| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,128,813 | Δ2 bp | coding (142‑143/1161 nt) | metB → | O‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,128,811 | 0 | C | . | 98.4% | 289.5 / ‑1.7 | 64 | coding (140/1161 nt) | metB | O‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base . (36/27); total (37/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-01 | |||||||||||
| * | NC_000913 | 4,128,812 | 0 | G | . | 98.4% | 289.7 / ‑3.6 | 64 | coding (141/1161 nt) | metB | O‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (36/27); total (37/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGTTGTTTGTTGATCAAGGCGATGAACA > NC_000913/4128561‑4129054 || ctccaTCTATACGCAAAGCAGTTTCGATGCCCAGCTGGATTGACGTCCATTAACACAATGCTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACCCGTAAACCGGCCACCCTCGCAGTGCGTAGCGGGTTAACTGACGACGCACAGTATGGTTGCGTTGTACCACCGATCCATCTTTGCACCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtc > 1:50282/5‑251 (MQ=255) cAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtcgt > 2:147227/1‑250 (MQ=255) tataCGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtcgtcgcgg > 2:336229/1‑245 (MQ=255) ataCGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtcgtcg < 1:185671/247‑4 (MQ=255) agaagTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAAc < 1:170416/251‑1 (MQ=255) gaagTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAAcg > 1:44277/1‑251 (MQ=255) aagTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAACCCCAGGAAACTTCATCACACGACGAGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAAAGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATATTTCCAGCACC‑TATAACTTTACAGGATTTAATGAACCGCGCGCGCCTGATTACT‑‑CGTCGCGGCAACCCAAcgc > 1:312314/1‑251 (MQ=255) aGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGAtg > 1:391626/1‑251 (MQ=255) aGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGAtg > 2:414336/1‑251 (MQ=255) aGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTCTGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTc > 2:337391/1‑249 (MQ=255) tGACGTACATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTAGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCt > 1:440122/1‑251 (MQ=255) gTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGc > 1:22630/1‑250 (MQ=255) ttAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAACCAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCCGCGTGCGCTGGCAGAACt > 1:439109/1‑251 (MQ=255) tAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTg > 2:235830/1‑251 (MQ=255) ttACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGTGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGgtggtg < 1:185940/250‑1 (MQ=255) aCTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGtggtgctg < 2:137442/251‑1 (MQ=255) ctctGGTGCCTGACCTTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGtggtgctgg > 1:368891/1‑251 (MQ=255) tGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGtggtgctggtgc > 2:350822/1‑251 (MQ=255) ggTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAATGtggtgctggt < 1:357219/248‑1 (MQ=255) ggTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCa < 1:187792/251‑1 (MQ=255) gTGCCTGACAGTTCACCGACAAAGCCCAGGGAACGTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAg < 1:342316/251‑1 (MQ=255) gCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGGGCAGt > 2:90008/1‑250 (MQ=255) aCATTTCCCAGACAAAGACCAGGGAACTTCATCACATGACGCGTAAACAGGCCCCCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAAGTGGTGCTGGTGCAGtacttac > 2:153047/1‑251 (MQ=255) aCATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGtacttac < 1:235830/251‑1 (MQ=255) aTTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGtacttact > 1:403287/1‑250 (MQ=255) cACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATa < 2:120512/250‑1 (MQ=255) cGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGACCAGTATGGATGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGACGCGGCAACCCAACGCGCGATGAGGTTCAGCGTGCTCTGGCAGAACTGGAAGGTGGTGCTGGAGCAGAACTTACTAATACCgg > 2:40567/1‑251 (MQ=255) cGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGTCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGACCTGGAAGGTGGTGCTGGTGCAGTCCTTCCTAATACCgg > 2:405/1‑251 (MQ=255) gACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGc > 1:6117/1‑251 (MQ=255) aaaGTCCAGGGAACTTCATCCCATGACGCGTAAACAGGCCACCATCGAAGTGCGTAGCGGGTTAAATGACGACGAACAGGGTGGTTGCGCTGTCCCACCGCTCAATCTTTCCAG‑ACCGTATAACTTTAACGGACTTAATGAACCGCGAGCGCATGATTACT‑‑AGTCGCGGAAACCCAAAGCGCGCTGTGGTTCAGCGTACGCTGGCAGAAATGGAAGGTGGTGCTGGTGCAGAACATACTCATACAGGcctg > 1:393045/1‑248 (MQ=255) atcccATGGAACTTCATCGCATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAAGGACGACGAACAGTATTGTCGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTGTACCGGATCTAATGAACCGCGCGCTCAGGATTACT‑‑CGTCGCGGCATCCCATCGCGCGAGGTGGTTCAGCGTGCGCTGGCAGAACCGGAAGGTGGTGCTGGTGCAGTGTTTCCTAATACCTGCATGTc < 2:50282/249‑1 (MQ=255) aGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTc < 2:402936/251‑1 (MQ=255) aGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTc > 1:367091/1‑251 (MQ=255) cAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCATCCCAACGCGCGATGTGGTTCAGCGGGCGCTGGCATAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGTATGGCcgcg < 2:443050/251‑1 (MQ=255) ggAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGAtt < 2:53756/251‑1 (MQ=255) gAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAAGACCGGCATGTCCGCGATTc < 2:44277/251‑1 (MQ=255) ttCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGAATGTCCGCGATTCACCt > 2:366672/1‑251 (MQ=255) tcatcaCATGACGCGTAAACAAGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCCCCGATCCATCTTTCCAGCACC‑TATAACTGTACCGGATTTAATGAACCGCGCGCGCATGATGACT‑‑CGTCGCGGTAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCt < 2:105021/250‑1 (MQ=255) caTGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGAGGTTCAACTGGTAACg > 2:140566/1‑251 (MQ=255) gACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGAc < 1:414336/250‑1 (MQ=255) ggTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGcgcc > 2:172693/1‑251 (MQ=255) gTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGcgccg > 1:278736/1‑251 (MQ=255) ttAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGcgccgc > 1:308796/1‑251 (MQ=255) gacgaACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCt < 1:147227/251‑1 (MQ=255) cgaACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTAc < 1:177589/251‑1 (MQ=255) aGTATGGTTGCGTTGGCCCACCGATGCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGGATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTAcggcgg < 2:391626/251‑1 (MQ=255) tATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGt > 1:356409/1‑250 (MQ=255) aTGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTa < 2:368891/250‑1 (MQ=255) tGCGTTGTCCCACCGATCCATCTTTTCAGCACC‑TATAACTTTACCGGGTTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGCAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACCGATAcggcgggaactca > 2:431273/1‑243 (MQ=255) tGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGCTGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGGAGCTATc > 1:196794/1‑251 (MQ=255) cGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGc > 1:286459/1‑251 (MQ=255) tGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGccattccccg > 2:232378/1‑242 (MQ=255) tCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCAAGACTGCTAcggcggcagatctcgcccgtt > 1:459137/1‑236 (MQ=255) ccaaCCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGtt < 2:403287/247‑1 (MQ=255) cACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGC‑‑CGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCAGCGATTCACCTGGTAACGACCGTCTTTTTGAAAACTGGCGATCTGCTGGTTGCGCCGCACGACTGCTAcggcggcaccacaaccctatttac > 2:88740/1‑233 (MQ=255) cACCGATCCATATTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCAAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTAACCTGGTAAAGCCCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTAcggcgggagacatcgaccgtttg > 2:419958/1‑233 (MQ=255) gATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAAACTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGAAGCTATCGCCTGTTCGAccgg > 2:88480/1‑248 (MQ=255) gATCCATCTTTCCAGCACC‑TATAACGTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGt < 2:293691/251‑1 (MQ=255) ccAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAAcg < 1:350822/251‑1 (MQ=255) aCC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGtt < 1:419267/251‑1 (MQ=255) cc‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGGTCGGCAAGCTGGCGGAACACGGTTg > 1:122189/1‑251 (MQ=255) ataACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCCATCGCCTGTTTGGCCGTCCGGCGAAACGCGGTGGCtt > 1:443741/1‑250 (MQ=255) aaCTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTTGACAGTCTGGGGAAACGCGGTTGCTATc > 1:176709/1‑251 (MQ=255) aCCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCgtgt < 2:356409/251‑1 (MQ=255) ccGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGCTCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTTGCGAAACGCGGTTGCTATcgcggggt > 1:51843/1‑247 (MQ=255) aaTGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGTtgtttgtt < 1:405/250‑1 (MQ=255) gcgcgcgcATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGTTGTTTGTTGATCAAGGc < 1:230336/251‑1 (MQ=255) aTGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACGTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGTTGTTTGTTGATCAAGGCGATGAACa < 2:352707/251‑1 (MQ=255) || CTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACC‑TATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGTTGTTTGTTGATCAAGGCGATGAACA > NC_000913/4128561‑4129054 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |