Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,703,531	G→T	Y106* (TAC→TAA)	dppD ←	dipeptide ABC transporter ATP binding subunit DppD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,703,531	0	G	T	100.0%	197.2 / NA	67	Y106* (TAC→TAA)	dppD	dipeptide ABC transporter ATP binding subunit DppD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/42);  total (25/42)

AATGGTCACGTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTGTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGT  >  NC_000913/3703285‑3703766                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               aaTGGTCACGTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGc                                                                                                                                                                                                                                            <  1:322065/251‑1 (MQ=255)     gTCACGTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAAGCGCTCGCTGACGGCGGGTGCTTTT‑GTTGCCGCCCTGATGC‑GCCTTAATCGCTTCCATAATCTGGAAGCCCTC‑GGTTTAGCAC‑gg                                                                                                                                                                                                                                       <  2:160750/251‑1 (MQ=255)          gTCCAGCGCGATGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACATTT‑TTTGCCGCCCTGATGC‑CCCTTCATCGCTTCCCGAATCTGGAAACCCCC‑GGGTTAGCAC‑ggggta                                                                                                                                                                                                                                   >  2:404638/1‑247 (MQ=255)              aGCGCGGTGTGCCGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACTTCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGGCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCTCCCTTATGC‑ACCTTAATCGCTTCCATAAGCGGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCt                                                                                                                                                                                                                              <  2:421866/251‑1 (MQ=255)                   ggtggtCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTTGTT‑CCGCCCTGATGC‑ACCCTAAACGCTTCCCTAATCTTGAAACCCAC‑GGGTTTGCAA‑GGGTTAAGGCTGGTc                                                                                                                                                                                                                          >  2:465582/1‑250 (MQ=255)                    gtggtCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCAt                                                                                                                                                                                                                        <  1:304778/251‑1 (MQ=255)                               aTCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGGAACCCAC‑CGTTTAGCAC‑GGGTTAAGGCTGGGCATCGGGTCCTggg                                                                                                                                                                                                             >  1:187750/1‑250 (MQ=255)                                         agcagTTTTGGCCGACAGGCAATCGCCATGGCGATCATCCCGCGCTGGCTCATGCCGCCGGAACGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCACTCGCTCGCTGACGGCGGGGACCTTT‑GTTGCCCCCCTGCTCC‑ACCTTCATCACTTTCCTAATCTCGAAACCCAC‑GGTTTAGCAC‑GGGGTAAGGCTGGTCATCggggccctgcagaccatctc                                                                                                                                                                                                   >  2:20919/1‑234 (MQ=255)                                                  ggCCGACAGGCAATCGCCAGGGCGATCATCCCGCGCTGGCTCATGCCGACGGAAAGCGGAGGCGGGTAAACATCCCGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCGGGAAGATGATCGC‑CACTTc                                                                                                                                                                                            <  2:432073/248‑1 (MQ=255)                                                       aCAGGCAATAGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGcaccca                                                                                                                                                                                    <  2:258527/251‑1 (MQ=255)                                                         aGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTCCTTTT‑TTTGGCGCCCTGATGC‑ACATTAATCGCTTCCATAATTTGGGAACCCAC‑GGTTTAGAAC‑GGGTTACGGCTGGTCATAGGGACCTGGAAGATCATCGC‑CACTTCGGcacccaac                                                                                                                                                                                  >  1:313931/1‑249 (MQ=255)                                                              aTCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGtt                                                                                                                                                                             <  1:337392/251‑1 (MQ=255)                                                               gcGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTg                                                                                                                                                                            <  2:295593/250‑1 (MQ=255)                                                                 gCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTgcg                                                                                                                                                                          <  1:97194/251‑1 (MQ=255)                                                                   cATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGCCGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTTT‑GTTGCCGCCCCGATGC‑ACCTTAAACGCTTCCCTAATCTGGAAACCCCC‑GGGTTTTCAC‑GGGTTTAGGCTGGTCCTCGGGTCCTGGGAGATCATCCC‑CCCTTCCGcacccccccgggtggggg                                                                                                                                                                        >  2:444678/1‑238 (MQ=255)                                                                      ggCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGAGGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGct                                                                                                                                                                     <  2:355221/251‑1 (MQ=255)                                                                               caCGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTTT‑TTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCCTAATCTGGAAACCCCC‑GGTTTAGCCC‑GGGGTAAGGCTGGTCATCGGGTCCTGGGAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCtt                                                                                                                                                            >  1:68233/1‑250 (MQ=255)                                                                                aCGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGtt                                                                                                                                                                                                                                    <  2:132028/179‑1 (MQ=255)                                                                                aCGCGCTGGCTCATGCCGACGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑ggggt                                                                                                                                                                                                                                    >  1:132028/1‑177 (MQ=255)                                                                                  gcgcTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTGT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTGCTgaga                                                                                                                                                         <  2:85602/251‑1 (MQ=255)                                                                                       ggCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCTCCAGGTTGCGGCGCTCTTTTTCGGAGATAc                                                                                                                                                      <  2:68233/249‑1 (MQ=255)                                                                                           aagtccgccgGAAAGCTGAAGAGGGTAAACATCCAGACGAGATGGCGGATCGTGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCTCCCTGATGC‑ACCTTAATCGCTTCCATAATCTTGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCGGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTTCGGCGCTCTTTGGCTTTGATACGCTGCa                                                                                                                                                <  2:37385/247‑1 (MQ=255)                                                                                             tGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTTT‑GTTGCCTCCCTGAATC‑ACCCTAATCCCTTCCATAATCTGGAAAACCCC‑GGCTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGaa                                                                                                                                                                                                         >  1:449867/1‑192 (MQ=255)                                                                                             tGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATGCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GTTTTAGCAC‑GGGGTAAGGCTGGTCATCGGGTCCTGGAAGat                                                                                                                                                                                                         <  2:449867/193‑1 (MQ=255)                                                                                                 gccgGATAACTGATTCGTGTAAACATCCTGGCGCGATGCCGGATCGGGTATACCGCCCTTATTCAGCAGGTAAATCGCTCGCTTACGGCTGCTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGTTTCCATAATCAGGAAACCCAC‑GGTTTATCTC‑GGGTTAAGTCTGGTCATCGGGTCCTGGAAGATCATCGCTCA‑TTCGGCACCCACCAGGTTGCTGCGCTCTCGGTCTGAGATACGCTGCAAAACCt                                                                                                                                          <  2:157073/251‑1 (MQ=255)                                                                                                 gccgGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTTT‑GTTGCCGCCCCGATGC‑ACCTTAATCGATTCCATACTCTGGGAACCCACCGGTTTA‑CAC‑GGGGTAAGGCTGGTCATCGGGGCCTGGAAGATCATCGC‑CACTTCCGCACCCCCCACGTTGTGGCGCTCTTTTTCTGAGATACGCTGCCAATccc                                                                                                                                          >  2:464756/1‑250 (MQ=255)                                                                                                 gccgGAAAGCGTATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGGCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGTTTGCGTGGCTCTTTTTCTGAGATACGCTGCAAATCCt                                                                                                                                          <  2:68666/251‑1 (MQ=255)                                                                                                   cgGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTgg                                                                                                                                        <  1:464380/251‑1 (MQ=255)                                                                                                     gAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGcc                                                                                                                                      <  1:354287/251‑1 (MQ=255)                                                                                                     gAAAGCTGATGCGGGTAAACAGCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCAGCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGcc                                                                                                                                      <  2:464560/251‑1 (MQ=255)                                                                                                           tGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTTGTT‑CCGCCCCGATTC‑CCCCTAATCGCTTTCCTAATCTTGGAACCCCC‑GGATTTGCCC‑GGGTTTAGGCCTGTCATCCGGTCCTTGAAGATCATCGC‑CACTTCGGCCCCCCCCCGGTTGTGGCCGTCTTTTTCTGAGATAAGCTGCAAATCCTCGCCGTTaa                                                                                                                                 >  1:36673/1‑250 (MQ=255)                                                                                                            gATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATCCCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTaaa                                                                                                                                <  2:26603/250‑1 (MQ=255)                                                                                                                  ggTAAACATCCAGACGCGAAGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACGCCAGt                                                                                                                         <  2:254663/251‑1 (MQ=255)                                                                                                                   gTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACCGAGGGTACTTTT‑TTTGCCGGACCGATAC‑ACATTAATGGCTTCCAAAATCTGGTAACCCCC‑GGGTTAACCC‑GGGTTAAAGCTGGTCATCGGGTCCCCGAAGATCATTGA‑CACTTCGGCAACCACCCGGTTGCGGCGCTCTTTTTCTGAGATACGATGCAAATCCttgacgcttaacttccgtt                                                                                                                        >  1:269579/1‑233 (MQ=255)                                                                                                                     aaaCACCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGttt                                                                                                                       <  1:118084/250‑1 (MQ=255)                                                                                                                       aCATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑GCCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTc                                                                                                                    <  2:430699/251‑1 (MQ=255)                                                                                                                             aggcgctaTGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGTAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCAt                                                                                                             <  1:411000/244‑1 (MQ=255)                                                                                                                                cgcgATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTAc                                                                                                          <  1:98607/251‑1 (MQ=255)                                                                                                                                              gggAGTACCGACCTGATTTAGCAGGTCAATCTCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAAGCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGGTAAGGCTGGTCAGCGGTTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGTTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑TCCATTACTCGTTCCGGATaat                                                                                            <  2:312096/251‑1 (MQ=255)                                                                                                                                                gAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTTT‑GTTTCCCCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGGAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTCGTCATCGGGGCCTGGAAGATCATCGC‑CCCTTCGGCCCCCACCAGGGTGCGGCGCTCTTTTTCTTCGATACGGTGCAAAACCTGGCCGTTAAACTCCCGTTTTTCTTGCC‑TTTCGCGGCCCGGATaaac                                                                                           >  2:218662/1‑248 (MQ=255)                                                                                                                                                  aTACCGTCGTGACTCAGCAGGTCACTCGCGCGCTGACGGCGGGTACGTTT‑GTGCCCGCCCTGAGGC‑ACCTTAATCGCTTCCATAATCGGGAAACCCCC‑GGTTTAGCAC‑TGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACACCAGTTTTTCT‑GCCATTACGATGCCCGGATaatcaat                                                                                        <  2:118946/251‑1 (MQ=255)                                                                                                                                                  aTACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCCCCAGGGTGCGGCGCTCTTTTTCTGAGATACCCTGCAAATCCTGGCCGTTAAACTCCCGTTTTTTT‑GCCATTAAGCGcccccggtaaacaat                                                                                        >  2:340748/1‑240 (MQ=255)                                                                                                                                                    aCCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTTACGCCCTGATGC‑ACCTTTATCGCATCCCTAATCTTGGAACCCCC‑GGTTTTG‑ACAGGGTTAAGGCTGGGCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCCCCAGGTTGCGGGGCTCTTTTTCTTGGATACGCTGCAAATCCTGGCCGTTAAAATACCGTTTTTTT‑TCCATTTCGCGGCCCGGATactcaatcc                                                                                      >  2:156982/1‑250 (MQ=255)                                                                                                                                                    aCCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑CTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAAACCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGGCCTGGAAGATCATCGC‑CACTTCGGCACCCACCCGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGCTTTTCT‑GCCATTACGCGGCCCGGGTaatcaatc                                                                                       >  1:239909/1‑250 (MQ=255)                                                                                                                                                               tCAGCAGGTCAATCGCTCGCTGACGGCGGGTTCTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAAt                                                                            <  1:38837/250‑1 (MQ=255)                                                                                                                                                                cagcagGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCg                                                                          <  1:28365/251‑1 (MQ=255)                                                                                                                                                                   cagGTCAATAGCTCCCCGCCGGCGGGCACTTTT‑GTTGCCGCCCTGATGCCACCT‑AAAAGCTTCCCTAATCTGGGAACCCAC‑GGGTTCGCCC‑GGGTTTCGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACACACCCGGTTGCGGCGCTCTTTTTTTCAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTTT‑GCCATTACCCGGCCCGGGTAATCCATCAACCCCATAATCGca                                                                        >  2:443574/1‑249 (MQ=255)                                                                                                                                                                             gctcgctGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGAc                                                             <  1:465582/251‑1 (MQ=255)                                                                                                                                                                               ccgctGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCg                                                           <  2:116528/250‑1 (MQ=255)                                                                                                                                                                                      cggcggGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTAcc                                                    <  1:204200/251‑1 (MQ=255)                                                                                                                                                                                          gggTACTTTT‑GTTGCCGCCCTGATGC‑ACATTAATCGATTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGACATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCgg                                                  >  2:79797/1‑249 (MQ=255)                                                                                                                                                                                                 ttt‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGAc                                          <  1:444678/250‑1 (MQ=255)                                                                                                                                                                                                  tt‑gttgCCGCCCTGATGC‑ACCTTAATCGCGTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACt                                         <  1:20919/250‑1 (MQ=255)                                                                                                                                                                                                     gttgCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCAc                                      <  1:16927/251‑1 (MQ=255)                                                                                                                                                                                                         ccgccCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCcac                                  >  2:113570/1‑251 (MQ=255)                                                                                                                                                                                                          cgccCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCcaca                                 <  1:442460/251‑1 (MQ=255)                                                                                                                                                                                                                gATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGGCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATcc                             <  1:156982/249‑1 (MQ=255)                                                                                                                                                                                                                  tGC‑ACCTTCATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATACTCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGAc                         >  2:208908/1‑251 (MQ=255)                                                                                                                                                                                                                          tAATCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATaa                                                                                             <  1:55206/176‑1 (MQ=255)                                                                                                                                                                                                                          tAATCGCTTCCATAATCTGGAAACACAC‑GGTTTAGCAC‑GGGTTACGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATaa                                                                                             >  2:55206/1‑176 (MQ=255)                                                                                                                                                                                                                           aaTCGCTTCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCCCTTCGc                 >  1:136059/1‑251 (MQ=255)                                                                                                                                                                                                                                  tCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGttt          >  2:85632/1‑251 (MQ=255)                                                                                                                                                                                                                                  tCCATAATCTGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGt            <  1:340748/249‑1 (MQ=255)                                                                                                                                                                                                                                        atcgggAAATCCAC‑GGTTTAGGAC‑GGGTTAAGGCGGGTCATCGGGTCTTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAAGCCTGGCCGTTAAACTCCAGTATTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGTCTTACCGGAGCCGGACTCACCCACAATCCCGTCCTCTTGGCCCTGTTTTACGCt    <  2:54411/247‑1 (MQ=255)                                                                                                                                                                                                                                           tGGAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGACCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGCGATACGCTGCAAATCCTGGCCGTTCAACTCTAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGACCTGACCAACTTACCGGCGCCGGACTCACCCCCAATCCCGACCACTTCGCCCAGTTTTACGCTGt  >  2:194953/1‑250 (MQ=255)                                                                                                                                                                                                                                            ggAAACCCAC‑GGTTTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGATCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACAGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGt  >  1:204347/1‑249 (MQ=255)                                                                                                                                                                                                                                            ggAAACCAGC‑GGTTTAGCAC‑GTGTTAAGGCGTGTCATCGGTTCCTGGAATAACATCGC‑CACTGCGGCTCCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGt  <  2:313931/249‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               AATGGTCACGTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTT‑GTTGCCGCCCTGATGC‑ACCTTAATCGCTTCCATAATCTGGAAACCCAC‑GGTGTAGCAC‑GGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGC‑CACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCT‑GCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGT  >  NC_000913/3703285‑3703766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑