binding transcriptional dual regulator PhoP |
| RA | 1,196,220 | C→T | H366H (CAC→CAT) | icd → | isocitrate dehydrogenase |
| RA | 1,196,232 | C→T | T370T (ACC→ACT) | icd → | isocitrate dehydrogenase |
| RA | 1,196,245 | T→C | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
| RA | 1,196,247 | A→G | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
| RA | 1,196,277 | C→T | N385N (AAC→AAT) | icd → | isocitrate dehydrogenase |
| RA | 1,196,280 | G→C | A386A (GCG→GCC) | icd → | isocitrate dehydrogenase |
| RA | 1,196,283 | A→G | K387K (AAA→AAG) | icd → | isocitrate dehydrogenase |
| RA | 1,196,292 | C→T | T390T (ACC→ACT) | icd → | isocitrate dehydrogenase |
| RA | 1,287,040 | G→A | intergenic (+56/+26) | ychS → / ← rttR | putative uncharacterized protein YchS/small RNA RttR |
| JC | 1,301,457 | (CTGGGATAAT)1→2 | coding (276/1632 nt) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
| RA | 1,301,992 | A→T | N271Y (AAT→TAT) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
| RA | 1,306,736 | T→G | S325A (TCC→GCC) | oppF → | murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF |
| RA | 1,336,674 | G→A | G282R (GGG→AGG) | acnA → | aconitate hydratase 1 |
| RA | 1,337,394 | A→G | S522G (AGC→GGC) | acnA → | aconitate hydratase 1 |
| RA | 1,339,315 | C→T | intergenic (‑155/‑15) | ribA ← / → pgpB | GTP cyclohydrolase 2/phosphatidylglycerophosphatase B |
| RA | 1,358,859 | T→C | Y110C (TAT→TGT) | puuP ← | putrescine:H(+) symporter PuuP |
| RA | 1,643,679 | A→T | L209Q (CTG→CAG) | ydfU ← | protein YdfU |
| RA | 1,652,331 | T→C | intergenic (+2346/+397) | ydfD → / ← ynfP | lysis protein/protein YnfP |
| RA | 1,829,887 | G→A | A293A (GCC→GCT) | astA ← | arginine N‑succinyltransferase |
| RA | 1,894,839 | T→C | L12P (CTC→CCC) | pabB → | aminodeoxychorismate synthase subunit 1 |
| RA | 1,948,964 | G→A | A520V (GCT→GTT) | aspS ← | aspartate‑‑tRNA ligase |
| RA | 2,040,433 | C→A | A319D (GCC→GAC) | msrP → | protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP |
| RA | 2,042,171 | G→A | intergenic (+146/‑197) | zinT → / → yodB | metal‑binding protein ZinT/putative cytochrome b561 YodB |
| RA | 2,044,642 | T→C | intergenic (+18/‑296) | asnT → / → yeeJ | tRNA‑Asn/inverse autotransporter adhesin |
| RA | 2,111,259 | Δ1 bp | coding (718/900 nt) | rfbD ← | dTDP‑4‑dehydrorhamnose reductase |
| RA | 2,173,363 | Δ2 bp | intergenic (‑490/+918) | gatD ← / ← gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| RA | 2,173,448 | C→T | intergenic (‑575/+834) | gatD ← / ← gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| RA | 2,339,161 | T→C | D87G (GAC→GGC) | gyrA ← | DNA gyrase subunit A |
| JC JC | 2,375,828 | IS186 (+) +4 bp | coding (132‑135/963 nt) | menC ← | o‑succinylbenzoate synthase |
| JC JC | 2,406,612 | IS2 (–) +5 bp | coding (26‑30/939 nt) | lrhA ← | DNA‑binding transcriptional dual regulator LrhA |
| RA | 2,413,411 | C→T | intergenic (‑279/‑59) | yfbV ← / → ackA | PF04217 family membrane protein YfbV/acetate kinase |
| RA | 2,414,829 | A→G | E28G (GAA→GGA) | pta → | phosphate acetyltransferase |
| JC JC | 2,468,298 | IS3 (+) +3 bp | coding (85‑87/921 nt) | yfdH → | bactoprenol glucosyl transferase |
| RA | 2,587,112 | C→T | A461V (GCA→GTA) | narQ → | sensor histidine kinase NarQ |
| RA | 2,599,947 | G→T | L14F (TTG→TTT) | gcvR → | putative transcriptional regulator GcvR |
| RA | 2,612,191 | G→T | G98* (GGA→TGA) | hyfR → | DNA‑binding transcriptional activator HyfR |
| MC JC | 2,793,101 | Δ6 bp | coding (367‑372/1281 nt) | gabT → | 4‑aminobutyrate aminotransferase GabT |
| RA | 2,867,455 | G→A | Q33* (CAG→TAG) | rpoS ← | RNA polymerase sigma factor RpoS |
| MC JC | 2,906,529 | Δ12 bp | coding (819‑830/873 nt) | ygcG → | TPM domain‑containing protein YgcG |
| RA | 2,926,772 | C→A | G155G (GGC→GGA) | ppnN → | nucleotide 5'‑monophosphate nucleosidase |
| RA | 3,152,494 | G→T | A87S (GCG→TCG) | metC → | cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase |
| RA | 3,207,549 | Δ1 bp | coding (179/606 nt) | ttdB → | L(+)‑tartrate dehydratase subunit beta |
| RA | 3,329,743 | G→T | D261Y (GAT→TAT) | dacB → | peptidoglycan DD‑endopeptidase DacB |
| JC JC | 3,350,556 | IS1 (–) +8 bp | intergenic (‑97/‑14) | elbB ← / → arcZ | low activity glyoxalase ElbB/small regulatory RNA ArcZ |
| RA | 3,388,041 | T→G | T50P (ACG→CCG) | aaeB ← | aromatic carboxylic acid efflux pump subunit AaeB |
| JC JC | 3,429,988 | IS3 (–) +3 bp | coding (34‑36/258 nt) | yrdB ← | DUF1488 domain‑containing protein YrdB |
| RA | 3,560,443 | C→G | intergenic (+577/+179) | rtcR → / ← glpG | DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG |
| RA | 3,560,455 | +G | intergenic (+589/+167) | rtcR → / ← glpG | DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG |
| RA | 3,597,677 | T→C | intergenic (‑117/‑307) | livK ← / → panZ | L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein/PanD maturation factor |
| RA | 3,689,145 | G→T | T10K (ACG→AAG) | bcsC ← | cellulose synthase outer membrane channel |
| RA | 3,696,157 | G→T | P10H (CCT→CAT) | yhjR ← | PF10945 family protein YhjR |
| RA | 3,703,531 | G→T | Y106* (TAC→TAA) | dppD ← | dipeptide ABC transporter ATP binding subunit DppD |
| RA | 3,707,883 | G→A | intergenic (‑178/+733) | dppA ← / ← proK | dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro |
| RA | 3,707,947 | C→A | intergenic (‑242/+669) | dppA ← / ← proK | dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro |
| RA | 3,725,176 | T→G | E48A (GAG→GCG) | glyQ ← | glycine‑‑tRNA ligase subunit alpha |
| RA | 3,815,883 | +C | coding (667/687 nt) | rph ← | truncated RNase PH |
| JC | 4,123,883 | Δ4 bp | coding (571‑574/1026 nt) | cytR ← | DNA‑binding transcriptional repressor CytR |
| RA | 4,128,813 | Δ2 bp | coding (142‑143/1161 nt) | metB → | O‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase |
| RA | 4,223,638 | T→C | intergenic (‑10/‑190) | iclR ← / → metH | DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase |
| RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| RA | 4,359,314 | G→A | Q240* (CAG→TAG) | cadB ← | lysine:cadaverine antiporter |
| RA | 4,380,292 | 2 bp→A | coding (225‑226/735 nt) | frdB ← | fumarate reductase iron‑sulfur protein |
| RA | 4,392,997 | G→A | V357V (GTC→GTT) | queG ← | epoxyqueuosine reductase |
| RA | 4,393,552 | G→A | F172F (TTC→TTT) | queG ← | epoxyqueuosine reductase |
| RA | 4,396,373 | C→T | T103T (ACC→ACT) | amiB → | N‑acetylmuramoyl‑L‑alanine amidase B |
| RA | 4,396,772 | T→C | F236F (TTT→TTC) | amiB → | N‑acetylmuramoyl‑L‑alanine amidase B |
| RA | 4,425,688 | C→A | H57N (CAC→AAC) | priB → | primosomal replication protein N |
| RA | 4,425,696 | C→A | N59K (AAC→AAA) | priB → | primosomal replication protein N |
| RA | 4,436,956 | 2 bp→TT | coding (202‑203/741 nt) | cysQ → | 3'(2'),5'‑bisphosphate nucleotidase |
| RA | 4,510,238 | T→G | intergenic (+445/+452) | insO → / ← fecE | IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit |
| RA | 4,532,409 | T→G | intergenic (‑758/+28) | sgcX ← / ← yjhP | putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP |
| JC JC | 4,542,150 | IS1 (+) +9 bp | coding (114‑122/597 nt) | fimE → | regulator for fimA |
| RA | 4,546,601 | C→T | I502I (ATC→ATT) | fimD → | type I fimbriae usher protein |
| RA | 4,549,037 | T→C | V77A (GTC→GCC) | fimH → | type 1 fimbriae D‑mannose specific adhesin |
| RA | 4,578,548 | G→A | Q264* (CAG→TAG) Q264* (CAG→TAG) |
mcrB ← mcrB ← |
5‑methylcytosine‑specific restriction enzyme subunit McrB 5‑methylcytosine‑specific restriction enzyme subunit McrB |
| JC JC | 4,580,649 | IS5 (–) +4 bp | coding (811‑814/1395 nt) | hsdS ← | type I restriction enzyme EcoKI specificity subunit |
| RA | 4,636,925 | G→C | R77P (CGC→CCC) | creC → | sensory histidine kinase CreC |
| RA | 4,638,240 | G→A | L21L (TTG→TTA) | creD → | putative inner membrane protein CreD |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 257908–258675 | 258676 | 2–769 | 28 [0] | [0] 2 | insB9–[crl] | insB9,insA9,[crl] |
| * | * | ÷ | NC_000913 | 323451 | 337785 | 14335 | 45 [0] | [1] 2 | [ykgF]–[yahF] | [ykgF],ykgG,ykgH,betA,betB,betI,betT,yahV,pdeL,yahB,yahC,yahD,yahE,[yahF] |
| * | * | ÷ | NC_000913 | 337802 | 339567 | 1766 | 2 [1] | [1] 2 | [yahF]–[yahG] | [yahF],[yahG] |
| * | * | ÷ | NC_000913 | 339645 | 362203 | 22559 | 2 [1] | [0] 37 | [yahG]–[lacY] | [yahG],yahH,yahI,yahJ,yahK,yahL,yahM,yahN,yahO,prpR,prpB,prpC,prpD,prpE,codB,codA,cynR,cynT,cynS,cynX,lacA,[lacY] |
| * | * | ÷ | NC_000913 | 1196375 | 1211412 | 15038 | 2 [0] | [0] 2 | C0293–mcrA | C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA |
| * | * | ÷ | NC_000913 | 1906879 | 1907878 | 1000 | 2 [0] | [0] 17 | [rlmA]–yobF | [rlmA],cspC,yobF |
| * | * | ÷ | NC_000913 | 1979272 | 1979278 | 7 | 3 [1] | [0] 13 | insA5/uspC | IS1 family protein InsA/universal stress protein C |
| * | * | ÷ | NC_000913 | 2558699 | 2565484 | 6786 | 42 [0] | [1] 3 | intZ–[eutA] | intZ,yffL,yffM,yffN,yffO,yffP,yffQ,yffR,yffS,[eutA] |
| * | * | ÷ | NC_000913 | 2822836 | 2824403 | 1568 | 2 [0] | [1] 2 | [recA]–pncC | [recA],pncC |
| * | * | ÷ | NC_000913 | 2824498 | 2829585 | 5088 | 2 [1] | [0] 4 | [mltB]–[srlR] | [mltB],srlA,srlE,srlB,srlD,gutM,[srlR] |
| * | * | ÷ | NC_000913 | 3423900–3424234 | 3424410–3424238 | 5–511 | 2 [1] | [1] 2 | rrlD | 23S ribosomal RNA |
| * | * | ÷ | NC_000913 | 4166886–4168220 | 4168480 | 261–1595 | 2 [1] | [1] 2 | [rrsB]–gltT | [rrsB],gltT |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 257907 | 0 (0.000) | 28 (0.650) | 27/466 | 0.5 | 100% | intergenic (+8/+16) | crl/insB9 | RNA polymerase holoenzyme assembly factor Crl/IS1 family transposase B |
| ? | NC_000913 | 258684 = | 0 (0.000) | pseudogene (9/331 nt) | crl | RNA polymerase holoenzyme assembly factor Crl | |||||
| * | ? | NC_000913 | 257908 = | NA (NA) | 45 (1.010) | 43/482 | 0.2 | 100% | noncoding (768/768 nt) | IS1 | repeat region |
| ? | NC_000913 | = 323450 | 0 (0.000) | coding (1113/1428 nt) | ykgF | putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain | |||||
| * | ? | NC_000913 | = 258675 | NA (NA) | 37 (0.830) | 35/482 | 0.3 | 100% | noncoding (1/768 nt) | IS1 | repeat region |
| ? | NC_000913 | 362204 = | 0 (0.000) | coding (976/1254 nt) | lacY | lactose permease | |||||
| * | ? | NC_000913 | = 291398 | NA (NA) | 4 (0.090) +CCC |
4/476 | 2.3 | 100% | noncoding (4/768 nt) | IS1 | repeat region |
| ? | NC_000913 | = 4542158 | 0 (0.000) | coding (122/597 nt) | fimE | regulator for fimA | |||||
| * | ? | NC_000913 | = 566774 | 56 (1.250) | 15 (0.340) | 5/478 | 2.1 | 23.7% | pseudogene (91/93 nt) | renD | protein RenD |
| ? | NC_000913 | 568036 = | 41 (0.930) | pseudogene (2/213 nt) | renD | protein RenD | |||||
| * | ? | NC_000913 | = 1299498 | 0 (0.000) | 11 (0.250) | 9/474 | 1.5 | 100% | intergenic (+253/‑69) | ychE/insH21 | MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator |
| ? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+151/‑484) | insH21/oppA | IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein | |||||
| * | ? | NC_000913 | = 1906877 | 0 (0.000) | 37 (0.830) | 36/480 | 0.3 | 100% | coding (184/810 nt) | rlmA | 23S rRNA m(1)G745 methyltransferase |
| ? | NC_000913 | = 2290113 | NA (NA) | noncoding (1/1195 nt) | IS5 | repeat region | |||||
| * | ? | NC_000913 | 1907879 = | 0 (0.000) | 17 (0.380) | 16/482 | 1.0 | 100% | intergenic (‑288/+382) | yobF/yebO | DUF2527 domain‑containing protein YobF/uncharacterized protein YebO |
| ? | NC_000913 | 2101749 = | NA (NA) | noncoding (1195/1195 nt) | IS5 | repeat region | |||||
| * | ? | NC_000913 | = 1978502 | 12 (0.270) | 13 (0.300) | 13/466 | 1.1 | 69.2% | intergenic (‑305/+16) | flhD/insB5 | DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB |
| ? | NC_000913 | 1979279 = | 0 (0.000) | intergenic (‑64/‑474) | insA5/uspC | IS1 family protein InsA/universal stress protein C | |||||
| * | ? | NC_000913 | = 2558698 | 0 (0.000) | 41 (0.950) | 39/466 | 0.2 | 100% | intergenic (‑19/‑160) | eutB/intZ | ethanolamine ammonia‑lyase subunit alpha/putative phage integrase IntZ |
| ? | NC_000913 | 2565489 = | 0 (0.000) | coding (1396/1404 nt) | eutA | ethanolamine ammonia‑lyase reactivase EutA | |||||