Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	225.3 / NA	67	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (36/31);  total (36/31)

AATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGT  >  NC_000913/3387796‑3388283                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      aaTGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACcgg                                                                                                                                                                                                                                                  >  2:86202/1‑251 (MQ=255)    aaTCAGCGCGGTATAACCGACCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTGCAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAAGGATGATCACCAGGCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACTGTTCACCTCCCGCAGCAAAGGCCGGACcggcgg                                                                                                                                                                                                                                               <  1:286965/250‑1 (MQ=255)     aTCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCggcggc                                                                                                                                                                                                                                              <  2:397236/251‑1 (MQ=255)     aTCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGAGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTAACCTCCCGCAGCAAAGGCCGGACCggcggc                                                                                                                                                                                                                                              >  2:333011/1‑251 (MQ=255)                   aCCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTg                                                                                                                                                                                                                                >  2:174578/1‑251 (MQ=255)                                   gCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGCCAGTACCGCCCAGCg                                                                                                                                                                                                                >  2:255170/1‑251 (MQ=255)                                         gAGTTTTCTATTCGTACCAGCGAGGATATCCCGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCACTAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGt                                                                                                                                                                                                          >  1:155439/1‑251 (MQ=255)                                             tttCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTc                                                                                                                                                                                                       <  1:272482/250‑1 (MQ=255)                                               tCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCa                                                                                                                                                                                                     <  2:299645/250‑1 (MQ=255)                                                    tCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTgg                                                                                                                                                                                                <  1:269626/250‑1 (MQ=255)                                                     cGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGaa                                                                                                                                                                                              <  1:54082/251‑1 (MQ=255)                                                       tACCAGCGAGGATATCCAGGTACCAAAAACGGCCCAGATCCAGCACACCAGAATCATCAATAATGGAGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGAGCAAACAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGACAAGACCGGCGGCAACAAACGCCGCTGTCAGTACCGCCCAGCGTGGAGTTTCCAGATGGacgt                                                                                                                                                                                            >  2:185185/1‑248 (MQ=255)                                                           aGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGCATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGaa                                                                                                                                                                                         >  1:41535/1‑250 (MQ=255)                                                               aGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGcc                                                                                                                                                                                     >  1:412165/1‑250 (MQ=255)                                                                ggATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCcaa                                                                                                                                                                                   <  2:422180/251‑1 (MQ=255)                                                                   tatCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCcaaca                                                                                                                                                                                 >  1:250682/1‑250 (MQ=255)                                                                      ccAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGGTTCCAGCTGGAAGTGAAAGCCaagaaaca                                                                                                                                                                             <  1:101165/251‑1 (MQ=255)                                                                                   ccGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAAt                                                                                                                                                                >  1:351093/1‑251 (MQ=255)                                                                                    cGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAAGACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTGCCAGCTGTAATTGAAAGCCAACAAACAGCGCCAGTACAATg                                                                                                                                                               <  2:418375/251‑1 (MQ=255)                                                                                     ggCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATgg                                                                                                                                                              <  2:412237/251‑1 (MQ=255)                                                                                      gCCCAGATACAGCACAACAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGc                                                                                                                                                             <  1:120571/251‑1 (MQ=255)                                                                                       cccAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCg                                                                                                                                                            <  1:255170/251‑1 (MQ=255)                                                                                        ccAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCgg                                                                                                                                                           <  2:234994/251‑1 (MQ=255)                                                                                                      ccAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTAcc                                                                                                                                            <  1:170391/251‑1 (MQ=255)                                                                                                       cAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCCCCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTGCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCg                                                                                                                                           <  1:8902/251‑1 (MQ=255)                                                                                                         gAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCa                                                                                                                                         <  1:333011/251‑1 (MQ=255)                                                                                                             catcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGc                                                                                                                                     <  1:372466/251‑1 (MQ=255)                                                                                                               tcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCCGTTTTAACGCAAAg                                                                                                                                      >  1:401232/1‑248 (MQ=255)                                                                                                               tcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAg                                                                                                                                      <  2:401232/248‑1 (MQ=255)                                                                                                                 aataatGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAat                                                                                                                                 <  2:134317/251‑1 (MQ=255)                                                                                                                         tGCGCGGATCATCGCACTGATGATCCCCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAATAAGCCACGATAGCGAATAGCGCCAGAATAAGGTTCACCTCCCGCAGCAAAGGCCGGACAGGCGGCAACAATAGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGACAGTACAATGGCGGT‑CGCCAGTTTATCCGCAAAGAGTATATGtttgt                                                                                                                         >  2:458973/1‑248 (MQ=255)                                                                                                                             cgGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGC‑CCCCAGGTTTTCCGCAAAACGAAAATGTTGGTTA‑gg                                                                                                                    >  2:465375/1‑250 (MQ=255)                                                                                                                               gATCATCGCCATGATGATCCCCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCCACAAACAGCGCCAGTACGATGGCGGT‑CGCCCGTTTTTCCGCAAAGCGAATATTTTgggtaact                                                                                                                    >  2:108101/1‑244 (MQ=255)                                                                                                                               gATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCaa                                                                                                                  <  1:27594/251‑1 (MQ=255)                                                                                                                                 ccagcGCAAAGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCTGCAACAATCGCCGCTGTCAGTACCGCCCAGCTTGGCGTTTCCAGCGGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCCGT‑CGCCAGTTTTACCTCAAAGCGAATATGTTGGTTA‑GCAATg                                                                                                                <  2:218549/247‑1 (MQ=255)                                                                                                                                      gCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑AGACAGTTTTACCGCCAAGCGAATATGTTGGGTA‑GCAAAGgagg                                                                                                            >  2:350343/1‑249 (MQ=255)                                                                                                                                              gatCCCCAGTCCGGCAATACAGCAAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTGGGTTA‑GCAATGGAGAAAATA‑CCCa                                                                                                  <  2:27066/251‑1 (MQ=255)                                                                                                                                              gatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACCAACAGCGCCAGTACAATGGCGGT‑CGCCCGTTTTACCCCCAAACGAATATGTTGGTTA‑GCAATGGGGAAAATA‑ccc                                                                                                   >  2:385614/1‑250 (MQ=255)                                                                                                                                               acaaCCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCGCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCAt                                                                                                 <  2:240169/248‑1 (MQ=255)                                                                                                                                                 cACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAACAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCCGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCCGTTTTTACGCACAGCGACAATGTTGGGTA‑GCAATGGGGAAAATAACCCATc                                                                                                >  2:68683/1‑251 (MQ=255)                                                                                                                                                  aCCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGt                                                                                              <  1:68683/251‑1 (MQ=255)                                                                                                                                                   ccAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGGATATGTTGGGTA‑GCAATGGGGGACATA‑CCCATCGTg                                                                                             >  2:398278/1‑251 (MQ=255)                                                                                                                                                    cAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGa                                                                                            <  2:44025/251‑1 (MQ=255)                                                                                                                                                           gCAATACAGCCATAAAATTGGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGGATACGGTTGACCGCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTGTCCAGCTGGAAGTGAAAGCCAACAAACAGCTGCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAAGATGGTGGTTA‑GCTGTGGAGAAAATA‑CCTATCTTGATGAACCa                                                                                     <  2:221209/251‑1 (MQ=255)                                                                                                                                                                  aGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGAGAGCGAATAGCGCCAGAATACGGGTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCAc                                                                              <  2:8401/251‑1 (MQ=255)                                                                                                                                                                   gCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGAGCATATGGTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTTGTTAACCCAACtac                                                                               >  1:427637/1‑247 (MQ=255)                                                                                                                                                                         aaaTGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCCGTTTTTCCGCCAAGCGAATATATTGGTTT‑GCAATGGGGAAAATA‑CCCATCGTGATTTACCaaaaccccccaggcg                                                                       >  2:66087/1‑239 (MQ=255)                                                                                                                                                                          aaTGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCCAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGATTaa                                                                                        >  1:307368/1‑233 (MQ=255)                                                                                                                                                                          aaTGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGATTaa                                                                                        <  2:307368/233‑1 (MQ=255)                                                                                                                                                                             gcgCCGATTATGCGCAAAAAGTCACGATATCGAATAGCGCCAGAATACGGTTCACCTACCGCAGCAAAGGCCGGACCGTCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCTCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATT‑CCCATCGTGAGTAACCAAACTCACGCAGGCGATgg                                                                   <  2:383001/249‑1 (MQ=255)                                                                                                                                                                                  gatgatGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGATTAAACAAACTCACGCAGGCGATGGGCCAt                                                              >  1:158770/1‑251 (MQ=255)                                                                                                                                                                                         cgcAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCCGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑ACCATCGTGATTAACCAAACTCACGCAGGCGGTGGGCCCTTTTACgg                                                       >  2:174880/1‑251 (MQ=255)                                                                                                                                                                                           cAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGgaa                                                     <  1:69249/251‑1 (MQ=255)                                                                                                                                                                                            aaaaaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTAACGCAAAGCGAATATGGTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGCAGGGCCATTTTACggga                                                     >  1:40550/1‑248 (MQ=255)                                                                                                                                                                                                   cACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAACGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCTCCCAGAGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGG‑CGCCAGGTTTTCCGCAAAGCGAATATGTTTGGTA‑GCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGACGGCGATGGGCCATTTTACGTAAGAACGAAt                                             >  1:36186/1‑251 (MQ=255)                                                                                                                                                                                                    aCGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAAAGAATc                                            >  1:65469/1‑251 (MQ=255)                                                                                                                                                                                                       aTAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAAAGAATCCTg                                         >  1:440371/1‑251 (MQ=255)                                                                                                                                                                                                                  cgcCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGGTTTACCGCAAAGCGCATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCCTCGTGATTAACCAAACTCACGCAGGCGGTGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTcgc                              >  1:23410/1‑251 (MQ=255)                                                                                                                                                                                                                      aGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTcgcgctc                          >  1:363105/1‑248 (MQ=255)                                                                                                                                                                                                                             ggTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCCGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑ACCATCGGGATTAACCAAACTCACGCAGGGGATGGGCCATTTTACGGAAGAACCAATCCTGGCTTTCGTCGCGCTCTTGTTTg                   >  1:19885/1‑251 (MQ=255)                                                                                                                                                                                                                                 cACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCa                <  1:466224/250‑1 (MQ=255)                                                                                                                                                                                                                                   ccTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACCACCAGCGCCAGTACAATGGCGGC‑GGCCAGTTTTACCGCAAAGAAAATATGTTGGTTA‑GCCAGGGGGAAAATA‑CCCACTGTGTTTAAACAAACTCACGCAGGACATGGGCCATTTTACGGAAGACCAAATACTGGCTTTCGTCGCGGACTTGTTTTCCaatg             >  1:270286/1‑248 (MQ=255)                                                                                                                                                                                                                                   ccTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTACCGCTGGAAGTGAAAGACAACAAAAAGCGCCAGTACAATGGCGGTACCCCGGTTTT‑CCCCAAAGCGAATATGTTGGATT‑GCAATGGAGAAAAAA‑ACCATCGTGATTAACCAAACTCACGCAGGCGATGGGCACTTTTACGGGAGAACGACTCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTg             >  1:463376/1‑251 (MQ=255)                                                                                                                                                                                                                                     tCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCCGTACAATGGCGGT‑ACCCAGTTTTTCCGCCAAGCGAATATGTTGGTTATGCA‑GGGAGGAAATA‑CCCATCGTGATTAACCAAACTCCCCCCGGACATGGGCCATTTTACGGAAGAACGAAAACTGGCTTTCGTCCCGAGCTTGATTGCAAGttgc           >  2:334799/1‑248 (MQ=255)                                                                                                                                                                                                                                       ccGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGGGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCAATGGGCCATTTTACGGAAGAACGAATCATGGCTTTCGTAGCGATCTTGTTTTCCAGTGaccc         >  1:126445/1‑250 (MQ=255)                                                                                                                                                                                                                                        cGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGG‑CACCCGCTTTACCGCCAACCGAAAATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGATTAACCCAACTCACGCCGGGGATGGGGCATTTTAAGGAAGAACGAATCCTGGGTTTCGTCGCGGTCTTGTTTGCCaagtaccac        >  1:15743/1‑243 (MQ=255)                                                                                                                                                                                                                                                aGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑AGCCAGTTTTACCGCAAAGCGAATATGTTTGTTA‑GCAATGGAGAAAAAA‑CCCAACGTGGTTAACCAAACTCACCGAAGCGATGGGGCATTTTTCGGGAGAACGAATCCTGGCTTTCGGCGCCGTCTTGGTTGCCAGTGGccaccaatgg  >  2:136425/1‑245 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      AATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGT‑CGCCAGTTTTACCGCAAAGCGAATATGTTGGTTA‑GCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACCACTGT  >  NC_000913/3387796‑3388283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑