Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,227,313	G→A	intergenic (‑43/‑1669)	yohF ← / → yohP	putative oxidoreductase YohF/putative membrane protein YohP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,227,313	0	G	A	100.0%	231.9 / NA	61	intergenic (‑43/‑1669)	yohF/yohP	putative oxidoreductase YohF/putative membrane protein YohP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/32);  total (29/32)

CATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTT  >  NC_000913/2227024‑2227603                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                   caTCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATatcat                                                                                                                                                                                                                                                                                          >  2:611389/1‑300 (MQ=255)               aGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTAccc                                                                                                                                                                                                                                                                            <  1:100996/300‑1 (MQ=255)                gCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTAcccc                                                                                                                                                                                                                                                                           >  2:706279/1‑300 (MQ=255)                      gAATGAGTTTCTCCAGCGCCAGTGCCCCTGCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGccc                                                                                                                                                                                                                                                                     <  2:9392/300‑1 (MQ=255)                            gTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCa                                                                                                                                                                                                                                                               >  1:142851/1‑300 (MQ=255)                                ctcCAGCGCCAGTGCCCCTTCTGTTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAg                                                                                                                                                                                                                                                           <  2:405521/300‑1 (MQ=255)                                     gtgcCAGTGCCCTTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGt                                                                                                                                                                                                                                                      <  2:374904/298‑1 (MQ=255)                                               cccTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGGACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAgg                                                                                                                                                                                                                                            <  2:717085/300‑1 (MQ=255)                                                  ttCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCAt                                                                                                                                                                                                                                         >  2:695957/1‑300 (MQ=255)                                                  ttCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTAATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCAt                                                                                                                                                                                                                                         >  2:135735/1‑300 (MQ=255)                                                    cTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATcc                                                                                                                                                                                                                                       >  1:164097/1‑300 (MQ=255)                                                        tAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATACCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGcc                                                                                                                                                                                                                                   <  1:124981/300‑1 (MQ=255)                                                          gATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCTGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCAc                                                                                                                                                                                                                                 <  1:548851/300‑1 (MQ=255)                                                              tcctagaTCCGGCTGCACGATCTCCGCACGTACTCCGTGGCTAACTACCTCACGCGCGGTACCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGTAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGcc                                                                                                                                                                                                                             <  2:350314/296‑1 (MQ=255)                                                              gCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGcc                                                                                                                                                                                                                             <  2:325696/300‑1 (MQ=255)                                                              gCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGcc                                                                                                                                                                                                                             <  1:594706/300‑1 (MQ=255)                                                                           tGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAg                                                                                                                                                                                                                <  2:710616/300‑1 (MQ=255)                                                                                 aTCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCAtt                                                                                                                                                                                                          >  1:627624/1‑300 (MQ=255)                                                                                   ctcCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTgg                                                                                                                                                                                                        >  1:554311/1‑300 (MQ=255)                                                                                     ccGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGcc                                                                                                                                                                                                      <  2:208903/300‑1 (MQ=255)                                                                                     ccGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACNCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACNTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGcc                                                                                                                                                                                                      <  2:576839/300‑1 (MQ=255)                                                                                     ccGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCGGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCTAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCATTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGcc                                                                                                                                                                                                      <  2:105685/300‑1 (MQ=255)                                                                                        cACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGc                                                                                                                                                                                                   >  2:551343/1‑300 (MQ=255)                                                                                        cACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGc                                                                                                                                                                                                   >  1:201072/1‑300 (MQ=255)                                                                                               gCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCa                                                                                                                                                                                            >  1:411776/1‑300 (MQ=255)                                                                                                ccGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAg                                                                                                                                                                                           <  1:412202/300‑1 (MQ=255)                                                                                                        aaCTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGa                                                                                                                                                                                   <  1:269977/300‑1 (MQ=255)                                                                                                          cTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGAtt                                                                                                                                                                                 >  1:455898/1‑300 (MQ=255)                                                                                                            aCCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCt                                                                                                                                                                               >  2:264711/1‑300 (MQ=255)                                                                                                               tCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGc                                                                                                                                                                            <  1:358534/300‑1 (MQ=255)                                                                                                                                    ccTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTNATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGNCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGc                                                                                                                                                       <  2:686189/300‑1 (MQ=255)                                                                                                                                      ttcttcATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCACCGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGACATTGGCCCGCTCATGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCaa                                                                                                                                                     >  2:12812/1‑300 (MQ=255)                                                                                                                                      ttcttcATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCaa                                                                                                                                                     >  1:719700/1‑300 (MQ=255)                                                                                                                                      ttcttcATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCaa                                                                                                                                                     <  2:234837/300‑1 (MQ=255)                                                                                                                                         ttcATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAatga                                                                                                                                                  <  2:196601/300‑1 (MQ=255)                                                                                                                                           cATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAatgatg                                                                                                                                                >  1:388952/1‑300 (MQ=255)                                                                                                                                           cATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAatgatg                                                                                                                                                <  2:393940/300‑1 (MQ=255)                                                                                                                                                  gTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGt                                                                                                                                         >  1:719179/1‑300 (MQ=255)                                                                                                                                                        ggTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAgc                                                                                                                                   >  1:382167/1‑300 (MQ=255)                                                                                                                                                        ggTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCANCAGCGTTTGTACCCCCCGCCCAACGCNCCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAgc                                                                                                                                   <  2:483728/300‑1 (MQ=255)                                                                                                                                                                   tatCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGTGCCTGCGCa                                                                                                                        >  2:116795/1‑300 (MQ=255)                                                                                                                                                                       aaaCCCNTGCTGCGCCAGTAACAACGCGCACTCTTTGCCGATCCCCGAATCGGANGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGAc                                                                                                                    <  2:606428/300‑1 (MQ=255)                                                                                                                                                                       aaaCCCCTGCTTCACCAGTAATAACGCGCACTCTTTGCCGATCCCCCAACCGTAGTCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCTGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGAc                                                                                                                    <  2:219492/300‑1 (MQ=255)                                                                                                                                                                       aaaCCCCTGCTGCGCCAGTAATAACGCGCACTTTTTGCCAATCCCCTAATCGTAGGCGGTAATAATCGCAACCTGTGCCAGCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCAGCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGAc                                                                                                                    <  2:219607/300‑1 (MQ=255)                                                                                                                                                                       aaaCCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGAc                                                                                                                    <  1:312195/300‑1 (MQ=255)                                                                                                                                                                             ctgctgCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACca                                                                                                              <  2:713935/300‑1 (MQ=255)                                                                                                                                                                                            taaCGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATcgc                                                                                               <  2:326388/300‑1 (MQ=255)                                                                                                                                                                                                     ctctTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCTAGCCCCTGCCCTNGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATCCTGGCTTCGCTGACGCGGGAACCAACAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCCGGGCATCGCGCTCAAttt                                                                                      >  2:167009/1‑300 (MQ=255)                                                                                                                                                                                                     ctctTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCCGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAAttt                                                                                      >  1:645143/1‑300 (MQ=255)                                                                                                                                                                                                     ctctTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAAttt                                                                                      >  1:24494/1‑300 (MQ=255)                                                                                                                                                                                                        tttGCCGATCCCCTAATCGTAGGCGGTAATAATCACAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGTGGATTCTGTCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTgcg                                                                                   <  2:206387/300‑1 (MQ=255)                                                                                                                                                                                                        tttGCCGATCCCCGAATCGTAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTgcg                                                                                   <  2:206817/300‑1 (MQ=255)                                                                                                                                                                                                                tCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGc                                                                           >  1:463104/1‑300 (MQ=255)                                                                                                                                                                                                                                       atCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTg                                                    >  2:411362/1‑300 (MQ=255)                                                                                                                                                                                                                                          gCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAAc                                                 <  1:16152/300‑1 (MQ=255)                                                                                                                                                                                                                                           cAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAAcc                                                <  1:617245/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gtgCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTg                                          >  1:478003/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aCTTAACGCTGAATAAACGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTANAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTGGCTGCCCGCGCCACGTc                         >  1:491619/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 aaCGTTAAGTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAac          >  1:473345/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         gTATAGAAGACGCATATCATCAGTGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGTGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCATGCGCAAGACCTACCACACGCACGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTACGCCAGTGTCTGAACCTGACTGGTTGCCTGCGCCACGTCATTCACCGCTTCAACCACAGCtt  >  2:292640/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         gTATAGAAGACGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTANAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCgatt  >  2:165926/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                   CATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTGCCAGGTAATACCAATATCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTT  >  NC_000913/2227024‑2227603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑