Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,207,549	Δ1 bp	coding (179/606 nt)	ttdB →	L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,207,549	0	G	.	100.0%	332.4 / NA	71	coding (179/606 nt)	ttdB	L(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (37/34);  total (37/34)

CGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCAC  >  NC_000913/3207306‑3207796                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                         cgcgGCCCGCGGCTGGTTCAGGCCGATCGCCCCTTGGAAAATCGGCCCCACACCCGGAGCGCGTTATGAAAAAGATCCCGACAACCCCGAGCAAGGCTGAAGAGCTGCAAGATATGCGCGCCGGCGATGTGATCTCCCTGACCGGTACGCGGTTGACCGGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCg                                                                                                                                                                                                                                                  <  2:55621/251‑1 (MQ=255)  gcgGCACGCTGCTGGTTCATGCCGATCTCACGTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGCAACCGATCCTGACAACCCCGATCAAAGCTGAATCTCTGCCAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGCTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGACACGTCCGATCCCTTACGATCTCAACTGCAAAGCGATTTTCCACGCTT‑CCCCATCGt                                                                                                                                                                                                                                                 >  1:245916/1‑251 (MQ=255)    ggCACGCTGGTGGTTCATGCCGATCTCACCTTTAAAAATCGGTCTCACACCCGGGGCGCTTGAAGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATTTTCGCGTCGGCGATGTGATCTACCTGGCCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCATGCTG‑CCCCAGCGTgc                                                                                                                                                                                                                                               <  2:276222/251‑1 (MQ=255)           tgctgGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCaaaa                                                                                                                                                                                                                                         >  1:234291/1‑250 (MQ=255)               gggtaatcccGATCTCACATTTGAAATGCTGTCTCACCCCCGGAGCGCTTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTCCCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACgg                                                                                                                                                                                                                                     <  2:317553/242‑1 (MQ=255)                  tCATGCCGATCTCCCCTTTGAAAAGCTGTCTCACACCCGTAGCGGGTTATGAAAAAGATCCTGACAACCCCGATAAAAGCGGAAGATCTGCAAGATGTTCGCGTCGGCGATGGGATCGACCTGACCGGTACGCTGGTGACCTGCCGCGCC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCGTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGa                                                                                                                                                                                                                                  <  2:373472/250‑1 (MQ=255)                       ccGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATg                                                                                                                                                                                                                            >  1:146590/1‑251 (MQ=255)                        cGATCTGACCTTTGGAAATCTGTCTCACACCCGGAGCGCGTTATGAAACAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGCTGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGACCGATCCCTTGCGGTCTCAACGGGAAAGCGATTTTCCACGCTG‑CCCATTGGTGCGCAAAAAAGGCGACAAATgg                                                                                                                                                                                                                           >  2:56037/1‑251 (MQ=255)                              cACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATg                                                                                                                                                                                                                     <  1:125034/251‑1 (MQ=255)                               aCCTTTGAAAATCTGTCTCACACCCGGAGCGAGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATgg                                                                                                                                                                                                                    >  1:135458/1‑251 (MQ=255)                                   ttGAAAATCTGTCCCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGCCCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGtc                                                                                                                                                                                                                  <  1:365700/249‑1 (MQ=255)                                                tcACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCCAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGAc                                                                                                                                                                                                    >  1:218209/1‑250 (MQ=255)                                                  acacCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCACAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAc                                                                                                                                                                                                 >  1:288483/1‑251 (MQ=255)                                                     cccGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCGGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAg                                                                                                                                                                                              <  2:146590/251‑1 (MQ=255)                                                                  aTGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGCCCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGa                                                                                                                                                                                 <  1:411545/250‑1 (MQ=255)                                                                      aaaaGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGtt                                                                                                                                                                            >  1:369198/1‑251 (MQ=255)                                                                       aaaGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGttt                                                                                                                                                                           >  1:50656/1‑251 (MQ=255)                                                                         aaaTCCTGCCACCCCCGATCAAAGCTGAAGATCGGCAAGATATTCGCGTCGGCGATGTAGTCTACCTGTCCGGTACGCTGGTGCCTTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGATCTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCTATTTTCCACGCTG‑CCCCATCGGTCGCAAAAACGGCGACAAATGGGAGATGGGCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTg                                                                                                                                                                         <  2:283321/249‑1 (MQ=255)                                                                            cccTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATTTTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGCC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAAc                                                                                                                                                                      <  2:234291/250‑1 (MQ=255)                                                                             ccTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAAc                                                                                                                                                                      <  1:127648/250‑1 (MQ=255)                                                                                gACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCTTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGt                                                                                                                                                                    <  2:139820/249‑1 (MQ=255)                                                                                gACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTg                                                                                                                                                                   >  1:160893/1‑250 (MQ=255)                                                                                   aaCCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGtc                                                                                                                                                                                                                  >  1:283615/1‑201 (MQ=255)                                                                                     ccccGAACAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTa                                                                                                                                                             <  1:143181/251‑1 (MQ=255)                                                                                            cAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGc                                                                                                                                                       >  1:380854/1‑250 (MQ=255)                                                                                            cAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCGGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGc                                                                                                                                                       <  1:232133/250‑1 (MQ=255)                                                                                              aaGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGc                                                                                                                                                       >  1:95161/1‑248 (MQ=255)                                                                                                gcttaaGATCTGCAAGATATTAGAGTCGGCGATGTTATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGACCTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAACAACGGCGACAAATGGGAAATGGTCTCCGTCGGCCCGCCAACCAGTATGC‑GTATGGAAAGTTTTGAAAGCGAATTTATTGAGAAGAcc                                                                                                                                                  >  2:276652/5‑251 (MQ=255)                                                                                                gCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGAcc                                                                                                                                                  <  1:391792/251‑1 (MQ=255)                                                                                                 gtGAAGATCTGCAAGATATTCGCGTCGGCGATTTGATCTTCCTGACCGGTACGCTGGTGACCTGCCGCTAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCg                                                                                                                                                 <  1:134767/250‑1 (MQ=255)                                                                                                 ccctatccatGCAAGATATTCGCGTCGGCGATGTGATCTACCGGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGGATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCg                                                                                                                                                 <  1:177016/242‑1 (MQ=255)                                                                                                 cTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCACAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCg                                                                                                                                                 >  1:199648/1‑251 (MQ=255)                                                                                                   gAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAAc                                                                                                                                                                      >  1:444853/1‑228 (MQ=255)                                                                                                   gAAGATCTGCAAGATATTCGCGCCGGCGATGTGACCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAAc                                                                                                                                                                      <  2:444853/228‑1 (MQ=255)                                                                                                    aaGATCTGCAAGATATTCGCGTCGGCGATGTGATCGACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCg                                                                                                                                              >  1:137467/1‑251 (MQ=255)                                                                                                         cTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGGGAATTTATTGAGCAGCCCgagatcggc                                                                                                                                         >  2:177016/1‑243 (MQ=255)                                                                                                             aaGATATTCGCGTCGGCGATGTGATTTACCTGACCGGTACGCTGGTGCCCTGCCGCGAC‑GTTTGTCTCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACtgg                                                                                                                                     <  1:229178/251‑1 (MQ=255)                                                                                                              aGATATTCGCGTCGGCGAGTTGATCTCCCTGACCGTTCCGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCTCGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACtggt                                                                                                                                    <  1:198134/251‑1 (MQ=255)                                                                                                                atatTCGCTTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACTGATTG‑CACCGACGTTTGATCGCACTGAAACGTTCGATCCCTTACGATCTCAACGGCAAATCGATTTTCCACGCTG‑CCCCCTCGTGCGCAAACGCGGCGCCAAATGGGAGATGGTCTCCGTCGGCCAGCCAACCAGTA‑GCAGTATGGGCAGATTTAAACGTGAAGTGATTGAGCAGCCCGGCGTgctccgggtgg                                                                                                                                  >  2:5452/1‑241 (MQ=255)                                                                                                                     cgcgTCGGCGATGTGATCTCCCTGACCGGTACGCTGGCGACCTGCCGCGCC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCCCGCTG‑CCCCATCGCGCGCAAAAACGGCGACACATTGGCGATGGTCTCCGTCGCCCCGCCAACCCGTATGC‑GTATGGAACGTTTTGAACGTGAATTTATTTAACCGACCGGCGTGCAACTGGTGGTTggg                                                                                                                             >  2:2629/1‑250 (MQ=255)                                                                                                                           ggCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTACACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggg                                                                                                                       >  2:451062/1‑250 (MQ=255)                                                                                                                                   gaattaccggcccGGTACGCTGGTGTCCTGCCGGTAC‑GGCTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCACAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGATATGGTCTCCGTCGGCCCTACAACCAGTATGC‑GTATGGATAGTTTGGAACGTGAATTTATTGAGCAGACCGGCGTGAATCTGGTGGTTGGCAAAGGTGCTAttg                                                                                                                <  2:282022/239‑3 (MQ=255)                                                                                                                                        aCCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGaa                                                                                                                                                                 >  2:128521/1‑196 (MQ=255)                                                                                                                                        aCCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGaa                                                                                                                                                                 <  1:128521/196‑1 (MQ=255)                                                                                                                                                ggcccGCTGGTGCCCTCCCGCTCC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTAGGC‑GTATGGAAAGTTGTGAACGTTAATGTATTGAGCAGACCGGCGTGAAACAGTTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCgaa                                                                                                  <  2:470445/247‑1 (MQ=255)                                                                                                                                                    cGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCACTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCgaaga                                                                                                >  1:50538/1‑249 (MQ=255)                                                                                                                                                           gacgtGCCGCGAC‑GTTTGGCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGcc                                                                                        <  2:167192/246‑1 (MQ=255)                                                                                                                                                            aCCTGCCGCGAC‑GTTTGCCACCGACGTTTGATCGCACTGCAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCTCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GAATGGAAAGTTTTGAACGTGACTTTATTGAGCAGACCGGCGTGAAACAGGTGGTTGGCAACGGTGGTATGGGGCCGCTGACCGAAAAAGGCTGcc                                                                                        >  2:194941/1‑249 (MQ=255)                                                                                                                                                                     gAC‑GTTTGTCACCGCCGCTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCACGACAACCAGTATGC‑GAATGGAAAGTTTTGAACGTGACTTTATTGAGCAGACCGACGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCACTGACCGCAGAAAGCTGCCAGAAATTcc                                                                               >  2:34220/1‑248 (MQ=255)                                                                                                                                                                       cgacgcccttccgatctttGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCaa                                                                              <  2:42751/233‑1 (MQ=255)                                                                                                                                                                          tttGTCACCGCCGGTTGATCGAACTGAAACGGCCGATCCCTTACGATCTCAACGGCAGAGCGATTTTCCACGCTG‑CCCCCTCGTGCGCAAAAACGGCGACAAAAGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAATTTTGAACGTGAATTTATCGAGCAGACCGGCGTGAAACTGGAGGTTGGCAAAGGTGGGATTGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCACGgcgc                                                                         >  2:17987/1‑251 (MQ=255)                                                                                                                                                                               cACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAt                                                                    >  1:377930/1‑251 (MQ=255)                                                                                                                                                                                  cgccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAtgtg                                                                 <  2:380854/251‑1 (MQ=255)                                                                                                                                                                                    ccgTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTTAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAGGTGAt                                                               <  2:447464/251‑1 (MQ=255)                                                                                                                                                                                      gTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGAttt                                                             >  2:158994/1‑251 (MQ=255)                                                                                                                                                                                        ttGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGCTTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGAGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCaa                                                                              >  1:42751/1‑232 (MQ=255)                                                                                                                                                                                           gtCGAACTGAAACGGCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCg                                                        <  2:106400/250‑1 (MQ=255)                                                                                                                                                                                                   gAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGTAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGGGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTg                                                >  1:298277/1‑251 (MQ=255)                                                                                                                                                                                                    aaaCGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAAGTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTgc                                               <  2:430492/251‑1 (MQ=255)                                                                                                                                                                                                     aaCGTACGCTCCCTTATGATCTCAACGGTCAACCGATTTTCCAAGCTC‑CCCAATCGTGCGCAAAAACGGCGAAAAATGGGGGACGGTATCCGTCGGCGCGAAAACCAGTATGC‑GTATGGCAAGATTTGAACGTGACTTTATTGAGCAGACCGGCGTGAAAATGGTGGTTGGCAAAGGAGGTATGGTGCCGCTGACCGAAGCAGGCTGCCAGACATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTgcg                                              >  2:455881/1‑251 (MQ=255)                                                                                                                                                                                                        gTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGCCAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCCGGCTGCGCgg                                           >  2:29369/1‑251 (MQ=255)                                                                                                                                                                                                            gATCCCTTACGATCTCAACGGCAAAGCGATTTTCCCCGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCt                                       <  2:56370/251‑1 (MQ=255)                                                                                                                                                                                                               cccTTACGAGCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGTCCGTCGGGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATTTGATTTTCCCGGCAGGCTGCGCGGTGCTgg                                     <  1:173779/250‑1 (MQ=255)                                                                                                                                                                                                                    aCGACCTCTACGGCAAAGCGATTTTCCACGCTT‑CCCTATCGTGCGCAAAAACGTCGACAAATGGGAGATGGTCTCCTTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGCGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCa                                <  1:396569/250‑1 (MQ=255)                                                                                                                                                                                                                           cAACGGCAAAGCGATTTTCCACGCTG‑CCCCTTCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑TTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAg                          <  1:4195/249‑1 (MQ=255)                                                                                                                                                                                                                              cGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTgg                      >  1:76155/1‑250 (MQ=255)                                                                                                                                                                                                                               ggCAAAGCGATTTTCCCCGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGCGATGGTCTCCGTCGGCCCGCCCACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGCAGAAGGCTGCCAGAAATTCAAGGCGCTAAATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGa                     >  2:331174/1‑250 (MQ=255)                                                                                                                                                                                                                                    aGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAag                   >  1:33874/1‑247 (MQ=255)                                                                                                                                                                                                                                     gCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGATGCGCGGTGCTGGCGGCCAACCAGGTGGAAGAGAtt              >  2:351698/1‑251 (MQ=255)                                                                                                                                                                                                                                             ccACGCTG‑CCCCATCGTGCGCAAAACCGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGATTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTACCGGCGGGCTGCGCGGGGCTGGCGGCAACCCAGGGGGAaggagttgacgaagg      >  2:304538/1‑238 (MQ=255)                                                                                                                                                                                                                                             ccACGCTG‑CCCCATCGTACGCAAAAACGGCGACAAATGGGAGATGTTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGATCGTGAAGTTATTGAGCAGACCGGCGTGAAACTGGTTGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAATAAGGCTGCCAGAAATTCAAGGCGCTGCATTTGATTTTCCCGGCAGGCTGCGCGGTGTTGGCGGCAACCCAGGTGGAAGATATTgaagaat       <  2:446575/250‑2 (MQ=255)                                                                                                                                                                                                                                                  gctgCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGTTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCAc  <  1:266733/248‑1 (MQ=255)                                                                                                                                                                                                                                                  gctgCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGGGCAc  >  2:180136/4‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                         CGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGAC‑GTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGC‑GTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCAC  >  NC_000913/3207306‑3207796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑