Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	1,301,457	(CTGGGATAAT)1→2	coding (276/1632 nt)	oppA →	oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1301448 =	0 (0.000)	18 (0.400)	18/482	0.9	100%	coding (267/1632 nt)	oppA	oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	= 1301457	0 (0.000)					coding (276/1632 nt)	oppA	oligopeptide ABC transporter periplasmic binding protein

CATCAATAGCTTTCACGCCGAGATCGGTAATCGGTTTTTTCCCTTCAAGAATTTCATCAATACCGGCGATATGCCCATATTGCAGATAACTGGCATACGGAGAAGCAGTGTTCGGATCAACAGAACGTTGCCAGCTATACACAAAGTCTTGTGCTGTGACTGGCGTGCCATCAGACCATTTCGCATCTTTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTATCCCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1301680‑1301448 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCCTTCAAACAGGTCTCGGCTGATATTAGACTCCGGAACACCTTCAATTTTGTGCGGATCTAATGACTGAACTTCTGAACCATTGTTACGTACCAGTGTTTGTTTTTCCGCCAGTGTGACGCCTGCGGGTACATCAGCTGCCAGCGCGACATTCCCTGC  <  NC_000913/1301457‑1301239                                                                                                                                                                                                                                                                                                                                                                                                                                                                      CATCAATAGCTTTCACGCCGAGATCGGTAATCGGTTTTTTCCCTTCAAGAATTTCATCAATACCGGCGATATGCCCATATTGCAGATAACTGGCATACGGAGAAGCAGTGTTCGGATCAACTGAACGTTGCCAGCTATACACAAAGTCTTGTGCTGTGACTGGCGTGCCATCAGACCATTTCGCATCTTTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGC                                                                                                                                                                                                           >  2:162064/1‑251                      GATCGGTAATCGGTTTTTTCCCTTCAAGAATTTCATCCATACCGGCGATATGCCCATATTGCAGATAACTGGCATACGGAGAAGCAGAGTTCGGATCAACAGAACGTTGCCAGCTATACACAAAGTCTTGTGCTGTGACTGGCGTGCCATCAGACCATTTCGCATCTTTACGCAAATGGACGGGCCAGCCTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCCACGCCgggggatggataccc                                                                                                                                                                                      >  2:259882/1‑236                          GGTAATCGGTTTTTTCCCTTCAAGAATTTCATCAATACCGGCGATATGCCCATATTGCAGATAACTGGCATACGGAGAAGCAGTGTTCGGATCAACAGAACGTTGCCAGCTATACACAAAGTCTTGTGCTGTGACTGGCGTGCCATCAGACCATTTCGCATCTTTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTC                                                                                                                                                                                   >  1:129448/1‑250                                CGGTTTTTTCCCTTCAAGAATTTCATCAATACCGGCGATATGCCCATATTGCAGATAACTGGCATACGGAGAAGCAGTGTTCGGATCAACAGAACGTTGCCAGCTATACACAAAGTCTTGTGCTGTGACTGGCGTGCCATCAGACCAGTTCGCATCTTTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATC                                                                                                                                                                             <  1:422544/250‑1                                                              ACCGGCGATATGCCCATATTGCAGATAACTGGCATACGGAGAAGCAGTGTTCGGATCAACAGAACGTTGCCAGCTATACACAAAGTCTTGTGCTGTGACTGGCGTGCCATCAGACCATTTCGCATCTTTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCCTTCAAACAGGTCTCGG                                                                                                                                              >  2:209705/1‑251                                                                            CATATTGCAGATAACTGGCATACGGAGAAGCAGTGTTCGGATCAACAGAACGTTGCCAGCTATACACAAAGTCTTGTGCTGTGACTGGCGTGCCATCAGACCATTTCGCATCTTTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCCTTCAAACAGGTCTCGGCTGATATTAGACTC                                                                                                                                <  1:209705/251‑1                                                                                                 ACGGAGAAGCAGTGTTCGGATCAACAGAACGTTGCCAGCTATACACAAAGTCTTGTGCTGTGACTGGCGTGCCATCAGACCATTTCGCATCTTTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCCTTCAAACAGGTCTCGGCTGATATTAGACTCCGGAACACCTTCAATTTTGTG                                                                                                           <  1:393701/251‑1                                                                                                     AGAAGCAGTGTTCGGATCAACAGAACGTTGCCAGCTATACACAAAGTCTTGTGCTGTGACTGGCGTGCCATCAGACCATTTCGCATCTTTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCGACGACAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCCTTCAAACAGGGCTCGGCTGATATTAGACTCCGGAACACCTTCAATTTTGTACGG                                                                                                        >  2:12477/1‑250                                                                                                                              CGTTGCCAGCTATACACACAGTCTTGTGCTGTGACTGGCGTGCCATCAGACCATTTCGCATCTTTACGCAAATGTCAGGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCATTCAAACAGGTCTCGGCTGATATTATACTCCGGATCACCTTCAATTTTGGGCGGATCAAATGACTGAACTTCTGAACCAT                                                                              <  1:350413/251‑1                                                                                                                                         ATACACAAAGTCTTGTGCTGTGACTGGCGTGCCATCAGACCATTTCTCATCGTTACGCAAATGGAAGGGCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCCTTCAAACAGGTCTCGGCTGATATTAGACTCCGGAACACCTTCAATTTTGTGCGGATCTAATGACTGAACTTCTGAACCATTGTTACGTA                                                                     <  2:447240/249‑1                                                                                                                                                AAGTCTTGTGCTGTGACTGGCGTGCCATCAGACCATTTCGCATCTTTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCCTTCAAACAGGTCTCGGCTGATATTAGACTCCGGAACACCTTCAATTTTGTGCGGATCTAATGACTGAACTTCTGAACCATTGTTACGTACCAGTGTTT                                                            <  2:102177/251‑1                                                                                                                                                     TTGTGCTGTGACTGGCGTGCCATCAGACCATTTCGCATCTTAACGCAAATGAAAGGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCCTTCAAACAGGTCTCGGCTGATATTAGACTCCGGAACACCTTCAATTTTGTGCGGATCTAATGACTGAACTTCTGAACCATTGTTACGTACCAGTGTTTGTTTT                                                       >  1:164098/1‑251                                                                                                                                                          CTGTGACTGGCGTGCCATCAGACCATTTCGCATCTTTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGAGTCAGCGACGCCAGGTGCTGGATGAACGTCAAGATCGCTGACCAGTAAGACTTCAAACAGGTCTCGGCTGATATTAGACTCCGGAACACCTTCAATTTTGTGCGGATCTAATGACTGAACTTCTGAACCATTGTTACGTACCAGTGTTTGTTTTTCCGC                                                  >  1:243798/1‑251                                                                                                                                                              GACTGGCGTGCCATCAGACCATTTCGCATCTTTACGCAAATGGAAGGGCCAGACTGTCGCGTCTTTATTATCCCAGATTACCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCCTTCAAACAGGTCTCGTCTGATATTAGACTCCGGAACCCCTTCAATTTTGTGCGGATCTAATGACTGACCTTCTGAACCATTTTTACGTACCATTGTTTGTTTTTCCGCCAGT                                              <  2:34798/251‑1                                                                                                                                                                        CCATCAGACCATTTCGCATCTTTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTAGCCCAGATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCCTTCAAACAGGTCTCGGCTGATATTAGTCTCCGGAACACCTTCAATTTTGTGCGGATCTAATGACTGAACTTCTTAACCATTGTTTCGTACCAGTGTTTGTTTTTCCGCCAGTGTGACGCCTG                                    <  1:172088/251‑1                                                                                                                                                                                   TTTCGCATCTTTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCCGTAAGCCTTCAAACAGGTCTCGGCTGATATTAGACTCCGGAACACCTTCAATTTTGTGCGGATCTAATGACTGAACTTCTGACCCATTGTTACGTACCAGTGTTTGTTTTTCCGCCAGTGTGACGCCTGCGGGTACATCg                         >  2:14134/1‑250                                                                                                                                                                                             TTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCCTTCAAACAGGTCTCGGCTGATATTAGACTCCGGAACACCTTCAATTTTGTGCGGATCTAATGACTGAACTTCTGAACCATTGTTACGTACCAGTGTTTGTTTTTCCGCCAGTGTGACGCCTGCGGGTACATCAGCTGCCAGC                <  1:167225/250‑1                                                                                                                                                                                                          atGTCCAGACTTTCGCGTCTTTATTATCCCAGATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCCTTCAAACAGGTCTCGGCTGATATTATACTCCGAAACACCTGCAATTTTGTGCGGATCTAATGACTGAACTTCTGAACCATTGTTACGTCCCAGTGTTTGTTTTTCCGCCAGTGTGACGCCTGCGGGTACATCAGCTGCCAGCGCGACATTCCCTGC  <  2:61097/249‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                      CATCAATAGCTTTCACGCCGAGATCGGTAATCGGTTTTTTCCCTTCAAGAATTTCATCAATACCGGCGATATGCCCATATTGCAGATAACTGGCATACGGAGAAGCAGTGTTCGGATCAACAGAACGTTGCCAGCTATACACAAAGTCTTGTGCTGTGACTGGCGTGCCATCAGACCATTTCGCATCTTTACGCAAATGGAAGGTCCAGACTTTCGCGTCTTTATTATCCCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1301680‑1301448 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTATCCCAGGATTCAGCGACGCCAGGTGCTGGATGACCGTCAAGATCGCTGACCAGTAAGCCTTCAAACAGGTCTCGGCTGATATTAGACTCCGGAACACCTTCAATTTTGTGCGGATCTAATGACTGAACTTCTGAACCATTGTTACGTACCAGTGTTTGTTTTTCCGCCAGTGTGACGCCTGCGGGTACATCAGCTGCCAGCGCGACATTCCCTGC  <  NC_000913/1301457‑1301239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.