Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,525,566	T→C	intergenic (+143/+23)	mrcA → / ← nudE	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA/ADP‑sugar diphosphatase NudE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,525,566	0	T	C	100.0%	337.4 / NA	87	intergenic (+143/+23)	mrcA/nudE	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA/ADP‑sugar diphosphatase NudE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (55/32);  total (55/32)

GAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCT  >  NC_000913/3525276‑3525859                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                      gAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGc                                                                                                                                                                                                                                                                                              >  2:28592/1‑300 (MQ=255)          tcgCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCAc                                                                                                                                                                                                                                                                                     >  1:594488/1‑300 (MQ=255)             cagcaCCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCa                                                                                                                                                                                                                                                                                  <  2:102220/300‑1 (MQ=255)                    gggCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTacac                                                                                                                                                                                                                                                                           <  1:313785/300‑1 (MQ=255)                       cAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGCAAACTCAATAAATTGCAGAAACCCAATAAGCCTGATAAACATTGCGCATCAGGCAACCTTCACGCATTTACACTCg                                                                                                                                                                                                                                                                        >  2:247014/1‑300 (MQ=255)                         gTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑NACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATCAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGcc                                                                                                                                                                                                                                                                      >  2:167826/1‑300 (MQ=255)                         gTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGTAAACTTCACGCATTTACACTCGcc                                                                                                                                                                                                                                                                      >  2:437187/1‑300 (MQ=255)                         gTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGcc                                                                                                                                                                                                                                                                      >  2:439267/1‑300 (MQ=255)                            aGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCt                                                                                                                                                                                                                                                                   >  1:713478/1‑300 (MQ=255)                                aaTGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGNATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGNAAACTTCACGCATTTACACTCGCCCCTGccc                                                                                                                                                                                                                                                               <  2:516205/300‑1 (MQ=255)                                aaTGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCAAGCATTTACACTCGCCCCTGccc                                                                                                                                                                                                                                                               >  2:205887/1‑300 (MQ=255)                                       gCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAAc                                                                                                                                                                                                                                                        <  1:578110/300‑1 (MQ=255)                                             gCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTcg                                                                                                                                                                                                                                                  >  1:115148/1‑300 (MQ=255)                                               cgcgAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTcgcg                                                                                                                                                                                                                                                >  1:211043/1‑300 (MQ=255)                                                  gaagaaTATTCCAGCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGTTGGGAACAGCTATTATCAATAATTGCTATGCACAGTAATTGTTCTGATTAAAAAGGCACTTCGGCGCCTTTTCAGTTTGCTGACGA‑GTGGCACTTGTTTATGCCGGATACGGCGTGAACGCGTGATCCGGCTAACAAAATCGTGAAAACTCAATAAACTGCAGAAACCCCACAAGCCTGATAAACAGTGCGCATCATGCAAACTTCACGCATTTACACTCGCCCCTACCCTTTCAATCATCCGCGCAc                                                                                                                                                                                                                                             <  2:355862/300‑1 (MQ=255)                                                     gagTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTCAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCATGATAAACATTGCGCATCAGGCAAACTTCCCGCATTTCCACTTGCCCCTTCCCTTTCACCCATTCGCGCACGAg                                                                                                                                                                                                                                          >  2:145864/1‑300 (MQ=255)                                                       gTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATGAATTGCAGAAAACCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGACCCTGCCCTTTCAACCCTTCGCGCACGAGGa                                                                                                                                                                                                                                        >  2:182193/1‑300 (MQ=255)                                                         aTTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAAc                                                                                                                                                                                                                                      <  1:294830/300‑1 (MQ=255)                                                              aTCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACTAGGAACAgcgc                                                                                                                                                                                                                                 <  2:358968/300‑1 (MQ=255)                                                                  aaGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTg                                                                                                                                                                                                                             >  2:558381/1‑300 (MQ=255)                                                                  aaGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGAGCACGAGGAACAGCGCACTg                                                                                                                                                                                                                             >  1:356711/1‑300 (MQ=255)                                                                    ggTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGAc                                                                                                                                                                                                                           <  2:132836/300‑1 (MQ=255)                                                                     gTACGCAGCAGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTAACTAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTTGCCCCTGCCCTTTCAACCATTAGCGCACGAGGAATAGCGCACTGACa                                                                                                                                                                                                                          >  2:362713/1‑300 (MQ=255)                                                                      tACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCTAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACAt                                                                                                                                                                                                                         <  1:371058/300‑1 (MQ=255)                                                                      gaCGCAGCCGGCACAACAGGCAGTGCACTAGGTGGGAACGACCATTATCGATAATGTCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTANGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACAt                                                                                                                                                                                                                         <  2:156176/299‑1 (MQ=255)                                                                          cAGCCGACACAACAGTCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGTAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGGTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCCAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTgcg                                                                                                                                                                                                                     <  2:254851/300‑1 (MQ=255)                                                                           aGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTgcgc                                                                                                                                                                                                                    <  2:365956/300‑1 (MQ=255)                                                                            gCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTTTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTgcgcg                                                                                                                                                                                                                   >  2:235198/1‑300 (MQ=255)                                                                            gCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTgcgcg                                                                                                                                                                                                                   >  1:645008/1‑300 (MQ=255)                                                                            gCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACACATTTACACTCGCCCCTGCCCTTTCAACAATTCGCGCACGAGGAACAGCGTACTGACATTgcgcg                                                                                                                                                                                                                   >  2:464764/1‑300 (MQ=255)                                                                                acacAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTc                                                                                                                                                                                                               >  1:409863/1‑300 (MQ=255)                                                                                     acaGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGa                                                                                                                                                                                                          <  1:328696/300‑1 (MQ=255)                                                                                         gCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACGTTGCGCGCTTCATTGAAGTc                                                                                                                                                                                                      >  1:66937/1‑300 (MQ=255)                                                                                         gCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTc                                                                                                                                                                                                      >  1:295005/1‑300 (MQ=255)                                                                                            gTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAgg                                                                                                                                                                                                   >  2:253774/1‑300 (MQ=255)                                                                                            gTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAgg                                                                                                                                                                                                   >  1:596939/1‑300 (MQ=255)                                                                                                   aGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTcc                                                                                                                                                                                            >  1:343549/1‑300 (MQ=255)                                                                                                    ggTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCa                                                                                                                                                                                           >  1:715510/1‑300 (MQ=255)                                                                                                              aCCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCcat                                                                                                                                                                                 >  1:277710/1‑300 (MQ=255)                                                                                                                 aTTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAACAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGTGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGCAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCCACCATTCGCGCACGAGGAACAACGCACTGATATTGCGCGCTTCATTGAAGTCGGGGTCTTCCAGCAAATCcatcat                                                                                                                                                                              >  1:299208/1‑300 (MQ=255)                                                                                                                  ttATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCata                                                                                                                                                                             <  1:102882/300‑1 (MQ=255)                                                                                                                                  ggCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCgccagcggccagc                                                                                                                                                             >  1:236692/1‑300 (MQ=255)                                                                                                                                   gCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCgccagcggccagcg                                                                                                                                                            >  1:665314/1‑300 (MQ=255)                                                                                                                                    cacaGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATATGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAATAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAgcgc                                                                                                                                                           >  2:402803/1‑300 (MQ=255)                                                                                                                                       aGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCAcc                                                                                                                                                        <  1:241493/300‑1 (MQ=255)                                                                                                                                         gAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCtg                                                                                                                                                      >  1:146732/1‑300 (MQ=255)                                                                                                                                            ttGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTgg                                                                                                                                                   <  1:335228/300‑1 (MQ=255)                                                                                                                                               gtCTGATTAAAAAGGCGCTTCGGCGCCTTTTCATTTTGCTGACAAAGTG‑CACTTNTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAg                                                                                                                                                <  2:527040/299‑1 (MQ=255)                                                                                                                                                        aaaaGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTgg                                                                                                                                       >  1:291134/1‑300 (MQ=255)                                                                                                                                                         aaaGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGc                                                                                                                                      <  1:452394/300‑1 (MQ=255)                                                                                                                                                          aaGGCGCTTCTGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCt                                                                                                                                     <  2:330200/300‑1 (MQ=255)                                                                                                                                                          aaGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCt                                                                                                                                     <  1:659284/300‑1 (MQ=255)                                                                                                                                                                cTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCCTCGc                                                                                                                               >  1:419903/1‑300 (MQ=255)                                                                                                                                                                cTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGc                                                                                                                               >  1:397055/1‑300 (MQ=255)                                                                                                                                                                cTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGCGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGCTATTGCGCGCTTCATTGCAGTCAGGGTCTTCCAGCAAATCCATCATATGCGACAGCGGCAAGCGCCCCTGTGGTAGCGGCTCTGGCTCATCGc                                                                                                                               >  1:181202/1‑300 (MQ=255)                                                                                                                                                                       gcCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAg                                                                                                                        <  1:385393/300‑1 (MQ=255)                                                                                                                                                                        ccTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGt                                                                                                                       >  1:307166/1‑300 (MQ=255)                                                                                                                                                                                    gCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTag                                                                                                           >  2:329978/1‑300 (MQ=255)                                                                                                                                                                                       gACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAgaga                                                                                                        <  1:150700/300‑1 (MQ=255)                                                                                                                                                                                             gTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTgc                                                                                                  >  1:732168/1‑300 (MQ=255)                                                                                                                                                                                                 cACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCt                                                                                               >  1:306108/1‑300 (MQ=255)                                                                                                                                                                                                     tGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCCTTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCa                                                                                           >  1:706493/1‑300 (MQ=255)                                                                                                                                                                                                     tGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCa                                                                                           >  1:97263/1‑300 (MQ=255)                                                                                                                                                                                                     cctgcgagatgtgtataagagacaggcgTTATCCGGCTAACAAAATCGCGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCANGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTNCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCa                                                                                           <  2:583754/275‑1 (MQ=255)                                                                                                                                                                                                      gTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCAc                                                                                          >  1:381858/1‑300 (MQ=255)                                                                                                                                                                                                                gATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTTCGGGTAGAGATCTTGCGCTACCACGATATTCAtt                                                                                >  2:118379/1‑300 (MQ=255)                                                                                                                                                                                                                 aTACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCAttt                                                                               >  1:240178/1‑300 (MQ=255)                                                                                                                                                                                                                      gCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTg                                                                          >  2:714805/1‑300 (MQ=255)                                                                                                                                                                                                                      gCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTg                                                                          >  1:615686/1‑300 (MQ=255)                                                                                                                                                                                                                              gcgTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCActgtctcttatacacatctccgagc                                                                  >  1:595473/1‑276 (MQ=255)                                                                                                                                                                                                                              gcgTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTa                                                                  >  1:409951/1‑300 (MQ=255)                                                                                                                                                                                                                                  tATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCATAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACTAGGAACNGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGAc                                                              <  2:163355/300‑1 (MQ=255)                                                                                                                                                                                                                                  tATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGAc                                                              <  1:313489/300‑1 (MQ=255)                                                                                                                                                                                                                                    tcctgCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAGACTTCACGCAGTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCAGTTTGCTGGAAAAGTAAGACgg                                                            <  2:421807/296‑1 (MQ=255)                                                                                                                                                                                                                                          tAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCAt                                                      >  1:538188/1‑300 (MQ=255)                                                                                                                                                                                                                                            aCAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCNCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGc                                                    <  1:501426/300‑1 (MQ=255)                                                                                                                                                                                                                                            aCAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACTAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGc                                                    <  1:611955/300‑1 (MQ=255)                                                                                                                                                                                                                                              aaaaTCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTg                                                  <  1:248595/300‑1 (MQ=255)                                                                                                                                                                                                                                              aaaaTCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCANATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCANTTTGCTGGAAAAGTAAGACGGTGCCATGCTg                                                  <  2:515893/300‑1 (MQ=255)                                                                                                                                                                                                                                                 aTCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGc                                               <  1:213855/300‑1 (MQ=255)                                                                                                                                                                                                                                                  tCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGct                                              <  2:434918/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             ccccATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTc                   >  2:394135/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             ccccATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTc                   >  1:447886/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             ccccATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTAACACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTc                   >  2:403953/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               ccATAAGCCTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCa                 >  1:203894/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       cTGATAAACATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAAc         >  1:209716/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              aCATTGCGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCtcttct  >  2:133849/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                      GAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCAGCCGACACAACAGGCAGTGCACGAGGTGGGAACGACCATTATCGATAATGGCGAGGCACAGGAATTGTTCTGATTAAAAAGGCGCTTCGGCGCCTTTTCAGTTTGCTGACAAAGTG‑CACTTGTTTATGCCGGATACGGCGTGAACGCGTTATCCGGCTAACAAAATCGTGAAAACTCAATAAATTGCAGAAACCCCATAAGCCTGATAAACATTGTGCATCAGGCAAACTTCACGCATTTACACTCGCCCCTGCCCTTTCAACCATTCGCGCACGAGGAACAGCGCACTGACATTGCGCGCTTCATTGAAGTCAGGGTCTTCCAGCAAATCCATCATATGCGCCAGCGGCCAGCGCACCTGTGGTAGCGGCTCTGGCTCATCGCCTTCCAGTGATTCCGGGTAGAGATCTTGCGCTACCACGATATTCATTTTGCTGGAAAAGTAAGACGGTGCCATGCTGAGCTTCTTCAAAAAAGTCAGATCGTTCGCTCCAAATCCAACCTCTTCT  >  NC_000913/3525276‑3525859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑