Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,696,157	G→T	P10H (CCT→CAT)	yhjR ←	PF10945 family protein YhjR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,696,157	0	G	T	100.0%	220.9 / NA	58	P10H (CCT→CAT)	yhjR	PF10945 family protein YhjR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/37);  total (21/37)

AATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCACATGCTAAAT  >  NC_000913/3695921‑3696380                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                aaTGCGGCGGTGATGGTTGTTGCCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGt                                                                                                                                                                                                                   <  2:326464/251‑1 (MQ=255)             aTGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGGATCTGGTTCATTGTTATTc                                                                                                                                                                                                       <  1:409424/251‑1 (MQ=255)                          ccACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATc                                                                                                                                                                                          >  1:50740/1‑251 (MQ=255)                              gCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCCGCCCGCAACGGACAGCGTTTTAATGCCGCGGCAAACTGTTCGCGTTGGGAAATATCGGCACACTCAATATCAGGCAGTGAAACTGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGACTATCGCGGGCACATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCt                                                                                                                                                                                       >  1:457178/1‑250 (MQ=255)                                                 tATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTc                                                                                                                                                                   >  1:48618/1‑251 (MQ=255)                                                 tATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACGGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGtt                                                                                                                                                                    <  2:425170/250‑1 (MQ=255)                                                  aTCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCa                                                                                                                                                                  <  1:273860/251‑1 (MQ=255)                                                    cccAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTATGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACACTATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGGACCGTTCATg                                                                                                                                                                >  2:414290/1‑251 (MQ=255)                                                          aCGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTGCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCGTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGCTCATGAGACaa                                                                                                                                                          <  2:380040/251‑1 (MQ=255)                                                            ggCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAAtt                                                                                                                                                        >  1:156729/1‑251 (MQ=255)                                                                    aCCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGt                                                                                                                                                >  1:314876/1‑251 (MQ=255)                                                                           cccTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTGCGCGTTGTGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTTTTATTCATTTTGCTTGATCCCTAGGAAATAACCGTACCGTTCATGAGACAATTCTAACCTTGAGCAtt                                                                                                                                         <  2:48618/251‑1 (MQ=255)                                                                                    tgttgCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGt                                                                                                                                 <  2:6818/250‑1 (MQ=255)                                                                                        gcgcAACCTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTAt                                                                                                                             <  2:74023/250‑1 (MQ=255)                                                                                        gcgcAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTAtt                                                                                                                            <  2:116348/251‑1 (MQ=255)                                                                                              aCTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGGTTATTGTTTAc                                                                                                                      <  2:173426/251‑1 (MQ=255)                                                                                                   gCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCg                                                                                                                  <  2:123309/250‑1 (MQ=255)                                                                                                   gCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGGTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTGATTCATTTGGCTTGATCCCGATGAAATAACCGTACCTTTCATGAGACAATTCTGACCGTGAGCATTTCGGCTGTTGATTGTTTACGGc                                                                                                                   <  2:50574/249‑1 (MQ=255)                                                                                                    ccAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCgag                                                                                                                <  1:66520/251‑1 (MQ=255)                                                                                                      aGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGAc                                                                                                              <  1:419800/251‑1 (MQ=255)                                                                                                       gCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACg                                                                                                             <  1:315051/251‑1 (MQ=255)                                                                                                            ggCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATCTAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAAt                                                                                                        >  1:317124/1‑251 (MQ=255)                                                                                                                aGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGc                                                                                                    <  1:129273/251‑1 (MQ=255)                                                                                                                           gCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACGTTTTGa                                                                                         <  2:62380/251‑1 (MQ=255)                                                                                                                              gcgGCCAACTGTTCGCGTTGGGAAATATCGGCATAAGCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACtt                                                                                      <  2:8177/251‑1 (MQ=255)                                                                                                                               cgGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACttt                                                                                     <  1:177264/251‑1 (MQ=255)                                                                                                                                ggCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTc                                                                                    <  2:406640/251‑1 (MQ=255)                                                                                                                                 gCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTcc                                                                                   >  2:65600/1‑251 (MQ=255)                                                                                                                                   cAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGt                                                                                 >  2:445710/1‑251 (MQ=255)                                                                                                                                          tCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCa                                                                           <  1:326406/250‑1 (MQ=255)                                                                                                                                             cgTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGTAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAg                                                                       <  2:317124/251‑1 (MQ=255)                                                                                                                                              gTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAAc                                                                         >  1:389667/1‑248 (MQ=255)                                                                                                                                                 gggAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCa                                                                           >  2:35675/1‑243 (MQ=255)                                                                                                                                                 gggAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATGTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCa                                                                           <  1:35675/243‑1 (MQ=255)                                                                                                                                                          cGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTaaaa                                                           >  1:348643/1‑250 (MQ=255)                                                                                                                                                           ggCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTTACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAAc                                                         <  1:414290/251‑1 (MQ=255)                                                                                                                                                           ggCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAAc                                                         <  2:258864/251‑1 (MQ=255)                                                                                                                                                            gCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACg                                                        <  2:389667/251‑1 (MQ=255)                                                                                                                                                               tAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACgg                                                                                                                    >  2:79564/1‑188 (MQ=255)                                                                                                                                                               tAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACgg                                                                                                                    <  1:79564/188‑1 (MQ=255)                                                                                                                                                                   taaTATCAGGCAGGTAAAATTCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTTTCGCGGTATCATGCAGATTATCTGGTTCATTTTTATTCATTTTTCTTGATCCCTATGGAATAACCGTTCCTTTCAGGAGACAATGCTAACCGTGATCATTTCGGCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTGACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGa                                                  <  1:272575/249‑1 (MQ=255)                                                                                                                                                                      tatCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTa                                               >  1:245483/1‑250 (MQ=255)                                                                                                                                                                          aGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATc                                          >  2:134885/1‑251 (MQ=255)                                                                                                                                                                              aGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCacac                                      <  2:351096/251‑1 (MQ=255)                                                                                                                                                                               gTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACg                                     >  1:268018/1‑251 (MQ=255)                                                                                                                                                                                   aaaTGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCtt                                 <  1:183541/251‑1 (MQ=255)                                                                                                                                                                                    aaTGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTAAAGCGGGATCATGCAGAGTATCTGGATCCTTGTTATTCATTTTGATTGATCCCTCTGCAATAaccgtacc                                                                                                                                                                       >  1:286504/1‑116 (MQ=255)                                                                                                                                                                                    aaTGCCTGCTCTAACGCCACAATATCATTCTGGAAGATATGGTCTATCGCTGGATCATGCAGAGTAGCTGGTTCATTGTTATTCATTTTGCTTGATCCCTTTGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCTTTGCGCCGTTTTTTTGTTTACGGCTAGACGGGAATTTGCTCACTTTTTGACTTGCCGTTACGCAACAGAAAAAGGTAATACCGAAAAGACCAAAATCACACGTCTtg                                <  2:450953/251‑1 (MQ=255)                                                                                                                                                                                       gCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAATTTAAAAACGAAAAGACTAAAATCACACGTCTtgtg                              <  1:134885/250‑1 (MQ=255)                                                                                                                                                                                           gCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCt                          <  1:21152/250‑1 (MQ=255)                                                                                                                                                                                                 aCGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGc                                                                                                                                     <  1:365285/137‑1 (MQ=255)                                                                                                                                                                                                 aCGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGc                                                                                                                                     >  2:365285/1‑137 (MQ=255)                                                                                                                                                                                                           atCATTCTGGAAGATATAGCCTATCGCGGGCTCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCACAt         >  1:40719/1‑251 (MQ=255)                                                                                                                                                                                                                   ggtaGATATAGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCGTGTGACCTTCTAAATTGATTCACATGCTAAAt  <  2:153542/247‑1 (MQ=255)                                                                                                                                                                                                                          ataGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACg                                                                                                             >  1:249903/1‑136 (MQ=255)                                                                                                                                                                                                                          ataGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACg                                                                                                             <  2:249903/136‑1 (MQ=255)                                                                                                                                                                                                                          ataGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACtt                                                                                      >  2:150949/1‑159 (MQ=255)                                                                                                                                                                                                                          ataGCCTATCGCGGGATCATGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCAGTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACtt                                                                                      <  1:150949/159‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                AATGCGGCGGTGATGGTTGTTGTCCCCACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAAACGAAAAGACTAAAATCACACGTCTTGTGACCTTCTAAATTGATTCACATGCTAAAT  >  NC_000913/3695921‑3696380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑