Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,793,377	Δ1 bp	coding (182/750 nt)	btuD ←	vitamin B12 ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,793,377	0	T	.	100.0%	267.5 / NA	54	coding (182/750 nt)	btuD	vitamin B12 ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (35/19);  total (35/19)

CATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAA  >  NC_000913/1793084‑1793672                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                        catcaAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCcgc                                                                                                                                                                                                                                                                                                  >  1:623985/1‑300 (MQ=255)              caATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCtt                                                                                                                                                                                                                                                                                     >  1:395367/1‑300 (MQ=255)                        gCGGGATTGGCTTGTGGTGTGATTTGCAACACCACCGCGGCAAGACGTACGCGTTGCCATTCACCGCCGGAAGGTTGATTGGTGCTACGTCCGAGTTTGTCATCANGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTAcctc                                                                                                                                                                                                                                                                           <  2:641422/300‑3 (MQ=255)                        gCGGGATTGGCTTGTGGTGTGATTTGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGc                                                                                                                                                                                                                                                                           <  1:701046/300‑1 (MQ=255)                           ggATTGGCTTGTGGTGTGATTTGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTACCTTACCGCTgg                                                                                                                                                                                                                                                                        >  1:46893/1‑300 (MQ=255)                                  cTTGTGGTGTGATTTGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTcc                                                                                                                                                                                                                                                                 >  2:718822/1‑300 (MQ=255)                                     gtggtgTGATTTGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGc                                                                                                                                                                                                                                                              >  2:698711/1‑300 (MQ=255)                                            gATTTACAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTcgc                                                                                                                                                                                                                                                       >  2:717132/1‑300 (MQ=255)                                               ttGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTNGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCAGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGcc                                                                                                                                                                                                                                                    >  1:484006/1‑300 (MQ=255)                                                    acacCACCGCAGCAAGACGTACGCGTTGCCATTCACCTCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTTTCATCAAGAGCCAGCGCCCCTGCTACATCATTCAGTAGTTCTGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGTTCATTCCGGCCATTCGCGCCAGTaa                                                                                                                                                                                                                                               <  2:347844/300‑1 (MQ=255)                                                     caccacCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAGGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCCTTCGCGCCAGTAAg                                                                                                                                                                                                                                              >  1:96262/1‑300 (MQ=255)                                                              agcaAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTa                                                                                                                                                                                                                                     <  2:555379/300‑1 (MQ=255)                                                                             ttGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGc                                                                                                                                                                                                                      >  1:637270/1‑300 (MQ=255)                                                                                              aaaGTTGATTGGTGCTACGTCCTAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGAt                                                                                                                                                                                                     <  2:242459/300‑1 (MQ=255)                                                                                                 gTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGATATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGCCCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGACAGTAAGGTACTCTTACCCGCGCCATTCGGCACCACCAGGTGCAGGAtctc                                                                                                                                                                                                  >  2:282894/1‑300 (MQ=255)                                                                                                    gATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCAGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTcccc                                                                                                                                                                                               >  1:626023/1‑300 (MQ=255)                                                                                                         gTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTATGTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGccc                                                                                                                                                                                          <  1:198123/300‑1 (MQ=255)                                                                                                         gTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGccc                                                                                                                                                                                          <  2:721293/300‑1 (MQ=255)                                                                                                         gTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGccc                                                                                                                                                                                          >  1:20756/1‑300 (MQ=255)                                                                                                             tACGTCNTAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTNGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAAc                                                                                                                                                                                      <  2:596052/300‑1 (MQ=255)                                                                                                             tACGTCCTAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAAc                                                                                                                                                                                      <  2:556673/300‑1 (MQ=255)                                                                                                                        ttGTCAGCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGaaa                                                                                                                                                                           <  2:142585/300‑1 (MQ=255)                                                                                                                                   agCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAgg                                                                                                                                                                <  2:192906/300‑1 (MQ=255)                                                                                                                                           ccccTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCATTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCACAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGa                                                                                                                                                        >  2:240804/1‑300 (MQ=255)                                                                                                                                              cTGCGACATCATTCAGTAGTTCGGTACGCGCTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTc                                                                                                                                                     <  2:75196/300‑1 (MQ=255)                                                                                                                                                       catTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATc                                                                                                                                            >  1:47881/1‑300 (MQ=255)                                                                                                                                                       catTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCACCACCAGGTGCAGGATCTCCCCAGCACGAACCTCGACAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATc                                                                                                                                            >  1:21874/1‑300 (MQ=255)                                                                                                                                                        atTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCNCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCt                                                                                                                                           >  1:515178/1‑300 (MQ=255)                                                                                                                                                           cAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCAACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGt                                                                                                                                        >  2:433888/1‑300 (MQ=255)                                                                                                                                                           cAGTAGCTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCAACAGGTGCAGGATCTCACCAGCCCGAACCTCGCCAGAAAGCGGCACCAGGCGGGTAGATTCCGCAACATCTTGt                                                                                                                                        >  2:689689/1‑300 (MQ=255)                                                                                                                                                              tagtTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAAc                                                                                                                                     <  1:298540/300‑1 (MQ=255)                                                                                                                                                              tagtTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGCAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAAc                                                                                                                                     <  2:628890/300‑1 (MQ=255)                                                                                                                                                                     gTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATca                                                                                                                              >  2:410729/1‑300 (MQ=255)                                                                                                                                                                         gcgTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAAt                                                                                                                          >  1:571015/1‑300 (MQ=255)                                                                                                                                                                              ttATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGNAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCNGCAACATCTTGTAACTGCATCACAATAGACa                                                                                                                     <  2:521196/300‑1 (MQ=255)                                                                                                                                                                                      cTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGGTAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTg                                                                                                             >  2:455009/1‑300 (MQ=255)                                                                                                                                                                                         aTGCAGTGTCAGGTAGTGCCAGACCTGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCa                                                                                                          <  2:211941/300‑1 (MQ=255)                                                                                                                                                                                              gtgtCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATCGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCCTTCGCGCCAGTAAGGTACTCTTCACCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCCACATCTTGTAACTGCATCACAATAGACatcgtttcgcaaacgcc                                                                                                     >  2:223631/1‑285 (MQ=255)                                                                                                                                                                                              gtgtCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCCACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGcc                                                                                                     >  2:1799/1‑300 (MQ=255)                                                                                                                                                                                              gtgtCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGcc                                                                                                     >  1:31637/1‑300 (MQ=255)                                                                                                                                                                                              gtgtCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGcc                                                                                                     >  2:267144/1‑300 (MQ=255)                                                                                                                                                                                                gtCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCNTTACCGCTGGTCATTCCGGCCATTCGNGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCGCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAg                                                                                                   >  2:476241/1‑300 (MQ=255)                                                                                                                                                                                                gtCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTACAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTAACCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAg                                                                                                   >  2:202095/1‑300 (MQ=255)                                                                                                                                                                                                   aGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGttt                                                                                                <  1:566727/300‑1 (MQ=255)                                                                                                                                                                                                    ggTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCAAACGCCAGTTTa                                                                                               >  2:383896/1‑300 (MQ=255)                                                                                                                                                                                                    ggTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTACCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTAACCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCAAGTTTa                                                                                               >  2:132620/1‑300 (MQ=255)                                                                                                                                                                                                        gTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCACGAACCTCGCCAGCAAGCGGCCCCAGGCGGGTAGATTCCGAAAAATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGc                                                                                           >  2:690809/1‑300 (MQ=255)                                                                                                                                                                                                                   gCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCGTTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTCTAACAACGCCAGTTTAATGCTTTCCCTcaaa                                                                                >  2:366625/1‑298 (MQ=255)                                                                                                                                                                                                                                         ctgTTTTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCatat                                                          <  1:459100/300‑1 (MQ=255)                                                                                                                                                                                                                                           gttgtGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATcc                                                        >  1:256708/1‑300 (MQ=255)                                                                                                                                                                                                                                              gtgAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCATTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTANCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGNCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCAAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATCCggg                                                     >  2:574665/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gcgcGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTg                                          >  1:147052/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      cgAGTTTTGTTGCGGACCATGCTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCg                             <  1:90282/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           cTTCCAGTGGT‑GCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCGTTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCGTTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCAAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCGTATCCGGGGAAACACGCTGGATGACTTTTCCGTCCCTGCCGACCAGGAAtttt        >  2:620531/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  gtggtGCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCCCCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTNTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCaaaa  >  1:527104/5‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  gtggtGCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCaaaa  >  1:589178/5‑300 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                        CATCAAGAAGCAGCAATTGGCCTGCGGGATTGGCTTGTGGTGTGATTTGCAACACCACCGCAGCAAGACGTACGCGTTGCCATTCACCGCCGGAAAGTTGATTGGTGCTACGTCCGAGTTTGTCATCAAGAGCCAGCGCCCCTGCGACATCATTCAGTAGTTCGGTACGCGTTTTATCGTGCTGATGCAGTGTCAGGTAGTGCCAGACCGGCGTTGCAAACGGCGGCGTCTGCTGTTGTGAAAGATAGGCGCGATGCAGCGCGAGTTTTGTTGCGGACCATGCTTCCAGTGGTTGCCCCGCGAACTGAATGCTTCCCTTACCGCTGGTCATTCCGGCCATTCGCGCCAGTAAGGTACTCTTACCCGCGCCATTCGGCCCCACCAGGTGCAGGATCTCCCCAGCCCGAACCTCGCCAGAAAGCGGCCCCAGGCGGGTAGATTCCGCAACATCTTGTAACTGCATCACAATAGACATTATTTTGCCAACGCCAGTTTAATGCTTTCCATCACAATGGGATCTTCCGGCGTCATATCCGGGGAAAAACGCTGGATGACTTTTCCGTCCCTGCCAACCAGGAATTTTTCAAAA  >  NC_000913/1793084‑1793672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑