Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,414,829	A→G	E28G (GAA→GGA)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,414,829	0	A	G	100.0%	147.2 / NA	45	E28G (GAA→GGA)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/25);  total (20/25)

GTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCT  >  NC_000913/2414602‑2415075                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       gTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTTGCGTGATCCGTGCAATGGGACGCCAAGGCGTTCGGCTGAGCg                                                                                                                                                                                                                                 >  2:388787/1‑251 (MQ=255)                          cGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCCGCGTCGGGCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGCCGCAAAGGCGGTCGTCTGAGCGTTTTCAAACCTATCGCTCCGCcgcg                                                                                                                                                                                                        >  2:98999/1‑251 (MQ=255)                                   ggtgaTCGCGCAAGACGCGAGCCGCCTGACTGCCTGAGTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTgg                                                                                                                                                                                               <  2:368671/247‑1 (MQ=255)                                    gTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGc                                                                                                                                                                                              <  1:32268/251‑1 (MQ=255)                                             cAAGACGCGAGCCGCCTGACTGCCTGATTGCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGccc                                                                                                                                                                                     <  2:61031/251‑1 (MQ=255)                                                                  gCCTGATTTCACACCGTCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATcgt                                                                                                                                                                 <  1:383243/250‑1 (MQ=255)                                                                    cTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGGAACGAAAGGGGATAAACCGTGTCCCGGCTTATTATGCTGATCCCTACCGGAACCAGCGTCCGGCTGACCAACGTCCGCCTTGGCGTGATCCCTTCAATGGGACGCAAAGGCGTTTGTCTGAGCGGTTTTAAACCTCTCGCCTAGCCTCGTACCGGTGGCGCAGCGCCCGATCCGACTACGACAATcgtgcg                                                                                                                                                              >  2:321500/1‑251 (MQ=255)                                                                                   cagctcagctGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCa                                                                                                                                                                                <  1:301817/218‑1 (MQ=255)                                                                                      ctcagctGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGGAAGAGGATAAAACGGGGCCCGTATTATTATGATGAACCCTACCGGAACCAGCGTCGGTCAGAACAGCGGCAGCCTTGGCGTGAAACGTGCAAAGGGACGCAAAGGAGTTCGTCTGAGGGTTTTAAAACCTATCGCTCAGACGCGTAGCGGGGGCGATGCGCCCGATCCGGATACGACTATCGTGCGGGCGAACTCTTCccccac                                                                                                                                            >  1:223939/1‑251 (MQ=255)                                                                                         agctGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTccacca                                                                                                                                             >  1:100985/1‑247 (MQ=255)                                                                                         agctGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTccacca                                                                                                                                             <  2:100985/247‑1 (MQ=255)                                                                                                      gTTTTGTAACCCTCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCGGACCAGCGTCAGCGTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGGCTGATCGTGTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGccgctgaacc                                                                                                                             <  1:421585/250‑1 (MQ=255)                                                                                                       ttttGTAAACCACCAAATCCGCGCGAACGCAAGAGGGTAAACAGTGTCCCGTAGTATTATGCTGATCCCTACAGGAAAAAGCGTAGGTATGAACAGAGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCACAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGCCTCAGACTATCTGGCGGGCGAACTCTTCCACCACCCCGGccgctgaaccgc                                                                                                                           >  2:298349/1‑251 (MQ=255)                                                                                                        tttGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGCCGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGCTCAGACTACGACTATCGTGCGGGCGAACTCTTCCACCACGACGGccgctgaaccgct                                                                                                                          >  1:385768/1‑251 (MQ=255)                                                                                                            tAACCCGCCAAATCGGCGGTAACGAAAGAGGCTAAACCGTGTCCCGTGTTATTATTCTGATCCCTACCGGAACCAACGTCGGTCTGCACAACGTCAGCCTTTGCGTTATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGCGCGGTTTCAAAACTATCGCTCAACCGCGGAACCGTGGCGCTGCGCCCGATCAAACTACGATTATCGTGCGTGCGGACTCTTacaccaaccccgccgctgtaccgcagaa                                                                                                                       >  2:1456/1‑228 (MQ=255)                                                                                                             aaCCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCCAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGaaa                                                                                                                      >  1:443217/1‑250 (MQ=255)                                                                                                              aCCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAAt                                                                                                                    <  2:297002/251‑1 (MQ=255)                                                                                                                ccGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATg                                                                                                                   >  1:463521/1‑250 (MQ=255)                                                                                                                ccGCCAAATCGGCGGTAACCAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGGCTGACCAGCGTCATCCTTGGCGTGATCCGTTCAATGGGACGCAAAGGCGTTCGTCTGATCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACCACGACTATCGTGCGTGCGAACTCTTCCACCACGACTTCCGCTGAACCGCTGAAAATGa                                                                                                                  <  2:219768/251‑1 (MQ=255)                                                                                                                  gCCAAAGCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATGTTTATGCTGATCCCTACCGGAACCAGCGGCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATTGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACGCTTCCACCACGACGGCCGCTGAACCTCTGAAAATGAGc                                                                                                                <  2:406605/251‑1 (MQ=255)                                                                                                                     aaaTCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTAc                                                                                                             <  1:340758/251‑1 (MQ=255)                                                                                                                                aaCGAAAGAGGATTAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCt                                                                                                  <  1:394521/251‑1 (MQ=255)                                                                                                                                aaCGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGACCCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCcaccac                                                                                                                                            >  1:424377/1‑209 (MQ=255)                                                                                                                                aaCGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCGTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGATTATCGTGCGTGCGAACTTTTCgaccac                                                                                                                                            <  2:424377/209‑1 (MQ=255)                                                                                                                                       gagGATAAACCGTGTTCCGTATTATTATGCTGAGCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTcc                                                                                           <  2:466571/251‑1 (MQ=255)                                                                                                                                         ggATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCGTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCGGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAg                                                                                         <  2:443217/251‑1 (MQ=255)                                                                                                                                             aaaCCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTGTCCAGCAAt                                                                                     <  1:98999/251‑1 (MQ=255)                                                                                                                                                  gtgtCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGaa                                                                                <  1:5250/251‑1 (MQ=255)                                                                                                                                                    gtCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAg                                                                              <  1:101415/251‑1 (MQ=255)                                                                                                                                                          tattattatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGAACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCCCGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGACGGTCTGCTTTCCAGCAATCAGaa                                                                                >  1:469815/1‑243 (MQ=255)                                                                                                                                                          tattattatGCTGATCCCGACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTGCGTCTGAGCGTTTGCAAACCTATCGCTCAGCCGCGTACCGGTGTCGATGCGCCCGATCAGACTACGGCTATCGGGCGTGCGAACTCTTCCGCCACGACGTCCGCTGAACCGCTGAAAATGAGCGATGTTGAAGGTCTGCTTTCCAGCAATCAGaa                                                                                <  2:469815/243‑1 (MQ=255)                                                                                                                                                           attattatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCt                                                                       <  1:388787/251‑1 (MQ=255)                                                                                                                                                             tattatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTg                                                                      >  1:410476/1‑250 (MQ=255)                                                                                                                                                              aatatGCTGCTGCCGACCGGAACCAGCGTCGGTCTGACCAGCGTCCGCCTTGGCTTGAACCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTTCCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGAt                                                                    <  2:101267/249‑1 (MQ=255)                                                                                                                                                                 atGCTGTTCCCTACCGGAACCAGCGTCGGTCTGGCCAGCTTCAGCCTTGGCGTGAACCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAACCCTATCGCGCAGCCGCGTACCGGTGGCGATGCGCCCGATCATACTACGCCTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCGACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTTCTGATgg                                                                  <  2:262480/250‑1 (MQ=255)                                                                                                                                                                 atGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGCACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATgg                                                                  >  1:318751/1‑250 (MQ=255)                                                                                                                                                                         cccTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAag                                                               >  1:138472/1‑245 (MQ=255)                                                                                                                                                                           cTACCGGCACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGCGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCTATGCGCCCGCTCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCCGAAAAATGTGCTGCTGGAAGAGAtcgtc                                                       >  1:16335/1‑251 (MQ=255)                                                                                                                                                                            tACCGGCACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGCTCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGCACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGAtcgtcg                                                      >  2:64505/1‑251 (MQ=255)                                                                                                                                                                                    ccAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTAc                                              >  2:53060/1‑251 (MQ=255)                                                                                                                                                                                     cAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTAcc                                             <  2:36427/251‑1 (MQ=255)                                                                                                                                                                                                          cAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTg                        >  1:278850/1‑251 (MQ=255)                                                                                                                                                                                                                       aTCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCCGCCGCGTACCGGTGGAGATGCGCCCGAACAGACTACGACTATCTTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGt           <  1:10877/251‑1 (MQ=255)                                                                                                                                                                                                                        tCCGTGCAATGGGACCCAAAGGCGTTAGTCTGAGCGTTTTCACACCTATCGCTCCGCCGCGGACCGGCGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGACGGTCTGCTTTCCAGCAATCAGAAAGCTGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACAAACGACGCTGAAGTCGTTCTGGtt          >  2:63196/1‑251 (MQ=255)                                                                                                                                                                                                                                aTGGGACGCAAAGTCGTTCGTCTGAGCGTTTGCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCt  <  1:18588/251‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       GTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCT  >  NC_000913/2414602‑2415075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑