| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,287,040 | G→A | intergenic (+56/+26) | ychS → / ← rttR | putative uncharacterized protein YchS/small RNA RttR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,287,040 | 0 | G | A | 94.1% | 38.9 / NA | 17 | intergenic (+56/+26) | ychS/rttR | putative uncharacterized protein YchS/small RNA RttR |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (10/6); minor base C (1/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATG‑GGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAG > NC_000913/1286827‑1287275 | ccgtttGGGCAGAATATTTGATTGGGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATG‑GGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg < 1:321288/247‑1 (MQ=255) gAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTTGCCCTTAGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATG‑GGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGGGGGGGAAGGATTACTCAGCGCTgcgcg > 2:272110/1‑220 (MQ=255) gAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATG‑GGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg < 1:272110/220‑1 (MQ=255) ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATG‑GGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTACGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGt > 1:75398/1‑251 (MQ=255) ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATG‑GGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGAATTTTGAACGTGATGGTGGTGGGGGAAGGATTACTCAGAGCGGCGCGCTTCGCCCTTCGGGTCGATGCCTGAGGCAACGCTCTCTCGCTGACGCTCCAGTCgg > 1:247028/1‑250 (MQ=14) ccgcTGCGCCCGTCGTCCTTCGGTTCGGTGCCTGCGGCAACGCTCTCCCGCTGACGCTCGAGTCGATCCTTAGTCGAAGCCTGTCATCCTTGACCGC‑TGCGGCAGAACATTTGATTGCGGATTCGCGAAATAGTTCGGGTACTTTGGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTgt < 2:24987/250‑2 (MQ=14) gTTGCATGCGGCAACGCACTCTCGCTGACGCTCGCGCCGAACATTAGTCGAAGCTTCTCATCCTTCCCCGCATG‑GGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGTTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCATTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTGCGCTCGCGTCGAACCTTGGTAGAAGCTTCTCATACTTCCCCGCAt > 2:443754/1‑251 (MQ=17) ctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTGCCCCGCATG‑GGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGGGAGGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAt < 1:443754/249‑1 (MQ=255) gAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATG‑GGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa > 1:83637/1‑250 (MQ=255) cGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATG‑GGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTc < 1:144944/174‑1 (MQ=255) cGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATG‑GGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTc > 2:144944/1‑174 (MQ=255) gAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATG‑GGCAGAATATTTGATTGCGGATTCGCTTAAGCGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTACGCCCTTCGCCCTTGGTGTCGTTGCTTGCGGCAACGCTCTCTCGCTGGCGCTCGAGGGGAACCTTGGTCGTAGCTTCTCTTCCTTCCCCGCGTGGGGAGGATATTTACTTgtcgattggtgcggttcttcc > 2:188814/1‑231 (MQ=14) gAAGCTTCTCATCCTTCCCCGCATG‑CGCAGAATCTTTGATAGCGGATTCGCTTCAGCGTCCAGGGCCTTTTGAAAGTGATGGTGGTGGGGGAAGGCTTACTCAGCGCTGCGCGCTTCGCACTTCGGGTCGtt > 2:232953/1‑132 (MQ=14) aTG‑GGCAGAATATTTGATTGCGGCTTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCGGGCGCTCGCGGCGGACCTTGGTCGACGCTTCTCATCCTTCCCCGCCTGGGCAGAATATTTCAGTGCGGAATCGTTGGGTAGTTCAGGGCATTTTGTACGTGATGGTGGGGGGGGaggg > 2:123513/1‑248 (MQ=14) gATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGCTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGa > 2:467356/1‑250 (MQ=255) gATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGATTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAACTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTATTGGCGGATTCGTTGGGAAGTTCAGGGACTTTTGACAGTGATGGTGGTGGGGGAGGGATTCGCACCTTCGa > 2:341508/1‑250 (MQ=17) attggtgATTCGCATAAGATTTCAGGTACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAg < 1:306592/245‑1 (MQ=17) | CCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATG‑GGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAG > NC_000913/1286827‑1287275 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |