Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,315,309	C→T	G77D (GGC→GAC)	phnO ←	aminoalkylphosphonate N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,315,309	0	C	T	100.0%	210.2 / NA	61	G77D (GGC→GAC)	phnO	aminoalkylphosphonate N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/30);  total (31/30)

GGCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCCGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTCGACCGACT  >  NC_000913/4315022‑4315586                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                      ggCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGa                                                                                                                                                                                                                                                                           >  1:700810/1‑300 (MQ=255)    gcAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACat                                                                                                                                                                                                                                                                        <  1:79162/300‑1 (MQ=255)                    cAGCCCCATGCCGGAATGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCATGCTCATTTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGGTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTg                                                                                                                                                                                                                                                        <  2:26680/300‑1 (MQ=255)                                     gCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCa                                                                                                                                                                                                                                       >  1:277125/1‑300 (MQ=255)                                         tgtgCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGcc                                                                                                                                                                                                                                   <  2:340147/300‑1 (MQ=255)                                          gtgCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCg                                                                                                                                                                                                                                  >  2:332195/1‑300 (MQ=255)                                          gtgCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCg                                                                                                                                                                                                                                  >  1:41127/1‑300 (MQ=255)                                                     gtgccggtgAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGAGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTCACACCAACTCCTAACTTTCGTCGACCTAGTTGCCATAATGCAGATGAAACTGCAAATGCAGGCCGATTATGCCGagaaattagcc                                                                                                                                                                                                                       >  2:566673/1‑290 (MQ=255)                                                                cGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGCCATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAgcg                                                                                                                                                                                                            >  2:562929/1‑300 (MQ=255)                                                                     gggTCAGGCTCATGTTAAAGCGTCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGCTCGGTCCTTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTACTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGCCACCTTCACCATCAAGCAGCGCCAGa                                                                                                                                                                                                       >  2:49914/1‑300 (MQ=255)                                                                      ggTCATGCTCATGTTACAGCGCCTTGGTGAAGCGGAATTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGTTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGAt                                                                                                                                                                                                      <  2:341691/300‑1 (MQ=255)                                                                      ggTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCNTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATNAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGAt                                                                                                                                                                                                      <  2:672536/300‑1 (MQ=255)                                                                      ggTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGAt                                                                                                                                                                                                      <  1:85997/300‑1 (MQ=255)                                                                         cAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTGCGCGCAGATAGAAACGGTGCGCTTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGt                                                                                                                                                                                                   <  2:235741/300‑1 (MQ=255)                                                                              tCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGTGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTAGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTAACACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGTAGCGCCAGATGGTAgtgc                                                                                                                                                                                              >  2:138355/1‑297 (MQ=255)                                                                              tCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGTTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAgcgc                                                                                                                                                                                              <  1:543047/300‑1 (MQ=255)                                                                                        gcgcCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGt                                                                                                                                                                                    <  1:551529/300‑1 (MQ=255)                                                                                        gcgcCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGGGCGCTTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACCTACTGCTGACGTTCAGACCGCGCGCCTGCGGNATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCNAGCAGCGCCAGATGGTAGCACATGTTTGGGt                                                                                                                                                                                    <  2:504214/300‑1 (MQ=255)                                                                                                  gAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTTGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGTTCGCGCAGAtt                                                                                                                                                                          <  2:701081/300‑1 (MQ=255)                                                                                                    aGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTATTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACTGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAATAGCGCCAGATGGTAGCGCATGTGTGGGCCGCGCAGATTgg                                                                                                                                                                        >  2:358314/1‑300 (MQ=255)                                                                                                              gCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAAcc                                                                                                                                                              >  1:111907/1‑300 (MQ=255)                                                                                                                   gCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCAcgc                                                                                                                                                         >  1:181095/1‑300 (MQ=255)                                                                                                                    cTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCAcgcg                                                                                                                                                        >  1:664491/1‑300 (MQ=255)                                                                                                                                     gATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCaa                                                                                                                                       >  1:34372/1‑300 (MQ=255)                                                                                                                                       tAGAGACGGTGCGCGTCGTGGCGCTTCACGTTAGTCGAAAGTTCGGTCATTTCAGCCCCGGCCTGGCGGGCTTCTCCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCGGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAACGCATGTTTGGGTCGCACAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAAc                                                                                                                                     <  2:107169/300‑1 (MQ=255)                                                                                                                                        aGAAACGGTGCGCGTCTTGGCGCTTCACGTTGGTCGAAAGTTCGTTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACt                                                                                                                                    <  2:300630/300‑1 (MQ=255)                                                                                                                                         gAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTc                                                                                                                                   <  1:298581/300‑1 (MQ=255)                                                                                                                                              ggTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTACTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACACATGGTGGCCAAACTCCgact                                                                                                                              >  2:140660/1‑297 (MQ=255)                                                                                                                                                       cgtGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCt                                                                                                                     <  1:216311/300‑1 (MQ=255)                                                                                                                                                                                          tCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTAc                                                                                  <  1:322839/300‑1 (MQ=255)                                                                                                                                                                                               cccGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGt                                                                             <  1:680649/300‑1 (MQ=255)                                                                                                                                                                                                  ggCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTTACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGc                                                                          <  2:393533/300‑1 (MQ=255)                                                                                                                                                                                                   gCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGcc                                                                         >  1:268934/1‑300 (MQ=255)                                                                                                                                                                                                    ccTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTTACGTTCAGACCGCGCGCCTGCTGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCg                                                                        <  2:101079/300‑1 (MQ=255)                                                                                                                                                                                                             cttcttcttCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGNAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt                                                               <  1:509563/300‑1 (MQ=255)                                                                                                                                                                                                             cttcttcttCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt                                                               >  1:247189/1‑300 (MQ=255)                                                                                                                                                                                                                   cttctGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCNTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAg                                                         >  1:519115/1‑300 (MQ=255)                                                                                                                                                                                                                   cctctGCCCACGCCAGTAATTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAGCTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAAATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACGGCGTGGCCGGGCGAAGCTCACAAg                                                         <  2:380760/298‑1 (MQ=255)                                                                                                                                                                                                                      aggCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAg                                                      <  2:707146/298‑1 (MQ=255)                                                                                                                                                                                                                        gCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAgct                                                    >  1:723618/1‑298 (MQ=255)                                                                                                                                                                                                                           gcgagatgtgtataagagacagcGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATg                                                 >  1:339755/23‑300 (MQ=255)                                                                                                                                                                                                                             cGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGgt                                               >  1:576292/1‑300 (MQ=255)                                                                                                                                                                                                                               ccattAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGgtgt                                             >  1:122624/5‑300 (MQ=255)                                                                                                                                                                                                                               ccAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGgtgt                                             >  1:552540/1‑300 (MQ=255)                                                                                                                                                                                                                                    aacctactgcttaCGTTCAGACCGCGCGCCTGCGGCATTGCCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCTAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTtctc                                        <  2:189093/288‑1 (MQ=255)                                                                                                                                                                                                                                    aaCTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTTCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTtctc                                        <  1:330697/300‑1 (MQ=255)                                                                                                                                                                                                                                    aaCTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCAGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTCAACCGCGTCGGTGTCGTACTGCGCGGCCGGGCGAAGCTCACAAGCAGACATGGTGTTtctc                                        >  1:189202/1‑300 (MQ=255)                                                                                                                                                                                                                                     aCTTACTGCTGACGTTGAGACCGCACGCCTGCGGCATTACCACCAACTCCTGAACTTCGTCGATCCAGCTGACATGATACAAATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGACATCAAGCAGCGCCAGATGGTAGCGCATGTTTGAGTCGCGCAGATTGGCGTTACAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCATGTTTTAGCTCACAAATCAGCGCGTAAACCGCATCGGTATCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTGTCTcc                                       >  2:40944/1‑300 (MQ=255)                                                                                                                                                                                                                                     aCTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTtctac                                       >  2:693811/1‑298 (MQ=255)                                                                                                                                                                                                                                     aCTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTcc                                       >  1:461136/1‑300 (MQ=255)                                                                                                                                                                                                                                                 cGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATc                           <  1:307447/300‑1 (MQ=255)                                                                                                                                                                                                                                                 cGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTACTTCTGATGGATc                           >  1:742/1‑300 (MQ=255)                                                                                                                                                                                                                                                 cGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGNTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACNGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTACTTATGATGGATc                           >  2:675057/1‑300 (MQ=255)                                                                                                                                                                                                                                                      aGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGt                      <  2:122144/300‑1 (MQ=255)                                                                                                                                                                                                                                                          cgtgcgcCGGCGGCACTACCCCCCATTCCTGGATTTCGTTGATCCATTTGATATGAGTCAGGTGAAACTGCAAATACAGCCCGATCATGCCGACAACTTCACCATCAATTAGCGCCAGAGGGTATCGTAGGTTTGGGTCGCGCAGATTTGCGTTACAACCCACGCTAAACGCGTGGTGGTCAAACTCCGCCTTTTTTAGCTCACAAATCAGCGCGTAAATCGCGTCGGTGTCGTACTGCGTGGTCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGAGCAGCGTcagc                  <  2:138876/297‑1 (MQ=255)                                                                                                                                                                                                                                                          cgcgcgCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGAGCAGCGTcagc                  <  1:473582/300‑1 (MQ=255)                                                                                                                                                                                                                                                          cgcgcgCCTGCGGCATTACCACCAACTCCTGAACTTCGTCGATCCAGTTTACATNATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGGAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATTGATCAGCGTcagc                  <  2:529344/300‑1 (MQ=255)                                                                                                                                                                                                                                                                ccTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGANGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGANGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCgtg            >  2:670913/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      gCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcgacc      >  1:628739/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        aTTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcgaccga    >  1:413877/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          tACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTCGACCGACt  <  1:588961/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                      GGCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCCGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTCGACCGACT  >  NC_000913/4315022‑4315586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑