Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,425,688	C→A	H57N (CAC→AAC)	priB →	primosomal replication protein N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,425,688	0	C	A	100.0%	201.0 / NA	58	H57N (CAC→AAC)	priB	primosomal replication protein N
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/36);  total (22/36)

CCGTGAGCGTCGCGATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACACGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTAC  >  NC_000913/4425444‑4425934                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       ccGTGAGCGTCGCGATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGaa                                                                                                                                                                                                                                                   >  1:6857/1‑250 (MQ=255)          tCGCGATGATTTCGCAACCGAAACCGCTGATGTTGCTGAAGCTGGGGATTTTGAAGAGTAATTTCTTATGACCAACCGTCTTGTTTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTATATGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTATCGGAAACGAAAACCaagt                                                                                                                                                                                                                                           <  2:350296/249‑4 (MQ=255)           cgcgATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGTCCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCAt                                                                                                                                                                                                                                        <  2:135618/251‑1 (MQ=255)            gcgATGATTTCGCAAACGCAACCGATGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAACACAGGCCAtt                                                                                                                                                                                                                                       >  2:398473/1‑251 (MQ=255)             cgATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGccctt                                                                                                                                                                                                                                       >  2:326762/1‑247 (MQ=255)                     tCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGAGGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAAGTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGGGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAg                                                                                                                                                                                                                               <  1:22186/250‑1 (MQ=255)                       gCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTACTTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTCTGCAGGGCTCCCCTTCGAAAGGTCAGTCCCTCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTTTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGtat                                                                                                                                                                                                                            >  1:297786/1‑251 (MQ=255)                        aaaaCGGAACCGATGAAGATGCTGAAGAGGGGGATTCTGAAGAGGAATTTCTGGTAACCAACCGGCTGGTATAGGCCGGCAGCGTGTGCAGGGCTCCCCTTCGAAAGGTTAGTCCAATAGGAATTCCTCACTACCAGTTCGTACTTGGGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGTGTGGAGTCAAATGCCCGTTATTGTTAGCGGAAGCGAAAAACATGGCGTTTCTCACAGtata                                                                                                                                                                                                                           <  2:222134/250‑1 (MQ=255)                          aaCGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAAc                                                                                                                                                                                                                         <  2:139078/251‑1 (MQ=255)                           aCGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTGTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACg                                                                                                                                                                                                                        <  1:279283/251‑1 (MQ=255)                            cGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACgg                                                                                                                                                                                                                       <  2:232447/251‑1 (MQ=255)                                          atgCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATa                                                                                                                                                                                                         <  1:89377/251‑1 (MQ=255)                                            gCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAAc                                                                                                                                                                                                       <  2:14443/251‑1 (MQ=255)                                               gAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCg                                                                                                                                                                                                     <  1:104533/250‑1 (MQ=255)                                                  gCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCCTAACCGTTc                                                                                                                                                                                                  >  1:274583/1‑250 (MQ=255)                                                     ggggATTCTGAAGAGTAATTTCTGAGGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGTGCTCCCCTTCGAAAGGGCAGTCCATCAGGAAGTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAgg                                                                                                                                                                                               <  1:301286/250‑1 (MQ=255)                                                         aTTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGttc                                                                                                                                                                                          >  2:386045/1‑251 (MQ=255)                                                              gAAGAGTAATTGCTGATGACCAACCGGCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCGTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAGCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGttcatttc                                                                                                                                                                                     <  2:119009/251‑1 (MQ=255)                                                                     aaTTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATCACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCcac                                                                                                                                                                              >  1:443633/1‑251 (MQ=255)                                                                            gatgaCCAACCGTCTGGGGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCa                                                                                                                                                                        >  2:78240/1‑250 (MQ=255)                                                                             atgaCCAACCGACTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCaa                                                                                                                                                                       >  2:186243/1‑250 (MQ=255)                                                                              tgaCCAACCGTCTGGTGTTGTCCGGCACCTTGTGCAGGGCTCCCCTTCGAACGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAATGCaa                                                                                                                                                                       >  2:346255/1‑249 (MQ=255)                                                                                       gTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCCTCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTAATCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAGAGAACGGACTg                                                                                                                                                            >  1:227357/1‑251 (MQ=255)                                                                                        tCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACACGGCAAAGAACGGACTg                                                                                                                                                            >  2:377772/1‑250 (MQ=255)                                                                                                    cGGCCCCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGttt                                                                                                                                               <  2:10953/251‑1 (MQ=255)                                                                                                    cGGCACCGTGGTCAGGGCTCCCCTTCGAAAGGTCAGTCAAGCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCAGTACTCACAGTAGAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGttt                                                                                                                                               <  2:39771/251‑1 (MQ=255)                                                                                                     ggCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGtttt                                                                                                                                              <  1:398473/251‑1 (MQ=255)                                                                                                        aCCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGc                                                                                                                                            >  1:44649/1‑250 (MQ=255)                                                                                                               gCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTGCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAg                                                                                                                                    <  2:131731/251‑1 (MQ=255)                                                                                                                cAGGGCTCCCCTGCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGc                                                                                                                                   <  1:403707/251‑1 (MQ=255)                                                                                                                    gCTCCCCTTCGAAAGGTCAGTCCATCAGGAATCCCGCACTGACAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATATCCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGat                                                                                                                               <  2:375511/251‑1 (MQ=255)                                                                                                                       ccccTTGGAAAGGTCAGCCCATCAGGAATTCCTCCCTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAAGGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTACAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGatt                                                                                                                              <  2:257963/249‑1 (MQ=255)                                                                                                                       ccccTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTTAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGatt                                                                                                                              <  2:93142/249‑1 (MQ=255)                                                                                                                            tCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCATTTCGTGCTTGAGCATCGTGCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGGTCAGGGTTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGattgaatt                                                                                                                         <  2:286663/249‑1 (MQ=255)                                                                                                                            tCGAAAGGTCAGTCCATCAGGAACTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGATCGGACTGAGCAAAATGGTTTTGCATGCCGATCAGagtgaattg                                                                                                                        <  1:94937/250‑1 (MQ=255)                                                                                                                            tCGAAAGGGCATTCCATTGGGGATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGAAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTAAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGagtgaattg                                                                                                                        <  2:172134/250‑1 (MQ=255)                                                                                                                                aaGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGTCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAAGTGATAGa                                                                                                                   <  2:401481/251‑1 (MQ=255)                                                                                                                                       gTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGga                                                                                                            <  1:430768/251‑1 (MQ=255)                                                                                                                                           aTCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGTAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATGGATAGATTCTGGAGACt                                                                                                        <  2:274583/251‑1 (MQ=255)                                                                                                                                               ggAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCAGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAACAGATTGAATTGATAGATTCTGGAGACTAGcc                                                                                                    >  1:272286/1‑251 (MQ=255)                                                                                                                                                gAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCGGGAGACTAGCCa                                                                                                   <  1:377772/251‑1 (MQ=255)                                                                                                                                                   ttCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGGTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAACCatat                                                                                                >  2:245020/1‑251 (MQ=255)                                                                                                                                                     ccTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATgg                                                                                              >  2:89290/1‑251 (MQ=255)                                                                                                                                                                                    cTGTGCAGGAGGCAGCCGGCTTTCACCGGCAGGCGTGGTGTCACATGCCCGTTATTGTTAGCGGAAACGCAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCCAGTGCTGCCg                                                               >  2:52233/1‑251 (MQ=255)                                                                                                                                                                                                   ccGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACCGTATAACGGACGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCCAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGCAgg                                                >  2:52893/1‑251 (MQ=255)                                                                                                                                                                                                         ttCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTc                                           <  1:159126/250‑1 (MQ=255)                                                                                                                                                                                                                 cAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAag                                        >  1:68605/1‑245 (MQ=255)                                                                                                                                                                                                                      gtggtgTCAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGGCGGCAGTCGCATAACCGTGCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATGGAATTGATAGACTCGGGAGACTAGCCATATGGCACGTTATTTCCGGCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTGCAAGAGATCGACtat                             <  2:428690/251‑1 (MQ=255)                                                                                                                                                                                                                      gtggtgTCAAATGCCCGTTATGGTTAGCGGAAACGAAAAACAGGCCATTACGCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACtat                             <  1:304211/251‑1 (MQ=255)                                                                                                                                                                                                                             cAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGat                       <  1:380087/250‑1 (MQ=255)                                                                                                                                                                                                                             cAAATGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGACGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGAATAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGat                       >  1:228343/1‑250 (MQ=255)                                                                                                                                                                                                                               aaTGCCCGTTATTGTTAGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATc                    <  1:89290/251‑1 (MQ=255)                                                                                                                                                                                                                                      gTTATTGTTAGCGGCAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGc             >  1:136444/1‑251 (MQ=255)                                                                                                                                                                                                                                         aTTGTTAGCGGAAACGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTg           <  1:5491/250‑1 (MQ=255)                                                                                                                                                                                                                                            gTTAGCGTAAACGAAAAACAGGCCATTACTCCCAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGACACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGATCAGATTGAATTGTTAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGaaa        <  2:469394/250‑1 (MQ=255)                                                                                                                                                                                                                                               aGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAAc     <  1:63708/250‑1 (MQ=255)                                                                                                                                                                                                                                               aGCGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACt    <  1:78240/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cGGAAACGAAAAACAGGCCATTACTCACAGTATAACGGTCGGCAGTAGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTAc  >  2:325264/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       CCGTGAGCGTCGCGATGATTTCGCAAACGAAACCGCTGATGATGCTGAAGCTGGGGATTCTGAAGAGTAATTTCTGATGACCAACCGTCTGGTGTTGTCCGGCACCGTGTGCAGGGCTCCCCTTCGAAAGGTCAGTCCATCAGGAATTCCTCACTGCCAGTTCGTGCTTGAGCATCGTTCTGTGCAGGAGGAAGCCGGCTTTCACCGGCAGGCGTGGTGTCAAATGCCCGTTATTGTTAGCGGACACGAAAACCAGGCCATTACTCACAGTATAACGGTCGGCAGTCGCATAACCGTTCAGGGGTTCATTTCATGCCACAAGGCAAAGAACGGACTGAGCAAAATGGTTTTGCATGCCGAGCAGATTGAATTGATAGATTCTGGAGACTAGCCATATGGCACGTTATTTCCGTCGTCGCAAGTTCTGCCGTTTCACCGCGGAAGGCGTTCAAGAGATCGACTATAAAGATATCGCTACGCTGAAAAACTAC  >  NC_000913/4425444‑4425934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑