Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,042,171	G→A	intergenic (+146/‑197)	zinT → / → yodB	metal‑binding protein ZinT/putative cytochrome b561 YodB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,042,171	0	G	A	100.0%	34.0 / NA	11	intergenic (+146/‑197)	zinT/yodB	metal‑binding protein ZinT/putative cytochrome b561 YodB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/4);  total (7/4)

GGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTGAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTACATTGGATAACGCT  >  NC_000913/2041953‑2042414                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    ggAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATAc                                                                                                                                                                                                                     >  1:105023/1‑251 (MQ=255)                     atCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATAcc                                                                                                                                                                                                 <  2:172977/251‑1 (MQ=255)                        cATATCAGTTGAGAAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGAATTCTCATGATAACGCCTCGCTGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATACGCTAACCCGCATTAAGTTAACCAATAACGGATTCAATACACAATACGGCCAACAGCGAAAGATAACtgg                                                                                                                                                                                              >  2:449094/1‑250 (MQ=255)                               gTTGAGTAGCGTAGAAGTGGTCGAGGAAATGATGTCGCATTTAGTTTTCTCAGGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACGGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACg                                                                                                                                                                                       <  2:316898/251‑1 (MQ=255)                                                                        gAGTATTCTCATGATAACGCCTCAATGCCGCTTTAGTAAGTTACCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACACTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAAt                                                                                                                                               <  2:181120/250‑1 (MQ=255)                                                                                                                 tATCATAACTGCCCCTGGTCCATACACCAACGCCACTGAACGCAAGCTAGCTACAGACACGCTACTCACTATGACGAGTCTGTAACTTAATAAGCTAACCAGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTCTCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATCAGGa                                                                                                      >  2:459446/1‑250 (MQ=255)                                                                                                                                                            aaGCTAGCTCCAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAgg                                                           >  1:137656/1‑250 (MQ=255)                                                                                                                                                                         acacGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATg                                              >  2:450144/1‑250 (MQ=255)                                                                                                                                                                               tcatcaCTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGAt                                       >  2:326967/1‑251 (MQ=255)                                                                                                                                                                                                               cTAACCCGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTACATTGGAt        >  1:57313/1‑250 (MQ=255)                                                                                                                                                                                                                    ccGCATTAAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTACATTGGATAACgct  <  1:147377/251‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    GGAAAACTGGCCAACGTATTATCCATATCAGTTGAGTAGCGAAGAAGTGGTCGAGGAAATGATGTCTCATTGAGTATTCTCATGATAACGCCTCGATGCCGCTTTAGTAAGTTATCATAACTGCCACTGGTCCATCCACAAACGCCACTGAACGCAAGCTAGCTACAGACACGCTCATCACTATGACGTGTCTGTATATTAATAAGCTAACCCGCATTGAGTTAACCAATAACGGATTCCATACACAATACGGCCAACAGAGAAAGATACCTGTGCTCACGCCATTGCTTATATTGGCTGGTTACAATATGCACTATCAATTTTTTAAATAACAAATTATTAAAACCACATCTGTATAAGGAATTTTTAAGGTTCGTGGGATAGCTTGACTGTGAAAATCACAGGAGCTACAAAAATGAACCGATTCTCAAAAACTCAAATTTATTTACATTGGATAACGCT  >  NC_000913/2041953‑2042414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑