Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,599,947	G→T	L14F (TTG→TTT)	gcvR →	putative transcriptional regulator GcvR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,599,947	0	G	T	100.0%	117.0 / NA	33	L14F (TTG→TTT)	gcvR	putative transcriptional regulator GcvR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/19);  total (14/19)

AGACATTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCACGACGGCGCGTCCGCGTCCGCCAATGCCAGCATC  >  NC_000913/2599703‑2600189                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   agacaTTACCTTTTTCATCCATCGGAGTACCAATCGCGACAATACTTCACGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATAACTGCGTTTGGTGcc                                                                                                                                                                                                                                              >  2:387419/1‑251 (MQ=255)  gacaTTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCg                                                                                                                                                                                                                                             >  1:9835/1‑251 (MQ=255)     aTTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATc                                                                                                                                                                                                                                          >  2:160063/1‑251 (MQ=255)              ttCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGa                                                                                                                                                                                                                                  <  2:277314/250‑1 (MQ=255)              ttCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGa                                                                                                                                                                                                                                  <  2:30810/250‑1 (MQ=255)                     atcGGAGTAACCATCGCGACAATACTTCACGTGAACATGGGCCCTCCTCTGTGCAAACAAGTATCTCCATGGTACGTTTGGTATGGCATTAAAAGCAAGCATACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCATTAAGAACAACTCCCCCCTTACAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGAAATGGTGac                                                                                                                                                                                                                          >  2:410443/1‑250 (MQ=255)                          aGTAACAATCGCGACACTACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGCCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACAcc                                                                                                                                                                                                                      >  2:407480/1‑250 (MQ=255)                               cAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCGGGAATTGTGAACACCATCAcc                                                                                                                                                                                                                <  1:388669/251‑1 (MQ=255)                                               tCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCagtagt                                                                                                                                                                                                 <  1:343587/250‑1 (MQ=255)                                               tCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCagtag                                                                                                                                                                                                  <  1:353557/249‑1 (MQ=255)                                                     gAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCt                                                                                                                                                                                          <  1:367300/251‑1 (MQ=255)                                                                      tgtgCACACAAGTGACTCACTGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGAAAGt                                                                                                                                                                         >  1:350821/1‑251 (MQ=255)                                                                             aCAAGTGTCTCAATGGAACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGACCACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTgg                                                                                                                                                                  >  2:128429/1‑251 (MQ=255)                                                                                                gTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGTTTTCCTTAAGAAAAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCCACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTACTATTGAAGACAGTCGCCTGGCGATGTTGGGAGAAGAGtt                                                                                                                                               >  1:162307/1‑251 (MQ=255)                                                                                                      tATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGtt                                                                                                                                         >  2:257454/1‑251 (MQ=255)                                                                                                                          gacagaACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGtt                                                                                                                     <  1:434045/251‑1 (MQ=255)                                                                                                                             agaACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCAt                                                                                                                  <  2:9835/251‑1 (MQ=255)                                                                                                                                               gtATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTg                                                                                                 <  2:212482/250‑1 (MQ=255)                                                                                                                                                 aTGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGATTGACACTGTCATCGCATCATTATCTGGTGTTCACTGCGGTTGGTGCCGATCGCCCTGGAATTGTGAACTCCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAATACTGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGAt                                                                                               <  1:235731/250‑1 (MQ=255)                                                                                                                                                          ttttttATGCGTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAAc                                                                                      <  1:387419/250‑1 (MQ=255)                                                                                                                                                                         ttAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCa                                                                                                                                             >  1:34284/1‑180 (MQ=255)                                                                                                                                                                         ttAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCAGCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCa                                                                                                                                             <  2:34284/180‑1 (MQ=255)                                                                                                                                                                                                          gtgtGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCTTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAgcgc                                     <  1:407480/248‑1 (MQ=255)                                                                                                                                                                                                           tttGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAgcgc                                     >  1:176129/1‑250 (MQ=255)                                                                                                                                                                                                              ggcacTGTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCacg                                  <  2:8851/248‑1 (MQ=255)                                                                                                                                                                                                                    gTCATCGCAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCACGACGgcgc                           >  1:127479/1‑251 (MQ=255)                                                                                                                                                                                                                      cATCGCAACATTATCTGGTGAGCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCACGACGGcgcg                          <  2:350821/250‑1 (MQ=255)                                                                                                                                                                                                                           cAACATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCACGACGGcgcgtccgcg                    <  1:461937/251‑1 (MQ=255)                                                                                                                                                                                                                            aaCATTATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGACTCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCACGACGGcgcgtccgcgt                   >  1:111401/1‑251 (MQ=255)                                                                                                                                                                                                                              cATTATCTGGTGATCACGGCGGTTGGTGCCGATCGCCCTGGAAGTGTGAACACCATCACCCGTCATGTCAGTAGTTGTGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGGGTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGGTGAAAGGTGCCGAACTGGAGCTTGGAATCGTGATGAAGCTCACGACGGCgcttccgcttcc                 <  2:162307/251‑1 (MQ=255)                                                                                                                                                                                                                                 tATCTGGTGATCACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGGAGTTGCTGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGTAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCACGACGGCGCgtccgcgtccgc               <  1:69678/250‑1 (MQ=255)                                                                                                                                                                                                                                            cACTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCACGACGGCGCGTCCGCGTCCGCCAATGCCAGCAt   >  2:416790/1‑251 (MQ=255)                                                                                                                                                                                                                                             ccTGCGTTTGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCAGGTCAGTAGTTGCGGCTGTAATATTTAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTGCACGTTTATCATGCTGCTTGCCTGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGGTGAAGCGCACGACGGCGTGTCGGCGTCCGCCAATGCCAGCAtc  <  2:277592/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   AGACATTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCGTGAACATGGGCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCATTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTGTATGCATGTTTTTTTTATGCTTTCCTTAAGAACAACTCACCCCTTAAAGGAATAACCAGTTTGACACTGTCATCGCAACATTATCTGGTGATCACTGCGTTGGGTGCCGATCGCCCTGGAATTGTGAACACCATCACCCGTCATGTCAGTAGTTGCGGCTGTAATATTGAAGACAGTCGCCTGGCGATGCTGGGAGAAGAGTTCACGTTTATTATGCTGCTTTCCGGTTCATGGAATGCCATTACTCTGATTGAATCAACGTTACCGTTGAAAGGTGCCGAACTGGATCTTTTAATCGTGATGAAGCGCACGACGGCGCGTCCGCGTCCGCCAATGCCAGCATC  >  NC_000913/2599703‑2600189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑