| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,358,859 | T→C | Y110C (TAT→TGT) | puuP ← | putrescine:H(+) symporter PuuP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,358,859 | 0 | T | C | 92.3% | 23.5 / NA | 13 | Y110C (TAT→TGT) | puuP | putrescine:H(+) symporter PuuP |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (5/7); minor base A (1/0); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCA‑GCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGG > NC_000913/1358624‑1359045 | aaCGCCGTCTCCTTTATGCAGTCCCTGAACTACCAGGAATATAAACTCCACCAGGATGGAGAGTTGCACCAGTACAAACAGGGGATTGAAGTTAGCGCCCAGGTTTACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTGACCACCCACACCCACGGCGGCACTTCCGTGAAGAGGTCGGAGAGATAGATTTTCGCCAACAATACGTTAATCATCGGCAAAAATAGACAATCCA‑GCAGCGAtt < 2:174243/251‑2 (MQ=255) ccTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCA‑GCAGCGATGACCAGCCg < 1:336872/249‑1 (MQ=255) gAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCGCACCCACGGGGGCACTTCCGGGAAGAGGGCGGAGAGGAAGATTTTCGCCAACAAAACGTTAATCATCGGCCAAAAGAGACAAACCA‑GCAGCGATGACCAGCCGCCCATAAATCCGACGTGc > 1:163465/1‑251 (MQ=255) aaCTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATTGCGACGGAGGTTACCACCCACACCCACGGCGGCCCTTCCGGGGAGAGGGGGGGGAGATAGAATTTCCCCAACAAGACGGTACTCATCGGCAAAAAGAGACACACCA‑GCAGCGATGAACAGCCGACCATAAATCCGACGTGCg > 2:148572/1‑251 (MQ=255) aaaCACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGTAAAAAGAGACAATCCA‑GCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCTACttt < 1:312713/250‑1 (MQ=255) ccaTGAAGAAGATTTGCACCAGTACAAACAGGGTATTGAATTTAGCGACCAGGTTGACGCTCTTCATATCCGCGTCGGGTAAAACGGCGACGAAGGTTACCACCCACACCCACGACGGCACGTCCGGGAAGAGGGCGGAGAGATAGATCTCCTCCACCAACACGTTAATCATCTGCAAGAAGAGACAATCCA‑ACAGCGACTACCAGCCGAACAGAAAGACGACGTTCGGATTAATCTTCTTTTgctcggag < 2:224575/251‑7 (MQ=255) tgatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGGTAAAAAGGCGACGAAGGGTTCCACCCAAACCCACGGCGGCACATCCGGGGAGAGGGGGGGGAGATCGATTTTCACCCACCAGACGGTAATCATCGGAAAAAAAAGAAAAGCCA‑GAAGAGGTGACCAGCCACCCCTAAATCCCAcgggcggggagaacgcctttttgccgtgaga > 2:317745/1‑227 (MQ=255) gAAGTTAGCGACCAGGTTGACGCCCGTCAGTTTCGCGGCGGGTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCA‑GCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAAcc < 2:178966/250‑1 (MQ=255) cGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAGTGCGACGAAGGGTACCACCCACACCCACGGCGGCCCTTCCGGGAAGGGGGGGGGGAGGTAGAATTTTGCCAACAAGACGGTAATCATCGGCCAAAAAAGACAATACACGC‑GAGATGAACAGCCGACCATAAAACCGACGTGCGGGGTAATCGGCTTTTTCGCGGAGGGATAGGACGGACCGGCCTCCCGAAACTGGCGAACCAGttttccc > 2:123705/1‑247 (MQ=255) gccGCTCTTCAGATTCCCGGCGGTTAACATGGCGACGAAGGTTACCTCCCACACCCACGGCGGCATTTCCGGGAATAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCA‑GCAGCGATGCCGAGCCGACCATAAACCCGACGTGCGGGTTAATCGGCTGGTGCGCGTAGGTAGAGGCCGAACCGGCCTCCGGAAACTGGGGAACCAGGTTGTCGTAGCTGATAg < 2:96867/249‑1 (MQ=255) cccacccacCCCCACGGCGGCACTCCCGGAAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATAACCGGCAAAAAGAGACAACCCA‑GCAGCGATGACCAGCCGACCATAAATCCGCCGTGCGGGTTAATCGCCTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTGGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCgg < 1:289086/250‑1 (MQ=255) ttacGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCA‑GCAGCGATGACCAGCCGACCAAAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCACATAGGCCGCCGGAACGTGACCGTCGCTAATGCCgg > 1:275909/4‑251 (MQ=255) cGGGAAGGGAGCGGAGAGACAGATTTTCGACAACAAGACGTTAATCATCGGCACAAAGAGACAATCCA‑GCAGCGATGACAAGCAGACCATAAATCCGCCGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGACGAACCGGCCTCCGGAAACTGGCGAACCCGTTTGCCGTCGCTGATAGCGGAAAACAGCACACCCGCCACCGCCAGCAAATCGGAAGCCGGAACGTGACCGTCGCTAATGcagg > 2:422146/1‑245 (MQ=255) | AACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCA‑GCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGG > NC_000913/1358624‑1359045 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |