breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 108,585 C→T R103C (CGT→TGT)  secA → protein translocation ATPase
RA 134,256 G→T intergenic (+44/‑132) acnB → / → yacL AcnB/PF06062 family protein YacL
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
RA 157,043 G→A intergenic (‑160/+210) yadN ← / ← folK putative fimbrial protein YadN/2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine diphosphokinase
JC JC 167,915 IS2 (–) +5 bp coding (432‑436/2244 nt) fhuA → ferrichrome outer membrane transporter/phage receptor
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 287,356 C→T L190L (CTG→TTG)  yagH → putative xylosidase/arabinosidase
RA 289,091 A→G L24L (CTT→CTC xynR ← DNA‑binding transcriptional repressor XynR
RA 306,212 2 bp→AA coding (605‑606/1644 nt) ecpD ← fimbrial adhesin EcpD
RA 322,064 C→T L153L (CTG→TTG)  ykgE → putative lactate utilization oxidoreductase YkgE
RA 338,135 C→T A453V (GCG→GTG)  yahF → putative acyl‑CoA synthetase YahF
JC JC 362,204 IS1 (–) +9 bp coding (968‑976/1254 nt) lacY ← lactose permease
JC JC 432,120 IS1 (+) +9 bp intergenic (‑107/+184) tsx ← / ← yajI nucleoside‑specific channel‑forming protein Tsx/putative lipoprotein YajI
RA 458,188 G→A D255N (GAT→AAT)  clpX → ATP‑dependent Clp protease ATP‑binding subunit ClpX
RA 459,432 C→T S182F (TCT→TTT)  lon → Lon protease
RA 459,572 T→G Y229D (TAT→GAT)  lon → Lon protease
RA 462,394 C→A L160L (CTC→CTA ppiD → periplasmic folding chaperone
RA 534,124 G→C G70A (GGC→GCC)  gcl → glyoxylate carboligase
RA 543,263 2 bp→GT coding (783‑784/786 nt) allE ← (S)‑ureidoglycine aminohydrolase
JC JC 562,453 IS2 (–) +5 bp coding (118‑122/984 nt) sfmH → putative fimbrial adhesin protein SfmH
MC JC 575,786 Δ3,398 bp IS5‑mediated [nmpC]borD [nmpC], essD, rrrD, rzpD, rzoD, borD
RA 628,569 A→G N7D (AAT→GAT)  entA → 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
JC JC 637,849 IS1 (+) +9 bp coding (717‑725/747 nt) dsbG ← protein sulfenic acid reductase and chaperone DsbG
JC JC 646,571 IS5 (+) +4 bp coding (7‑10/1464 nt) citT ← citrate:succinate antiporter
RA 659,517 G→A L234L (CTG→TTG)  lipA ← lipoyl synthase
RA 659,527 G→T T230T (ACC→ACA lipA ← lipoyl synthase
MC JC 696,507 Δ112 bp glnV glnV
RA 727,761 C→T E324E (GAG→GAA kdpA ← K(+) transporting P‑type ATPase subunit KdpA
RA 732,828 T→C G1082G (GGT→GGC rhsC → rhs element protein RhsC
JC JC 735,613 IS5 (+) +4 bp intergenic (+1511/‑34) ybfB → / → ybfC uncharacterized protein YbfB/uncharacterized protein YbfC
RA 740,655 G→A W383* (TGG→TGA phr → deoxyribodipyrimidine photolyase
JC 755,780 Δ7 bp coding (221‑227/348 nt) sdhD → succinate:quinone oxidoreductase, membrane protein SdhD
RA 758,593 T→C intergenic (+188/‑113) sdhB → / → sucA succinate:quinone oxidoreductase, iron‑sulfur cluster binding protein/subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 768,617 G→A E214K (GAG→AAG)  mngB → alpha‑mannosidase
RA 789,853 C→A V43F (GTT→TTT)  galK ← galactokinase
RA 790,086 C→T G315D (GGT→GAT)  galT ← galactose‑1‑phosphate uridylyltransferase
RA 854,975 G→A S257F (TCT→TTT)  ldtB ← L,D‑transpeptidase LdtB
RA 856,434 C→T P158S (CCT→TCT)  ybiT → putative ATP‑binding protein YbiT
RA 857,041 C→A T360K (ACG→AAG)  ybiT → putative ATP‑binding protein YbiT
RA 873,398 Δ1 bp coding (420/2349 nt) pdeI → putative c‑di‑GMP phosphodiesterase PdeI
RA 973,577 C→T S14F (TCC→TTC)  cmoM → tRNA cmo(5)U34 methyltransferase
RA 983,147 C→G P25A (CCG→GCG)  mepK → peptidoglycan L,D‑endopeptidase
RA 985,614 C→G G32G (GGG→GGC aspC ← aspartate aminotransferase
RA 1,005,691 T→A I308I (ATT→ATA pyrD → dihydroorotate dehydrogenase, type 2
RA 1,005,703 C→T A312A (GCC→GCT pyrD → dihydroorotate dehydrogenase, type 2
RA 1,043,607 G→A L202L (CTG→TTG)  etk ← protein‑tyrosine kinase Etk
RA 1,050,092 C→T S87L (TCA→TTA) 
L22L (CTC→CTT
insA4 →
insB4 →
IS1 family protein InsA
IS1 family protein InsB
RA 1,131,522 G→A A29T (GCG→ACG)  flgC → flagellar basal‑body rod protein FlgC
RA 1,146,079 C→A V173F (GTT→TTT)  yceF ← m(7)GTP pyrophosphatase
RA 1,157,902 G→T V12F (GTC→TTC)  ptsG → glucose‑specific PTS enzyme IIBC component
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
JC JC 1,190,337 IS2 (+) +5 bp coding (107‑111/672 nt) phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,195,620 A→G K166K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
JC JC 1,212,703 IS5 (+) +4 bp coding (402‑405/405 nt) ycgX ← DUF1398 domain‑containing protein YcgX
RA 1,220,493 C→T intergenic (+1292/+1812) ymgF → / ← ymgD inner membrane protein YmgF/PF16456 family protein YmgD
RA 1,224,899 G→A R155C (CGT→TGT)  minD ← Z‑ring positioning protein MinD
RA 1,233,669 G→A S350F (TCT→TTT)  nhaB ← Na(+):H(+) antiporter NhaB
RA 1,269,591 G→A intergenic (+202/+112) rdlA → / ← ldrB putative antisense regulatory RNA RdlA/small toxic polypeptide LdrB
RA 1,290,947 C→T Q236* (CAA→TAA)  rssB → regulator of RpoS
RA 1,292,992 A→C intergenic (‑70/‑535) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,463 C→A R234S (CGT→AGT)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,318,275 Δ1 bp coding (141/1194 nt) trpB ← tryptophan synthase subunit beta
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,371,067 C→A G284G (GGC→GGA ycjM → glucosylglycerate phosphorylase
RA 1,374,498 G→T G137C (GGC→TGC)  ycjP → putative ABC transporter membrane subunit YcjP
RA 1,405,758 G→A intergenic (‑7/‑221) mcaS ← / → smrA small regulatory RNA McaS/DNA endonuclease SmrA
RA 1,434,642 G→A intergenic (‑236/+543) ynaM ← / ← uspF protein YnaM/universal stress protein F
RA 1,442,483 G→A H121Y (CAC→TAC)  ldhA ← D‑lactate dehydrogenase
RA 1,531,689 A→G intergenic (‑1185/‑127) yncO ← / → ydcC protein YncO/H repeat‑associated putative transposase YdcC
RA 1,553,630 C→A G70V (GGT→GTT)  adhP ← ethanol dehydrogenase/alcohol dehydrogenase
RA 1,555,731 Δ1 bp intergenic (‑62/+95) maeA ← / ← sra malate dehydrogenase (oxaloacetate‑decarboxylating)/ribosome‑associated protein Sra
RA 1,600,234 C→T intergenic (‑25/+54) lsrK ← / ← lsrR autoinducer‑2 kinase/DNA‑binding transcriptional repressor LsrR
RA 1,601,098 C→A S48S (TCG→TCT lsrR ← DNA‑binding transcriptional repressor LsrR
RA 1,601,103 C→T V47M (GTG→ATG)  lsrR ← DNA‑binding transcriptional repressor LsrR
RA 1,632,712 A→G intergenic (‑427/‑178) ydfJ ← / → ynfT putative transporter YdfJ/protein YnfT
RA 1,632,735 G→A intergenic (‑450/‑155) ydfJ ← / → ynfT putative transporter YdfJ/protein YnfT
RA 1,641,002 T→C intergenic (+27/+337) ynfU → / ← cspB putative zinc‑binding protein YnfU/cold shock‑like protein CspB
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,649,971 C→T P60S (CCG→TCG)  ydfD → lysis protein
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
JC JC 1,652,518 IS2 (+) +5 bp intergenic (+2533/+206) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,697,182 C→T intergenic (‑130/+91) uidR ← / ← hdhA DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase
RA 1,702,757 C→T F175F (TTC→TTT add → adenosine deaminase
MC JC 1,732,136 Δ7,278 bp [lhr][punR] [lhr], grxD, mepH, sodB, ydhP, cydH, purR, [punR]
RA 1,786,745 G→A S123F (TCT→TTT)  ppsA ← phosphoenolpyruvate synthetase
RA 1,793,377 Δ1 bp coding (182/750 nt) btuD ← vitamin B12 ABC transporter ATP binding subunit
RA 1,822,296 G→A intergenic (‑40/‑162) osmE ← / → nadE osmotically‑inducible lipoprotein OsmE/NH3‑dependent NAD(+) synthetase
RA 1,861,311 C→T G8S (GGC→AGC)  ydjL ← putative zinc‑binding dehydrogenase YdjL
RA 1,874,202 G→A intergenic (‑20/‑150) yoaI ← / → yeaL protein YoaI/DUF441 domain‑containing inner membrane protein YeaL
RA 1,882,591 G→A C227Y (TGC→TAC)  dmlA → D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,911,929 2 bp→TT coding (647‑648/882 nt) htpX ← protease HtpX
RA 1,915,132 G→T P135T (CCG→ACG)  proQ ← RNA chaperone ProQ
RA 1,915,945 C→T G62S (GGC→AGC)  msrC ← free methionine‑(R)‑sulfoxide reductase
RA 1,929,595 C→T G38E (GGA→GAA)  yebE ← DUF533 domain‑containing inner membrane protein YebE
RA 1,936,634 C→T intergenic (‑320/‑18) zwf ← / → yebK NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase/DNA‑binding transcriptional repressor YebK
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
JC 2,092,996 (TGGC)4→3 coding (599‑602/1071 nt) hisC → histidinol‑phosphate aminotransferase
RA 2,098,825 C→A A263A (GCG→GCT ugd ← UDP‑glucose 6‑dehydrogenase
RA 2,099,525 A→T I30N (ATT→AAT)  ugd ← UDP‑glucose 6‑dehydrogenase
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,155,880 C→T L206L (CTC→CTT mdtB → multidrug efflux pump RND permease subunit MdtB
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,190,956 G→A D230D (GAC→GAT yehC ← putative fimbrial chaperone YehC
RA 2,191,755 G→A Y156Y (TAC→TAT yehD ← putative fimbrial protein YehD
RA 2,192,782 C→T W5* (TGG→TGA yehE ← DUF2574 domain‑containing protein YehE
RA 2,198,007 C→T intergenic (+709/‑2272) yehF → / → yehI PF05406 family protein YehF/DUF4132 domain‑containing protein YehI
RA 2,199,054 G→A intergenic (+1756/‑1225) yehF → / → yehI PF05406 family protein YehF/DUF4132 domain‑containing protein YehI
RA 2,200,887 G→T L203L (CTG→CTT yehI → DUF4132 domain‑containing protein YehI
RA 2,202,112 C→T L612F (CTC→TTC)  yehI → DUF4132 domain‑containing protein YehI
RA 2,204,047 C→T L26F (CTT→TTT)  yehK → uncharacterized protein YehK
RA 2,205,207 C→T T204T (ACC→ACT yehL → putative AAA(+) MoxR family ATPase YehL
RA 2,207,159 G→A A489T (GCG→ACG)  yehM → uncharacterized protein YehM
RA 2,207,546 G→A G618S (GGC→AGC)  yehM → uncharacterized protein YehM
RA 2,224,144 C→T V243M (GTG→ATG)  pbpG ← peptidoglycan DD‑endopeptidase PbpG
RA 2,224,169 A→G F234F (TTT→TTC pbpG ← peptidoglycan DD‑endopeptidase PbpG
RA 2,227,313 G→A intergenic (‑43/‑1669) yohF ← / → yohP putative oxidoreductase YohF/putative membrane protein YohP
RA 2,228,439 G→A intergenic (‑1169/‑543) yohF ← / → yohP putative oxidoreductase YohF/putative membrane protein YohP
RA 2,230,026 G→A Y120Y (TAC→TAT dusC ← tRNA‑dihydrouridine(16) synthase
RA 2,231,616 G→A E200K (GAA→AAA)  yohK → putative 3‑hydroxypropanoate export protein YohK
RA 2,237,105 G→T P217T (CCG→ACG)  mglC ← D‑galactose/methyl‑galactoside ABC transporter membrane subunit
JC JC 2,237,684 IS5 (+) +4 bp coding (67‑70/1011 nt) mglC ← D‑galactose/methyl‑galactoside ABC transporter membrane subunit
RA 2,264,460 2 bp→AT coding (598‑599/1182 nt) setB → sugar exporter SetB
RA 2,272,997 C→T P212S (CCG→TCG)  yejA → putative oligopeptide ABC transporter periplasmic binding protein YejA
RA 2,287,896 C→T pseudogene (1019/2525 nt) yejO ← adhesin‑like autotransporter YejO
RA 2,296,636 G→A A130V (GCG→GTG)  ccmB ← cytochrome c maturation protein B
RA 2,319,078 C→A D267Y (GAT→TAT)  rcsC ← histidine kinase RcsC
RA 2,360,550 Δ1 bp coding (865/1206 nt) rhmD ← L‑rhamnonate dehydratase
RA 2,392,233 C→G G270A (GGT→GCT)  nuoM ← NADH:quinone oxidoreductase subunit M
RA 2,406,455 G→A R63C (CGC→TGC)  lrhA ← DNA‑binding transcriptional dual regulator LrhA
RA 2,408,858 C→A intergenic (+80/‑4) alaA → / → yfbR glutamate‑‑pyruvate aminotransferase AlaA/dCMP phosphohydrolase
RA 2,423,891 C→T V207I (GTA→ATA)  hisP ← lysine/arginine/ornithine ABC transporter/histidine ABC transporter, ATP binding subunit
RA 2,433,644 G→A L95L (CTG→TTG)  accD ← acetyl‑CoA carboxyltransferase subunit beta
RA 2,443,447 G→A A562T (GCG→ACG)  mnmC → fused tRNA 5‑aminomethyl‑2‑thiouridylate methyltransferase and tRNA 5‑aminomethyl‑2‑thiouridylate synthase
RA 2,473,392 G→A intergenic (‑146/‑212) yfdO ← / → yfdP protein YdfO/uncharacterized protein YfdP
RA 2,473,397 +T intergenic (‑151/‑207) yfdO ← / → yfdP protein YdfO/uncharacterized protein YfdP
RA 2,510,609 C→T P327L (CCG→CTG)  yfeO → putative transport protein YfeO
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,630,852 G→A S176N (AGC→AAC)  yfgI → nalidixic acid resistance protein YfgI
RA 2,632,824 C→T G416D (GGT→GAT)  guaB ← inosine 5'‑monophosphate dehydrogenase
RA 2,680,713 C→T G678E (GGA→GAA)  yphG ← DUF5107 domain‑containing protein YphG
RA 2,684,253 C→T intergenic (+197/+1) yphH → / ← glyA putative DNA‑binding transcriptional regulator, NAGC‑like/serine hydroxymethyltransferase
RA 2,695,324 G→C L74V (CTC→GTC)  purL ← phosphoribosylformylglycinamide synthetase
RA 2,697,981 C→T P190P (CCG→CCA pgpC ← phosphatidylglycerophosphatase C
RA 2,698,371 G→A R60R (CGC→CGT pgpC ← phosphatidylglycerophosphatase C
RA 2,732,992 G→A I394I (ATC→ATT
I394I (ATC→ATT
clpB ←
clpB ←
chaperone protein ClpB
chaperone protein ClpB
RA 2,739,269 Δ1 bp coding (801/1122 nt) tyrA ← fused chorismate mutase/prephenate dehydrogenase
RA 2,804,133 C→T S211F (TCT→TTT)  nrdF → ribonucleoside‑diphosphate reductase 2 subunit beta
RA 2,814,459 Δ1 bp coding (275/516 nt) luxS ← S‑ribosylhomocysteine lyase
RA 2,814,461 C→T L91L (CTG→CTA luxS ← S‑ribosylhomocysteine lyase
RA 2,822,099 G→A intergenic (‑88/+40) alaS ← / ← recX alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor/RecA inhibitor RecX
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,871,153 T→G N51T (AAC→ACC)  truD ← tRNA pseudouridine(13) synthase
RA 2,926,543 2 bp→TT coding (236‑237/1365 nt) ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,981,924 C→T G87R (GGG→AGG)  araE ← arabinose:H(+) symporter
RA 2,982,000 C→T Q61Q (CAG→CAA araE ← arabinose:H(+) symporter
RA 3,040,842 2 bp→AA coding (228‑229/267 nt) sdhE ← FAD assembly factor
RA 3,043,360 G→A I88I (ATC→ATT yqfB ← N(4)‑acetylcytidine amidohydrolase
RA 3,071,412 C→T intergenic (‑168/+47) fbaA ← / ← pgk fructose‑bisphosphate aldolase class II/phosphoglycerate kinase
RA 3,089,109 A→T K276* (AAA→TAA)  galP → galactose:H(+) symporter
MC JC 3,131,341 Δ8,368 bp IS5‑mediated [yghQ]yghU [yghQ], yghR, yghS, yghT, pitB, gss, yghU
RA 3,161,616 G→A R352C (CGC→TGC)  ftsP ← cell division protein FtsP
RA 3,167,566 C→T V51V (GTG→GTA ygiS ← putative deoxycholate binding periplasmic protein
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,242,343 C→T M324I (ATG→ATA uxaA ← D‑altronate dehydratase
RA 3,270,880 G→A S297S (AGC→AGT garK ← glycerate 2‑kinase 1
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
MC JC 3,349,206 Δ16,350 bp IS5‑mediated [mtgA][yhcE] [mtgA], elbB, arcZ, arcB, yhcC, gltB, gltD, gltF, yhcA, yhcD, [yhcE]
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,432,105 C→T L120L (CTG→CTA smg ← DUF494 domain‑containing protein Smg
RA 3,443,961 G→A A46V (GCC→GTC)  secY ← Sec translocon subunit SecY
RA 3,443,980 G→A P40S (CCG→TCG)  secY ← Sec translocon subunit SecY
RA 3,452,022 G→A P89S (CCG→TCG)  rplD ← 50S ribosomal subunit protein L4
RA 3,452,218 G→A F23F (TTC→TTT rplD ← 50S ribosomal subunit protein L4
RA 3,457,378 C→T L334L (CTC→CTT gspD → Type II secretion system protein GspD
RA 3,458,245 A→C S623S (TCA→TCC gspD → Type II secretion system protein GspD
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,494,679 C→T T223T (ACC→ACT nirB → nitrite reductase (NADH) large subunit
RA 3,525,566 T→C intergenic (+143/+23) mrcA → / ← nudE peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA/ADP‑sugar diphosphatase NudE
RA 3,537,999 C→T F205F (TTC→TTT yhgF → putative RNA‑binding protein YhgF
RA 3,555,047 G→T V655F (GTC→TTC)  malT → DNA‑binding transcriptional activator MalT
RA 3,555,241 G→A R719R (CGG→CGA malT → DNA‑binding transcriptional activator MalT
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,606,891 C→T F147F (TTC→TTT zntA → Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
RA 3,617,379 T→A I122K (ATA→AAA)  nikD → Ni(2(+)) ABC transporter ATP binding subunit NikD
RA 3,624,545 T→A D56E (GAT→GAA yhhI → putative transposase
RA 3,647,428 C→T A377V (GCG→GTG)  gor → glutathione reductase (NADPH)
RA 3,679,508 C→T T30T (ACC→ACT kdgK → 2‑dehydro‑3‑deoxygluconokinase
RA 3,686,705 G→A F823F (TTC→TTT bcsC ← cellulose synthase outer membrane channel
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,712,407 G→A G176G (GGC→GGT yhjY ← putative outer membrane protein YhjY
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,955 2 bp→AA coding (810‑811/1323 nt) xylA ← xylose isomerase
JC JC 3,731,420 IS1 (–) +9 bp coding (290‑298/993 nt) xylF → xylose ABC transporter periplasmic binding protein
RA 3,769,447 G→A E35K (GAA→AAA)  yibV → PF15596 family protein YibV
RA 3,773,040 (G)6→4 coding (760‑761/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,783,983 G→A S49F (TCC→TTC)  secB ← protein export chaperone SecB
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,833,622 C→A D306Y (GAC→TAC)  yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,865,885 A→G V130A (GTT→GCT)  yidE ← putative transport protein YidE
RA 3,899,195 C→T E67K (GAA→AAA)  yieK ← putative glucosamine‑6‑phosphate deaminase YieK
RA 3,903,234 G→A V156V (GTC→GTT bglB ← 6‑phospho‑beta‑glucosidase B
RA 3,961,662 G→T G329V (GGC→GTC)  rep → ATP‑dependent DNA helicase Rep
RA 3,965,174 G→A H153Y (CAC→TAC)  rhlB ← ATP‑dependent RNA helicase RhlB
RA 3,968,683 G→A M256I (ATG→ATA rfe → UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
RA 3,969,748 T→C S240P (TCG→CCG)  wzzE → enterobacterial common antigen polysaccharide co‑polymerase
RA 3,999,664 C→T S561L (TCG→TTG)  uvrD → DNA helicase II
RA 4,009,698 G→A A177T (GCA→ACA)  pldB → lysophospholipase L2
RA 4,017,197 C→T intergenic (+5/‑136) udp → / → rmuC uridine phosphorylase/putative recombination limiting protein RmuC
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
MC JC 4,094,351 Δ368 bp intergenic (‑79/+5) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,315,259 C→T G94S (GGC→AGC)  phnO ← aminoalkylphosphonate N‑acetyltransferase
RA 4,315,309 C→T G77D (GGC→GAC)  phnO ← aminoalkylphosphonate N‑acetyltransferase
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,380,105 G→T A138E (GCG→GAG)  frdB ← fumarate reductase iron‑sulfur protein
RA 4,400,254 C→T intergenic (+52/‑34) miaA → / → hfq tRNA dimethylallyltransferase/RNA‑binding protein Hfq
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,475,845 G→A A137T (GCC→ACC)  yjgL → protein YjgL
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,516,350 G→A P110S (CCA→TCA)  fecA ← ferric citrate outer membrane transporter
RA 4,518,142 G→A L32F (CTC→TTC)  fecI ← RNA polymerase sigma factor FecI
RA 4,518,221 G→A A5A (GCC→GCT fecI ← RNA polymerase sigma factor FecI
JC JC 4,542,042 IS1 (+) +8 bp coding (6‑13/597 nt) fimE → regulator for fimA
RA 4,545,746 T→C I217I (ATT→ATC fimD → type I fimbriae usher protein
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196302 1211447 15146 30 [29] [29] 30 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1411925 1433822–1433669 21745–21898 90 [0] [26] 30 [ttcA]–pinR [ttcA],intR,xisR,rcbA,ralA,ralR,recT,recE,racC,ydaE,kilR,kilS,sieB,ydaF,ydaG,racR,ydaS,ydaT,ydaU,ydaV,ydaW,rzoR,trkG,ynaK,ydaY,ynaA,lomR,insH5,lomR,stfR,tfaR,pinR
* * ÷ NC_000913 1434698 1434984 287 30 [29] [7] 97 ynaM/uspF protein YnaM/universal stress protein F
* * ÷ NC_000913 1632742 1633400–1633224 483–659 30 [29] [29] 30 ynfT–ydfK ynfT,ydfK
* * ÷ NC_000913 2947468 2947684 217 68 [0] [29] 30 metW–metV metW,metV
* * ÷ NC_000913 3423824–3424233 3424476–3424239 7–653 30 [29] [29] 30 [rrlD] [rrlD]
* * ÷ NC_000913 3762389–3764074 3765153–3764083 10–2765 30 [29] [29] 30 rhsA rhs element protein RhsA
* * ÷ NC_000913 4038053–4038686 4039164–4038691 6–1112 31 [29] [29] 30 rrlA 23S ribosomal RNA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)133 (1.440) 86/590 0.0 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 14119240 (0.000)90 (1.050) 61/546 0.2 100% coding (25/936 nt) ttcA tRNA cytosine(32) 2‑sulfurtransferase TtcA
?NC_000913 1434985 = 0 (0.000)intergenic (‑579/+200) ynaM/uspF protein YnaM/universal stress protein F
* ? NC_000913 = 29474670 (0.000)68 (1.000) 43/432 0.3 100% intergenic (+4/‑30) metZ/metW tRNA‑Met/tRNA‑Met
?NC_000913 2947688 = 0 (0.000)intergenic (+5/+69) metV/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
* ? NC_000913 4542682 =29 (0.310)126 (1.380) 72/580 0.1 85.7% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 14 (0.150)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269042 (0.450)128 (1.410) 72/580 0.1 82.4% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 14 (0.150)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit