Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,829,887	G→A	A293A (GCC→GCT)	astA ←	arginine N‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,887	0	G	A	100.0%	108.9 / NA	34	A293A (GCC→GCT)	astA	arginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/20);  total (14/20)

CCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGAT  >  NC_000913/1829656‑1830132                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      ccTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGGCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGTGCATCCAGTTGTGCGGCGGTTAAAAGCAAACGCGCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCATTCAGGCTGGGa                                                                                                                                                                                                                                    <  2:311050/251‑1 (MQ=255)            aCCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTGCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTg                                                                                                                                                                                                                          <  1:48964/250‑1 (MQ=255)                     cGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGc                                                                                                                                                                                                                <  1:55999/251‑1 (MQ=255)                         tCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCGCGGTTGCCGGATCGGTACGCACCAGCCCCTCGCGGAAATGGTGATAATTTGCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCt                                                                                                                                                                                                             <  1:58396/250‑1 (MQ=255)                                                           aCCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTATGACACCTCCACCAGCCGACTTTTACgggaggg                                                                                                                                                                          >  1:420784/1‑246 (MQ=255)                                                                    ccATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGGGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTTATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACGTTTACGGATGGCGCGCCcgcg                                                                                                                                                                 <  2:391087/251‑1 (MQ=255)                                                                             cATGCGGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGt                                                                                                                                                         <  1:255623/250‑1 (MQ=255)                                                                                 cTGTTTTCTCCTCTGCGCACAGGCGCACCAGCCGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAACTCACACGCTCGGCTGCCGGATCGGTACGCACCAGCACCACGCGGAAAAGGTGCTAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGAGCCGGCTGCCCTTCTGCCCCTTCCACCAGCCGACTTTTACGGATGgcgcgcccccggcgccagttacac                                                                                                                                                    >  1:188905/1‑233 (MQ=255)                                                                                       tctcCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGc                                                                                                                                              >  1:106298/1‑251 (MQ=255)                                                                                                                 cGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGat                                                                                                                    <  2:251392/251‑1 (MQ=255)                                                                                                                         aTCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTAAGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCCCTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTAc                                                                                                            >  1:91011/1‑251 (MQ=255)                                                                                                                                   tGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCCCTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTcc                                                                                                                               >  1:327832/1‑221 (MQ=255)                                                                                                                                   tGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCa                                                                                                                               <  2:327832/222‑1 (MQ=255)                                                                                                                                       aTTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTc                                                                                                                                           <  1:105064/206‑1 (MQ=255)                                                                                                                                        tttGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGGACGCACCAGCACCACCCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCCGGCCGCCCTTCTGCCACTTCCCCCCGCCGACTTTTACGGCTGGCGCCCACGCCGGCGAGGTCACACTCACGCGTCCGCCCCCCCGCAAAGATGTCGATATAGGTACGGTAACGAAAACctt                                                                                             >  2:435418/1‑251 (MQ=255)                                                                                                                                                 aTCCAGTTGTGCGGCGGTTTAAATCAAACGCTCGGTTGCCGGATCGGTACGAACCAGCACCACGCGGGAATGGGGATAATTTTCATTAGCGCCCAGGCAGGCCGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGCCTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTccc                                                                                    >  1:285396/1‑250 (MQ=255)                                                                                                                                                   ccAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGGCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAg                                                                                   <  1:205653/250‑1 (MQ=255)                                                                                                                                                     aGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCg                                                                                                                                                                                                                               <  1:447756/108‑1 (MQ=255)                                                                                                                                                     aGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGATGGGGAATCg                                                                                                                                                                                                                               >  2:447756/1‑108 (MQ=255)                                                                                                                                                         gggcggcgGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAAGTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACcgcg                                                                            <  1:233802/249‑1 (MQ=255)                                                                                                                                                                     aaaTCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAACGATGTCGATATAGTTACGGTAGCGAAAACATTCTTTCTCCAGCACAGCGCGGGCAGGcgcg                                                                >  1:388906/1‑251 (MQ=255)                                                                                                                                                                         cAAACGCTCGGTTGCCGGAGCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTGCTTGCTCCAGCACCGCGCGGGCAGGCGCGGtt                                                             <  2:387754/250‑1 (MQ=255)                                                                                                                                                                                ccGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCGGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCAGCGCGCGGTCAGTCGCGGTTGGCGGATg                                                     <  2:153862/250‑1 (MQ=255)                                                                                                                                                                                           aTCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGAACAGGCAGGCTGGGAAAACGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCAATATAGTTACGGTAGCGCAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCg                                           >  1:162453/1‑250 (MQ=255)                                                                                                                                                                                                 aCGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACg                                     <  1:435418/250‑1 (MQ=255)                                                                                                                                                                                                      ccagcaccaCGCGGAAATGGTGATAATTTTCATTAGCGCCCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGCATTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTgg                               <  2:383251/251‑1 (MQ=255)                                                                                                                                                                                                           accacGCGGAAATTGTGATAATTTTCATTAGCGACCAGGCAGGCGGGGAAAGCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGGCTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCGCCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGTATGTACCTGACCGATGACGTCCTGGGCTTc                          <  2:431142/251‑1 (MQ=255)                                                                                                                                                                                                            ccacGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTcc                         >  1:329850/1‑251 (MQ=255)                                                                                                                                                                                                             cacGCGGAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCt                        <  2:131572/251‑1 (MQ=255)                                                                                                                                                                                                                  ggAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGAt                   >  1:463691/1‑251 (MQ=255)                                                                                                                                                                                                                   gAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTc                          >  2:177801/1‑243 (MQ=255)                                                                                                                                                                                                                   gAAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTc                          <  1:177801/243‑1 (MQ=255)                                                                                                                                                                                                                             tAATTTTCATAAGCGACGCGGAAGGCTGGGCAATCGCGCTGAGCAGGCTGACCTTATGCCACTTCCACCCGCCGCCTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGCTGTCGATATAGTTAAGGTAGCGAAAACCTTCTTTCTCAAGCACCGCGAGGGCAGGCGAGGTTTGCGGATGTACCTGACCGATACCGCCCTGGGCTTACTGGGGTTAAAAGAgggg        >  2:313904/1‑250 (MQ=255)                                                                                                                                                                                                                                    cATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGAt  <  2:457056/250‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      CCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGGAAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGAT  >  NC_000913/1829656‑1830132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑