Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,111,259	Δ1 bp	coding (718/900 nt)	rfbD ←	dTDP‑4‑dehydrorhamnose reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,111,259	0	G	.	100.0%	97.4 / NA	22	coding (718/900 nt)	rfbD	dTDP‑4‑dehydrorhamnose reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (12/10);  total (12/10)

TCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCC  >  NC_000913/2111021‑2111508                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                          tcatcaTTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTg                                                                                                                                                                                                                                              >  2:143982/1‑251 (MQ=255)  catcatTCCTTTAAATTCACCTGGCTCCACCATCTCGAACAAGATGCAAAAACTAGTAAATTGCTTTAGTCGTAAACAATTCATTGAGCATTCGTTACACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATGTTTCTGTTTTAAGTCGAGAGTTATGTGGACGATGAGCTGGTGTAGGAGAGGCTGTTGTTGGTACTGCTTTGAGCTTGTTGAGTTCAAGGG‑AATGCCCGCtttt                                                                                                                                                                                                                                              <  2:191384/250‑2 (MQ=255)                          cTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGGGTGCAAGGG‑CATGCCTGCTTTGCGCGCCTCTTCAAACACCAgcgc                                                                                                                                                                                                                       >  2:160903/1‑249 (MQ=255)                           tCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCgcagc                                                                                                                                                                                                                    >  1:148269/1‑251 (MQ=255)                                         caaaaTGCAAAAACTATGAAATTGCTGTAGGCGTAAATAATTCATTGAGCAGTCGTTTCACGCCAACCTGCCAGTCATGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGGTAGGCTGTTGTTGGTACTGCGTTGAGCTGGTTGAGTGCAAGGG‑ATTGTCTGCGTTGCGCGCCTCTTCAAAAACCAGCGCATCATAATCGTACCAgg                                                                                                                                                                                                      <  2:58745/247‑1 (MQ=255)                                                cAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGt                                                                                                                                                                                               >  1:260497/1‑251 (MQ=255)                                                        aTTAAATGGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAACCGTACCAGGTTGTGGTACCACt                                                                                                                                                                                         <  2:148269/249‑1 (MQ=255)                                                                        cGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCCGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACa                                                                                                                                                                        >  2:268199/1‑250 (MQ=255)                                                                               aatTCATTGCGAATTCGTTTCACGACACCCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGCGATTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTCCCACTGGCTACCAAATGGTACAAGCCTGCg                                                                                                                                                                >  2:33432/1‑251 (MQ=255)                                                                                                      cccAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGac                                                                                                                                         <  2:242750/250‑1 (MQ=255)                                                                                                        caccatgcgaGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAAGTTTTCCGTATTAAGTCGAGATTTATGTGGACGAGTATGTGGTGTCGGATAGGCTGTTGTTGTTACTGCGTGGAGCTTGGTGAGTGCAAGTG‑AATGCCTGATTTGCGCGCTTCGTCAACTACCATCGCAGCCTAATCGTTTCAGGTTGTGTTACCACTGGCTTCCAACTGGCACACGCCTGCGACATCCGTTTTATTCatttcgccac                                                                                                                                       <  1:92025/242‑10 (MQ=255)                                                                                                                                 cgcAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATcagc                                                                                                              >  1:73469/1‑251 (MQ=255)                                                                                                                                       gTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGCCGAGCTGGTGTAGGATAGGCTGTTGTTGGTCCTGCGTTGCGCTTGTTGAGTGCAAGGG‑CATGCCTGCTTTGCGCGCCTCTTCAAACACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACACGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATcagccagc                                                                                                          >  1:239312/1‑249 (MQ=255)                                                                                                                                                       tCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGgc                                                                                        >  2:456224/1‑251 (MQ=255)                                                                                                                                                         tGTAGTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTTTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATCCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGgcg                                                                                       <  1:57737/250‑1 (MQ=255)                                                                                                                                                                   cGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGACCTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCCGCGCCGCATAATCGTACCAGGTTGTGGTACCACTGACTACCAACTGGAACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCCGTTCAGCACCTGTTGGCGCACCCAACTg                                                                             >  1:286350/1‑250 (MQ=255)                                                                                                                                                                      gagTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCg                                                                         <  1:375109/251‑1 (MQ=255)                                                                                                                                                                             gtgGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTtaata                                                                   >  2:172714/1‑250 (MQ=255)                                                                                                                                                                                       aGCTGGTGTAGGATAGGCTGTTGGTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCATGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAAttc                                                        <  2:73469/251‑1 (MQ=255)                                                                                                                                                                                           ggTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGGGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAAttcttc                                                     <  1:33432/250‑1 (MQ=255)                                                                                                                                                                                                            tgttgGGACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCCAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATACGCCAGCAGTTCAGAACCTGATGGCGCACCAAACTAATCGTTAATACCCGCTAATTCTTCACGCTCTTTTGCCAGACg                                   >  2:257232/1‑251 (MQ=255)                                                                                                                                                                                                                                 gttgAGTGCAAGGG‑AATGCCTGCGTTGTGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTTCCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCTGTTTATTCAGTGCGACACGAATGGCATGGGCTGTATAAACAGCCAGCAGTTCAGCTCCGGTTGGCGCATCAAACTGATCGTTAATAACCGCTAATTCTTCTCGCGCGTTTGCCAGATGTAACATCGTTTTGGTGAAGtt              <  2:286350/251‑1 (MQ=255)                                                                                                                                                                                                                                              ggAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTAtttccttttcc  >  1:116697/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                          TCATCATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCC  >  NC_000913/2111021‑2111508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑