Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,597,677	T→C	intergenic (‑117/‑307)	livK ← / → panZ	L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein/PanD maturation factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,597,677	0	T	C	100.0%	108.7 / NA	30	intergenic (‑117/‑307)	livK/panZ	L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein/PanD maturation factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/15);  total (15/15)

ATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGTATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAATAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAATTTTGTAATTATTGCTGTTAAAAAATTAATCACCTGCC  >  NC_000913/3597432‑3597902                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                  atCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATCGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCAtttt                                                                                                                                                                                                                              >  2:383408/1‑251 (MQ=255) atCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCAtttt                                                                                                                                                                                                                              >  1:148094/1‑251 (MQ=255)   cGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTtgt                                                                                                                                                                                                                            <  2:334221/251‑1 (MQ=255)                                    cGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTc                                                                                                                                                                                           <  2:148094/251‑1 (MQ=255)                                    cGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTc                                                                                                                                                                                           <  1:383408/251‑1 (MQ=255)                                          cGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTaa                                                                                                                                                                                     <  1:95797/251‑1 (MQ=255)                                               aCTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACa                                                                                                                                                                                <  1:203512/251‑1 (MQ=255)                                                                cATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAAttt                                                                                                                                                               <  2:39229/251‑1 (MQ=255)                                                                 aTAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAAttt                                                                                                                                                               >  1:4126/1‑250 (MQ=255)                                                                                                   cTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGata                                                                                                                                                                                                                        >  2:343353/1‑159 (MQ=255)                                                                                                   cTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGata                                                                                                                                                                                                                        <  1:343353/159‑1 (MQ=255)                                                                                                    tGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGAt                                                                                                                                                                                                                <  1:179329/166‑1 (MQ=255)                                                                                                    tGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGAt                                                                                                                                                                                                                >  2:179329/1‑166 (MQ=255)                                                                                                       gatgatAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTACGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCAt                                                                                                                         >  2:128608/1‑250 (MQ=255)                                                                                                         tgatAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATaa                                                                                                                       >  1:156803/1‑250 (MQ=255)                                                                                                           atAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATACCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAAt                                                                                                                    >  2:420150/1‑251 (MQ=255)                                                                                                                   cGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGcac                                                                                                            <  2:4126/251‑1 (MQ=255)                                                                                                                          cGTTTGATAGTCAAAATTAACCATTCGTGATGTTGTGTTGCTTTGTTTTAATGTGTTAACAAATCATACTGTTCTTTTTTTATACTGCTCTGTTTTTGCCTGACTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTCTTTTTCAGGCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAggg                                                                                                      >  2:63895/1‑250 (MQ=255)                                                                                                                                                aTTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCTATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAATAATTCACTATCATTc                                                                                 >  1:240874/1‑249 (MQ=255)                                                                                                                                                        tgttgtgttgCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAATAATTCACTATCATTCAGGGGATTAt                                                                       >  1:301093/1‑251 (MQ=255)                                                                                                                                                              gttgCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAATAATTCACTATCATTCAGGGGATTATGCTGGa                                                                 <  1:25312/251‑1 (MQ=255)                                                                                                                                                              gttgCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAATAATTCACTATCATTCAGGGGATTATGCTGGa                                                                 <  1:172657/251‑1 (MQ=255)                                                                                                                                                                      gTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAATAATTCACTATCATTCAGGGGATTATGCTGGACAttttt                                                          <  1:247943/250‑1 (MQ=255)                                                                                                                                                                            aTGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAATAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATtctc                                                   <  2:156803/251‑1 (MQ=255)                                                                                                                                                                               tgtTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCt                                                                                                                                   >  2:335996/1‑168 (MQ=255)                                                                                                                                                                               tgtTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCt                                                                                                                                   <  1:335996/168‑1 (MQ=255)                                                                                                                                                                                   aaCAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAATAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGtt                                            <  2:147334/251‑1 (MQ=255)                                                                                                                                                                                       aaTCAGAATGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTCGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGATCAGATAATATTCTGAATTTATTGATAGATAAACAGAAACAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAATAATTCACTATCATTCAGTGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTat                                        >  2:13107/1‑250 (MQ=255)                                                                                                                                                                                                                         ttGCCTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGCTAGATAAACAGAAAACAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGCATAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTATTTTTGTAATTATTGCTGTTAAAAAATTAATCAcc      >  2:284457/1‑251 (MQ=255)                                                                                                                                                                                                                             cTGTCTGATTAAGGGGTTAGCGCAGCATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAATAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAATTTTGTAATTATTGCTGTTAAAAAATTAATCACCTGcc  >  2:130011/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                  ATCGCCCCACTGGGCAATCGGGCCGGACATCGCGCCGACAACGGCGACTTTAATATCGTCAGCCATAGCGGTGTGTGAAATTGCCAGTGCAATCATCCCTGCGATGATAGTTTTCGCATTCCGTTTCATAGTCAAAAATCCCCATTCGTGATGTTGTGTTGCTTTGTTTTTATGTGTTAACAAATCAGACTGTTCTTTTTTTATACTGCACTGTTTTTGCCTGTCTGATTAAGGGGTTAGCGCAGTATTTTGTGATAATAGCGATTAAAATCCCTATTTTTCAGTCGATTAAGAACAGATAATATTCTGAATTTATTGATAGATAAACAGAAAAAAGTGCCTTTGTCAGCATAAAATAACGGCACAAAGGGCGGAATAATTCACTATCATTCAGGGGATTATGCTGGACATTTTTCATTCTCTAATGTTTTAATTTTGTAATTATTGCTGTTAAAAAATTAATCACCTGCC  >  NC_000913/3597432‑3597902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑